Restriction map for
pFN21AB3021
(Circular) (Six-base) MAPSORT of: pFN21AB3021.seq Check: 4631 from: 1 to: 7489
pFN21AB3021 7489 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5299 278
Size: 53 83 186 936 3763 2468
Fragments arranged by size:
3763 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3247 4832 1937
Size: 1310 1585 4594
Fragments arranged by size:
4594 1585 1310
Acc65I G'GTAC_C
Cuts at: 4810 4810
Size: 7489
AclI AA'CG_TT
Cuts at: 1563 5616 5989 1563
Size: 4053 373 3063
Fragments arranged by size:
4053 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2473 3017 3177 4451 4614 5552 6936 7204
Size: 841 544 160 1274 163 938 1384 268
Cuts at: 7204 1632
Size: 1917
Fragments arranged by size:
1917 1384 1274 938 841 544 268 163
160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7292
AflIII A'CryG_T
Cuts at: 1203 2507 3863 4225 6388 7365 1203
Size: 1304 1356 362 2163 977 1327
Fragments arranged by size:
2163 1356 1327 1304 977 362
AgeI A'CCGG_T
Cuts at: 6320 6320
Size: 7489
AhdI GACnn_n'nnGTC
Cuts at: 6218 6218
Size: 7489
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7489
AlwNI CAG_nnn'CTG
Cuts at: 4294 4705 5232 6804 4294
Size: 411 527 1572 4979
Fragments arranged by size:
4979 1572 527 411
ApaLI G'TGCA_C
Cuts at: 2784 5546 6702 2784
Size: 2762 1156 3571
Fragments arranged by size:
3571 2762 1156
ApoI r'AATT_y
Cuts at: 1493 2393 2560 2887 2905 2973 3172 3290 3418
Size: 900 167 327 18 68 199 118 128
Cuts at: 3418 3871 4163 4446 4691 4798 5024 5176 7285
Size: 453 292 283 245 107 226 152 2109
Cuts at: 7285 1493
Size: 1697
Fragments arranged by size:
2109 1697 900 453 327 292 283 245
226 199 167 152 128 118 107 68
18
AseI AT'TA_AT
Cuts at: 160 1032 2742 3158 6043 160
Size: 872 1710 416 2885 1606
Fragments arranged by size:
2885 1710 1606 872 416
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7489
AvaI C'yCGr_G
Cuts at: 1870 1943 4814 1870
Size: 73 2871 4545
Fragments arranged by size:
4545 2871 73
BamHI G'GATC_C
Cuts at: 4819 5186 4819
Size: 367 7122
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 4810 6265 618
Size: 325 217 627 281 2742 1455 1842
Fragments arranged by size:
2742 1842 1455 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 4808 1391
Size: 3417 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4273 928
Size: 971 2374 4144
Fragments arranged by size:
4144 2374 971
BbvCI CC'TCA_GC
Cuts at: 3904 3904
Size: 7489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5680 5714 1903
Size: 34 50 34 3659 34 3678
Fragments arranged by size:
3678 3659 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4759 5381 6591 3266
Size: 1493 622 1210 4164
Fragments arranged by size:
4164 1493 1210 622
BclI T'GATC_A
Cuts at: 1548 3184 3754 4233 1548
Size: 1636 570 479 4804
Fragments arranged by size:
4804 1636 570 479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6100 136
Size: 107 122 71 631 605 4428 1525
Fragments arranged by size:
4428 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2992 3836 7484 2992
Size: 844 3648 2997
Fragments arranged by size:
3648 2997 844
BlpI GC'TnA_GC
Cuts at: 4906 4906
Size: 7489
Bme1580I G_kGCm'C
Cuts at: 1790 2788 5550 6706 1790
Size: 998 2762 1156 2573
Fragments arranged by size:
2762 2573 1156 998
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7489
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5220 6178 442
Size: 416 53 83 399 24 3803 958 1753
Fragments arranged by size:
3803 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3977 1056
Size: 2921 4568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3108 3776 4425 6149 1239
Size: 1869 668 649 1724 2579
Fragments arranged by size:
2579 1869 1724 668 649
Bpu10I CC'TnA_GC
Cuts at: 3904 3904
Size: 7489
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 3497 5614 6479 6777 2165
Size: 1332 2117 865 298 2877
Fragments arranged by size:
2877 2117 1332 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6152 882
Size: 617 4653 2219
Fragments arranged by size:
4653 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5280 493
Size: 711 4076 2702
Fragments arranged by size:
4076 2702 711
BsaBI GATnn'nnATC
Cuts at: 3819 7483 3819
Size: 3664 3825
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5296 5678 6313 275
Size: 53 83 186 936 3763 382 635 1451
Fragments arranged by size:
3763 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5922 6320 6594 6741 7152 5922
Size: 398 274 147 411 6259
Fragments arranged by size:
6259 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7264
Fragments arranged by size:
7264 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 4259 1186
Size: 922 2151 4416
Fragments arranged by size:
4416 2151 922
BseYI C'CCAG_C
Cuts at: 2534 6692 7188 2534
Size: 4158 496 2835
Fragments arranged by size:
4158 2835 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3208 1163
Size: 573 1472 5444
Fragments arranged by size:
5444 1472 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 4573 4939 5700 6728 1540
Size: 275 186 33 2539 366 761 1028 2301
Fragments arranged by size:
2539 2301 1028 761 366 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2788 4023 4581 5550 5635 6706 2788
Size: 1235 558 969 85 1071 3571
Fragments arranged by size:
3571 1235 1071 969 558 85
BsmI GAATG_Cn'
Cuts at: 4740 5000 5093 4740
Size: 260 93 7136
Fragments arranged by size:
7136 260 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2788 4023 4581 4808 5550 5635 6706
Size: 399 998 1235 558 227 742 85 1071
Cuts at: 6706 1391
Size: 2174
Fragments arranged by size:
2174 1235 1071 998 742 558 399 227
85
BspEI T'CCGG_A
Cuts at: 7152 7152
Size: 7489
BspHI T'CATG_A
Cuts at: 3019 4086 5378 7108 3019
Size: 1067 1292 1730 3400
Fragments arranged by size:
3400 1730 1292 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 4844 844
Size: 1292 2708 3489
Fragments arranged by size:
3489 2708 1292
BsrBI CCG'CTC
Cuts at: 1394 1823 5376 1394
Size: 429 3553 3507
Fragments arranged by size:
3553 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5984 6158 65
Size: 1183 4736 174 1396
Fragments arranged by size:
4736 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6133 6320 1700
Size: 110 4323 187 2869
Fragments arranged by size:
4323 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7489
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7489
BssSI C'ACGA_G
Cuts at: 5549 6561 5549
Size: 1012 6477
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4066 794
Size: 3272 4217
BstBI TT'CG_AA
Cuts at: 6293 6293
Size: 7489
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7489
BstYI r'GATC_y
Cuts at: 1972 2992 3836 4192 4819 5186 5571 5588 7040
Size: 1020 844 356 627 367 385 17 1452
Cuts at: 7040 7126 7138 7484 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1020 844 627 385 367 356
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3248 3248
Size: 7489
Bsu36I CC'TnA_GG
Cuts at: 2091 2091
Size: 7489
BtgI C'CryG_G
Cuts at: 513 1065 2055 3548 513
Size: 552 990 1493 4454
Fragments arranged by size:
4454 1493 990 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6750
Fragments arranged by size:
6750 683 56
BtsI GCAGTG_nn'
Cuts at: 3804 5005 5799 5819 3804
Size: 1201 794 20 5474
Fragments arranged by size:
5474 1201 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7454
DraI TTT'AAA
Cuts at: 3171 3283 3573 4793 3171
Size: 112 290 1220 5867
Fragments arranged by size:
5867 1220 290 112
DraIII CAC_nnn'GTG
Cuts at: 4001 4001
Size: 7489
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6496 809
Size: 507 5180 1802
Fragments arranged by size:
5180 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 3489 4936
Size: 54 1425 50 275 18 201 1458 1447
Cuts at: 4936 5825 7454 8
Size: 889 1629 43
Fragments arranged by size:
1629 1458 1447 1425 889 275 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 4936 1537
Size: 275 219 2905 4090
Fragments arranged by size:
4090 2905 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 3036 3090 4236 4427 4776 5419 1353
Size: 1139 544 54 1146 191 349 643 3423
Fragments arranged by size:
3423 1146 1139 643 544 349 191 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6076 6450 6596 387
Size: 1064 138 4487 374 146 1280
Fragments arranged by size:
4487 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2473 3017 3108 3177 3776 4425 4451
Size: 393 841 544 91 69 599 649 26
Cuts at: 4451 4614 5552 6149 6936 7204 1239
Size: 163 938 597 787 268 1524
Fragments arranged by size:
1524 938 841 787 649 599 597 544
393 268 163 91 69 26
EcoO109I rG'GnC_Cy
Cuts at: 2098 2125 4329 2098
Size: 27 2204 5258
Fragments arranged by size:
5258 2204 27
EcoRI G'AATT_C
Cuts at: 2905 4798 5176 2905
Size: 1893 378 5218
Fragments arranged by size:
5218 1893 378
EcoRV GAT'ATC
Cuts at: 2547 2547
Size: 7489
FspI TGC'GCA
Cuts at: 5995 5995
Size: 7489
HaeII r_GCGC'y
Cuts at: 1678 6636 1678
Size: 4958 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2191 2223 2327 2359 4398
Size: 32 135 32 619 32 104 32 2039
Cuts at: 4398 4430 4479 4511 4708 4740 6130 6162 6204
Size: 32 49 32 197 32 1390 32 42
Cuts at: 6204 6236 1373
Size: 32 2626
