Restriction map for
pFN21AB3065
(Circular) (Six-base) MAPSORT of: pFN21AB3065.seq Check: 9609 from: 1 to: 6121
pFN21AB3065 6121 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2517 3464 1937
Size: 580 947 4594
Fragments arranged by size:
4594 947 580
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4184 5568 5836 1632
Size: 2552 1384 268 1917
Fragments arranged by size:
2552 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 2538 2565 2886 2964 3402 5020 5997 1203
Size: 1335 27 321 78 438 1618 977 1327
Fragments arranged by size:
1618 1335 1327 977 438 321 78 27
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3364 1184
Size: 2180 3941
AlwNI CAG_nnn'CTG
Cuts at: 2353 3268 3864 5436 2353
Size: 915 596 1572 3038
Fragments arranged by size:
3038 1572 915 596
ApaI G_GGCC'C
Cuts at: 3077 3077
Size: 6121
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2144 2243 3430 3656 3808 5917 1493
Size: 651 99 1187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 651 226 152 99
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 2939 3442 4897 618
Size: 325 217 627 597 555 503 1455 1842
Fragments arranged by size:
1842 1455 627 597 555 503 325 217
BanII G_rGCy'C
Cuts at: 1391 2078 3077 3440 1391
Size: 687 999 363 4072
Fragments arranged by size:
4072 999 687 363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 928
Size: 971 204 4946
Fragments arranged by size:
4946 971 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 3060 1548
Size: 1512 4609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 4732 136
Size: 107 122 71 631 605 1036 2024 1525
Fragments arranged by size:
2024 1525 1036 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2387 3077 4182 5338 1790
Size: 597 690 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 690 597
BmgBI CAC'GTC
Cuts at: 1318 2967 3405 1318
Size: 1649 438 4034
Fragments arranged by size:
4034 1649 438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2591 3852 4810
Size: 416 53 83 399 24 1174 1261 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1753 1261 1174 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 4781 1239
Size: 1181 2361 2579
Fragments arranged by size:
2579 2361 1181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2071 2539 3912 493
Size: 711 867 468 1373 2702
Fragments arranged by size:
2702 1373 867 711 468
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3228 4554 4952 5226 5373 5784 3228
Size: 1326 398 274 147 411 3565
Fragments arranged by size:
3565 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 3217 1186
Size: 1111 920 4090
Fragments arranged by size:
4090 1111 920
BseYI C'CCAG_C
Cuts at: 2208 2218 2342 2380 3069 5324 5820 2208
Size: 10 124 38 689 2255 496 2509
Fragments arranged by size:
2509 2255 689 496 124 38 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 1163
Size: 573 1548 4000
Fragments arranged by size:
4000 1548 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3362 4182 4267 5338 3362
Size: 820 85 1071 4145
Fragments arranged by size:
4145 1071 820 85
BsmI GAATG_Cn'
Cuts at: 3143 3632 3725 3143
Size: 489 93 5539
Fragments arranged by size:
5539 489 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2387 3077 3362 3440 4182 4267
Size: 399 288 309 690 285 78 742 85
Cuts at: 4267 5338 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 690 399 309 288 285
85 78
BspEI T'CCGG_A
Cuts at: 3228 5784 3228
Size: 2556 3565
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3222 3476 844
Size: 2378 254 3489
Fragments arranged by size:
3489 2378 254
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 4616 4790 65
Size: 1183 1110 2258 174 1396
Fragments arranged by size:
2258 1396 1183 1110 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3317 794
Size: 2523 3598
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2848 2989 3379 1184
Size: 1664 141 390 3926
Fragments arranged by size:
3926 1664 390 141
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 3037 2704
Size: 333 5788
BstYI r'GATC_y
Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116
Size: 1479 367 385 17 1452 86 12 346
Cuts at: 6116 1972
Size: 1977
Fragments arranged by size:
1977 1479 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2613 2703 513
Size: 552 1548 90 3931
Fragments arranged by size:
3931 1548 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 2604 3255 3637 4431 4451 2604
Size: 651 382 794 20 4274
Fragments arranged by size:
4274 794 651 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2566 2601 529
Size: 35 2002 35 4049
Fragments arranged by size:
4049 2002 35 35
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2355 2996 3568
Size: 54 1425 50 275 18 525 641 572
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 641 572 525 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2942 4051 1353
Size: 1589 1109 3423
Fragments arranged by size:
3423 1589 1109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 4184 4781 5568 5836 1239
Size: 393 788 1764 597 787 268 1524
Fragments arranged by size:
1764 1524 788 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2677 2677
Size: 6121
EcoO109I rG'GnC_Cy
Cuts at: 2664 3073 3074 3211 2664
Size: 409 1 137 5574
Fragments arranged by size:
5574 409 137 1
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FspI TGC'GCA
Cuts at: 3261 4627 3261
Size: 1366 4755
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 4762 4794 4836
Size: 32 135 32 530 32 2628 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2628 2626 530 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2357 10
Size: 54 1425 343 525 3774
Fragments arranged by size:
3774 1425 525 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2817 3389 4079 4438 4597 518
Size: 648 1651 572 690 359 159 2042
Fragments arranged by size:
2042 1651 690 648 572 359 159
MspA1I CmG'CkG
Cuts at: 1606 2353 2448 3537 4214 5362 5607 1606
Size: 747 95 1089 677 1148 245 2120
Fragments arranged by size:
2120 1148 1089 747 677 245 95
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5569
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2890 3238 3479 5024 1132
Size: 97 1661 348 241 1545 2229
Fragments arranged by size:
2229 1661 1545 348 241 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 2886 5020 2886
Size: 2134 3987
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 1108
Size: 1245 4876
PfoI T'CCnGG_A
Cuts at: 2397 2397
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PmlI CAC'GTG
Cuts at: 2539 2539
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3211 3211
Size: 6121
PshAI GACnn'nnGTC
Cuts at: 1613 3038 1613
Size: 1425 4696
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PspOMI G'GGCC_C
Cuts at: 3073 3073
Size: 6121
PstI C_TGCA'G
Cuts at: 830 2453 3152 3473 830
Size: 1623 699 321 3478
Fragments arranged by size:
3478 1623 699 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2353 2353
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 2942 2942
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SexAI A'CCwGG_T
Cuts at: 2226 2226
Size: 6121
SfcI C'TryA_G
Cuts at: 826 1045 2449 2518 3148 3469 4604 5285 5476
Size: 219 1404 69 630 321 1135 681 191
Cuts at: 5476 826
Size: 1471
Fragments arranged by size:
1471 1404 1135 681 630 321 219 191
69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 2455 1517
Size: 938 5183
StyI C'CwwG_G
Cuts at: 513 1065 2697 3375 3560 513
Size: 552 1632 678 185 3074
Fragments arranged by size:
3074 1632 678 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 2957 4148 4165 4318 4503 4948
Size: 1255 993 384 1191 17 153 185 445
Cuts at: 4948 325
Size: 1498
Fragments arranged by size:
1498 1255 1191 993 445 384 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4367 6079 96
Size: 274 80 33 51 1443 2390 1712 138
Fragments arranged by size:
2390 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 2306 2338 6081 6113 2130
Size: 32 144 32 3743 32 2138
Fragments arranged by size:
3743 2138 144 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2704 2350
Size: 354 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI