Restriction map for
pFN21AB3088
(Circular) (Six-base) MAPSORT of: pFN21AB3088.seq Check: 2821 from: 1 to: 5959
pFN21AB3088 5959 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 3302 1937
Size: 955 410 4594
Fragments arranged by size:
4594 955 410
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2743 4022 5406 5674 1632
Size: 1111 1279 1384 268 1917
Fragments arranged by size:
1917 1384 1279 1111 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2175 3095 3702 5274 2175
Size: 920 607 1572 2860
Fragments arranged by size:
2860 1572 920 607
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3084 3284 1870
Size: 73 1141 200 4545
Fragments arranged by size:
4545 1141 200 73
BamHI G'GATC_C
Cuts at: 2479 3289 3656 2479
Size: 810 367 4782
Fragments arranged by size:
4782 810 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2144 2189 2207 2284
Size: 325 217 627 283 74 45 18 77
Cuts at: 2284 2882 3280 4735 618
Size: 598 398 1455 1842
Fragments arranged by size:
1842 1455 627 598 398 325 283 217
77 74 45 18
BanII G_rGCy'C
Cuts at: 1391 2244 3278 1391
Size: 853 1034 4072
Fragments arranged by size:
4072 1034 853
BbeI G_GCGC'C
Cuts at: 2193 2288 2193
Size: 95 5864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BbvCI CC'TCA_GC
Cuts at: 2066 2066
Size: 5959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2422 2551 1548
Size: 874 129 4956
Fragments arranged by size:
4956 874 129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2166 4570 136
Size: 107 122 71 631 605 494 2404 1525
Fragments arranged by size:
2404 1525 631 605 494 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2298 2979 4619 1239
Size: 872 187 681 1640 2579
Fragments arranged by size:
2579 1640 872 681 187
Bpu10I CC'TnA_GC
Cuts at: 2066 2919 2066
Size: 853 5106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2481 4084 4949 5247 2481
Size: 1603 865 298 3193
Fragments arranged by size:
3193 1603 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 2285 3766 4148
Size: 53 83 186 936 657 95 1481 382
Cuts at: 4148 4783 275
Size: 635 1451
Fragments arranged by size:
1481 1451 936 657 635 382 186 95
83 53
BsaWI w'CCGG_w
Cuts at: 2482 4392 4790 5064 5211 5622 2482
Size: 1910 398 274 147 411 2819
Fragments arranged by size:
2819 1910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 1178
Size: 30 165 30 1074 30 4630
Fragments arranged by size:
4630 1074 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2285 2288 2291 1186
Size: 1066 33 3 3 4854
Fragments arranged by size:
4854 1066 33 3 3
BseYI C'CCAG_C
Cuts at: 2213 5162 5658 2213
Size: 2949 496 2514
Fragments arranged by size:
2949 2514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2373 3036 1163
Size: 573 637 663 4086
Fragments arranged by size:
4086 663 637 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2734 4020 4105 5176 2734
Size: 1286 85 1071 3517
Fragments arranged by size:
3517 1286 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2734 3278 4020 4105 5176 1391
Size: 399 454 490 544 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 490 454 399 85
BspEI T'CCGG_A
Cuts at: 2482 5622 2482
Size: 3140 2819
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 3314 844
Size: 1250 1220 3489
Fragments arranged by size:
3489 1250 1220
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2913 3135 4454 4628 65
Size: 1183 1665 222 1319 174 1396
Fragments arranged by size:
1665 1396 1319 1183 222 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 2685 96
Size: 2589 3370
BssHII G'CGCG_C
Cuts at: 1925 2150 1925
Size: 225 5734
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3095 794
Size: 2301 3658
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 2752 1184
Size: 1568 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 3107 3107
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2016 2479 3289 3656 4041 4058 5510 5596
Size: 44 463 810 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 810 463 385 367 346 86
44 17 12
BstZ17I GTA'TAC
Cuts at: 2893 2893
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 2079 2289 513
Size: 552 1014 210 4183
Fragments arranged by size:
4183 1014 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3175 487
Size: 683 56 1949 3271
Fragments arranged by size:
3271 1949 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 2822 3252 3475 4269 4289 2712
Size: 110 430 223 794 20 4382
Fragments arranged by size:
4382 794 430 223 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 3406 4295
Size: 54 1425 50 275 18 328 1248 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1248 889 328 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 3889 1353
Size: 1647 889 3423
Fragments arranged by size:
3423 1647 889
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2298 2743 2979 4022 4619 5406
Size: 393 479 187 445 236 1043 597 787
Cuts at: 5406 5674 1239
Size: 268 1524
Fragments arranged by size:
1524 1043 787 597 479 445 393 268
236 187
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2123
Size: 5959
EcoO109I rG'GnC_Cy
Cuts at: 2172 2182 3161 2172
Size: 10 979 4970
Fragments arranged by size:
4970 979 10
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2193 2288 5106 1678
Size: 515 95 2818 2531
Fragments arranged by size:
2818 2531 515 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2392 2410 2424 2477
Size: 32 135 32 806 14 18 14 53
Cuts at: 2477 2509 2895 2927 4600 4632 4674 4706 1373
Size: 32 386 32 1673 32 42 32 2626
Fragments arranged by size:
2626 1673 806 386 135 53 42 32
32 32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 3303 3549 669
Size: 988 281 289 1076 246 3079
Fragments arranged by size:
3079 1076 988 289 281 246
HindIII A'AGCT_T
Cuts at: 748 2358 2809 748
Size: 1610 451 3898
Fragments arranged by size:
3898 1610 451
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2189 2284 2189
Size: 95 5864
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 2608 5073 5257 1305
Size: 972 331 2465 184 2007
Fragments arranged by size:
2465 2007 972 331 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3917 4276 4435 518
Size: 648 2751 359 159 2042
Fragments arranged by size:
2751 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2195 2291 3375 4052 5200 5445 1606
Size: 475 114 96 1084 677 1148 245 2120
Fragments arranged by size:
2120 1148 1084 677 475 245 114 96
NarI GG'CG_CC
Cuts at: 2190 2285 2190
Size: 95 5864
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2546 3317 4862 1132
Size: 97 1317 771 1545 2229
Fragments arranged by size:
2229 1545 1317 771 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2396 2396
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2172 2182 3161 2172
Size: 10 979 4970
Fragments arranged by size:
4970 979 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 2364 3529 2364
Size: 1165 4794
PspXI vC'TCGA_Gb
Cuts at: 3084 3084
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2046 2106 2124 3259 3311 830
Size: 1216 60 18 1135 52 3478
Fragments arranged by size:
3478 1216 1135 60 52 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacII CC_GC'GG
Cuts at: 2082 2292 2082
Size: 210 5749
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 3050 4207 5919 3050
Size: 1157 1712 3090
Fragments arranged by size:
3090 1712 1157
SfcI C'TryA_G
Cuts at: 826 1045 2042 2102 2120 3255 3307 4442 5123
Size: 219 997 60 18 1135 52 1135 681
Cuts at: 5123 5314 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1135 997 681 219 191 60
52 18
SfoI GGC'GCC
Cuts at: 2191 2286 2191
Size: 95 5864
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2496 3084 4063 4964 5226 820
Size: 197 926 553 588 979 901 262 1553
Fragments arranged by size:
1553 979 926 901 588 553 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 3398 513
Size: 552 2333 3074
Fragments arranged by size:
3074 2333 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2710 3986 4003 4156 4341 4786 325
Size: 1255 1130 1276 17 153 185 445 1498
Fragments arranged by size:
1498 1276 1255 1130 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 3048 4205
Size: 274 80 33 51 1443 708 363 1157
Cuts at: 4205 5917 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1157 708 363 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2814 2846 5919 5951 2814
Size: 32 3073 32 2822
Fragments arranged by size:
3073 2822 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 3084 1943
Size: 1141 4818
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 3153 4088 3153
Size: 935 5024
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PsrI PvuII RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI