Restriction map for
pFN21AB3037
(Circular) (Six-base) MAPSORT of: pFN21AB3037.seq Check: 3075 from: 1 to: 8155
pFN21AB3037 8155 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5965 278
Size: 53 83 186 936 4429 2468
Fragments arranged by size:
4429 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5498 1937
Size: 3561 4594
Acc65I G'GTAC_C
Cuts at: 5476 5476
Size: 8155
AclI AA'CG_TT
Cuts at: 1563 6282 6655 1563
Size: 4719 373 3063
Fragments arranged by size:
4719 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 2643 3945 4170 4454 4926 5150 6218
Size: 639 372 1302 225 284 472 224 1068
Cuts at: 6218 7602 7870 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1302 1068 639 472 372 284
268 225 224
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7958
AflIII A'CryG_T
Cuts at: 1203 7054 8031 1203
Size: 5851 977 1327
Fragments arranged by size:
5851 1327 977
AgeI A'CCGG_T
Cuts at: 3644 5265 6986 3644
Size: 1621 1721 4813
Fragments arranged by size:
4813 1721 1621
AhdI GACnn_n'nnGTC
Cuts at: 6884 6884
Size: 8155
AleI CACnn'nnGTG
Cuts at: 1166 2151 1166
Size: 985 7170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4646 1184
Size: 3462 4693
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 2582 2614 2504
Size: 32 46 32 8045
Fragments arranged by size:
8045 46 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3032 5898 7470 3032
Size: 2866 1572 3717
Fragments arranged by size:
3717 2866 1572
ApaLI G'TGCA_C
Cuts at: 6212 7368 6212
Size: 1156 6999
ApoI r'AATT_y
Cuts at: 1493 2381 2888 3127 3353 3476 3508 3873 4403
Size: 888 507 239 226 123 32 365 530
Cuts at: 4403 4523 4721 5375 5464 5690 5842 7951 1493
Size: 120 198 654 89 226 152 2109 1697
Fragments arranged by size:
2109 1697 888 654 530 507 365 239
226 226 198 152 123 120 89 32
AseI AT'TA_AT
Cuts at: 160 1032 6709 160
Size: 872 5677 1606
Fragments arranged by size:
5677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8155
AvaI C'yCGr_G
Cuts at: 1870 1943 5480 1870
Size: 73 3537 4545
Fragments arranged by size:
4545 3537 73
BamHI G'GATC_C
Cuts at: 5485 5852 5485
Size: 367 7788
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5476 6931 618
Size: 325 217 627 3689 1455 1842
Fragments arranged by size:
3689 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3772 5049 5253 5474 1391
Size: 2381 1277 204 221 4072
Fragments arranged by size:
4072 2381 1277 221 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2634 3459 3807 4331 4406 4917 928
Size: 971 735 825 348 524 75 511 4166
Fragments arranged by size:
4166 971 825 735 524 511 348 75
BbvCI CC'TCA_GC
Cuts at: 5277 5277
Size: 8155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6346 6380 1903
Size: 34 4409 34 3678
Fragments arranged by size:
4409 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2578 4477 6047 7257 2578
Size: 1899 1570 1210 3476
Fragments arranged by size:
3476 1899 1570 1210
BclI T'GATC_A
Cuts at: 1548 2934 3993 4741 1548
Size: 1386 1059 748 4962
Fragments arranged by size:
4962 1386 1059 748
BfrBI ATG'CAT
Cuts at: 2763 2763
Size: 8155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6766 136
Size: 107 122 71 631 605 5094 1525
Fragments arranged by size:
5094 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2203 3661 4580 5125 8150 2203
Size: 1458 919 545 3025 2208
Fragments arranged by size:
3025 2208 1458 919 545
BlpI GC'TnA_GC
Cuts at: 5572 5572
Size: 8155
Bme1580I G_kGCm'C
Cuts at: 1790 6216 7372 1790
Size: 4426 1156 2573
Fragments arranged by size:
4426 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2969 5269 5886
Size: 416 53 83 399 24 1552 2300 617
Cuts at: 5886 6844 442
Size: 958 1753
Fragments arranged by size:
2300 1753 1552 958 617 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3969 4029 4503 5015 5198 6815 1239
Size: 2730 60 474 512 183 1617 2579
Fragments arranged by size:
2730 2579 1617 512 474 183 60
Bpu10I CC'TnA_GC
Cuts at: 5247 5277 5247
Size: 30 8125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3686 3795 6280 7145 7443 3686
Size: 109 2485 865 298 4398
Fragments arranged by size:
4398 2485 865 298 109
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3483 3569 4502 6818 882
Size: 617 1984 86 933 2316 2219
Fragments arranged by size:
2316 2219 1984 933 617 86
BsaAI yAC'GTr
Cuts at: 493 1204 5946 493
Size: 711 4742 2702
Fragments arranged by size:
4742 2702 711
BsaBI GATnn'nnATC
Cuts at: 2202 4740 8149 2202
Size: 2538 3409 2208
Fragments arranged by size:
3409 2538 2208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5962 6344 6979 275
Size: 53 83 186 936 4429 382 635 1451
Fragments arranged by size:
4429 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3644 5265 6588 6986 7260 7407 7818 3644
Size: 1621 1323 398 274 147 411 3981
Fragments arranged by size:
3981 1621 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4959 4989 1178
Size: 30 165 30 3556 30 4344
Fragments arranged by size:
4344 3556 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5024 5294 1186
Size: 3838 270 4047
Fragments arranged by size:
4047 3838 270
BseYI C'CCAG_C
Cuts at: 7358 7854 7358
Size: 496 7659
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2181 4851 5158 1163
Size: 573 445 2670 307 4160
Fragments arranged by size:
4160 2670 573 445 307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5605 6366 7394 1540
Size: 275 186 3604 761 1028 2301
Fragments arranged by size:
3604 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6216 6301 7372 6216
Size: 85 1071 6999
Fragments arranged by size:
6999 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 4114 5666 5759 3830
Size: 284 1552 93 6226
Fragments arranged by size:
6226 1552 284 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4962 5282 4962
Size: 320 7835
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3772 5049 5253 5474 6216 6301 7372
Size: 399 1982 1277 204 221 742 85 1071
Cuts at: 7372 1391
Size: 2174
Fragments arranged by size:
2174 1982 1277 1071 742 399 221 204
85
BspEI T'CCGG_A
Cuts at: 7818 7818
Size: 8155
BspHI T'CATG_A
Cuts at: 3319 4177 4387 6044 7774 3319
Size: 858 210 1657 1730 3700
Fragments arranged by size:
3700 1730 1657 858 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 5368 5510 844
Size: 1270 3254 142 3489
Fragments arranged by size:
3489 3254 1270 142
BsrBI CCG'CTC
Cuts at: 1394 1823 6042 1394
Size: 429 4219 3507
Fragments arranged by size:
4219 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4353 6650 6824 65
Size: 1183 3105 2297 174 1396
Fragments arranged by size:
3105 2297 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 3644 5265 6799 6986 1700
Size: 110 257 1577 1621 1534 187 2869
Fragments arranged by size:
2869 1621 1577 1534 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 3100 96
Size: 3004 5151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8155
BssSI C'ACGA_G
Cuts at: 6215 7227 6215
Size: 1012 7143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8155
BstBI TT'CG_AA
Cuts at: 6959 6959
Size: 8155
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8155
BstXI CCAn_nnnn'nTGG
Cuts at: 2234 2234
Size: 8155
BstYI r'GATC_y
Cuts at: 1972 2203 2303 3661 4580 5125 5206 5485 5852
Size: 231 100 1358 919 545 81 279 367
Cuts at: 5852 6237 6254 7706 7792 7804 8150 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1358 919 545 385 367 346
279 231 100 86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 3447 3447
Size: 8155
BtgI C'CryG_G
Cuts at: 513 1065 2004 4133 4545 5034 513
Size: 552 939 2129 412 489 3634
Fragments arranged by size:
3634 2129 939 552 489 412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7416
Fragments arranged by size:
7416 683 56
BtsI GCAGTG_nn'
Cuts at: 5021 5671 6465 6485 5021
Size: 650 794 20 6691
Fragments arranged by size:
6691 794 650 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8120
DraI TTT'AAA
Cuts at: 2733 3624 5459 2733
Size: 891 1835 5429
Fragments arranged by size:
5429 1835 891
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 7162 809
Size: 507 3029 2817 1802
Fragments arranged by size:
3029 2817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2491 5602 6491
Size: 54 1425 50 275 18 661 3111 889
Cuts at: 6491 8120 8
Size: 1629 43
Fragments arranged by size:
3111 1629 1425 889 661 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5602 1537
Size: 275 3790 4090
Fragments arranged by size:
4090 3790 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3255 3477 3519 3691 3753 4650 5126 6085
Size: 1902 222 42 172 62 897 476 959
Cuts at: 6085 1353
Size: 3423
Fragments arranged by size:
3423 1902 959 897 476 222 172 62
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6742 7116 7262 387
Size: 1064 138 5153 374 146 1280
Fragments arranged by size:
5153 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2643 3945 3969 4029 4170 4454
Size: 393 639 372 1302 24 60 141 284
Cuts at: 4454 4503 4926 5015 5150 5198 6218 6815 7602
Size: 49 423 89 135 48 1020 597 787
Cuts at: 7602 7870 1239
Size: 268 1524
Fragments arranged by size:
1524 1302 1020 787 639 597 423 393
372 284 268 141 135 89 60 49
48 24
EcoRI G'AATT_C
Cuts at: 2888 4523 5464 5842 2888
Size: 1635 941 378 5201
Fragments arranged by size:
5201 1635 941 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 8155
FspI TGC'GCA
Cuts at: 6661 6661
Size: 8155
HaeII r_GCGC'y
Cuts at: 1678 7302 1678
Size: 5624 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2337 2369 3446
Size: 32 135 32 623 32 110 32 1077
Cuts at: 3446 3478 3539 3571 3659 3691 4382 4414 4673
Size: 32 61 32 88 32 691 32 259
Cuts at: 4673 4705 6796 6828 6870 6902 1373
Size: 32 2091 32 42 32 2626
Fragments arranged by size:
2626 2091 1077 691 623 259 135 110
88 61 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 4576 5499 5745 669
Size: 988 281 1704 934 923 246 3079
Fragments arranged by size:
3079 1704 988 934 923 281 246
HindIII A'AGCT_T
Cuts at: 748 5432 748
Size: 4684 3471
HpaI GTT'AAC
Cuts at: 5745 5745
Size: 8155
KpnI G_GTAC'C
Cuts at: 5480 5480
Size: 8155
MfeI C'AATT_G
Cuts at: 5754 5754
Size: 8155
MluI A'CGCG_T
Cuts at: 8031 8031
Size: 8155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7269 7453 1305
Size: 5964 184 2007
Fragments arranged by size:
5964 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2493 10
Size: 54 1425 343 661 5672
Fragments arranged by size:
5672 1425 661 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 2180 2232 2693 3395 4358 4389
Size: 648 985 29 52 461 702 963 31
Cuts at: 4389 4392 4704 4958 6113 6472 6631 518
Size: 3 312 254 1155 359 159 2042
Fragments arranged by size:
2042 1155 985 963 702 648 461 359
312 254 159 52 31 29 3
MspA1I CmG'CkG
Cuts at: 1606 4950 5099 5276 5571 6248 7396 7641 1606
Size: 3344 149 177 295 677 1148 245 2120
Fragments arranged by size:
3344 2120 1148 677 295 245 177 149
NcoI C'CATG_G
Cuts at: 513 1065 2004 5034 513
Size: 552 939 3030 3634
Fragments arranged by size:
