Restriction map for
pFN21AB3016
(Circular) (Six-base) MAPSORT of: pFN21AB3016.seq Check: 9542 from: 1 to: 7042
pFN21AB3016 7042 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4224 4852 278
Size: 53 83 186 936 2688 628 2468
Fragments arranged by size:
2688 2468 936 628 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4041 4385 1937
Size: 2104 344 4594
Fragments arranged by size:
4594 2104 344
Acc65I G'GTAC_C
Cuts at: 4363 4363
Size: 7042
AclI AA'CG_TT
Cuts at: 1563 3920 5169 5542 1563
Size: 2357 1249 373 3063
Fragments arranged by size:
3063 2357 1249 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2382 3192 5105 6489 6757 1632
Size: 750 810 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 810 750 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6845
AflIII A'CryG_T
Cuts at: 1203 3491 5941 6918 1203
Size: 2288 2450 977 1327
Fragments arranged by size:
2450 2288 1327 977
AgeI A'CCGG_T
Cuts at: 5873 5873
Size: 7042
AhdI GACnn_n'nnGTC
Cuts at: 5771 5771
Size: 7042
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7042
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 3936 3968 2388
Size: 32 1516 32 5462
Fragments arranged by size:
5462 1516 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2897 4785 6357 2897
Size: 1888 1572 3582
Fragments arranged by size:
3582 1888 1572
ApaLI G'TGCA_C
Cuts at: 5099 6255 5099
Size: 1156 5886
ApoI r'AATT_y
Cuts at: 1493 2501 2723 4106 4351 4577 4729 6838 1493
Size: 1008 222 1383 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 1383 1008 245 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 5596 160
Size: 872 4564 1606
Fragments arranged by size:
4564 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
AvaI C'yCGr_G
Cuts at: 1870 1943 4367 1870
Size: 73 2424 4545
Fragments arranged by size:
4545 2424 73
BamHI G'GATC_C
Cuts at: 2384 3361 4372 4739 2384
Size: 977 1011 367 4687
Fragments arranged by size:
4687 1011 977 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3720 4363 5818 618
Size: 325 217 627 248 1685 643 1455 1842
Fragments arranged by size:
1842 1685 1455 643 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 3709 4279 4361 1391
Size: 2318 570 82 4072
Fragments arranged by size:
4072 2318 570 82
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 7042
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3565 928
Size: 971 1666 4405
Fragments arranged by size:
4405 1666 971
BbvCI CC'TCA_GC
Cuts at: 3970 3970
Size: 7042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5233 5267 1903
Size: 34 3296 34 3678
Fragments arranged by size:
3678 3296 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2390 4934 6144 2390
Size: 2544 1210 3288
Fragments arranged by size:
3288 2544 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7042
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2044 3195 5653
Size: 107 122 71 631 605 372 1151 2458
Cuts at: 5653 136
Size: 1525
Fragments arranged by size:
2458 1525 1151 631 605 372 122 107
71
BglII A'GATC_T
Cuts at: 3529 3905 7037 3529
Size: 376 3132 3534
Fragments arranged by size:
3534 3132 376
BlpI GC'TnA_GC
Cuts at: 4459 4459
Size: 7042
Bme1580I G_kGCm'C
Cuts at: 1790 5103 6259 1790
Size: 3313 1156 2573
Fragments arranged by size:
3313 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2133 4142 1318
Size: 815 2009 4218
Fragments arranged by size:
4218 2009 815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3944 4773 5731
Size: 416 53 83 399 24 2527 829 958
Cuts at: 5731 442
Size: 1753
Fragments arranged by size:
2527 1753 958 829 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7042
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 7042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2841 3164 3666 3693 5702 1239
Size: 821 781 323 502 27 2009 2579
Fragments arranged by size:
2579 2009 821 781 502 323 27
Bpu10I CC'TnA_GC
Cuts at: 3970 3970
Size: 7042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4307 5167 6032 6330 4307
Size: 860 865 298 5019
Fragments arranged by size:
5019 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2064 2478 3824 5705 882
Size: 617 565 414 1346 1881 2219
Fragments arranged by size:
2219 1881 1346 617 565 414
BsaAI yAC'GTr
Cuts at: 493 1204 4833 493
Size: 711 3629 2702
Fragments arranged by size:
3629 2702 711
BsaBI GATnn'nnATC
Cuts at: 7036 7036
Size: 7042
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 4221 4849 5231
Size: 53 83 186 936 503 2185 628 382
Cuts at: 5231 5866 275
Size: 635 1451
Fragments arranged by size:
2185 1451 936 635 628 503 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5475 5873 6147 6294 6705 5475
Size: 398 274 147 411 5812
Fragments arranged by size:
5812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6817
Fragments arranged by size:
6817 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 3001 1186
Size: 880 935 5227
Fragments arranged by size:
5227 935 880
BseYI C'CCAG_C
Cuts at: 6245 6741 6245
Size: 496 6546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3516 1163
Size: 573 1780 4689
Fragments arranged by size:
4689 1780 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4492 5253 6281 1540
Size: 275 186 2491 761 1028 2301
Fragments arranged by size:
2491 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3709 5103 5188 6259 3709
Size: 1394 85 1071 4492
Fragments arranged by size:
4492 1394 1071 85
BsmI GAATG_Cn'
Cuts at: 2663 4553 4646 2663
Size: 1890 93 5059
Fragments arranged by size:
5059 1890 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3709 4279 4361 5103 5188 6259 1391
Size: 399 1919 570 82 742 85 1071 2174
Fragments arranged by size:
2174 1919 1071 742 570 399 85 82
BspEI T'CCGG_A
Cuts at: 6705 6705
Size: 7042
BspHI T'CATG_A
Cuts at: 3979 4015 4931 6661 3979
Size: 36 916 1730 4360
Fragments arranged by size:
4360 1730 916 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4397 844
Size: 3553 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 2086 4929 1394
Size: 429 239 24 2843 3507
Fragments arranged by size:
3507 2843 429 239 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 3558 5537 5711 65
Size: 1183 2310 1979 174 1396
Fragments arranged by size:
2310 1979 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5686 5873 1700
Size: 110 3876 187 2869
Fragments arranged by size:
3876 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7042
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7042
BssSI C'ACGA_G
Cuts at: 2745 5102 6114 2745
Size: 2357 1012 3673
Fragments arranged by size:
3673 2357 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7042
BstBI TT'CG_AA
Cuts at: 5846 5846
Size: 7042
BstEII G'GTnAC_C
Cuts at: 1184 4027 1184
Size: 2843 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 3717 3717
Size: 7042
BstYI r'GATC_y
Cuts at: 1972 2384 3225 3361 3529 3905 4372 4739 5124
Size: 412 841 136 168 376 467 367 385
Cuts at: 5124 5141 6593 6679 6691 7037 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 841 467 412 385 376 367
346 168 136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 4762
Fragments arranged by size:
4762 1728 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 487
Size: 683 56 863 5440
Fragments arranged by size:
5440 863 683 56
BtsI GCAGTG_nn'
Cuts at: 4135 4558 5352 5372 4135
Size: 423 794 20 5805
Fragments arranged by size:
5805 794 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7042
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3682 3717 529
Size: 35 3118 35 3854
Fragments arranged by size:
3854 3118 35 35
DraI TTT'AAA
Cuts at: 2616 4346 2616
Size: 1730 5312
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6049 809
Size: 507 4733 1802
Fragments arranged by size:
4733 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2845 3187 3433
Size: 54 1425 50 275 18 1015 342 246
Cuts at: 3433 4489 5378 7007 8
Size: 1056 889 1629 43
Fragments arranged by size:
1629 1425 1056 1015 889 342 275 246
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4489 1537
Size: 275 2677 4090
Fragments arranged by size:
4090 2677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2112 4972 1353
Size: 723 36 2860 3423
Fragments arranged by size:
3423 2860 723 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5629 6003 6149 387
Size: 1064 138 4040 374 146 1280
Fragments arranged by size:
4040 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3707 3707
Size: 7042
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2382 2841 3164 3192 3666 3693
Size: 393 428 322 459 323 28 474 27
Cuts at: 3693 5105 5702 6489 6757 1239
Size: 1412 597 787 268 1524
Fragments arranged by size:
1524 1412 787 597 474 459 428 393
323 322 268 28 27
EcoO109I rG'GnC_Cy
Cuts at: 3842 4305 3842
Size: 463 6579
EcoRI G'AATT_C
Cuts at: 4351 4729 4351
Size: 378 6664
EcoRV GAT'ATC
Cuts at: 2554 2800 2554
Size: 246 6796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3797 3797
Size: 7042
FspI TGC'GCA
Cuts at: 5548 5548
Size: 7042
HaeII r_GCGC'y
Cuts at: 1678 2039 6189 1678
Size: 361 4150 2531
Fragments arranged by size:
4150 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2962 2994 3777 3809 4196
Size: 32 135 32 1390 32 783 32 387
Cuts at: 4196 4228 4241 4273 5683 5715 5757 5789 1373
Size: 32 13 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1410 1390 783 387 135 42 32
32 32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4386 4632 669
Size: 988 281 2448 246 3079
Fragments arranged by size:
3079 2448 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3800 748
Size: 3052 3990
HpaI GTT'AAC
Cuts at: 4632 4632
Size: 7042
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 7042
KpnI G_GTAC'C
Cuts at: 4367 4367
Size: 7042
MfeI C'AATT_G
Cuts at: 4641 4641
Size: 7042
MluI A'CGCG_T
Cuts at: 6918 6918
Size: 7042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2180 3114 6156 6340 1305
Size: 875 934 3042 184 2007
Fragments arranged by size:
3042 2007 934 875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2847 3435 10
Size: 54 1425 343 1015 588 3617
Fragments arranged by size:
3617 1425 1015 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2808 5000 5359 5518 518
Size: 648 1642 2192 359 159 2042
Fragments arranged by size:
2192 2042 1642 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4458 5135 6283 6528 1606
Size: 2852 677 1148 245 2120
Fragments arranged by size:
2852 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 7042
NcoI C'CATG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 4762
Fragments arranged by size:
4762 1728 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7042
NotI GC'GGCC_GC
Cuts at: 4489 4489
Size: 7042
NspI r_CATG'y
Cuts at: 1132 1229 3495 4400 5945 1132
Size: 97 2266 905 1545 2229
Fragments arranged by size:
2266 2229 1545 905 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7042
PciI A'CATG_T
Cuts at: 3491 5941 3491
Size: 2450 4592
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3011 1108
Size: 1903 5139
PfoI T'CCnGG_A
Cuts at: 2076 3141 2076
Size: 1065 5977
PmeI GTTT'AAAC
Cuts at: 4346 4346
Size: 7042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 6624 6656 5111
Size: 32 1481 32 5497
Fragments arranged by size:
5497 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4305 4305
Size: 7042
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7042
PsiI TTA'TAA
Cuts at: 4612 4612
Size: 7042
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2530 2562 2530
Size: 32 7010
PstI C_TGCA'G
Cuts at: 830 4394 830
Size: 3564 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7042
SacI G_AGCT'C
Cuts at: 3709 3709
Size: 7042
SalI G'TCGA_C
Cuts at: 1936 4384 1936
Size: 2448 4594
SapI GCTCTTCn'nnn_
Cuts at: 2076 2076
Size: 7042
SbfI CC_TGCA'GG
Cuts at: 4394 4394
Size: 7042
ScaI AGT'ACT
Cuts at: 3858 5290 7002 3858
Size: 1432 1712 3898
Fragments arranged by size:
3898 1712 1432
SfcI C'TryA_G
Cuts at: 826 1045 2547 3229 3303 4390 5525 6206 6397
Size: 219 1502 682 74 1087 1135 681 191
Cuts at: 6397 826
Size: 1471
Fragments arranged by size:
1502 1471 1135 1087 682 681 219 191
74
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 7042
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7042
SmaI CCC'GGG
Cuts at: 1872 4369 1872
Size: 2497 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4286 5146 6047 6309 820
Size: 197 926 2343 860 901 262 1553
Fragments arranged by size:
2343 1553 926 901 860 262 197
SnaBI TAC'GTA
Cuts at: 493 4833 493
Size: 4340 2702
SpeI A'CTAG_T
Cuts at: 152 3290 152
Size: 3138 3904
SphI G_CATG'C
Cuts at: 1132 4400 1132
Size: 3268 3774
SspI AAT'ATT
Cuts at: 5 52 2685 2949 3043 4966 5
Size: 47 2633 264 94 1923 2081
Fragments arranged by size:
2633 2081 1923 264 94 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7042
StyI C'CwwG_G
Cuts at: 513 1065 2039 2793 4481 513
Size: 552 974 754 1688 3074
Fragments arranged by size:
3074 1688 974 754 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5069 5086 5239 5424 5869 325
Size: 1255 3489 17 153 185 445 1498
Fragments arranged by size:
3489 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3856 5288 7000
Size: 274 80 33 51 1443 1879 1432 1712
Cuts at: 7000 96
Size: 138
Fragments arranged by size:
1879 1712 1443 1432 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7002 7034 7002
Size: 32 7010
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7042
XbaI T'CTAG_A
Cuts at: 4378 4378
Size: 7042
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3130 3130
Size: 7042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7042
XmaI C'CCGG_G
Cuts at: 1870 4367 1870
Size: 2497 4545
XmnI GAAnn'nnTTC
Cuts at: 5171 5171
Size: 7042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4222 4850 276
Size: 53 83 186 936 2688 628 2468
Fragments arranged by size:
2688 2468 936 628 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII
RsrII SacII SanDI SexAI SfiI SrfI SwaI