Restriction map for
pFN21AB3060
(Circular) (Six-base) MAPSORT of: pFN21AB3060.seq Check: 111 from: 1 to: 6583
pFN21AB3060 6583 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 3771 4710 5083 1563
Size: 2208 939 373 3063
Fragments arranged by size:
3063 2208 939 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2932 3700 4646 6030 6298 1632
Size: 1300 768 946 1384 268 1917
Fragments arranged by size:
1917 1384 1300 946 768 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2348 2390 1184
Size: 1164 42 5377
Fragments arranged by size:
5377 1164 42
AlwNI CAG_nnn'CTG
Cuts at: 2023 2476 2758 3254 4326 5898 2023
Size: 453 282 496 1072 1572 2708
Fragments arranged by size:
2708 1572 1072 496 453 282
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3892 4118 4270 6379 1493
Size: 2399 226 152 2109 1697
Fragments arranged by size:
2399 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 3180 3908 1870
Size: 73 1237 728 4545
Fragments arranged by size:
4545 1237 728 73
BamHI G'GATC_C
Cuts at: 3087 3913 4280 3087
Size: 826 367 5390
Fragments arranged by size:
5390 826 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 2861 2933 3904 5359
Size: 325 217 627 398 676 72 971 1455
Cuts at: 5359 618
Size: 1842
Fragments arranged by size:
1842 1455 971 676 627 398 325 217
72
BanII G_rGCy'C
Cuts at: 1391 3357 3452 3902 1391
Size: 1966 95 450 4072
Fragments arranged by size:
4072 1966 450 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3062 3250 3389 3706 928
Size: 971 1163 188 139 317 3805
Fragments arranged by size:
3805 1163 971 317 188 139
BbvCI CC'TCA_GC
Cuts at: 2487 2487
Size: 6583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4774 4808 1903
Size: 34 2837 34 3678
Fragments arranged by size:
3678 2837 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2916 4475 5685 2916
Size: 1559 1210 3814
Fragments arranged by size:
3814 1559 1210
BclI T'GATC_A
Cuts at: 1548 3874 1548
Size: 2326 4257
BfrBI ATG'CAT
Cuts at: 3531 3531
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5194 136
Size: 107 122 71 631 605 3522 1525
Fragments arranged by size:
3522 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 4000 4000
Size: 6583
Bme1580I G_kGCm'C
Cuts at: 1790 2652 3729 4644 5800 1790
Size: 862 1077 915 1156 2573
Fragments arranged by size:
2573 1156 1077 915 862
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6583
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2155 3119 3353
Size: 416 53 83 399 24 738 964 234
Cuts at: 3353 3644 4314 5272 442
Size: 291 670 958 1753
Fragments arranged by size:
1753 964 958 738 670 416 399 291
234 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3839 3052
Size: 787 5796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5243 1239
Size: 4004 2579
Bpu10I CC'TnA_GC
Cuts at: 2487 2813 3491 2487
Size: 326 678 5579
Fragments arranged by size:
5579 678 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4708 5573 5871 4708
Size: 865 298 5420
Fragments arranged by size:
5420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3023 5246 882
Size: 617 1524 2223 2219
Fragments arranged by size:
2223 2219 1524 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3366 4374 493
Size: 711 918 1244 1008 2702
Fragments arranged by size:
2702 1244 1008 918 711
BsaBI GATnn'nnATC
Cuts at: 2450 6577 2450
Size: 4127 2456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4390 4772 5407 275
Size: 53 83 186 936 2857 382 635 1451
Fragments arranged by size:
2857 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2034 2220 5016 5414 5688 5835 6246 2034
Size: 186 2796 398 274 147 411 2371
Fragments arranged by size:
2796 2371 411 398 274 186 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3756 3786 1178
Size: 30 165 30 2353 30 3975
Fragments arranged by size:
3975 2353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3263 1186
Size: 2077 4506
BseYI C'CCAG_C
Cuts at: 2096 3204 3568 3844 5786 6282 2096
Size: 1108 364 276 1942 496 2397
Fragments arranged by size:
2397 1942 1108 496 364 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3605 3622 1163
Size: 573 1869 17 4124
Fragments arranged by size:
4124 1869 573 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4033 4794 5822 1540
Size: 275 186 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3339 3452 4644 4729 5800 3339
Size: 113 1192 85 1071 4122
Fragments arranged by size:
4122 1192 1071 113 85
BsmI GAATG_Cn'
Cuts at: 4094 4187 4094
Size: 93 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2652 3339 3357 3452 3729 3902 4644
Size: 399 862 687 18 95 277 173 742
Cuts at: 4644 4729 5800 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 862 742 687 399 277 173
95 85 18
BspEI T'CCGG_A
Cuts at: 2034 6246 2034
Size: 4212 2371
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 3041 3938 844
Size: 1478 719 897 3489
Fragments arranged by size:
3489 1478 897 719
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 4470 1394
Size: 429 242 2405 3507
Fragments arranged by size:
3507 2405 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 2325 5078 5252 65
Size: 1183 1077 2753 174 1396
Fragments arranged by size:
2753 1396 1183 1077 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2188 5227 5414 1700
Size: 110 378 3039 187 2869
Fragments arranged by size:
3039 2869 378 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 2110 2501 3536 4643 5655 2110
Size: 391 1035 1107 1012 3038
Fragments arranged by size:
3038 1107 1035 1012 391
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 794
Size: 1229 5354
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2440 2545 2233
Size: 207 105 6271
Fragments arranged by size:
6271 207 105
BstYI r'GATC_y
Cuts at: 1972 3087 3913 4280 4665 4682 6134 6220 6232
Size: 1115 826 367 385 17 1452 86 12
Cuts at: 6232 6578 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1115 826 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 2439 3506 513
Size: 552 939 294 141 1067 3590
Fragments arranged by size:
3590 1067 939 552 294 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5844
Fragments arranged by size:
5844 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 3681 3727 4099 4893 4913 2166
Size: 1515 46 372 794 20 3836
Fragments arranged by size:
3836 1515 794 372 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3025 3887 3025
Size: 862 5721
DraIII CAC_nnn'GTG
Cuts at: 3660 3660
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 3798 4030
Size: 54 1425 50 275 18 519 1449 232
Cuts at: 4030 4919 6548 8
Size: 889 1629 43
Fragments arranged by size:
1629 1449 1425 889 519 275 232 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4030 1537
Size: 275 2218 4090
Fragments arranged by size:
4090 2218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 2589 2951 3077 3281 3830 4513 1353
Size: 1167 69 362 126 204 549 683 3423
Fragments arranged by size:
3423 1167 683 549 362 204 126 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 5170 5544 5690 387
Size: 1064 138 666 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 666 374 146 138
EcoICRI GAG'CTC
Cuts at: 3450 3450
Size: 6583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2932 3700 4646 5243 6030 6298 1239
Size: 393 1300 768 946 597 787 268 1524
Fragments arranged by size:
1524 1300 946 787 768 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2015
Size: 6583
EcoO109I rG'GnC_Cy
Cuts at: 2020 2775 2020
Size: 755 5828
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 6583
FspI TGC'GCA
Cuts at: 2336 5089 2336
Size: 2753 3830
HaeII r_GCGC'y
Cuts at: 1678 5730 1678
Size: 4052 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3022 3054 5224 5256 5298
Size: 32 135 32 1450 32 2170 32 42
Cuts at: 5298 5330 1373
Size: 32 2626
Fragments arranged by size:
2626 2170 1450 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3769 3927 4173 669
Size: 988 281 1831 158 246 3079
Fragments arranged by size:
3079 1831 988 281 246 158
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 2445 2541 2625 5697 5881 1305
Size: 828 312 96 84 3072 184 2007
Fragments arranged by size:
3072 2007 828 312 184 96 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3800 10
Size: 54 1425 343 1968 2793
Fragments arranged by size:
2793 1968 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2438 2444 2621 2975 3158 3536
Size: 648 1119 153 6 177 354 183 378
Cuts at: 3536 4541 4900 5059 518
Size: 1005 359 159 2042
Fragments arranged by size:
2042 1119 1005 648 378 359 354 183
177 159 153 6
MspA1I CmG'CkG
Cuts at: 1606 2985 3999 4676 5824 6069 1606
Size: 1379 1014 677 1148 245 2120
Fragments arranged by size:
2120 1379 1148 1014 677 245
NaeI GCC'GGC
Cuts at: 2190 2190
Size: 6583
NcoI C'CATG_G
Cuts at: 513 1065 2004 2439 513
Size: 552 939 435 4657
Fragments arranged by size:
4657 939 552 435
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NgoMIV G'CCGG_C
Cuts at: 2188 2188
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NsiI A_TGCA'T
Cuts at: 3533 3533
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 2371 3531 3941 5486 1132
Size: 97 1142 1160 410 1545 2229
Fragments arranged by size:
2229 1545 1160 1142 410 97
PasI CC'CwG_GG
Cuts at: 1348 3393 1348
Size: 2045 4538
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3393 1108
Size: 2285 4298
PfoI T'CCnGG_A
Cuts at: 2883 2883
Size: 6583
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PmlI CAC'GTG
Cuts at: 3366 3366
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2775 2775
Size: 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 6551
PstI C_TGCA'G
Cuts at: 830 2019 2179 2472 3935 830
Size: 1189 160 293 1463 3478
Fragments arranged by size:
3478 1463 1189 293 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2985 2985
Size: 6583
SacI G_AGCT'C
Cuts at: 3452 3452
Size: 6583
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 2246 4831 6543 2246
Size: 2585 1712 2286
Fragments arranged by size:
2585 2286 1712
SexAI A'CCwGG_T
Cuts at: 2936 2936
Size: 6583
SfcI C'TryA_G
Cuts at: 826 1045 2015 2175 2468 3931 5066 5747 5938
Size: 219 970 160 293 1463 1135 681 191
Cuts at: 5938 826
Size: 1471
Fragments arranged by size:
1471 1463 1135 970 681 293 219 191
160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3182 3910 1872
Size: 1310 728 4545
Fragments arranged by size:
4545 1310 728
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4687 5588 5850 820
Size: 197 926 2744 901 262 1553
Fragments arranged by size:
2744 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 2371 3531 3941 1132
Size: 1239 1160 410 3774
Fragments arranged by size:
3774 1239 1160 410
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 2108 1517
Size: 591 5992
StyI C'CwwG_G
Cuts at: 513 1065 2004 2439 3262 4022 513
Size: 552 939 435 823 760 3074
Fragments arranged by size:
3074 939 823 760 552 435
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 2721 3365 4610 4627 4780 4965
Size: 1255 557 584 644 1245 17 153 185
Cuts at: 4965 5410 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1245 644 584 557 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2244 2769
Size: 274 80 33 51 1443 165 102 525
Cuts at: 2769 4829 6541 96
Size: 2060 1712 138
Fragments arranged by size:
2060 1712 1443 525 274 165 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6543 6575 6543
Size: 32 6551
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6583
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2437 2266
Size: 171 6412
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3180 3908 1870
Size: 1310 728 4545
Fragments arranged by size:
4545 1310 728
XmnI GAAnn'nnTTC
Cuts at: 3374 4712 3374
Size: 1338 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NarI NruI PacI PspOMI PspXI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI