Restriction map for
pFN21AB3027
(Circular) (Six-base) MAPSORT of: pFN21AB3027.seq Check: 9341 from: 1 to: 8347
pFN21AB3027 8347 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3167 3702 4350 3167
Size: 535 648 7164
Fragments arranged by size:
7164 648 535
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6157 278
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2097 4965 5690 1937
Size: 160 2868 725 4594
Fragments arranged by size:
4594 2868 725 160
Acc65I G'GTAC_C
Cuts at: 3988 5668 3988
Size: 1680 6667
AclI AA'CG_TT
Cuts at: 1563 6474 6847 1563
Size: 4911 373 3063
Fragments arranged by size:
4911 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3662 3946 4311 4909 5025 6410 7794 8062
Size: 2030 284 365 598 116 1385 1384 268
Cuts at: 8062 1632
Size: 1917
Fragments arranged by size:
2030 1917 1385 1384 598 365 284 268
116
AfeI AGC'GCT
Cuts at: 5035 5035
Size: 8347
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8150
AflIII A'CryG_T
Cuts at: 1203 2886 4635 7246 8223 1203
Size: 1683 1749 2611 977 1327
Fragments arranged by size:
2611 1749 1683 1327 977
AgeI A'CCGG_T
Cuts at: 2601 7178 2601
Size: 4577 3770
AhdI GACnn_n'nnGTC
Cuts at: 5008 7076 5008
Size: 2068 6279
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4197 5624 1184
Size: 3013 1427 3907
Fragments arranged by size:
3907 3013 1427
AlwNI CAG_nnn'CTG
Cuts at: 2950 3530 3622 3633 4113 5006 5141 5473 6090
Size: 580 92 11 480 893 135 332 617
Cuts at: 6090 7662 2950
Size: 1572 3635
Fragments arranged by size:
3635 1572 893 617 580 480 332 135
92 11
ApaI G_GGCC'C
Cuts at: 3052 3754 4794 3052
Size: 702 1040 6605
Fragments arranged by size:
6605 1040 702
ApaLI G'TGCA_C
Cuts at: 2903 6404 7560 2903
Size: 3501 1156 3690
Fragments arranged by size:
3690 3501 1156
ApoI r'AATT_y
Cuts at: 1493 4069 5656 5882 6034 8143 1493
Size: 2576 1587 226 152 2109 1697
Fragments arranged by size:
2576 2109 1697 1587 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6901 160
Size: 872 5869 1606
Fragments arranged by size:
5869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
AvaI C'yCGr_G
Cuts at: 1870 1943 2381 4522 5097 5331 5418 5672 1870
Size: 73 438 2141 575 234 87 254 4545
Fragments arranged by size:
4545 2141 575 438 254 234 87 73
BamHI G'GATC_C
Cuts at: 3072 5677 6044 3072
Size: 2605 367 5375
Fragments arranged by size:
5375 2605 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2199 3017 3293 3302 3988
Size: 325 217 627 412 818 276 9 686
Cuts at: 3988 4203 5668 7123 618
Size: 215 1465 1455 1842
Fragments arranged by size:
1842 1465 1455 818 686 627 412 325
276 217 215 9
BanII G_rGCy'C
Cuts at: 1391 3052 3185 3443 3754 3808 4794 4847 4865
Size: 1661 133 258 311 54 986 53 18
Cuts at: 4865 5590 5666 1391
Size: 725 76 4072
Fragments arranged by size:
4072 1661 986 725 311 258 133 76
54 53 18
BbeI G_GCGC'C
Cuts at: 2203 3297 4207 2203
Size: 1094 910 6343
Fragments arranged by size:
6343 1094 910
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2033 2708 3094 4709 4899 928
Size: 971 134 675 386 1615 190 4376
Fragments arranged by size:
4376 1615 971 675 386 190 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2600 2634 2786 2820 4376 4410 6538
Size: 34 663 34 152 34 1556 34 2128
Cuts at: 6538 6572 1903
Size: 34 3678
Fragments arranged by size:
3678 2128 1556 663 152 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5122 6239 7449 5122
Size: 1117 1210 6020
Fragments arranged by size:
6020 1210 1117
BclI T'GATC_A
Cuts at: 1548 4437 5310 1548
Size: 2889 873 4585
Fragments arranged by size:
4585 2889 873
BfrBI ATG'CAT
Cuts at: 4246 4246
Size: 8347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3251 4723 4732
Size: 107 122 71 631 605 1579 1472 9
Cuts at: 4732 4776 6958 136
Size: 44 2182 1525
Fragments arranged by size:
2182 1579 1525 1472 631 605 122 107
71 44 9
BglII A'GATC_T
Cuts at: 2733 8342 2733
Size: 5609 2738
BlpI GC'TnA_GC
Cuts at: 5764 5764
Size: 8347
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2907 2998 3005 3052 3546 3754 4794
Size: 285 832 91 7 47 494 208 1040
Cuts at: 4794 5105 5168 5373 6408 7564 1790
Size: 311 63 205 1035 1156 2573
Fragments arranged by size:
2573 1156 1040 1035 832 494 311 285
208 205 91 63 47 7
BmgBI CAC'GTC
Cuts at: 1318 4496 1318
Size: 3178 5169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3757 4848 6078
Size: 416 53 83 399 24 2340 1091 1230
Cuts at: 6078 7036 442
Size: 958 1753
Fragments arranged by size:
2340 1753 1230 1091 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2538 5149 5173 5444 2538
Size: 2611 24 271 5441
Fragments arranged by size:
5441 2611 271 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2780 3336 3457 4287 4346 4394 5399 5555
Size: 1541 556 121 830 59 48 1005 156
Cuts at: 5555 7007 1239
Size: 1452 2579
Fragments arranged by size:
2579 1541 1452 1005 830 556 156 121
59 48
Bpu10I CC'TnA_GC
Cuts at: 3184 4506 3184
Size: 1322 7025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3752 4103 6472 7337 7635 3752
Size: 351 2369 865 298 4464
Fragments arranged by size:
4464 2369 865 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 2251 5624 7010 882
Size: 617 576 176 3373 1386 2219
Fragments arranged by size:
3373 2219 1386 617 576 176
BsaAI yAC'GTr
Cuts at: 493 1204 6138 493
Size: 711 4934 2702
Fragments arranged by size:
4934 2702 711
BsaBI GATnn'nnATC
Cuts at: 8341 8341
Size: 8347
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2200 3294 4204 6154
Size: 53 83 186 936 667 1094 910 1950
Cuts at: 6154 6536 7171 275
Size: 382 635 1451
Fragments arranged by size:
1950 1451 1094 936 910 667 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2601 5046 6780 7178 7452 7599 8010 2601
Size: 2445 1734 398 274 147 411 2938
Fragments arranged by size:
2938 2445 1734 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 1178
Size: 30 165 30 1350 30 6742
Fragments arranged by size:
6742 1350 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2639 2675 3413 3428 3431 4100 4607
Size: 1213 240 36 738 15 3 669 507
Cuts at: 4607 1186
Size: 4926
Fragments arranged by size:
4926 1213 738 669 507 240 36 15
3
BseYI C'CCAG_C
Cuts at: 2212 2231 2289 3045 3246 4766 4797 5302 7550
Size: 19 58 756 201 1520 31 505 2248
Cuts at: 7550 8046 2212
Size: 496 2513
Fragments arranged by size:
2513 2248 1520 756 505 496 201 58
31 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3608 1163
Size: 573 1872 5902
Fragments arranged by size:
5902 1872 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5797 6558 7586 1540
Size: 275 186 3796 761 1028 2301
Fragments arranged by size:
3796 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2519 2907 3132 3443 3808 4191 4865 6408 6493
Size: 388 225 311 365 383 674 1543 85
Cuts at: 6493 7564 2519
Size: 1071 3302
Fragments arranged by size:
3302 1543 1071 674 388 383 365 311
225 85
BsmI GAATG_Cn'
Cuts at: 4399 5858 5951 4399
Size: 1459 93 6795
Fragments arranged by size:
6795 1459 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3848 4229 4481 3848
Size: 381 252 7714
Fragments arranged by size:
7714 381 252
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2519 2907 2998 3005 3052 3132
Size: 399 285 444 388 91 7 47 80
Cuts at: 3132 3185 3443 3546 3754 3808 4191 4794 4847
Size: 53 258 103 208 54 383 603 53
Cuts at: 4847 4865 5105 5168 5373 5590 5666 6408 6493
Size: 18 240 63 205 217 76 742 85
Cuts at: 6493 7564 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 603 444 399 388 383
285 258 240 217 208 205 103 91
85 80 76 63 54 53 53 47
18 7
BspEI T'CCGG_A
Cuts at: 8010 8010
Size: 8347
BspHI T'CATG_A
Cuts at: 2685 4809 6236 7966 2685
Size: 2124 1427 1730 3066
Fragments arranged by size:
3066 2124 1730 1427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2093 3167 3702 4350 5702 844
Size: 1249 1074 535 648 1352 3489
Fragments arranged by size:
3489 1352 1249 1074 648 535
BsrBI CCG'CTC
Cuts at: 1394 1823 4312 4378 4419 4510 6234 1394
Size: 429 2489 66 41 91 1724 3507
Fragments arranged by size:
3507 2489 1724 429 91 66 41
BsrDI GCAATG_nn'
Cuts at: 65 1248 2848 2872 3668 6842 7016 65
Size: 1183 1600 24 796 3174 174 1396
Fragments arranged by size:
3174 1600 1396 1183 796 174 24
BsrFI r'CCGG_y
Cuts at: 1700 1810 2601 3970 4731 6991 7178 1700
Size: 110 791 1369 761 2260 187 2869
Fragments arranged by size:
2869 2260 1369 791 761 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8347
BssSI C'ACGA_G
Cuts at: 2979 3815 5267 6407 7419 2979
Size: 836 1452 1140 1012 3907
Fragments arranged by size:
3907 1452 1140 1012 836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3201 3622 5473 794
Size: 2407 421 1851 3668
Fragments arranged by size:
3668 2407 1851 421
BstBI TT'CG_AA
Cuts at: 7151 7151
Size: 8347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8347
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3147 3254 2011
Size: 1136 107 7104
Fragments arranged by size:
7104 1136 107
BstYI r'GATC_y
Cuts at: 1972 2733 3072 3501 5677 6044 6429 6446 7898
Size: 761 339 429 2176 367 385 17 1452
Cuts at: 7898 7984 7996 8342 1972
Size: 86 12 346 1977
Fragments arranged by size:
2176 1977 1452 761 429 385 367 346
339 86 17 12
BstZ17I GTA'TAC
Cuts at: 4966 4966
Size: 8347
Bsu36I CC'TnA_GG
Cuts at: 2937 3078 5134 2937
Size: 141 2056 6150
Fragments arranged by size:
6150 2056 141
BtgI C'CryG_G
Cuts at: 513 1065 2004 3879 4020 4771 5160 5173 5350
Size: 552 939 1875 141 751 389 13 177
Cuts at: 5350 513
Size: 3510
Fragments arranged by size:
3510 1875 939 751 552 389 177 141
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7608
Fragments arranged by size:
7608 683 56
BtsI GCAGTG_nn'
Cuts at: 4164 4457 5128 5474 5632 5863 6657 6677 4164
Size: 293 671 346 158 231 794 20 5834
Fragments arranged by size:
5834 794 671 346 293 231 158 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8312
DraI TTT'AAA
Cuts at: 5651 5651
Size: 8347
DraIII CAC_nnn'GTG
Cuts at: 4427 4427
Size: 8347
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7354 809
Size: 507 6038 1802
Fragments arranged by size:
6038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 2585 2898
Size: 54 1425 50 275 18 487 268 313
Cuts at: 2898 5170 5794 6683 8312 8
Size: 2272 624 889 1629 43
Fragments arranged by size:
2272 1629 1425 889 624 487 313 275
268 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5794 1537
Size: 275 3982 4090
Fragments arranged by size:
4090 3982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2882 3596 4302 4946 5195 5447 6277 1353
Size: 1529 714 706 644 249 252 830 3423
Fragments arranged by size:
3423 1529 830 714 706 644 252 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4181 4480 6934 7308 7454 387
Size: 1064 138 2592 299 2454 374 146 1280
Fragments arranged by size:
2592 2454 1280 1064 374 299 146 138
EcoICRI GAG'CTC
Cuts at: 3441 3806 4863 3441
Size: 365 1057 6925
Fragments arranged by size:
6925 1057 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2780 3336 3457 3662 3946 4287 4311
Size: 393 1148 556 121 205 284 341 24
Cuts at: 4311 4346 4394 4909 5025 5399 5555 6410 7007
Size: 35 48 515 116 374 156 855 597
Cuts at: 7007 7794 8062 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1148 855 787 597 556 515 393
374 341 284 268 205 156 121 116
48 35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 5519 5581 5519
Size: 62 8285
EcoO109I rG'GnC_Cy
Cuts at: 2389 3189 3664 3751 4628 4790 4791 5512 2389
Size: 800 475 87 877 162 1 721 5224
Fragments arranged by size:
5224 877 800 721 475 162 87 1
EcoRI G'AATT_C
Cuts at: 5656 6034 5656
Size: 378 7969
EcoRV GAT'ATC
Cuts at: 2683 2683
Size: 8347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4063 4975 4063
Size: 912 7435
FseI GG_CCGG'CC
Cuts at: 4735 4735
Size: 8347
FspI TGC'GCA
Cuts at: 6853 6853
Size: 8347
HaeII r_GCGC'y
Cuts at: 1678 2203 3046 3297 4207 5037 7494 1678
Size: 525 843 251 910 830 2457 2531
Fragments arranged by size:
2531 2457 910 843 830 525 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2753 2785 4994
Size: 32 135 32 773 32 376 32 2209
Cuts at: 4994 5026 5228 5260 5302 5334 6988 7020 7062
Size: 32 202 32 42 32 1654 32 42
Cuts at: 7062 7094 1373
Size: 32 2626
Fragments arranged by size:
2626 2209 1654 773 376 202 135 42
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5691 5937 669
Size: 988 281 3753 246 3079
Fragments arranged by size:
3753 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4469 748
Size: 3721 4626
HpaI GTT'AAC
Cuts at: 5937 5937
Size: 8347
KasI G'GCGC_C
Cuts at: 2199 3293 4203 2199
Size: 1094 910 6343
Fragments arranged by size:
6343 1094 910
KpnI G_GTAC'C
Cuts at: 3992 5672 3992
Size: 1680 6667
MfeI C'AATT_G
Cuts at: 5946 5946
Size: 8347
MluI A'CGCG_T
Cuts at: 8223 8223
Size: 8347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 2678 5574 7461 7645 1305
Size: 1203 170 2896 1887 184 2007
Fragments arranged by size:
2896 2007 1887 1203 184 170
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6525
Fragments arranged by size:
6525 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2068 2502 3269 3884 5366 6305
Size: 648 843 59 434 767 615 1482 939
Cuts at: 6305 6664 6823 518
Size: 359 159 2042
Fragments arranged by size:
2042 1482 939 843 767 648 615 434
359 159 59
MspA1I CmG'CkG
Cuts at: 1606 2432 3781 3892 4709 4929 5175 5251 5763
Size: 826 1349 111 817 220 246 76 512
Cuts at: 5763 6440 7588 7833 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1349 1148 826 817 677 512 246
245 220 111 76
NaeI GCC'GGC
Cuts at: 4733 4733
Size: 8347
NarI GG'CG_CC
Cuts at: 2200 3294 4204 2200
Size: 1094 910 6343
Fragments arranged by size:
6343 1094 910
NcoI C'CATG_G
Cuts at: 513 1065 2004 3879 4020 5350 513
Size: 552 939 1875 141 1330 3510
Fragments arranged by size:
3510 1875 1330 939 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8347
NgoMIV G'CCGG_C
Cuts at: 4731 4731
Size: 8347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8347
NotI GC'GGCC_GC
Cuts at: 5794 5794
Size: 8347
NsiI A_TGCA'T
Cuts at: 4248 4248
Size: 8347
NspI r_CATG'y
Cuts at: 1132 1229 2890 3243 4246 4639 5705 7250 1132
Size: 97 1661 353 1003 393 1066 1545 2229
Fragments arranged by size:
2229 1661 1545 1066 1003 393 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8347
PciI A'CATG_T
Cuts at: 2886 4635 7246 2886
Size: 1749 2611 3987
Fragments arranged by size:
3987 2611 1749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2296 4261 4919 5191 1108
Size: 1188 1965 658 272 4264
Fragments arranged by size:
4264 1965 1188 658 272
PfoI T'CCnGG_A
Cuts at: 3473 3473
Size: 8347
PmeI GTTT'AAAC
Cuts at: 5651 5651
Size: 8347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6416 6448 7929 7961 6416
Size: 32 1481 32 6802
Fragments arranged by size:
6802 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3664 4628 5512 3664
Size: 964 884 6499
Fragments arranged by size:
6499 964 884
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8347
PsiI TTA'TAA
Cuts at: 5917 5917
Size: 8347
PspOMI G'GGCC_C
Cuts at: 3048 3750 4790 3048
Size: 702 1040 6605
Fragments arranged by size:
6605 1040 702
PspXI vC'TCGA_Gb
Cuts at: 4522 4522
Size: 8347
PstI C_TGCA'G
Cuts at: 830 2623 2970 3687 4982 5595 5699 830
Size: 1793 347 717 1295 613 104 3478
Fragments arranged by size:
3478 1793 1295 717 613 347 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8347
PvuII CAG'CTG
Cuts at: 2432 3892 4709 2432
Size: 1460 817 6070
Fragments arranged by size:
6070 1460 817
SacI G_AGCT'C
Cuts at: 3443 3808 4865 3443
Size: 365 1057 6925
Fragments arranged by size:
6925 1057 365
SacII CC_GC'GG
Cuts at: 5176 5176
Size: 8347
SalI G'TCGA_C
Cuts at: 1936 5689 1936
Size: 3753 4594
SanDI GG'GwC_CC
Cuts at: 3664 4628 3664
Size: 964 7383
SapI GCTCTTCn'nnn_
Cuts at: 3596 4302 3596
Size: 706 7641
SbfI CC_TGCA'GG
Cuts at: 5699 5699
Size: 8347
ScaI AGT'ACT
Cuts at: 6595 8307 6595
Size: 1712 6635
SfcI C'TryA_G
Cuts at: 826 1045 2619 2966 3683 4978 5591 5695 6830
Size: 219 1574 347 717 1295 613 104 1135
Cuts at: 6830 7511 7702 826
Size: 681 191 1471
Fragments arranged by size:
1574 1471 1295 1135 717 681 613 347
219 191 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 4732 4732
Size: 8347
SfoI GGC'GCC
Cuts at: 2201 3295 4205 2201
Size: 1094 910 6343
Fragments arranged by size:
6343 1094 910
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8347
SmaI CCC'GGG
Cuts at: 1872 5674 1872
Size: 3802 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3767 4118 4522 6451 7352 7614
Size: 197 926 1824 351 404 1929 901 262
Cuts at: 7614 820
Size: 1553
Fragments arranged by size:
1929 1824 1553 926 901 404 351 262
197
SnaBI TAC'GTA
Cuts at: 493 6138 493
Size: 5645 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8347
SphI G_CATG'C
Cuts at: 1132 4246 5705 1132
Size: 3114 1459 3774
Fragments arranged by size:
3774 3114 1459
SspI AAT'ATT
Cuts at: 5 52 6271 5
Size: 47 6219 2081
Fragments arranged by size:
6219 2081 47
StuI AGG'CCT
Cuts at: 1517 4623 5153 1517
Size: 3106 530 4711
Fragments arranged by size:
4711 3106 530
StyI C'CwwG_G
Cuts at: 513 1065 2004 3462 3879 3924 4020 4131 5350
Size: 552 939 1458 417 45 96 111 1219
Cuts at: 5350 5786 513
Size: 436 3074
Fragments arranged by size:
3074 1458 1219 939 552 436 417 111
96 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2769 5489 6374 6391 6544 6729
Size: 1255 665 524 2720 885 17 153 185
Cuts at: 6729 7174 325
Size: 445 1498
Fragments arranged by size:
2720 1498 1255 885 665 524 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5566 6593 8305
Size: 274 80 33 51 1443 3589 1027 1712
Cuts at: 8305 96
Size: 138
Fragments arranged by size:
3589 1712 1443 1027 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3486 3518 5255 5287 8307 8339 3486
Size: 32 1737 32 3020 32 3494
Fragments arranged by size:
3494 3020 1737 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8347
XbaI T'CTAG_A
Cuts at: 5683 5683
Size: 8347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3144 4262 5485 3144
Size: 1118 1223 6006
Fragments arranged by size:
6006 1223 1118
XhoI C'TCGA_G
Cuts at: 1943 4522 1943
Size: 2579 5768
XmaI C'CCGG_G
Cuts at: 1870 5672 1870
Size: 3802 4545
XmnI GAAnn'nnTTC
Cuts at: 2030 4955 6476 2030
Size: 2925 1521 3901
Fragments arranged by size:
3901 2925 1521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6155 276
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI FspAI NruI
PacI PmlI PsrI RsrII SexAI SrfI SwaI