Restriction map for
pFN21AB3033
(Circular) (Six-base) MAPSORT of: pFN21AB3033.seq Check: 1013 from: 1 to: 6394
pFN21AB3033 6394 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2884 2884
Size: 6394
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2231 3471 3632 4204
Size: 53 83 186 936 695 1240 161 572
Cuts at: 4204 278
Size: 2468
Fragments arranged by size:
2468 1240 936 695 572 186 161 83
53
AccI GT'mk_AC
Cuts at: 1937 2739 3375 3737 1937
Size: 802 636 362 4594
Fragments arranged by size:
4594 802 636 362
Acc65I G'GTAC_C
Cuts at: 3715 3715
Size: 6394
AclI AA'CG_TT
Cuts at: 1563 4521 4894 1563
Size: 2958 373 3063
Fragments arranged by size:
3063 2958 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2195 2378 2411 4457 5841 6109 1632
Size: 482 81 183 33 2046 1384 268 1917
Fragments arranged by size:
2046 1917 1384 482 268 183 81 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6197
AflIII A'CryG_T
Cuts at: 1203 3681 5293 6270 1203
Size: 2478 1612 977 1327
Fragments arranged by size:
2478 1612 1327 977
AgeI A'CCGG_T
Cuts at: 2913 5225 2913
Size: 2312 4082
AhdI GACnn_n'nnGTC
Cuts at: 5123 5123
Size: 6394
AleI CACnn'nnGTG
Cuts at: 1166 2841 3488 1166
Size: 1675 647 4072
Fragments arranged by size:
4072 1675 647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6394
AlwNI CAG_nnn'CTG
Cuts at: 2908 4137 5709 2908
Size: 1229 1572 3593
Fragments arranged by size:
3593 1572 1229
ApaI G_GGCC'C
Cuts at: 2380 2805 3574 3608 2380
Size: 425 769 34 5166
Fragments arranged by size:
5166 769 425 34
ApaLI G'TGCA_C
Cuts at: 2972 4451 5607 2972
Size: 1479 1156 3759
Fragments arranged by size:
3759 1479 1156
ApoI r'AATT_y
Cuts at: 1493 3703 3929 4081 6190 1493
Size: 2210 226 152 2109 1697
Fragments arranged by size:
2210 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 2572 3573 3719 1870
Size: 73 629 1001 146 4545
Fragments arranged by size:
4545 1001 629 146 73
BamHI G'GATC_C
Cuts at: 3724 4091 3724
Size: 367 6027
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2668 3715 5170 618
Size: 325 217 627 230 651 1047 1455 1842
Fragments arranged by size:
1842 1455 1047 651 627 325 230 217
BanII G_rGCy'C
Cuts at: 1391 2112 2380 2805 3297 3330 3574 3608 3713
Size: 721 268 425 492 33 244 34 105
Cuts at: 3713 1391
Size: 4072
Fragments arranged by size:
4072 721 492 425 268 244 105 34
33
BbeI G_GCGC'C
Cuts at: 2021 2021
Size: 6394
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5423
BbvCI CC'TCA_GC
Cuts at: 3494 3494
Size: 6394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4585 4619 1903
Size: 34 50 34 2564 34 3678
Fragments arranged by size:
3678 2564 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4286 5496 4286
Size: 1210 5184
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6394
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 2723 5005
Size: 107 122 71 631 605 652 399 2282
Cuts at: 5005 136
Size: 1525
Fragments arranged by size:
2282 1525 652 631 605 399 122 107
71
BglII A'GATC_T
Cuts at: 2382 3345 6389 2382
Size: 963 3044 2387
Fragments arranged by size:
3044 2387 963
BlpI GC'TnA_GC
Cuts at: 3019 3811 3019
Size: 792 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2380 2571 2671 2805 2871 2976 3574 3608
Size: 590 191 100 134 66 105 598 34
Cuts at: 3608 4455 5611 1790
Size: 847 1156 2573
Fragments arranged by size:
2573 1156 847 598 590 191 134 105
100 66 34
BmgBI CAC'GTC
Cuts at: 1318 2236 2989 1318
Size: 918 753 4723
Fragments arranged by size:
4723 918 753
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 4125 5083
Size: 416 53 83 399 24 1283 1425 958
Cuts at: 5083 442
Size: 1753
Fragments arranged by size:
1753 1425 1283 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2564 2624 2961 3131 3236 3694 5054 1239
Size: 1325 60 337 170 105 458 1360 2579
Fragments arranged by size:
2579 1360 1325 458 337 170 105 60
Bpu10I CC'TnA_GC
Cuts at: 2139 3494 2139
Size: 1355 5039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4519 5384 5682 4519
Size: 865 298 5231
Fragments arranged by size:
5231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5057 882
Size: 617 3558 2219
Fragments arranged by size:
3558 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3070 4185 493
Size: 711 1866 1115 2702
Fragments arranged by size:
2702 1866 1115 711
BsaBI GATnn'nnATC
Cuts at: 6388 6388
Size: 6394
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2228 3468 3629
Size: 53 83 186 936 485 210 1240 161
Cuts at: 3629 4201 4583 5218 275
Size: 572 382 635 1451
Fragments arranged by size:
1451 1240 936 635 572 485 382 210
186 161 83 53
BsaWI w'CCGG_w
Cuts at: 2514 2913 4827 5225 5499 5646 6057 2514
Size: 399 1914 398 274 147 411 2851
Fragments arranged by size:
2851 1914 411 399 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 3212 3242 1178
Size: 30 165 30 669 30 1110 30 4330
Fragments arranged by size:
4330 1110 669 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 2231 3128 3220 3356 1186
Size: 891 154 897 92 136 4224
Fragments arranged by size:
4224 897 891 154 136 92
BseYI C'CCAG_C
Cuts at: 3236 5597 6093 3236
Size: 2361 496 3537
Fragments arranged by size:
3537 2361 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 3494 1163
Size: 573 522 1236 4063
Fragments arranged by size:
4063 1236 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2985 3844 4605 5633 1540
Size: 275 186 984 859 761 1028 2301
Fragments arranged by size:
2301 1028 984 859 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2579 2838 2976 3059 3120 3222 4455 4540 5611
Size: 259 138 83 61 102 1233 85 1071
Cuts at: 5611 2579
Size: 3362
Fragments arranged by size:
3362 1233 1071 259 138 102 85 83
61
BsmI GAATG_Cn'
Cuts at: 3505 3905 3998 3505
Size: 400 93 5901
Fragments arranged by size:
5901 400 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3068 3392 3068
Size: 324 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2380 2571 2579 2671 2805 2838
Size: 399 322 268 191 8 92 134 33
Cuts at: 2838 2871 2976 3059 3120 3222 3297 3330 3574
Size: 33 105 83 61 102 75 33 244
Cuts at: 3574 3608 3713 4455 4540 5611 1391
Size: 34 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 322 268 244 191
134 105 105 102 92 85 83 75
61 34 33 33 33 8
BspEI T'CCGG_A
Cuts at: 6057 6057
Size: 6394
BspHI T'CATG_A
Cuts at: 3333 4283 6013 3333
Size: 950 1730 3714
Fragments arranged by size:
3714 1730 950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2884 3628 3682 3749 844
Size: 2040 744 54 67 3489
Fragments arranged by size:
3489 2040 744 67 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2979 3006 3018 4281 1394
Size: 429 1156 27 12 1263 3507
Fragments arranged by size:
3507 1263 1156 429 27 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4889 5063 65
Size: 1183 3641 174 1396
Fragments arranged by size:
3641 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2063 2671 2913 3506 5038 5225
Size: 110 204 49 608 242 593 1532 187
Cuts at: 5225 1700
Size: 2869
Fragments arranged by size:
2869 1532 608 593 242 204 187 110
49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6394
BssSI C'ACGA_G
Cuts at: 4454 5466 4454
Size: 1012 5382
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6394
BstBI TT'CG_AA
Cuts at: 5198 5198
Size: 6394
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6394
BstXI CCAn_nnnn'nTGG
Cuts at: 2446 2446
Size: 6394
BstYI r'GATC_y
Cuts at: 1972 2382 2430 3345 3724 4091 4476 4493 5945
Size: 410 48 915 379 367 385 17 1452
Cuts at: 5945 6031 6043 6389 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 915 410 385 379 367 346
86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3321 3321
Size: 6394
BtgI C'CryG_G
Cuts at: 513 1065 2263 2445 3276 513
Size: 552 1198 182 831 3631
Fragments arranged by size:
3631 1198 831 552 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5655
Fragments arranged by size:
5655 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 2712 2869 3180 3453 3910 4704 4724 2640
Size: 72 157 311 273 457 794 20 4310
Fragments arranged by size:
4310 794 457 311 273 157 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6359
DraI TTT'AAA
Cuts at: 3698 3698
Size: 6394
DraIII CAC_nnn'GTG
Cuts at: 2971 2971
Size: 6394
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5401 809
Size: 507 4085 1802
Fragments arranged by size:
4085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2277 2619
Size: 54 1425 50 275 18 270 177 342
Cuts at: 2619 2724 2982 3037 3086 3252 3841 4730 6359
Size: 105 258 55 49 166 589 889 1629
Cuts at: 6359 8
Size: 43
Fragments arranged by size:
1629 1425 889 589 342 275 270 258
177 166 105 55 54 50 49 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2982 3841 1537
Size: 275 1170 859 4090
Fragments arranged by size:
4090 1170 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4324 1353
Size: 2971 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2018 2967 4981 5355 5501 387
Size: 1064 138 429 949 2014 374 146 1280
Fragments arranged by size:
2014 1280 1064 949 429 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2195 2378 2411 2564 2624 2961
Size: 393 482 81 183 33 153 60 337
Cuts at: 2961 3131 3236 3694 4457 5054 5841 6109 1239
Size: 170 105 458 763 597 787 268 1524
Fragments arranged by size:
1524 787 763 597 482 458 393 337
268 183 170 153 105 81 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2156 2753 2156
Size: 597 5797
EcoO109I rG'GnC_Cy
Cuts at: 2170 2353 2802 3009 3159 3316 3570 3604 2170
Size: 183 449 207 150 157 254 34 4960
Fragments arranged by size:
4960 449 254 207 183 157 150 34
EcoRI G'AATT_C
Cuts at: 3703 4081 3703
Size: 378 6016
FseI GG_CCGG'CC
Cuts at: 2067 2067
Size: 6394
FspI TGC'GCA
Cuts at: 4900 4900
Size: 6394
HaeII r_GCGC'y
Cuts at: 1678 2021 5541 1678
Size: 343 3520 2531
Fragments arranged by size:
3520 2531 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3173 3205 5035 5067 5109
Size: 32 135 32 1601 32 1830 32 42
Cuts at: 5109 5141 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 1601 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3415 3738 3984 669
Size: 988 281 1477 323 246 3079
Fragments arranged by size:
3079 1477 988 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6394
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KasI G'GCGC_C
Cuts at: 2017 2017
Size: 6394
KpnI G_GTAC'C
Cuts at: 3719 3719
Size: 6394
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 3681 6270 3681
Size: 2589 3805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 3104 5508 5692 1305
Size: 906 893 2404 184 2007
Fragments arranged by size:
2404 2007 906 893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 2279 2726 3039 10
Size: 54 1425 343 270 177 447 313 3365
Fragments arranged by size:
3365 1425 447 343 313 270 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 2717 2841 3488 4352 4711 4870
Size: 648 1398 153 124 647 864 359 159
Cuts at: 4870 518
Size: 2042
Fragments arranged by size:
2042 1398 864 648 647 359 159 153
124
MspA1I CmG'CkG
Cuts at: 1606 2495 2795 3409 3810 4487 5635 5880 1606
Size: 889 300 614 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 889 677 614 401 300 245
NaeI GCC'GGC
Cuts at: 2016 2065 3508 2016
Size: 49 1443 4902
Fragments arranged by size:
4902 1443 49
NarI GG'CG_CC
Cuts at: 2018 2018
Size: 6394
NcoI C'CATG_G
Cuts at: 513 1065 2263 2445 513
Size: 552 1198 182 4462
Fragments arranged by size:
4462 1198 552 182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6394
NgoMIV G'CCGG_C
Cuts at: 2014 2063 3506 2014
Size: 49 1443 4902
Fragments arranged by size:
4902 1443 49
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 2407 3691 3752 5297 1132
Size: 97 1178 1284 61 1545 2229
Fragments arranged by size:
2229 1545 1284 1178 97 61
PasI CC'CwG_GG
Cuts at: 1348 3664 1348
Size: 2316 4078
PciI A'CATG_T
Cuts at: 5293 5293
Size: 6394
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2798 2908 1108
Size: 1690 110 4594
Fragments arranged by size:
4594 1690 110
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4463 4495 5976 6008 4463
Size: 32 1481 32 4849
Fragments arranged by size:
4849 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2353 3009 3159 3316 2170
Size: 183 656 150 157 5248
Fragments arranged by size:
5248 656 183 157 150
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6394
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PspOMI G'GGCC_C
Cuts at: 2376 2801 3570 3604 2376
Size: 425 769 34 5166
Fragments arranged by size:
5166 769 425 34
PstI C_TGCA'G
Cuts at: 830 2494 3148 3175 3408 3746 830
Size: 1664 654 27 233 338 3478
Fragments arranged by size:
3478 1664 654 338 233 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
PvuII CAG'CTG
Cuts at: 2795 3409 2795
Size: 614 5780
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SanDI GG'GwC_CC
Cuts at: 3009 3009
Size: 6394
SbfI CC_TGCA'GG
Cuts at: 3746 3746
Size: 6394
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SfcI C'TryA_G
Cuts at: 826 1045 2490 3144 3171 3404 3742 4877 5558
Size: 219 1445 654 27 233 338 1135 681
Cuts at: 5558 5749 826
Size: 191 1471
Fragments arranged by size:
1471 1445 1135 681 654 338 233 219
191 27
SfoI GGC'GCC
Cuts at: 2019 2019
Size: 6394
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 3721 1872
Size: 1849 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4498 5399 5661 820
Size: 197 926 2555 901 262 1553
Fragments arranged by size:
2555 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4185 493
Size: 3692 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6394
SphI G_CATG'C
Cuts at: 1132 3752 1132
Size: 2620 3774
SspI AAT'ATT
Cuts at: 5 52 4318 5
Size: 47 4266 2081
Fragments arranged by size:
4266 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 1517
Size: 645 5749
StyI C'CwwG_G
Cuts at: 513 1065 2263 2445 3833 513
Size: 552 1198 182 1388 3074
Fragments arranged by size:
3074 1388 1198 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3250 4421 4438 4591 4776 5221 325
Size: 1255 1670 1171 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1255 1171 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3358 3390 4640
Size: 274 80 33 51 1443 1381 32 1250
Cuts at: 4640 6352 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1381 1250 274 138 80 51
33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2259 2291 2692 2724 3212 3244 3600
Size: 32 155 32 401 32 488 32 356
Cuts at: 3600 3632 6354 6386 2072
Size: 32 2722 32 2080
Fragments arranged by size:
2722 2080 488 401 356 155 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2819 1316
Size: 1503 4891
XbaI T'CTAG_A
Cuts at: 3730 3730
Size: 6394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2446 2533 3560 2200
Size: 246 87 1027 5034
Fragments arranged by size:
5034 1027 246 87
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 3719 1870
Size: 1849 4545
XmnI GAAnn'nnTTC
Cuts at: 4523 4523
Size: 6394
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2229 3469 3630 4202
Size: 53 83 186 936 695 1240 161 572
Cuts at: 4202 276
Size: 2468
Fragments arranged by size:
2468 1240 936 695 572 186 161 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI