Restriction map for
pFN21AB3050
(Circular) (Six-base) MAPSORT of: pFN21AB3050.seq Check: 9404 from: 1 to: 6325
pFN21AB3050 6325 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2321 2321
Size: 6325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3668 1937
Size: 1731 4594
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2599 4388 5772 6040 1632
Size: 967 1789 1384 268 1917
Fragments arranged by size:
1917 1789 1384 967 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 2177 3339 5224 6201 1203
Size: 974 1162 1885 977 1327
Fragments arranged by size:
1885 1327 1162 977 974
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2718 1184
Size: 1534 4791
AlwNI CAG_nnn'CTG
Cuts at: 2917 2930 4068 5640 2917
Size: 13 1138 1572 3602
Fragments arranged by size:
3602 1572 1138 13
ApaI G_GGCC'C
Cuts at: 2066 3325 2066
Size: 1259 5066
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2408 3634 3860 4012 6121 1493
Size: 915 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 915 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 2198 2464 3438 3650 1870
Size: 73 255 266 974 212 4545
Fragments arranged by size:
4545 974 266 255 212 73
BamHI G'GATC_C
Cuts at: 2170 3655 4022 2170
Size: 1485 367 4473
Fragments arranged by size:
4473 1485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 3078 3179 3505 3646
Size: 325 217 627 1169 122 101 326 141
Cuts at: 3646 5101 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1169 627 326 325 217 141
122 101
BanII G_rGCy'C
Cuts at: 1391 2066 3222 3325 3410 3644 1391
Size: 675 1156 103 85 234 4072
Fragments arranged by size:
4072 1156 675 234 103 85
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2948 3132 928
Size: 971 1049 184 4121
Fragments arranged by size:
4121 1049 971 184
BbvCI CC'TCA_GC
Cuts at: 2124 2124
Size: 6325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4516 4550 1903
Size: 34 2579 34 3678
Fragments arranged by size:
3678 2579 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 2726 2921
Size: 107 122 71 631 605 463 591 195
Cuts at: 2921 3197 4936 136
Size: 276 1739 1525
Fragments arranged by size:
1739 1525 631 605 591 463 276 195
122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 2687 3742 2687
Size: 1055 5270
Bme1580I G_kGCm'C
Cuts at: 1790 2066 3083 3325 3510 3528 4386 5542 1790
Size: 276 1017 242 185 18 858 1156 2573
Fragments arranged by size:
2573 1156 1017 858 276 242 185 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2443 2534 3161
Size: 416 53 83 399 24 1026 91 627
Cuts at: 3161 4056 5014 442
Size: 895 958 1753
Fragments arranged by size:
1753 1026 958 895 627 416 399 91
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 3429 2803
Size: 626 5699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2753 2795 3039 3632 4985 1239
Size: 1514 42 244 593 1353 2579
Fragments arranged by size:
2579 1514 1353 593 244 42
Bpu10I CC'TnA_GC
Cuts at: 2124 2853 2124
Size: 729 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2975 4450 5315 5613 2117
Size: 858 1475 865 298 2829
Fragments arranged by size:
2829 1475 865 858 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4988 882
Size: 617 3489 2219
Fragments arranged by size:
3489 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4132 4514 5149 275
Size: 53 83 186 936 2599 382 635 1451
Fragments arranged by size:
2599 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4758 5156 5430 5577 5988 4758
Size: 398 274 147 411 5095
Fragments arranged by size:
5095 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2768 2798 3575 3605 1178
Size: 30 165 30 1365 30 777 30 3898
Fragments arranged by size:
3898 1365 777 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2750 2792 2966 1186
Size: 928 636 42 174 4545
Fragments arranged by size:
4545 928 636 174 42
BseYI C'CCAG_C
Cuts at: 2325 2584 3395 5528 6024 2325
Size: 259 811 2133 496 2626
Fragments arranged by size:
2626 2133 811 496 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2212 2585 1163
Size: 573 476 373 4903
Fragments arranged by size:
4903 573 476 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3025 3375 4386 4471 5542 3025
Size: 350 1011 85 1071 3808
Fragments arranged by size:
3808 1071 1011 350 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 3025 3083 3222 3325 3375 3410
Size: 399 276 959 58 139 103 50 35
Cuts at: 3410 3510 3528 3644 4386 4471 5542 1391
Size: 100 18 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 959 742 399 276 139 116
103 100 85 58 50 35 18
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2321 3615 3680 844
Size: 1477 1294 65 3489
Fragments arranged by size:
3489 1477 1294 65
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 2443 4212 1394
Size: 429 292 328 1769 3507
Fragments arranged by size:
3507 1769 429 328 292
BsrDI GCAATG_nn'
Cuts at: 65 1248 3432 4820 4994 65
Size: 1183 2184 1388 174 1396
Fragments arranged by size:
2184 1396 1388 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 4969 5156 1700
Size: 110 272 2887 187 2869
Fragments arranged by size:
2887 2869 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 2170 3655 4022 4407 4424 5876 5962 5974
Size: 198 1485 367 385 17 1452 86 12
Cuts at: 5974 6320 1972
Size: 346 1977
Fragments arranged by size:
1977 1485 1452 385 367 346 198 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2640 2754 3192 513
Size: 552 960 615 114 438 3646
Fragments arranged by size:
3646 960 615 552 438 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 3841 4635 4655 3841
Size: 794 20 5511
Fragments arranged by size:
5511 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DraIII CAC_nnn'GTG
Cuts at: 2455 2455
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2347 2764
Size: 54 1425 50 275 18 192 325 417
Cuts at: 2764 2821 3189 3198 3490 3772 4661 6290 8
Size: 57 368 9 292 282 889 1629 43
Fragments arranged by size:
1629 1425 889 417 368 325 292 282
275 192 57 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3398 4255 1353
Size: 2045 857 3423
Fragments arranged by size:
3423 2045 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2909 4912 5286 5432 387
Size: 1064 138 1320 2003 374 146 1280
Fragments arranged by size:
2003 1320 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2599 2753 2795 3039 3632 4388 4985
Size: 393 967 154 42 244 593 756 597
Cuts at: 4985 5772 6040 1239
Size: 787 268 1524
Fragments arranged by size:
1524 967 787 756 597 593 393 268
244 154 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3350 3611 3350
Size: 261 6064
EcoO109I rG'GnC_Cy
Cuts at: 2062 2063 2104 2128 2202 2740 2750 2914 3321
Size: 1 41 24 74 538 10 164 407
Cuts at: 3321 2062
Size: 5066
Fragments arranged by size:
5066 538 407 164 74 41 24 10
1
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 6325
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 2732 5472 1678
Size: 1054 2740 2531
Fragments arranged by size:
2740 2531 1054
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2768 2800 3577 3609 4966
Size: 32 135 32 1196 32 777 32 1357
Cuts at: 4966 4998 5040 5072 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1357 1196 777 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3669 3915 669
Size: 988 281 1731 246 3079
Fragments arranged by size:
3079 1731 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 3459 3485 5439 5623 1305
Size: 893 1261 26 1954 184 2007
Fragments arranged by size:
2007 1954 1261 893 184 26
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 2766 3492 10
Size: 54 1425 343 192 742 726 2843
Fragments arranged by size:
2843 1425 742 726 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3185 3191 3240 4283 4642 4801 518
Size: 648 2019 6 49 1043 359 159 2042
Fragments arranged by size:
2042 2019 1043 648 359 159 49 6
MspA1I CmG'CkG
Cuts at: 1606 2123 2284 2325 3001 3007 3293 3741 4418
Size: 517 161 41 676 6 286 448 677
Cuts at: 4418 5566 5811 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 676 517 448 286 245
161 41 6
NaeI GCC'GGC
Cuts at: 2084 2084
Size: 6325
NcoI C'CATG_G
Cuts at: 513 1065 2025 2640 513
Size: 552 960 615 4198
Fragments arranged by size:
4198 960 615 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NgoMIV G'CCGG_C
Cuts at: 2082 2082
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 2181 2881 3343 3683 5228 1132
Size: 97 952 700 462 340 1545 2229
Fragments arranged by size:
2229 1545 952 700 462 340 97
PasI CC'CwG_GG
Cuts at: 1348 2046 2677 3361 3511 1348
Size: 698 631 684 150 4162
Fragments arranged by size:
4162 698 684 631 150
PciI A'CATG_T
Cuts at: 2177 3339 5224 2177
Size: 1162 1885 3278
Fragments arranged by size:
3278 1885 1162
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2773 3239 3416 3499 1108
Size: 1665 466 177 83 3934
Fragments arranged by size:
3934 1665 466 177 83
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2202 2104
Size: 98 6227
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PspOMI G'GGCC_C
Cuts at: 2062 3321 2062
Size: 1259 5066
PstI C_TGCA'G
Cuts at: 830 2865 2922 3006 3677 830
Size: 2035 57 84 671 3478
Fragments arranged by size:
3478 2035 671 84 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2284 3001 3007 3293 2284
Size: 717 6 286 5316
Fragments arranged by size:
5316 717 286 6
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SanDI GG'GwC_CC
Cuts at: 2202 2202
Size: 6325
SapI GCTCTTCn'nnn_
Cuts at: 3398 3398
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 2922 3677 2922
Size: 755 5570
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2861 2918 3002 3673 4808 5489 5680
Size: 219 1816 57 84 671 1135 681 191
Cuts at: 5680 826
Size: 1471
Fragments arranged by size:
1816 1471 1135 681 671 219 191 84
57
SfiI GGCCn_nnn'nGGCC
Cuts at: 2135 3197 2135
Size: 1062 5263
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 2200 2466 3652 1872
Size: 328 266 1186 4545
Fragments arranged by size:
4545 1186 328 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2990 4429 5330 5592 820
Size: 197 926 189 858 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 858 262 197 189
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 3212 152
Size: 3060 3265
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 1517
Size: 1203 5122
StyI C'CwwG_G
Cuts at: 513 1065 2025 2640 2721 3764 513
Size: 552 960 615 81 1043 3074
Fragments arranged by size:
3074 1043 960 615 552 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2988 3338 4352 4369 4522 4707 5152
Size: 1255 1408 350 1014 17 153 185 445
Cuts at: 5152 325
Size: 1498
Fragments arranged by size:
1498 1408 1255 1014 445 350 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2488 3030
Size: 274 80 33 51 1443 495 16 542
Cuts at: 3030 4571 6283 96
Size: 1541 1712 138
Fragments arranged by size:
1712 1541 1443 542 495 274 138 80
51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 6285 6317 3575
Size: 32 2678 32 3583
Fragments arranged by size:
3583 2678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3428 1316
Size: 2112 4213
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2647 3190 2647
Size: 543 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 2198 2464 3650 1870
Size: 328 266 1186 4545
Fragments arranged by size:
4545 1186 328 266
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SexAI SfoI SrfI SwaI