3634 3030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8155
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8155
NotI GC'GGCC_GC
Cuts at: 5602 5602
Size: 8155
NsiI A_TGCA'T
Cuts at: 2765 2765
Size: 8155
NspI r_CATG'y
Cuts at: 1132 1229 3368 5513 7058 1132
Size: 97 2139 2145 1545 2229
Fragments arranged by size:
2229 2145 2139 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2289 1348
Size: 941 7214
PciI A'CATG_T
Cuts at: 7054 7054
Size: 8155
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2525 2981 1108
Size: 1417 456 6282
Fragments arranged by size:
6282 1417 456
PfoI T'CCnGG_A
Cuts at: 3786 5325 3786
Size: 1539 6616
PmeI GTTT'AAAC
Cuts at: 5459 5459
Size: 8155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4928 4960 6224 6256 7737 7769 4928
Size: 32 1264 32 1481 32 5314
Fragments arranged by size:
5314 1481 1264 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8155
PsiI TTA'TAA
Cuts at: 2499 5725 2499
Size: 3226 4929
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2070 2102 2070
Size: 32 8123
PstI C_TGCA'G
Cuts at: 830 4315 4436 5507 830
Size: 3485 121 1071 3478
Fragments arranged by size:
3485 3478 1071 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8155
PvuII CAG'CTG
Cuts at: 5099 5099
Size: 8155
SalI G'TCGA_C
Cuts at: 1936 5497 1936
Size: 3561 4594
SbfI CC_TGCA'GG
Cuts at: 5507 5507
Size: 8155
ScaI AGT'ACT
Cuts at: 2035 5000 6403 8115 2035
Size: 2965 1403 1712 2075
Fragments arranged by size:
2965 2075 1712 1403
SexAI A'CCwGG_T
Cuts at: 2852 2852
Size: 8155
SfcI C'TryA_G
Cuts at: 826 1045 2047 3878 3922 4168 4311 4432 5503
Size: 219 1002 1831 44 246 143 121 1071
Cuts at: 5503 6638 7319 7510 826
Size: 1135 681 191 1471
Fragments arranged by size:
1831 1471 1135 1071 1002 681 246 219
191 143 121 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8155
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8155
SmaI CCC'GGG
Cuts at: 1872 5482 1872
Size: 3610 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3665 3810 6259 7160 7422 820
Size: 197 926 1722 145 2449 901 262 1553
Fragments arranged by size:
2449 1722 1553 926 901 262 197 145
SnaBI TAC'GTA
Cuts at: 493 5946 493
Size: 5453 2702
SpeI A'CTAG_T
Cuts at: 152 3817 152
Size: 3665 4490
SphI G_CATG'C
Cuts at: 1132 5513 1132
Size: 4381 3774
SspI AAT'ATT
Cuts at: 5 52 4563 5400 6079 5
Size: 47 4511 837 679 2081
Fragments arranged by size:
4511 2081 837 679 47
StuI AGG'CCT
Cuts at: 1517 2273 1517
Size: 756 7399
StyI C'CwwG_G
Cuts at: 513 1065 2004 5034 5594 513
Size: 552 939 3030 560 3074
Fragments arranged by size:
3074 3030 939 560 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3706 6182 6199 6352 6537 6982 325
Size: 1255 2126 2476 17 153 185 445 1498
Fragments arranged by size:
2476 2126 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2033 3100 4975
Size: 274 80 33 51 1443 56 1067 1875
Cuts at: 4975 4998 6401 8113 96
Size: 23 1403 1712 138
Fragments arranged by size:
1875 1712 1443 1403 1067 274 138 80
56 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8115 8147 8115
Size: 32 8123
Tth111I GACn'n_nGTC
Cuts at: 1316 3315 1316
Size: 1999 6156
XbaI T'CTAG_A
Cuts at: 2917 4441 5491 2917
Size: 1524 1050 5581
Fragments arranged by size:
5581 1524 1050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4990 4990
Size: 8155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8155
XmaI C'CCGG_G
Cuts at: 1870 5480 1870
Size: 3610 4545
XmnI GAAnn'nnTTC
Cuts at: 6284 6284
Size: 8155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5963 276
Size: 53 83 186 936 4429 2468
Fragments arranged by size:
4429 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI