Restriction map for
pFN21AB3035
(Circular) (Six-base) MAPSORT of: pFN21AB3035.seq Check: 9296 from: 1 to: 6568
pFN21AB3035 6568 bp
With 182 enzymes: SgfI *
April 28, 2014 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2737 3162 3436 2737
Size: 425 274 5869
Fragments arranged by size:
5869 425 274
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 1937
Size: 1974 4594
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2876 3557 4631 6015 6283 1632
Size: 404 840 681 1074 1384 268 1917
Fragments arranged by size:
1917 1384 1074 840 681 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 2777 5467 6444 1203
Size: 1574 2690 977 1327
Fragments arranged by size:
2690 1574 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3170 1184
Size: 1986 4582
AlwNI CAG_nnn'CTG
Cuts at: 2851 2854 4311 5883 2851
Size: 3 1457 1572 3536
Fragments arranged by size:
3536 1572 1457 3
ApaI G_GGCC'C
Cuts at: 3116 3184 3409 3116
Size: 68 225 6275
Fragments arranged by size:
6275 225 68
ApaLI G'TGCA_C
Cuts at: 3423 4625 5781 3423
Size: 1202 1156 4210
Fragments arranged by size:
4210 1202 1156
ApoI r'AATT_y
Cuts at: 1493 3877 4103 4255 6364 1493
Size: 2384 226 152 2109 1697
Fragments arranged by size:
2384 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 3109 3175 3402 3714 3893 1870
Size: 73 1166 66 227 312 179 4545
Fragments arranged by size:
4545 1166 312 227 179 73 66
BamHI G'GATC_C
Cuts at: 3898 4265 3898
Size: 367 6201
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 2579 3889 5344 618
Size: 325 217 627 734 58 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 734 627 325 217 58
BanII G_rGCy'C
Cuts at: 1391 3049 3116 3174 3184 3409 3454 3695 3887
Size: 1658 67 58 10 225 45 241 192
Cuts at: 3887 1391
Size: 4072
Fragments arranged by size:
4072 1658 241 225 192 67 58 45
10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5597
BbvCI CC'TCA_GC
Cuts at: 3137 3390 3137
Size: 253 6315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3575 3609 3719 3753 4759 4793 1903
Size: 34 1638 34 110 34 1006 34 3678
Fragments arranged by size:
3678 1638 1006 110 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4460 5670 4460
Size: 1210 5358
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2111 2937
Size: 107 122 71 631 605 337 102 826
Cuts at: 2937 3163 3225 3316 5179 136
Size: 226 62 91 1863 1525
Fragments arranged by size:
1863 1525 826 631 605 337 226 122
107 102 91 71 62
BglII A'GATC_T
Cuts at: 6563 6563
Size: 6568
BlpI GC'TnA_GC
Cuts at: 2673 3048 3985 2673
Size: 375 937 5256
Fragments arranged by size:
5256 937 375
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2190 2526 3081 3116 3184 3307 3409
Size: 317 83 336 555 35 68 123 102
Cuts at: 3409 3427 4629 5785 1790
Size: 18 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 555 336 317 123 102
83 68 35 18
BmgBI CAC'GTC
Cuts at: 1318 3507 3513 1318
Size: 2189 6 4373
Fragments arranged by size:
4373 2189 6
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4299 5257 442
Size: 416 53 83 399 24 2882 958 1753
Fragments arranged by size:
2882 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2478 2726 2911 3127 3188 5228 1239
Size: 1051 188 248 185 216 61 2040 2579
Fragments arranged by size:
2579 2040 1051 248 216 188 185 61
Bpu10I CC'TnA_GC
Cuts at: 2766 3137 3390 2766
Size: 371 253 5944
Fragments arranged by size:
5944 371 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4693 5558 5856 4693
Size: 865 298 5405
Fragments arranged by size:
5405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2907 5231 882
Size: 617 1408 2324 2219
Fragments arranged by size:
2324 2219 1408 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 2778 4359 493
Size: 711 882 692 1581 2702
Fragments arranged by size:
2702 1581 882 711 692
BsaBI GATnn'nnATC
Cuts at: 6562 6562
Size: 6568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3521 3666 4375 4757
Size: 53 83 186 936 1988 145 709 382
Cuts at: 4757 5392 275
Size: 635 1451
Fragments arranged by size:
1988 1451 936 709 635 382 186 145
83 53
BsaWI w'CCGG_w
Cuts at: 2871 5001 5399 5673 5820 6231 2871
Size: 2130 398 274 147 411 3208
Fragments arranged by size:
3208 2130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2422 2452 1178
Size: 30 165 30 1019 30 5294
Fragments arranged by size:
5294 1019 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 2435 2729 3544 3713 1186
Size: 1246 3 294 815 169 4041
Fragments arranged by size:
4041 1246 815 294 169 3
BseYI C'CCAG_C
Cuts at: 2202 3070 3259 3306 3311 3408 3454 3657 3669
Size: 868 189 47 5 97 46 203 12
Cuts at: 3669 5771 6267 2202
Size: 2102 496 2503
Fragments arranged by size:
2503 2102 868 496 203 189 97 47
46 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 2831 3605 3782 1163
Size: 573 888 207 774 177 3949
Fragments arranged by size:
3949 888 774 573 207 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2260 3229 4018 4779 5807 1540
Size: 275 186 259 969 789 761 1028 2301
Fragments arranged by size:
2301 1028 969 789 761 275 259 186
BsiHKAI G_wGCw'C
Cuts at: 2868 2897 3049 3243 3427 4629 4714 5785 2868
Size: 29 152 194 184 1202 85 1071 3651
Fragments arranged by size:
3651 1202 1071 194 184 152 85 29
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 3512 2417
Size: 1095 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2190 2526 2868 2897 3049 3081
Size: 399 317 83 336 342 29 152 32
Cuts at: 3081 3116 3174 3184 3243 3307 3409 3427 3454
Size: 35 58 10 59 64 102 18 27
Cuts at: 3454 3695 3887 4629 4714 5785 1391
Size: 241 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 336 317 241
192 152 102 85 83 64 59 58
35 32 29 27 18 10
BspEI T'CCGG_A
Cuts at: 2871 6231 2871
Size: 3360 3208
BspHI T'CATG_A
Cuts at: 4457 6187 4457
Size: 1730 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2737 3162 3436 3491 3627 3804 3923 844
Size: 1893 425 274 55 136 177 119 3489
Fragments arranged by size:
3489 1893 425 274 177 136 119 55
BsrBI CCG'CTC
Cuts at: 1394 1823 4455 1394
Size: 429 2632 3507
Fragments arranged by size:
3507 2632 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3214 5063 5237 65
Size: 1183 1966 1849 174 1396
Fragments arranged by size:
1966 1849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5212 5399 1700
Size: 110 3402 187 2869
Fragments arranged by size:
3402 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2780 2799 96
Size: 2684 19 3865
Fragments arranged by size:
3865 2684 19
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 4628 5640 4628
Size: 1012 5556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3727 794
Size: 2933 3635
BstBI TT'CG_AA
Cuts at: 5372 5372
Size: 6568
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6568
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2554
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 3898 4265 4650 4667 6119 6205 6217 6563
Size: 1926 367 385 17 1452 86 12 346
Cuts at: 6563 1972
Size: 1977
Fragments arranged by size:
1977 1926 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2108 2108
Size: 6568
BtgI C'CryG_G
Cuts at: 513 1065 2004 3161 513
Size: 552 939 1157 3920
Fragments arranged by size:
3920 1157 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3814 487
Size: 683 56 2588 3241
Fragments arranged by size:
3241 2588 683 56
BtsI GCAGTG_nn'
Cuts at: 2783 4084 4878 4898 2783
Size: 1301 794 20 4453
Fragments arranged by size:
4453 1301 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DraIII CAC_nnn'GTG
Cuts at: 2364 2364
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2257 2751 3085
Size: 54 1425 50 275 18 427 494 334
Cuts at: 3085 3195 3226 4015 4904 6533 8
Size: 110 31 789 889 1629 43
Fragments arranged by size:
1629 1425 889 789 494 427 334 275
110 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2257 3226 4015 1537
Size: 275 445 969 789 4090
Fragments arranged by size:
4090 969 789 445 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 3562 4498 1353
Size: 1214 995 936 3423
Fragments arranged by size:
3423 1214 995 936
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2429 2729 5155 5529 5675 387
Size: 1064 138 840 300 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 840 374 300 146 138
EcoICRI GAG'CTC
Cuts at: 3047 3047
Size: 6568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2290 2478 2726 2876 2911 3127
Size: 393 404 254 188 248 150 35 216
Cuts at: 3127 3188 3557 4631 5228 6015 6283 1239
Size: 61 369 1074 597 787 268 1524
Fragments arranged by size:
1524 1074 787 597 404 393 369 268
254 248 216 188 150 61 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2744 2708
Size: 36 6532
EcoO109I rG'GnC_Cy
Cuts at: 2112 2619 3180 2112
Size: 507 561 5500
Fragments arranged by size:
5500 561 507
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
EcoRV GAT'ATC
Cuts at: 3027 3027
Size: 6568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 6568
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 2015 2953 3571 5715 1678
Size: 337 938 618 2144 2531
Fragments arranged by size:
2531 2144 938 618 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5209 5241 5283 5315 1373
Size: 32 135 32 3637 32 42 32 2626
Fragments arranged by size:
3637 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3061 3912 4158 669
Size: 988 281 1123 851 246 3079
Fragments arranged by size:
3079 1123 988 851 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6568
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 3495 5682 5866 1305
Size: 969 1221 2187 184 2007
Fragments arranged by size:
2187 2007 1221 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 10
Size: 54 1425 343 921 3825
Fragments arranged by size:
3825 1425 921 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4526 4885 5044 518
Size: 648 3360 359 159 2042
Fragments arranged by size:
3360 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2604 2851 3163 3225 3786 3847 3853 3984
Size: 998 247 312 62 561 61 6 131
Cuts at: 3984 4661 5809 6054 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 998 677 561 312 247 245
131 62 61 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5077
Fragments arranged by size:
5077 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 2800 3926 5471 1132
Size: 97 1571 1126 1545 2229
Fragments arranged by size:
2229 1571 1545 1126 97
PasI CC'CwG_GG
Cuts at: 1348 2272 3688 1348
Size: 924 1416 4228
Fragments arranged by size:
4228 1416 924
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2749 1108
Size: 1641 4927
PfoI T'CCnGG_A
Cuts at: 2528 2528
Size: 6568
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PmlI CAC'GTG
Cuts at: 2086 2778 2086
Size: 692 5876
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PspOMI G'GGCC_C
Cuts at: 3112 3180 3405 3112
Size: 68 225 6275
Fragments arranged by size:
6275 225 68
PspXI vC'TCGA_Gb
Cuts at: 3714 3714
Size: 6568
PstI C_TGCA'G
Cuts at: 830 2694 2790 2982 3488 3858 3920 830
Size: 1864 96 192 506 370 62 3478
Fragments arranged by size:
3478 1864 506 370 192 96 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2851 3853 2851
Size: 1002 5566
SacI G_AGCT'C
Cuts at: 3049 3049
Size: 6568
SacII CC_GC'GG
Cuts at: 3164 3164
Size: 6568
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SfcI C'TryA_G
Cuts at: 826 1045 2690 2786 2978 3484 3766 3854 3916
Size: 219 1645 96 192 506 282 88 62
Cuts at: 3916 5051 5732 5923 826
Size: 1135 681 191 1471
Fragments arranged by size:
1645 1471 1135 681 506 282 219 192
191 96 88 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 3225 3225
Size: 6568
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3111 3404 3895 1872
Size: 1239 293 491 4545
Fragments arranged by size:
4545 1239 491 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3714 4672 5573 5835 820
Size: 197 926 1771 958 901 262 1553
Fragments arranged by size:
1771 1553 958 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 4492 5
Size: 47 4440 2081
Fragments arranged by size:
4440 2081 47
StuI AGG'CCT
Cuts at: 1517 3095 1517
Size: 1578 4990
StyI C'CwwG_G
Cuts at: 513 1065 2004 2182 2908 4007 513
Size: 552 939 178 726 1099 3074
Fragments arranged by size:
3074 1099 939 726 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2646 3449 3671 4595 4612 4765 4950
Size: 1255 1066 803 222 924 17 153 185
Cuts at: 4950 5395 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1066 924 803 445 222 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2780 2799 3600
Size: 274 80 33 51 1443 803 19 801
Cuts at: 3600 4814 6526 96
Size: 1214 1712 138
Fragments arranged by size:
1712 1443 1214 803 801 274 138 80
51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2422 2454 2563 2595 2762 2794 6528 6560 2422
Size: 32 109 32 167 32 3734 32 2430
Fragments arranged by size:
3734 2430 167 109 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 3714 1943
Size: 1771 4797
XmaI C'CCGG_G
Cuts at: 1870 3109 3402 3893 1870
Size: 1239 293 491 4545
Fragments arranged by size:
4545 1239 491 293
XmnI GAAnn'nnTTC
Cuts at: 3587 4697 3587
Size: 1110 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PsrI RsrII SanDI SapI
SexAI SfoI SrfI SwaI XcmI