Fragments arranged by size:
2626 2039 1390 619 197 135 104 49
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2232 2329 4833 5079 669
Size: 988 281 294 97 2504 246 3079
Fragments arranged by size:
3079 2504 988 294 281 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7489
HpaI GTT'AAC
Cuts at: 2232 5079 2232
Size: 2847 4642
KpnI G_GTAC'C
Cuts at: 4814 4814
Size: 7489
MfeI C'AATT_G
Cuts at: 4506 5088 4506
Size: 582 6907
MluI A'CGCG_T
Cuts at: 3863 7365 3863
Size: 3502 3987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3175 6603 6787 1305
Size: 1870 3428 184 2007
Fragments arranged by size:
3428 2007 1870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3491 10
Size: 54 1425 343 1659 4008
Fragments arranged by size:
4008 1659 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5447 5806 5965 518
Size: 648 4281 359 159 2042
Fragments arranged by size:
4281 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2057 2115 2729 4905 5582 6730 6975
Size: 442 9 58 614 2176 677 1148 245
Cuts at: 6975 1606
Size: 2120
Fragments arranged by size:
2176 2120 1148 677 614 442 245 58
9
NcoI C'CATG_G
Cuts at: 513 1065 3548 513
Size: 552 2483 4454
Fragments arranged by size:
4454 2483 552
NdeI CA'TA_TG
Cuts at: 387 3453 4136 387
Size: 3066 683 3740
Fragments arranged by size:
3740 3066 683
NheI G'CTAG_C
Cuts at: 1052 3973 1052
Size: 2921 4568
NotI GC'GGCC_GC
Cuts at: 4936 4936
Size: 7489
NspI r_CATG'y
Cuts at: 1132 1229 2511 4847 6392 1132
Size: 97 1282 2336 1545 2229
Fragments arranged by size:
2336 2229 1545 1282 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7489
PciI A'CATG_T
Cuts at: 2507 6388 2507
Size: 3881 3608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7489
PfoI T'CCnGG_A
Cuts at: 4402 4402
Size: 7489
PmeI GTTT'AAAC
Cuts at: 4793 4793
Size: 7489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2330 2362 5558 5590 7071 7103 2330
Size: 32 3196 32 1481 32 2716
Fragments arranged by size:
3196 2716 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2125 2098
Size: 27 7462
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7489
PsiI TTA'TAA
Cuts at: 3957 5059 3957
Size: 1102 6387
PstI C_TGCA'G
Cuts at: 830 3811 4071 4841 830
Size: 2981 260 770 3478
Fragments arranged by size:
3478 2981 770 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7489
PvuII CAG'CTG
Cuts at: 2729 2729
Size: 7489
SacII CC_GC'GG
Cuts at: 2058 2058
Size: 7489
SalI G'TCGA_C
Cuts at: 1936 4831 1936
Size: 2895 4594
SbfI CC_TGCA'GG
Cuts at: 4841 4841
Size: 7489
ScaI AGT'ACT
Cuts at: 5737 7449 5737
Size: 1712 5777
SfcI C'TryA_G
Cuts at: 826 1045 2363 3045 3807 4067 4837 5972 6653
Size: 219 1318 682 762 260 770 1135 681
Cuts at: 6653 6844 826
Size: 191 1471
Fragments arranged by size:
1471 1318 1135 770 762 682 681 260
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7489
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7489
SmaI CCC'GGG
Cuts at: 1872 4816 1872
Size: 2944 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 3476 5593 6494 6756 820
Size: 197 926 201 1332 2117 901 262 1553
Fragments arranged by size:
2117 1553 1332 926 901 262 201 197
SnaBI TAC'GTA
Cuts at: 493 5280 493
Size: 4787 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7489
SphI G_CATG'C
Cuts at: 1132 4847 1132
Size: 3715 3774
SspI AAT'ATT
Cuts at: 5 52 4306 5413 5
Size: 47 4254 1107 2081
Fragments arranged by size:
4254 2081 1107 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7489
StyI C'CwwG_G
Cuts at: 513 1065 3548 4928 513
Size: 552 2483 1380 3074
Fragments arranged by size:
3074 2483 1380 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 3753 4038 4267 5516 5533 5686
Size: 1255 1196 977 285 229 1249 17 153
Cuts at: 5686 5871 6316 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1249 1196 977 445 285 229
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3506 5735 7447
Size: 274 80 33 51 1443 1529 2229 1712
Cuts at: 7447 96
Size: 138
Fragments arranged by size:
2229 1712 1529 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3217 3249 7449 7481 3217
Size: 32 4200 32 3225
Fragments arranged by size:
4200 3225 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7489
XbaI T'CTAG_A
Cuts at: 2995 4825 2995
Size: 1830 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7489
XmaI C'CCGG_G
Cuts at: 1870 4814 1870
Size: 2944 4545
XmnI GAAnn'nnTTC
Cuts at: 4008 5618 4008
Size: 1610 5879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5297 276
Size: 53 83 186 936 3763 2468
Fragments arranged by size:
3763 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI