Restriction map for
pFN21AB3013
(Circular) (Six-base) MAPSORT of: pFN21AB3013.seq Check: 7896 from: 1 to: 6073
pFN21AB3013 6073 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2770 3222 3416 1937
Size: 833 452 194 4594
Fragments arranged by size:
4594 833 452 194
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2825 4136 5520 5788 1632
Size: 1193 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1193 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 2803 3148 3298 3816 5388 2803
Size: 345 150 518 1572 3488
Fragments arranged by size:
3488 1572 518 345 150
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 2555 2639 2898 3382 3608 3760 5869 1493
Size: 1062 84 259 484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 484 259 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 2375 3308 4627 160
Size: 872 1343 933 1319 1606
Fragments arranged by size:
1606 1343 1319 933 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 3398 1870
Size: 73 147 1308 4545
Fragments arranged by size:
4545 1308 147 73
BamHI G'GATC_C
Cuts at: 2717 3262 3403 3770 2717
Size: 545 141 367 5020
Fragments arranged by size:
5020 545 367 141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3394 4849 618
Size: 325 217 627 1607 1455 1842
Fragments arranged by size:
1842 1607 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3392 1391
Size: 2001 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2125 928
Size: 971 226 4876
Fragments arranged by size:
4876 971 226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4684 136
Size: 107 122 71 631 605 3012 1525
Fragments arranged by size:
3012 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 3490 3490
Size: 6073
Bme1580I G_kGCm'C
Cuts at: 1790 4134 5290 1790
Size: 2344 1156 2573
Fragments arranged by size:
2573 2344 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3804 4762 442
Size: 416 53 83 399 24 2387 958 1753
Fragments arranged by size:
2387 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2108 1056
Size: 1052 5021
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4733 1239
Size: 3494 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 4198 5063 5361 3023
Size: 1175 865 298 3735
Fragments arranged by size:
3735 1175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3247 3294 4736 882
Size: 617 1748 47 1442 2219
Fragments arranged by size:
2219 1748 1442 617 47
BsaAI yAC'GTr
Cuts at: 493 1204 3864 493
Size: 711 2660 2702
Fragments arranged by size:
2702 2660 711
BsaBI GATnn'nnATC
Cuts at: 6067 6067
Size: 6073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4506 4904 5178 5325 5736 4506
Size: 398 274 147 411 4843
Fragments arranged by size:
4843 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5848
Fragments arranged by size:
5848 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3365 1186
Size: 2179 3894
BseYI C'CCAG_C
Cuts at: 5276 5772 5276
Size: 496 5577
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4134 4219 5290 4134
Size: 85 1071 4917
Fragments arranged by size:
4917 1071 85
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3392 4134 4219 5290 1391
Size: 399 1602 742 85 1071 2174
Fragments arranged by size:
2174 1602 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 3960 1394
Size: 429 203 1934 3507
Fragments arranged by size:
3507 1934 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 4568 4742 65
Size: 1183 3320 174 1396
Fragments arranged by size:
3320 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 2605 4877 2605
Size: 2272 3801
BstEII G'GTnAC_C
Cuts at: 1184 2395 1184
Size: 1211 4862
BstYI r'GATC_y
Cuts at: 1972 2717 2914 3151 3262 3403 3770 4155 4172
Size: 745 197 237 111 141 367 385 17
Cuts at: 4172 5624 5710 5722 6068 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 745 385 367 346 237 197
141 111 86 17 12
BstZ17I GTA'TAC
Cuts at: 2771 2771
Size: 6073
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 4516
Fragments arranged by size:
4516 939 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5334
Fragments arranged by size:
5334 683 56
BtsI GCAGTG_nn'
Cuts at: 3589 4383 4403 3589
Size: 794 20 5259
Fragments arranged by size:
5259 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2947 2982 529
Size: 35 2383 35 3620
Fragments arranged by size:
3620 2383 35 35
DraI TTT'AAA
Cuts at: 2922 3377 2922
Size: 455 5618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3102 3520 4409
Size: 54 1425 50 275 18 1272 418 889
Cuts at: 4409 6038 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1272 889 418 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 4003 1353
Size: 1448 1202 3423
Fragments arranged by size:
3423 1448 1202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2299 4660 5034 5180 387
Size: 1064 138 435 275 2361 374 146 1280
Fragments arranged by size:
2361 1280 1064 435 374 275 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2825 4136 4733 5520 5788 1239
Size: 393 1193 1311 597 787 268 1524
Fragments arranged by size:
1524 1311 1193 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2138 3017 3226 2138
Size: 879 209 4985
Fragments arranged by size:
4985 879 209
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 2070 2086 5220 1678
Size: 392 16 3134 2531
Fragments arranged by size:
3134 2531 392 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2171 2203 3349
Size: 32 135 32 431 32 136 32 1146
Cuts at: 3349 3381 4714 4746 4788 4820 1373
Size: 32 1333 32 42 32 2626
Fragments arranged by size:
2626 1333 1146 431 136 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3199 3322 3417 3663 669
Size: 988 281 1261 123 95 246 3079
Fragments arranged by size:
3079 1261 988 281 246 123 95
HindIII A'AGCT_T
Cuts at: 748 2924 748
Size: 2176 3897
HpaI GTT'AAC
Cuts at: 3199 3322 3663 3199
Size: 123 341 5609
Fragments arranged by size:
5609 341 123
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5187 5371 1305
Size: 3882 184 2007
Fragments arranged by size:
3882 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3104 10
Size: 54 1425 343 1272 2979
Fragments arranged by size:
2979 1425 1272 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4031 4390 4549 518
Size: 648 2865 359 159 2042
Fragments arranged by size:
2865 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2701 3489 4166 5314 5559 1606
Size: 1095 788 677 1148 245 2120
Fragments arranged by size:
2120 1148 1095 788 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4582
Fragments arranged by size:
4582 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 2104 1052
Size: 1052 5021
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 3431 4976 1132
Size: 97 2202 1545 2229
Fragments arranged by size:
2229 2202 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6073
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6073
PfoI T'CCnGG_A
Cuts at: 2646 2646
Size: 6073
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PstI C_TGCA'G
Cuts at: 830 3425 830
Size: 2595 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2701 2701
Size: 6073
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 2681 4321 6033 2681
Size: 1640 1712 2721
Fragments arranged by size:
2721 1712 1640
SfcI C'TryA_G
Cuts at: 826 1045 2062 2743 3033 3421 4556 5237 5428
Size: 219 1017 681 290 388 1135 681 191
Cuts at: 5428 826
Size: 1471
Fragments arranged by size:
1471 1135 1017 681 681 388 290 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3400 1872
Size: 1528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3002 4177 5078 5340 820
Size: 197 926 1059 1175 901 262 1553
Fragments arranged by size:
1553 1175 1059 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 2263 3091 3997 5
Size: 47 2211 828 906 2081
Fragments arranged by size:
2211 2081 906 828 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 2004 2109 3258 3512 513
Size: 552 939 105 1149 254 3074
Fragments arranged by size:
3074 1149 939 552 254 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4100 4117 4270 4455 4900 325
Size: 1255 2520 17 153 185 445 1498
Fragments arranged by size:
2520 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2330 2679 2815
Size: 274 80 33 51 1443 353 349 136
Cuts at: 2815 4319 6031 96
Size: 1504 1712 138
Fragments arranged by size:
1712 1504 1443 353 349 274 138 136
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2373 6033 6065 2341
Size: 32 3660 32 2349
Fragments arranged by size:
3660 2349 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 3398 1870
Size: 1528 4545
XmnI GAAnn'nnTTC
Cuts at: 2142 2898 2995 3177 4202 2142
Size: 756 97 182 1025 4013
Fragments arranged by size:
4013 1025 756 182 97
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI