Restriction map for
pFN21AB3044
(Circular) (Six-base) MAPSORT of: pFN21AB3044.seq Check: 3985 from: 1 to: 6577
pFN21AB3044 6577 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4387 278
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2389 2521 3291 3920 1937
Size: 452 132 770 629 4594
Fragments arranged by size:
4594 770 629 452 132
Acc65I G'GTAC_C
Cuts at: 3898 3898
Size: 6577
AclI AA'CG_TT
Cuts at: 1563 3826 4704 5077 1563
Size: 2263 878 373 3063
Fragments arranged by size:
3063 2263 878 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 4640 6024 6292 1632
Size: 564 2444 1384 268 1917
Fragments arranged by size:
2444 1917 1384 564 268
AfeI AGC'GCT
Cuts at: 2081 2081
Size: 6577
AflII C'TTAA_G
Cuts at: 820 1017 2897 820
Size: 197 1880 4500
Fragments arranged by size:
4500 1880 197
AflIII A'CryG_T
Cuts at: 1203 2633 2692 5476 6453 1203
Size: 1430 59 2784 977 1327
Fragments arranged by size:
2784 1430 1327 977 59
AgeI A'CCGG_T
Cuts at: 5408 5408
Size: 6577
AhdI GACnn_n'nnGTC
Cuts at: 3374 5306 3374
Size: 1932 4645
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6577
AlwNI CAG_nnn'CTG
Cuts at: 4320 5892 4320
Size: 1572 5005
ApaLI G'TGCA_C
Cuts at: 4634 5790 4634
Size: 1156 5421
ApoI r'AATT_y
Cuts at: 1493 3815 3886 4112 4264 6373 1493
Size: 2322 71 226 152 2109 1697
Fragments arranged by size:
2322 2109 1697 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 2712 2955 5131 160
Size: 872 1680 243 2176 1606
Fragments arranged by size:
2176 1680 1606 872 243
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
AvaI C'yCGr_G
Cuts at: 1870 1943 3902 1870
Size: 73 1959 4545
Fragments arranged by size:
4545 1959 73
BamHI G'GATC_C
Cuts at: 2047 2204 2305 3328 3907 4274 2047
Size: 157 101 1023 579 367 4350
Fragments arranged by size:
4350 1023 579 367 157 101
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3769 3898 5353 618
Size: 325 217 627 1982 129 1455 1842
Fragments arranged by size:
1982 1842 1455 627 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2039 3896 1391
Size: 648 1857 4072
Fragments arranged by size:
4072 1857 648
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3132 928
Size: 971 1233 4373
Fragments arranged by size:
4373 1233 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4768 4802 1903
Size: 34 2831 34 3678
Fragments arranged by size:
3678 2831 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3819 4469 5679 3819
Size: 650 1210 4717
Fragments arranged by size:
4717 1210 650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6577
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5188 136
Size: 107 122 71 631 605 3516 1525
Fragments arranged by size:
3516 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6572 6572
Size: 6577
BlpI GC'TnA_GC
Cuts at: 3994 3994
Size: 6577
Bme1580I G_kGCm'C
Cuts at: 1790 3772 4638 5794 1790
Size: 1982 866 1156 2573
Fragments arranged by size:
2573 1982 1156 866
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6577
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3424 4308 5266
Size: 416 53 83 399 24 2007 884 958
Cuts at: 5266 442
Size: 1753
Fragments arranged by size:
2007 1753 958 884 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2439 3090 3780 5237 1239
Size: 1200 651 690 1457 2579
Fragments arranged by size:
2579 1457 1200 690 651
Bpu10I CC'TnA_GC
Cuts at: 2301 2301
Size: 6577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4702 5567 5865 4702
Size: 865 298 5414
Fragments arranged by size:
5414 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5240 882
Size: 617 3741 2219
Fragments arranged by size:
3741 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2477 4368 493
Size: 711 1273 1891 2702
Fragments arranged by size:
2702 1891 1273 711
BsaBI GATnn'nnATC
Cuts at: 2310 2368 2374 6571 2310
Size: 58 6 4197 2316
Fragments arranged by size:
4197 2316 58 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3360 4384 4766 5401
Size: 53 83 186 936 1827 1024 382 635
Cuts at: 5401 275
Size: 1451
Fragments arranged by size:
1827 1451 1024 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5010 5408 5682 5829 6240 5010
Size: 398 274 147 411 5347
Fragments arranged by size:
5347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6352
Fragments arranged by size:
6352 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 3609 1186
Size: 871 1552 4154
Fragments arranged by size:
4154 1552 871
BseYI C'CCAG_C
Cuts at: 5780 6276 5780
Size: 496 6081
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3227 1163
Size: 573 1491 4513
Fragments arranged by size:
4513 1491 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4027 4788 5816 1540
Size: 275 186 2026 761 1028 2301
Fragments arranged by size:
2301 2026 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2302 3619 4638 4723 5794 2302
Size: 1317 1019 85 1071 3085
Fragments arranged by size:
3085 1317 1071 1019 85
BsmI GAATG_Cn'
Cuts at: 4088 4181 4088
Size: 93 6484
BsmBI CGTCTCn'nnnn_
Cuts at: 2048 3510 2048
Size: 1462 5115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2302 3619 3772 3896 4638 4723
Size: 399 249 263 1317 153 124 742 85
Cuts at: 4723 5794 1391
Size: 1071 2174
Fragments arranged by size:
2174 1317 1071 742 399 263 249 153
124 85
BspEI T'CCGG_A
Cuts at: 6240 6240
Size: 6577
BspHI T'CATG_A
Cuts at: 3607 4466 6196 3607
Size: 859 1730 3988
Fragments arranged by size:
3988 1730 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3932 844
Size: 3088 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4464 1394
Size: 429 2641 3507
Fragments arranged by size:
3507 2641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2330 2892 3415 5072 5246 65
Size: 1183 1082 562 523 1657 174 1396
Fragments arranged by size:
1657 1396 1183 1082 562 523 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5221 5408 1700
Size: 110 3411 187 2869
Fragments arranged by size:
3411 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6577
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6577
BssSI C'ACGA_G
Cuts at: 4637 5649 4637
Size: 1012 5565
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2759 794
Size: 1965 4612
BstBI TT'CG_AA
Cuts at: 3541 5381 3541
Size: 1840 4737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6577
BstYI r'GATC_y
Cuts at: 1972 2047 2204 2305 2369 3328 3907 4274 4659
Size: 75 157 101 64 959 579 367 385
Cuts at: 4659 4676 6128 6214 6226 6572 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 959 579 385 367 346 157
101 86 75 64 17 12
BstZ17I GTA'TAC
Cuts at: 3292 3292
Size: 6577
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5838
Fragments arranged by size:
5838 683 56
BtsI GCAGTG_nn'
Cuts at: 3798 4093 4887 4907 3798
Size: 295 794 20 5468
Fragments arranged by size:
5468 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6577
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6542
DraI TTT'AAA
Cuts at: 2728 2749 3336 3881 2728
Size: 21 587 545 5424
Fragments arranged by size:
5424 587 545 21
DraIII CAC_nnn'GTG
Cuts at: 3029 3029
Size: 6577
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5584 809
Size: 507 4268 1802
Fragments arranged by size:
4268 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4024 4913 6542
Size: 54 1425 50 275 18 2194 889 1629
Cuts at: 6542 8
Size: 43
Fragments arranged by size:
2194 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4024 1537
Size: 275 2212 4090
Fragments arranged by size:
4090 2212 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2455 2822 2958 3133 3192 3292 4507 1353
Size: 1102 367 136 175 59 100 1215 3423
Fragments arranged by size:
3423 1215 1102 367 175 136 100 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3258 5164 5538 5684 387
Size: 1064 138 1669 1906 374 146 1280
Fragments arranged by size:
1906 1669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2439 3090 3780 4640 5237 6024
Size: 393 564 243 651 690 860 597 787
Cuts at: 6024 6292 1239
Size: 268 1524
Fragments arranged by size:
1524 860 787 690 651 597 564 393
268 243
EcoO109I rG'GnC_Cy
Cuts at: 2089 2939 2089
Size: 850 5727
EcoRI G'AATT_C
Cuts at: 3886 4264 3886
Size: 378 6199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3008 3008
Size: 6577
FspI TGC'GCA
Cuts at: 5083 5083
Size: 6577
HaeII r_GCGC'y
Cuts at: 1678 2083 5724 1678
Size: 405 3641 2531
Fragments arranged by size:
3641 2531 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2193 2225 2555
Size: 32 135 32 473 32 116 32 330
Cuts at: 2555 2587 3360 3392 5218 5250 5292 5324 1373
Size: 32 773 32 1826 32 42 32 2626
Fragments arranged by size:
2626 1826 773 473 330 135 116 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2390 2530 3400 3921 4167 669
Size: 988 281 452 140 870 521 246 3079
Fragments arranged by size:
3079 988 870 521 452 281 246 140
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6577
HpaI GTT'AAC
Cuts at: 4167 4167
Size: 6577
KpnI G_GTAC'C
Cuts at: 3902 3902
Size: 6577
MfeI C'AATT_G
Cuts at: 3253 4176 3253
Size: 923 5654
MluI A'CGCG_T
Cuts at: 2633 6453 2633
Size: 3820 2757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5691 5875 1305
Size: 684 3702 184 2007
Fragments arranged by size:
3702 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4755
Fragments arranged by size:
4755 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3612 4535 4894 5053 518
Size: 648 2446 923 359 159 2042
Fragments arranged by size:
2446 2042 923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2820 3993 4670 5818 6063 1606
Size: 1214 1173 677 1148 245 2120
Fragments arranged by size:
2120 1214 1173 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6025
NdeI CA'TA_TG
Cuts at: 387 3739 387
Size: 3352 3225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6577
NotI GC'GGCC_GC
Cuts at: 4024 4024
Size: 6577
NspI r_CATG'y
Cuts at: 1132 1229 2696 3935 5480 1132
Size: 97 1467 1239 1545 2229
Fragments arranged by size:
2229 1545 1467 1239 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6577
PciI A'CATG_T
Cuts at: 2692 5476 2692
Size: 2784 3793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6577
PmeI GTTT'AAAC
Cuts at: 3881 3881
Size: 6577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4646 4678 6159 6191 4646
Size: 32 1481 32 5032
Fragments arranged by size:
5032 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 6577
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6577
PsiI TTA'TAA
Cuts at: 4147 4147
Size: 6577
PstI C_TGCA'G
Cuts at: 830 2201 3859 3929 830
Size: 1371 1658 70 3478
Fragments arranged by size:
3478 1658 1371 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6577
PvuII CAG'CTG
Cuts at: 2820 2820
Size: 6577
SalI G'TCGA_C
Cuts at: 1936 2388 3919 1936
Size: 452 1531 4594
Fragments arranged by size:
4594 1531 452
SapI GCTCTTCn'nnn_
Cuts at: 3133 3133
Size: 6577
SbfI CC_TGCA'GG
Cuts at: 3929 3929
Size: 6577
ScaI AGT'ACT
Cuts at: 2546 4825 6537 2546
Size: 2279 1712 2586
Fragments arranged by size:
2586 2279 1712
SfcI C'TryA_G
Cuts at: 826 1045 2197 2537 3118 3426 3855 3925 5060
Size: 219 1152 340 581 308 429 70 1135
Cuts at: 5060 5741 5932 826
Size: 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 581 429 340 308
219 191 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6577
SmaI CCC'GGG
Cuts at: 1872 3904 1872
Size: 2032 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 4681 5582 5844 820
Size: 197 926 954 1784 901 262 1553
Fragments arranged by size:
1784 1553 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4368 493
Size: 3875 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6577
SphI G_CATG'C
Cuts at: 1132 3935 1132
Size: 2803 3774
SspI AAT'ATT
Cuts at: 5 52 4501 5
Size: 47 4449 2081
Fragments arranged by size:
4449 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6577
StyI C'CwwG_G
Cuts at: 513 1065 3675 4016 513
Size: 552 2610 341 3074
Fragments arranged by size:
3074 2610 552 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2109 4604 4621 4774 4959 5404 325
Size: 1255 529 2495 17 153 185 445 1498
Fragments arranged by size:
2495 1498 1255 529 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 4823 6535
Size: 274 80 33 51 1443 567 2279 1712
Cuts at: 6535 96
Size: 138
Fragments arranged by size:
2279 1712 1443 567 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6537 6569 6537
Size: 32 6545
Tth111I GACn'n_nGTC
Cuts at: 1316 2059 1316
Size: 743 5834
XbaI T'CTAG_A
Cuts at: 3913 3913
Size: 6577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3259 3259
Size: 6577
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6577
XmaI C'CCGG_G
Cuts at: 1870 3902 1870
Size: 2032 4545
XmnI GAAnn'nnTTC
Cuts at: 3435 4706 3435
Size: 1271 5306
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4385 276
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI