Restriction map for
pFN21AB3048
(Circular) (Six-base) MAPSORT of: pFN21AB3048.seq Check: 6634 from: 1 to: 8731
pFN21AB3048 8731 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5807 5807
Size: 8731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3765 6541 278
Size: 53 83 186 936 2229 2776 2468
Fragments arranged by size:
2776 2468 2229 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2667 5386 5430 5666 6074 1937
Size: 730 2719 44 236 408 4594
Fragments arranged by size:
4594 2719 730 408 236 44
Acc65I G'GTAC_C
Cuts at: 2783 3828 4191 6052 2783
Size: 1045 363 1861 5462
Fragments arranged by size:
5462 1861 1045 363
AclI AA'CG_TT
Cuts at: 1563 6858 7231 1563
Size: 5295 373 3063
Fragments arranged by size:
5295 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 3453 4153 5133 5588 5638 6794 8178
Size: 888 933 700 980 455 50 1156 1384
Cuts at: 8178 8446 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1156 980 933 888 700 455
268 50
AfeI AGC'GCT
Cuts at: 2739 2739
Size: 8731
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8534
AflIII A'CryG_T
Cuts at: 1203 4047 4463 4589 5469 7630 8607 1203
Size: 2844 416 126 880 2161 977 1327
Fragments arranged by size:
2844 2161 1327 977 880 416 126
AgeI A'CCGG_T
Cuts at: 7562 7562
Size: 8731
AhdI GACnn_n'nnGTC
Cuts at: 7460 7460
Size: 8731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3547 5164 1184
Size: 2363 1617 4751
Fragments arranged by size:
4751 2363 1617
AlwNI CAG_nnn'CTG
Cuts at: 4941 6474 8046 4941
Size: 1533 1572 5626
Fragments arranged by size:
5626 1572 1533
ApaI G_GGCC'C
Cuts at: 3060 3161 3060
Size: 101 8630
ApaLI G'TGCA_C
Cuts at: 4174 6788 7944 4174
Size: 2614 1156 4961
Fragments arranged by size:
4961 2614 1156
ApoI r'AATT_y
Cuts at: 1493 2296 2456 3910 6004 6040 6266 6418 8527
Size: 803 160 1454 2094 36 226 152 2109
Cuts at: 8527 1493
Size: 1697
Fragments arranged by size:
2109 2094 1697 1454 803 226 160 152
36
AseI AT'TA_AT
Cuts at: 160 1032 2907 4401 4476 7285 160
Size: 872 1875 1494 75 2809 1606
Fragments arranged by size:
2809 1875 1606 1494 872 75
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8731
AvaI C'yCGr_G
Cuts at: 1870 1943 3228 6056 1870
Size: 73 1285 2828 4545
Fragments arranged by size:
4545 2828 1285 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4175 4208 4175
Size: 33 8698
BamHI G'GATC_C
Cuts at: 3001 6061 6428 3001
Size: 3060 367 5304
Fragments arranged by size:
5304 3060 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2783 3828 4191 6052
Size: 325 217 627 546 450 1045 363 1861
Cuts at: 6052 7507 618
Size: 1455 1842
Fragments arranged by size:
1861 1842 1455 1045 627 546 450 363
325 217
BanII G_rGCy'C
Cuts at: 1391 3060 3161 4166 4289 4500 5330 6050 1391
Size: 1669 101 1005 123 211 830 720 4072
Fragments arranged by size:
4072 1669 1005 830 720 211 123 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 2621 3091 4230 928
Size: 971 662 60 470 1139 5429
Fragments arranged by size:
5429 1139 971 662 470 60
BbvCI CC'TCA_GC
Cuts at: 5733 5733
Size: 8731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2961 2995 6922 6956 1903
Size: 34 1024 34 3927 34 3678
Fragments arranged by size:
3927 3678 1024 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 2834 5821 6623 7833 2002
Size: 832 2987 802 1210 2900
Fragments arranged by size:
2987 2900 1210 832 802
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2547 4065 7342
Size: 107 122 71 631 605 875 1518 3277
Cuts at: 7342 136
Size: 1525
Fragments arranged by size:
3277 1525 1518 875 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8726 8726
Size: 8731
BlpI GC'TnA_GC
Cuts at: 5589 6148 5589
Size: 559 8172
Bme1580I G_kGCm'C
Cuts at: 1790 2291 3060 3161 3780 4178 5557 6792 7948
Size: 501 769 101 619 398 1379 1235 1156
Cuts at: 7948 1790
Size: 2573
Fragments arranged by size:
2573 1379 1235 1156 769 619 501 398
101
BmgBI CAC'GTC
Cuts at: 1318 2665 1318
Size: 1347 7384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 4848 5283
Size: 416 53 83 399 24 901 2530 435
Cuts at: 5283 6462 7420 442
Size: 1179 958 1753
Fragments arranged by size:
2530 1753 1179 958 901 435 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3330 5118 5655 6047 7391 1239
Size: 2091 1788 537 392 1344 2579
Fragments arranged by size:
2579 2091 1788 1344 537 392
Bpu10I CC'TnA_GC
Cuts at: 5733 5733
Size: 8731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3341 3459 6856 7721 8019 3341
Size: 118 3397 865 298 4053
Fragments arranged by size:
4053 3397 865 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4329 7394 882
Size: 617 2830 3065 2219
Fragments arranged by size:
3065 2830 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4590 5470 5956 6522 493
Size: 711 3386 880 486 566 2702
Fragments arranged by size:
3386 2702 880 711 566 486
BsaBI GATnn'nnATC
Cuts at: 4884 5817 8725 4884
Size: 933 2908 4890
Fragments arranged by size:
4890 2908 933
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3762 4932 5617 5640
Size: 53 83 186 936 2229 1170 685 23
Cuts at: 5640 6538 6920 7555 275
Size: 898 382 635 1451
Fragments arranged by size:
2229 1451 1170 936 898 685 635 382
186 83 53 23
BsaWI w'CCGG_w
Cuts at: 3804 4081 4432 5880 7164 7562 7836 7983 8394
Size: 277 351 1448 1284 398 274 147 411
Cuts at: 8394 3804
Size: 4141
Fragments arranged by size:
4141 1448 1284 411 398 351 277 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2436 2466 3391 3421 3629
Size: 30 165 30 1033 30 925 30 208
Cuts at: 3629 3659 4063 4093 1178
Size: 30 404 30 5816
Fragments arranged by size:
5816 1033 925 404 208 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3731 4404 5735 1186
Size: 2545 673 1331 4182
Fragments arranged by size:
4182 2545 1331 673
BseYI C'CCAG_C
Cuts at: 2364 3336 3694 5445 7934 8430 2364
Size: 972 358 1751 2489 496 2665
Fragments arranged by size:
2665 2489 1751 972 496 358
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2104 2284 3255 3925 4291 5965 1163
Size: 573 368 180 971 670 366 1674 3929
Fragments arranged by size:
3929 1674 971 670 573 368 366 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5393 6181 6942 7970 1540
Size: 275 186 3392 788 761 1028 2301
Fragments arranged by size:
3392 2301 1028 788 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4178 4261 6792 6877 7948 4178
Size: 83 2531 85 1071 4961
Fragments arranged by size:
4961 2531 1071 85 83
BsiWI C'GTAC_G
Cuts at: 4586 4586
Size: 8731
BsmI GAATG_Cn'
Cuts at: 2359 2864 3752 4241 4364 4916 4985 5153 5219
Size: 505 888 489 123 552 69 168 66
Cuts at: 5219 5744 5867 5905 6242 6335 2359
Size: 525 123 38 337 93 4755
Fragments arranged by size:
4755 888 552 525 505 489 337 168
123 123 93 69 66 38
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2291 3060 3161 3780 4166 4178 4261
Size: 399 501 769 101 619 386 12 83
Cuts at: 4261 4289 4500 5330 5557 6050 6792 6877 7948
Size: 28 211 830 227 493 742 85 1071
Cuts at: 7948 1391
Size: 2174
Fragments arranged by size:
2174 1071 830 769 742 619 501 493
399 386 227 211 101 85 83 28
12
BspEI T'CCGG_A
Cuts at: 4081 4432 8394 4081
Size: 351 3962 4418
Fragments arranged by size:
4418 3962 351
BspHI T'CATG_A
Cuts at: 6620 8350 6620
Size: 1730 7001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 2641 2987 5807 6086 844
Size: 1282 515 346 2820 279 3489
Fragments arranged by size:
3489 2820 1282 515 346 279
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 4851 6618 1394
Size: 429 230 2798 1767 3507
Fragments arranged by size:
3507 2798 1767 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 7226 7400 65
Size: 1183 5978 174 1396
Fragments arranged by size:
5978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 5392 5478 5619 7375 7562 1700
Size: 110 887 2695 86 141 1756 187 2869
Fragments arranged by size:
2869 2695 1756 887 187 141 110 86
BsrGI T'GTAC_A
Cuts at: 96 4988 96
Size: 4892 3839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8731
BssSI C'ACGA_G
Cuts at: 6791 7803 6791
Size: 1012 7719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8731
BstBI TT'CG_AA
Cuts at: 7535 7535
Size: 8731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8731
BstXI CCAn_nnnn'nTGG
Cuts at: 5362 5362
Size: 8731
BstYI r'GATC_y
Cuts at: 1972 2319 3001 3920 6061 6428 6813 6830 8282
Size: 347 682 919 2141 367 385 17 1452
Cuts at: 8282 8368 8380 8726 1972
Size: 86 12 346 1977
Fragments arranged by size:
2141 1977 1452 919 682 385 367 347
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 5387 5667 5387
Size: 280 8451
Bsu36I CC'TnA_GG
Cuts at: 3752 3752
Size: 8731
BtgI C'CryG_G
Cuts at: 513 1065 2004 2329 2673 2958 5355 513
Size: 552 939 325 344 285 2397 3889
Fragments arranged by size:
3889 2397 939 552 344 325 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5880 487
Size: 683 56 4654 3338
Fragments arranged by size:
4654 3338 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 3908 4151 4274 4850 5264 5555 6247 7041
Size: 379 243 123 576 414 291 692 794
Cuts at: 7041 7061 3529
Size: 20 5199
Fragments arranged by size:
5199 794 692 576 414 379 291 243
123 20
ClaI AT'CG_AT
Cuts at: 1554 2971 1554
Size: 1417 7314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8696
DraI TTT'AAA
Cuts at: 6035 6035
Size: 8731
DraIII CAC_nnn'GTG
Cuts at: 2729 3167 5514 2729
Size: 438 2347 5946
Fragments arranged by size:
5946 2347 438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7738 809
Size: 507 6422 1802
Fragments arranged by size:
6422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2208 2257
Size: 54 1425 50 275 18 244 134 49
Cuts at: 2257 2695 3708 4582 5305 5832 6178 7067 8696
Size: 438 1013 874 723 527 346 889 1629
Cuts at: 8696 8
Size: 43
Fragments arranged by size:
1629 1425 1013 889 874 723 527 438
346 275 244 134 54 50 49 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6178 1537
Size: 275 4366 4090
Fragments arranged by size:
4366 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3042 3195 6661 1353
Size: 1689 153 3466 3423
Fragments arranged by size:
3466 3423 1689 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3266 7318 7692 7838 387
Size: 1064 138 1677 4052 374 146 1280
Fragments arranged by size:
4052 1677 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2520 3330 3453 4153 5118 5133 5588
Size: 393 888 810 123 700 965 15 455
Cuts at: 5588 5638 5655 6047 6794 7391 8178 8446 1239
Size: 50 17 392 747 597 787 268 1524
Fragments arranged by size:
1524 965 888 810 787 747 700 597
455 393 392 268 123 50 17 15
EcoO109I rG'GnC_Cy
Cuts at: 3056 3057 3157 3214 3798 4413 4788 3056
Size: 1 100 57 584 615 375 6999
Fragments arranged by size:
6999 615 584 375 100 57 1
EcoRI G'AATT_C
Cuts at: 3910 6040 6418 3910
Size: 2130 378 6223
Fragments arranged by size:
6223 2130 378
EcoRV GAT'ATC
Cuts at: 3514 4351 3514
Size: 837 7894
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 8731
FspI TGC'GCA
Cuts at: 4012 7237 4012
Size: 3225 5506
HaeII r_GCGC'y
Cuts at: 1678 2741 7878 1678
Size: 1063 5137 2531
Fragments arranged by size:
5137 2531 1063
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5154 5186 7372 7404 7446
Size: 32 135 32 3582 32 2186 32 42
Cuts at: 7446 7478 1373
Size: 32 2626
Fragments arranged by size:
3582 2626 2186 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3332 3742 5650 6075 6321 669
Size: 988 281 1394 410 1908 425 246 3079
Fragments arranged by size:
3079 1908 1394 988 425 410 281 246
HindIII A'AGCT_T
Cuts at: 748 2502 3435 748
Size: 1754 933 6044
Fragments arranged by size:
6044 1754 933
HpaI GTT'AAC
Cuts at: 6321 6321
Size: 8731
KpnI G_GTAC'C
Cuts at: 2787 3832 4195 6056 2787
Size: 1045 363 1861 5462
Fragments arranged by size:
5462 1861 1045 363
MfeI C'AATT_G
Cuts at: 6330 6330
Size: 8731
MluI A'CGCG_T
Cuts at: 8607 8607
Size: 8731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3658 4053 7845 8029 1305
Size: 2353 395 3792 184 2007
Fragments arranged by size:
3792 2353 2007 395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2259 3710 5307 10
Size: 54 1425 343 427 1451 1597 3434
Fragments arranged by size:
3434 1597 1451 1425 427 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 4254 5063 5457 5606 6689 7048
Size: 648 918 2170 809 394 149 1083 359
Cuts at: 7048 7207 518
Size: 159 2042
Fragments arranged by size:
2170 2042 1083 918 809 648 394 359
159 149
MspA1I CmG'CkG
Cuts at: 1606 2133 2145 3340 3657 3825 4198 4658 4671
Size: 527 12 1195 317 168 373 460 13
Cuts at: 4671 5593 6147 6824 7972 8217 1606
Size: 922 554 677 1148 245 2120
Fragments arranged by size:
2120 1195 1148 922 677 554 527 460
373 317 245 168 13 12
NaeI GCC'GGC
Cuts at: 5480 5621 5480
Size: 141 8590
NcoI C'CATG_G
Cuts at: 513 1065 2004 2329 2958 5355 513
Size: 552 939 325 629 2397 3889
Fragments arranged by size:
3889 2397 939 629 552 325
NdeI CA'TA_TG
Cuts at: 387 5629 387
Size: 5242 3489
NgoMIV G'CCGG_C
Cuts at: 5478 5619 5478
Size: 141 8590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8731
NotI GC'GGCC_GC
Cuts at: 6178 6178
Size: 8731
NspI r_CATG'y
Cuts at: 1132 1229 2396 4051 4467 5548 6089 7634 1132
Size: 97 1167 1655 416 1081 541 1545 2229
Fragments arranged by size:
2229 1655 1545 1167 1081 541 416 97
PasI CC'CwG_GG
Cuts at: 1348 3649 1348
Size: 2301 6430
PciI A'CATG_T
Cuts at: 4047 4463 7630 4047
Size: 416 3167 5148
Fragments arranged by size:
5148 3167 416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2330 2854 4706 1108
Size: 1222 524 1852 5133
Fragments arranged by size:
5133 1852 1222 524
PfoI T'CCnGG_A
Cuts at: 2706 3916 4853 5361 5784 2706
Size: 1210 937 508 423 5653
Fragments arranged by size:
5653 1210 937 508 423
PmeI GTTT'AAAC
Cuts at: 6035 6035
Size: 8731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6800 6832 8313 8345 6800
Size: 32 1481 32 7186
Fragments arranged by size:
7186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4413 4788 4413
Size: 375 8356
PshAI GACnn'nnGTC
Cuts at: 1613 2280 2393 4935 5846 1613
Size: 667 113 2542 911 4498
Fragments arranged by size:
4498 2542 911 667 113
PsiI TTA'TAA
Cuts at: 2874 6301 2874
Size: 3427 5304
PspOMI G'GGCC_C
Cuts at: 3056 3157 3056
Size: 101 8630
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2767 2799 2767
Size: 32 8699
PstI C_TGCA'G
Cuts at: 830 4411 4579 5259 6083 830
Size: 3581 168 680 824 3478
Fragments arranged by size:
3581 3478 824 680 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8731
PvuII CAG'CTG
Cuts at: 2133 2145 3340 4198 4658 5593 2133
Size: 12 1195 858 460 935 5271
Fragments arranged by size:
5271 1195 935 858 460 12
SalI G'TCGA_C
Cuts at: 1936 6073 1936
Size: 4137 4594
SapI GCTCTTCn'nnn_
Cuts at: 3195 3195
Size: 8731
SbfI CC_TGCA'GG
Cuts at: 6083 6083
Size: 8731
ScaI AGT'ACT
Cuts at: 6979 8691 6979
Size: 1712 7019
SfcI C'TryA_G
Cuts at: 826 1045 3577 4096 4407 4575 5255 6079 7214
Size: 219 2532 519 311 168 680 824 1135
Cuts at: 7214 7895 8086 826
Size: 681 191 1471
Fragments arranged by size:
2532 1471 1135 824 681 680 519 311
219 191 168
SfiI GGCCn_nnn'nGGCC
Cuts at: 2547 2547
Size: 8731
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8731
SmaI CCC'GGG
Cuts at: 1872 3230 6058 1872
Size: 1358 2828 4545
Fragments arranged by size:
4545 2828 1358
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3320 3474 6835 7736 7998 820
Size: 197 926 1377 154 3361 901 262 1553
Fragments arranged by size:
3361 1553 1377 926 901 262 197 154
SnaBI TAC'GTA
Cuts at: 493 6522 493
Size: 6029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8731
SphI G_CATG'C
Cuts at: 1132 5548 6089 1132
Size: 4416 541 3774
Fragments arranged by size:
4416 3774 541
SspI AAT'ATT
Cuts at: 5 52 6655 5
Size: 47 6603 2081
Fragments arranged by size:
6603 2081 47
StuI AGG'CCT
Cuts at: 1517 3152 5537 1517
Size: 1635 2385 4711
Fragments arranged by size:
4711 2385 1635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2329 2596 2958 3195 5355
Size: 552 939 120 205 267 362 237 2160
Cuts at: 5355 5643 6170 513
Size: 288 527 3074
Fragments arranged by size:
3074 2160 939 552 527 362 288 267
237 205 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6758 6775 6928 7113 7558 325
Size: 1255 5178 17 153 185 445 1498
Fragments arranged by size:
5178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3497 3623 4424
Size: 274 80 33 51 1443 1520 126 801
Cuts at: 4424 4824 4988 5345 6977 8689 96
Size: 400 164 357 1632 1712 138
Fragments arranged by size:
1712 1632 1520 1443 801 400 357 274
164 138 126 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 3629 3661 4117 4149 8691 8723 2436
Size: 32 1161 32 456 32 4542 32 2444
Fragments arranged by size:
4542 2444 1161 456 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8731
XbaI T'CTAG_A
Cuts at: 6067 6067
Size: 8731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2714 2714
Size: 8731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8731
XmaI C'CCGG_G
Cuts at: 1870 3228 6056 1870
Size: 1358 2828 4545
Fragments arranged by size:
4545 2828 1358
XmnI GAAnn'nnTTC
Cuts at: 6860 6860
Size: 8731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3763 6539 276
Size: 53 83 186 936 2229 2776 2468
Fragments arranged by size:
2776 2468 2229 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BplI BsmBI EcoICRI
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI RsrII SacI SacII SanDI SexAI SfoI
SrfI SwaI