Restriction map for
pFN21AB3061
(Circular) (Six-base) MAPSORT of: pFN21AB3061.seq Check: 2677 from: 1 to: 6370
pFN21AB3061 6370 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3713 1937
Size: 1776 4594
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 2680 3016 3100 3184 3352 3436 3520
Size: 986 62 336 84 84 168 84 84
Cuts at: 3520 4433 5817 6085 1632
Size: 913 1384 268 1917
Fragments arranged by size:
1917 1384 986 913 336 268 168 84
84 84 84 62
AflII C'TTAA_G
Cuts at: 820 1017 2527 820
Size: 197 1510 4663
Fragments arranged by size:
4663 1510 197
AflIII A'CryG_T
Cuts at: 1203 2586 3090 5269 6246 1203
Size: 1383 504 2179 977 1327
Fragments arranged by size:
2179 1383 1327 977 504
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 2739 3411 1166
Size: 1573 672 4125
Fragments arranged by size:
4125 1573 672
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2981 1184
Size: 1797 4573
AlwNI CAG_nnn'CTG
Cuts at: 2269 4113 5685 2269
Size: 1844 1572 2954
Fragments arranged by size:
2954 1844 1572
ApaLI G'TGCA_C
Cuts at: 3152 3372 3430 3476 3656 4427 5583 3152
Size: 220 58 46 180 771 1156 3939
Fragments arranged by size:
3939 1156 771 220 180 58 46
ApoI r'AATT_y
Cuts at: 1493 2730 3679 3905 4057 6166 1493
Size: 1237 949 226 152 2109 1697
Fragments arranged by size:
2109 1697 1237 949 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2402 4924 160
Size: 872 1370 2522 1606
Fragments arranged by size:
2522 1606 1370 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3695 1870
Size: 73 1752 4545
Fragments arranged by size:
4545 1752 73
BamHI G'GATC_C
Cuts at: 2183 2898 3066 3234 3570 3700 4067 2183
Size: 715 168 168 336 130 367 4486
Fragments arranged by size:
4486 715 367 336 168 168 130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3691 5146 618
Size: 325 217 627 1904 1455 1842
Fragments arranged by size:
1904 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2708 2792 3689 1391
Size: 1317 84 897 4072
Fragments arranged by size:
4072 1317 897 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3200 928
Size: 971 1301 4098
Fragments arranged by size:
4098 1301 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4561 4595 1903
Size: 34 2624 34 3678
Fragments arranged by size:
3678 2624 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2657 4262 5472 2657
Size: 1605 1210 3555
Fragments arranged by size:
3555 1605 1210
BclI T'GATC_A
Cuts at: 1548 2452 1548
Size: 904 5466
BfrBI ATG'CAT
Cuts at: 3260 3260
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4981 136
Size: 107 122 71 631 605 3309 1525
Fragments arranged by size:
3309 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2794 6365 2794
Size: 3571 2799
BlpI GC'TnA_GC
Cuts at: 2802 3787 2802
Size: 985 5385
Bme1580I G_kGCm'C
Cuts at: 1790 2111 3156 3363 3376 3434 3480 3660 4431
Size: 321 1045 207 13 58 46 180 771
Cuts at: 4431 5587 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1045 771 321 207 180 58
46 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3000 3336 4101
Size: 416 53 83 399 24 1583 336 765
Cuts at: 4101 5059 442
Size: 958 1753
Fragments arranged by size:
1753 1583 958 765 416 399 336 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2676 5030 1239
Size: 992 445 2354 2579
Fragments arranged by size:
2579 2354 992 445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2407 2867 4495 5360 5658 2407
Size: 460 1628 865 298 3119
Fragments arranged by size:
3119 1628 865 460 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3387 5033 882
Size: 617 1888 1646 2219
Fragments arranged by size:
2219 1888 1646 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 4161 493
Size: 711 1889 1068 2702
Fragments arranged by size:
2702 1889 1068 711
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4177 4559 5194 275
Size: 53 83 186 936 2644 382 635 1451
Fragments arranged by size:
2644 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4803 5201 5475 5622 6033 4803
Size: 398 274 147 411 5140
Fragments arranged by size:
5140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 1178
Size: 30 165 30 783 30 5332
Fragments arranged by size:
5332 783 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 5487
BseYI C'CCAG_C
Cuts at: 2205 5573 6069 2205
Size: 3368 496 2506
Fragments arranged by size:
3368 2506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2695 3115 3199 1163
Size: 573 959 420 84 4334
Fragments arranged by size:
4334 959 573 420 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3820 4581 5609 1540
Size: 275 186 1123 696 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 696 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2708 2792 3156 3376 3434 3480 3555 3660
Size: 629 84 364 220 58 46 75 105
Cuts at: 3660 4431 4516 5587 2079
Size: 771 85 1071 2862
Fragments arranged by size:
2862 1071 771 629 364 220 105 85
84 75 58 46
BsmI GAATG_Cn'
Cuts at: 3881 3974 3881
Size: 93 6277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2111 2708 2792 3156 3363 3376
Size: 399 289 32 597 84 364 207 13
Cuts at: 3376 3434 3480 3555 3660 3689 4431 4516 5587
Size: 58 46 75 105 29 742 85 1071
Cuts at: 5587 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 597 399 364 289 207
105 85 84 75 58 46 32 29
13
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3725 844
Size: 2881 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3126 3210 3232 4257 1394
Size: 429 1303 84 22 1025 3507
Fragments arranged by size:
3507 1303 1025 429 84 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 3019 4865 5039 65
Size: 1183 1771 1846 174 1396
Fragments arranged by size:
1846 1771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 3378 5014 5201 1700
Size: 110 222 1346 1636 187 2869
Fragments arranged by size:
2869 1636 1346 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 2318 96
Size: 2222 4148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 3227 4430 5442 3227
Size: 1203 1012 4155
Fragments arranged by size:
4155 1203 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3522 794
Size: 2728 3642
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 2183 2224 2794 2898 3066 3234 3570 3700
Size: 211 41 570 104 168 168 336 130
Cuts at: 3700 4067 4452 4469 5921 6007 6019 6365 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 570 385 367 346 336 211
168 168 130 104 86 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 3482 3482
Size: 6370
BtgI C'CryG_G
Cuts at: 513 1065 2004 2345 513
Size: 552 939 341 4538
Fragments arranged by size:
4538 939 552 341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 2568 2683 3047 3187 3886 4680 4700 2568
Size: 115 364 140 699 794 20 4238
Fragments arranged by size:
4238 794 699 364 140 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3001 3036 529
Size: 35 2437 35 3863
Fragments arranged by size:
3863 2437 35 35
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DraIII CAC_nnn'GTG
Cuts at: 3151 3151
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3128 5377 809
Size: 507 1812 2249 1802
Fragments arranged by size:
2249 1812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2614 3631 3817
Size: 54 1425 50 275 18 784 1017 186
Cuts at: 3817 4706 6335 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1017 889 784 275 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3817 1537
Size: 275 2005 4090
Fragments arranged by size:
4090 2005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 3035 4300 1353
Size: 899 783 1265 3423
Fragments arranged by size:
3423 1265 899 783
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4957 5331 5477 387
Size: 1064 138 3368 374 146 1280
Fragments arranged by size:
3368 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2706 2790 2706
Size: 84 6286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2618 2676 2680 3016 3100 3184
Size: 393 599 387 58 4 336 84 84
Cuts at: 3184 3352 3436 3520 4433 5030 5817 6085 1239
Size: 168 84 84 913 597 787 268 1524
Fragments arranged by size:
1524 913 787 599 597 393 387 336
268 168 84 84 84 84 58 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2443 2084
Size: 359 6011
EcoO109I rG'GnC_Cy
Cuts at: 2039 2122 2233 2266 2580 3486 2039
Size: 83 111 33 314 906 4923
Fragments arranged by size:
4923 906 314 111 83 33
EcoRI G'AATT_C
Cuts at: 2730 3679 4057 2730
Size: 949 378 5043
Fragments arranged by size:
5043 949 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2497 2074
Size: 423 5947
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 5517 1678
Size: 3839 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2321 2353 2437
Size: 32 135 32 614 32 103 32 84
Cuts at: 2437 2469 2779 2811 5011 5043 5085 5117 1373
Size: 32 310 32 2200 32 42 32 2626
Fragments arranged by size:
2626 2200 614 310 135 103 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3714 3960 669
Size: 988 281 568 1208 246 3079
Fragments arranged by size:
3079 1208 988 568 281 246
HindIII A'AGCT_T
Cuts at: 748 2479 748
Size: 1731 4639
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2834 5484 5668 1305
Size: 1529 2650 184 2007
Fragments arranged by size:
2650 2007 1529 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3633 10
Size: 54 1425 343 1801 2747
Fragments arranged by size:
2747 1801 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2350 2739 2923 3218 3411 3511 4328
Size: 648 1184 389 184 295 193 100 817
Cuts at: 4328 4687 4846 518
Size: 359 159 2042
Fragments arranged by size:
2042 1184 817 648 389 359 295 193
184 159 100
MspA1I CmG'CkG
Cuts at: 1606 2209 2896 3568 3786 4463 5611 5856 1606
Size: 603 687 672 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 672 603 245 218
NaeI GCC'GGC
Cuts at: 2034 2034
Size: 6370
NcoI C'CATG_G
Cuts at: 513 1065 2004 2345 513
Size: 552 939 341 4538
Fragments arranged by size:
4538 939 552 341
NdeI CA'TA_TG
Cuts at: 387 2920 3508 387
Size: 2533 588 3249
Fragments arranged by size:
3249 2533 588
NgoMIV G'CCGG_C
Cuts at: 2032 2032
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NsiI A_TGCA'T
Cuts at: 3262 3262
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 2542 2590 3264 3728 5273 1132
Size: 97 1313 48 674 464 1545 2229
Fragments arranged by size:
2229 1545 1313 674 464 97 48
PasI CC'CwG_GG
Cuts at: 1348 2229 1348
Size: 881 5489
PciI A'CATG_T
Cuts at: 2586 5269 2586
Size: 2683 3687
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6370
PfoI T'CCnGG_A
Cuts at: 2179 2235 2179
Size: 56 6314
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PmlI CAC'GTG
Cuts at: 3093 3093
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 2233 3486 2039
Size: 194 1253 4923
Fragments arranged by size:
4923 1253 194
PshAI GACnn'nnGTC
Cuts at: 1613 2388 1613
Size: 775 5595
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PstI C_TGCA'G
Cuts at: 830 3057 3722 830
Size: 2227 665 3478
Fragments arranged by size:
3478 2227 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2209 2209
Size: 6370
SacI G_AGCT'C
Cuts at: 2708 2792 2708
Size: 84 6286
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SapI GCTCTTCn'nnn_
Cuts at: 2252 2252
Size: 6370
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 3545 4618 6330 3545
Size: 1073 1712 3585
Fragments arranged by size:
3585 1712 1073
SfcI C'TryA_G
Cuts at: 826 1045 2128 2310 3053 3718 4853 5534 5725
Size: 219 1083 182 743 665 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
1471 1135 1083 743 681 665 219 191
182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2386 2527 2846 4474 5375 5637
Size: 197 926 443 141 319 1628 901 262
Cuts at: 5637 820
Size: 1553
Fragments arranged by size:
1628 1553 926 901 443 319 262 197
141
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 2542 3264 3728 1132
Size: 1410 722 464 3774
Fragments arranged by size:
3774 1410 722 464
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2693 3113 3281 3533 1517
Size: 1176 420 168 252 4354
Fragments arranged by size:
4354 1176 420 252 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2345 2361 2439 3809 513
Size: 552 939 341 16 78 1370 3074
Fragments arranged by size:
3074 1370 939 552 341 78 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4397 4414 4567 4752 5197 325
Size: 1255 2817 17 153 185 445 1498
Fragments arranged by size:
2817 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2318 3543 4616
Size: 274 80 33 51 1443 341 1225 1073
Cuts at: 4616 6328 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1225 1073 341 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 3288 3320 6330 6362 2090
Size: 32 1166 32 3010 32 2098
Fragments arranged by size:
3010 2098 1166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6370
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 2693 2861 2945 3029 3113 3197 3281 3533 4499
Size: 168 84 84 84 84 84 252 966
Cuts at: 4499 2693
Size: 4564
Fragments arranged by size:
4564 966 252 168 84 84 84 84
84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV
FseI FspAI KasI NarI NruI PacI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI