Restriction map for
pFN21AB3011
(Circular) (Six-base) MAPSORT of: pFN21AB3011.seq Check: 6054 from: 1 to: 6868
pFN21AB3011 6868 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2693 2693
Size: 6868
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4678 278
Size: 53 83 186 936 3142 2468
Fragments arranged by size:
3142 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2118 4211 1937
Size: 181 2093 4594
Fragments arranged by size:
4594 2093 181
Acc65I G'GTAC_C
Cuts at: 4189 4189
Size: 6868
AclI AA'CG_TT
Cuts at: 1563 4167 4995 5368 1563
Size: 2604 828 373 3063
Fragments arranged by size:
3063 2604 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2592 4931 6315 6583 1632
Size: 893 67 2339 1384 268 1917
Fragments arranged by size:
2339 1917 1384 893 268 67
AfeI AGC'GCT
Cuts at: 3522 3522
Size: 6868
AflII C'TTAA_G
Cuts at: 820 1017 4073 820
Size: 197 3056 3615
Fragments arranged by size:
3615 3056 197
AflIII A'CryG_T
Cuts at: 1203 2646 5767 6744 1203
Size: 1443 3121 977 1327
Fragments arranged by size:
3121 1443 1327 977
AgeI A'CCGG_T
Cuts at: 5699 5699
Size: 6868
AhdI GACnn_n'nnGTC
Cuts at: 5597 5597
Size: 6868
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6868
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6868
AlwNI CAG_nnn'CTG
Cuts at: 3544 4611 6183 3544
Size: 1067 1572 4229
Fragments arranged by size:
4229 1572 1067
ApaI G_GGCC'C
Cuts at: 2372 2372
Size: 6868
ApaLI G'TGCA_C
Cuts at: 2706 4925 6081 2706
Size: 2219 1156 3493
Fragments arranged by size:
3493 2219 1156
ApoI r'AATT_y
Cuts at: 1493 2015 2889 4125 4177 4403 4555 6664 1493
Size: 522 874 1236 52 226 152 2109 1697
Fragments arranged by size:
2109 1697 1236 874 522 226 152 52
AseI AT'TA_AT
Cuts at: 160 1032 2484 5422 160
Size: 872 1452 2938 1606
Fragments arranged by size:
2938 1606 1452 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
AvaI C'yCGr_G
Cuts at: 1870 1943 4025 4193 1870
Size: 73 2082 168 4545
Fragments arranged by size:
4545 2082 168 73
BamHI G'GATC_C
Cuts at: 2539 3238 4198 4565 2539
Size: 699 960 367 4842
Fragments arranged by size:
4842 960 699 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4189 5644 618
Size: 325 217 627 2402 1455 1842
Fragments arranged by size:
2402 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2294 2372 2424 3157 4187 1391
Size: 903 78 52 733 1030 4072
Fragments arranged by size:
4072 1030 903 733 78 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 2499 3768 928
Size: 971 322 278 1269 4028
Fragments arranged by size:
4028 1269 971 322 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5059 5093 1903
Size: 34 3122 34 3678
Fragments arranged by size:
3678 3122 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4760 5970 4760
Size: 1210 5658
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6868
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3277 5479 136
Size: 107 122 71 631 605 1605 2202 1525
Fragments arranged by size:
2202 1605 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2458 2597 3427 6863 2458
Size: 139 830 3436 2463
Fragments arranged by size:
3436 2463 830 139
BlpI GC'TnA_GC
Cuts at: 4285 4285
Size: 6868
Bme1580I G_kGCm'C
Cuts at: 1790 2372 2710 4929 6085 1790
Size: 582 338 2219 1156 2573
Fragments arranged by size:
2573 2219 1156 582 338
BmgBI CAC'GTC
Cuts at: 1318 2606 1318
Size: 1288 5580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 3073 4599
Size: 416 53 83 399 24 839 817 1526
Cuts at: 4599 5557 442
Size: 958 1753
Fragments arranged by size:
1753 1526 958 839 817 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 3254 3338 5528 1239
Size: 1142 873 84 2190 2579
Fragments arranged by size:
2579 2190 1142 873 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 4993 5858 6156 2408
Size: 2585 865 298 3120
Fragments arranged by size:
3120 2585 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5531 882
Size: 617 4032 2219
Fragments arranged by size:
4032 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2195 4659 493
Size: 711 991 2464 2702
Fragments arranged by size:
2702 2464 991 711
BsaBI GATnn'nnATC
Cuts at: 3216 6862 3216
Size: 3646 3222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4675 5057 5692 275
Size: 53 83 186 936 3142 382 635 1451
Fragments arranged by size:
3142 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5301 5699 5973 6120 6531 5301
Size: 398 274 147 411 5638
Fragments arranged by size:
5638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3373 3403 1178
Size: 30 165 30 1970 30 4643
Fragments arranged by size:
4643 1970 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6868
BseYI C'CCAG_C
Cuts at: 3621 6071 6567 3621
Size: 2450 496 3922
Fragments arranged by size:
3922 2450 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 1163
Size: 573 699 5596
Fragments arranged by size:
5596 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4318 5079 6107 1540
Size: 275 186 2317 761 1028 2301
Fragments arranged by size:
2317 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2710 3157 4929 5014 6085 2710
Size: 447 1772 85 1071 3493
Fragments arranged by size:
3493 1772 1071 447 85
BsmI GAATG_Cn'
Cuts at: 4149 4379 4472 4149
Size: 230 93 6545
Fragments arranged by size:
6545 230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2372 2424 2710 3157 4187 4929
Size: 399 504 78 52 286 447 1030 742
Cuts at: 4929 5014 6085 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1030 742 504 447 399 286
85 78 52
BspEI T'CCGG_A
Cuts at: 6531 6531
Size: 6868
BspHI T'CATG_A
Cuts at: 2028 3076 3295 4757 6487 2028
Size: 1048 219 1462 1730 2409
Fragments arranged by size:
2409 1730 1462 1048 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 4223 844
Size: 1849 1530 3489
Fragments arranged by size:
3489 1849 1530
BsrBI CCG'CTC
Cuts at: 1394 1823 4755 1394
Size: 429 2932 3507
Fragments arranged by size:
3507 2932 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2101 5363 5537 65
Size: 1183 853 3262 174 1396
Fragments arranged by size:
3262 1396 1183 853 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5512 5699 1700
Size: 110 3702 187 2869
Fragments arranged by size:
3702 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2755 3109 96
Size: 2659 354 3855
Fragments arranged by size:
3855 2659 354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6868
BssSI C'ACGA_G
Cuts at: 4928 5940 4928
Size: 1012 5856
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6868
BstBI TT'CG_AA
Cuts at: 5672 5672
Size: 6868
BstEII G'GTnAC_C
Cuts at: 1184 4065 1184
Size: 2881 3987
BstYI r'GATC_y
Cuts at: 1972 2458 2539 2597 3238 3427 4198 4565 4950
Size: 486 81 58 641 189 771 367 385
Cuts at: 4950 4967 6419 6505 6517 6863 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 771 641 486 385 367 346
189 86 81 58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2907 513
Size: 552 939 903 4474
Fragments arranged by size:
4474 939 903 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6129
Fragments arranged by size:
6129 683 56
BtsI GCAGTG_nn'
Cuts at: 4384 5178 5198 4384
Size: 794 20 6054
Fragments arranged by size:
6054 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6868
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6833
DraI TTT'AAA
Cuts at: 4172 4172
Size: 6868
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5875 809
Size: 507 4559 1802
Fragments arranged by size:
4559 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4315 5204 6833
Size: 54 1425 50 275 18 2485 889 1629
Cuts at: 6833 8
Size: 43
Fragments arranged by size:
2485 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4315 1537
Size: 275 2503 4090
Fragments arranged by size:
4090 2503 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 4798 1353
Size: 1023 2422 3423
Fragments arranged by size:
3423 2422 1023
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5455 5829 5975 387
Size: 1064 138 3866 374 146 1280
Fragments arranged by size:
3866 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3155 3155
Size: 6868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2525 2592 3254 3338 4931 5528
Size: 393 749 144 67 662 84 1593 597
Cuts at: 5528 6315 6583 1239
Size: 787 268 1524
Fragments arranged by size:
1593 1524 787 749 662 597 393 268
144 84 67
EcoO109I rG'GnC_Cy
Cuts at: 2368 2369 2368
Size: 1 6867
EcoRI G'AATT_C
Cuts at: 4177 4555 4177
Size: 378 6490
EcoRV GAT'ATC
Cuts at: 3702 3702
Size: 6868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3012 3158 3012
Size: 146 6722
FspI TGC'GCA
Cuts at: 5374 5374
Size: 6868
HaeII r_GCGC'y
Cuts at: 1678 3524 6015 1678
Size: 1846 2491 2531
Fragments arranged by size:
2531 2491 1846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2130 2162 2242 2274 2276
Size: 32 135 32 558 32 80 32 2
Cuts at: 2276 2308 2524 2556 3518 3550 5509 5541 5583
Size: 32 216 32 962 32 1959 32 42
Cuts at: 5583 5615 1373
Size: 32 2626
Fragments arranged by size:
2626 1959 962 558 216 135 80 42
32 32 32 32 32 32 32 32
32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3397 4212 4458 669
Size: 988 281 981 478 815 246 3079
Fragments arranged by size:
3079 988 981 815 478 281 246
HindIII A'AGCT_T
Cuts at: 748 3416 3956 748
Size: 2668 540 3660
Fragments arranged by size:
3660 2668 540
HpaI GTT'AAC
Cuts at: 2919 3397 4458 2919
Size: 478 1061 5329
Fragments arranged by size:
5329 1061 478
KpnI G_GTAC'C
Cuts at: 4193 4193
Size: 6868
MfeI C'AATT_G
Cuts at: 3436 4467 3436
Size: 1031 5837
MluI A'CGCG_T
Cuts at: 6744 6744
Size: 6868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5982 6166 1305
Size: 4677 184 2007
Fragments arranged by size:
4677 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5046
Fragments arranged by size:
5046 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 3405 4826 5185 5344 518
Size: 648 1479 760 1421 359 159 2042
Fragments arranged by size:
2042 1479 1421 760 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2410 2731 2845 4284 4961 6109 6354 1606
Size: 804 321 114 1439 677 1148 245 2120
Fragments arranged by size:
2120 1439 1148 804 677 321 245 114
NcoI C'CATG_G
Cuts at: 513 1065 2004 2907 513
Size: 552 939 903 4474
Fragments arranged by size:
4474 939 903 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6868
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6868
NotI GC'GGCC_GC
Cuts at: 4315 4315
Size: 6868
NspI r_CATG'y
Cuts at: 1132 1229 2189 2390 2650 4226 5771 1132
Size: 97 960 201 260 1576 1545 2229
Fragments arranged by size:
2229 1576 1545 960 260 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6868
PciI A'CATG_T
Cuts at: 2646 5767 2646
Size: 3121 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6868
PfoI T'CCnGG_A
Cuts at: 2851 2880 2851
Size: 29 6839
PmeI GTTT'AAAC
Cuts at: 4172 4172
Size: 6868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4937 4969 6450 6482 4937
Size: 32 1481 32 5323
Fragments arranged by size:
5323 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2520 1613
Size: 907 5961
PsiI TTA'TAA
Cuts at: 3060 4438 3060
Size: 1378 5490
PspOMI G'GGCC_C
Cuts at: 2368 2368
Size: 6868
PspXI vC'TCGA_Gb
Cuts at: 4025 4025
Size: 6868
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2300 2332 2300
Size: 32 6836
PstI C_TGCA'G
Cuts at: 830 2659 4220 830
Size: 1829 1561 3478
Fragments arranged by size:
3478 1829 1561
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6868
PvuII CAG'CTG
Cuts at: 2410 2731 2845 2410
Size: 321 114 6433
Fragments arranged by size:
6433 321 114
SacI G_AGCT'C
Cuts at: 3157 3157
Size: 6868
SalI G'TCGA_C
Cuts at: 1936 4210 1936
Size: 2274 4594
SapI GCTCTTCn'nnn_
Cuts at: 2376 2376
Size: 6868
SbfI CC_TGCA'GG
Cuts at: 4220 4220
Size: 6868
ScaI AGT'ACT
Cuts at: 5116 6828 5116
Size: 1712 5156
SfcI C'TryA_G
Cuts at: 826 1045 2115 2655 4216 5351 6032 6223 826
Size: 219 1070 540 1561 1135 681 191 1471
Fragments arranged by size:
1561 1471 1135 1070 681 540 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6868
SmaI CCC'GGG
Cuts at: 1872 4195 1872
Size: 2323 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 4025 4073 4972 5873 6135
Size: 197 926 480 1602 48 899 901 262
Cuts at: 6135 820
Size: 1553
Fragments arranged by size:
1602 1553 926 901 899 480 262 197
48
SnaBI TAC'GTA
Cuts at: 493 4659 493
Size: 4166 2702
SpeI A'CTAG_T
Cuts at: 152 3881 152
Size: 3729 3139
SphI G_CATG'C
Cuts at: 1132 2390 4226 1132
Size: 1258 1836 3774
Fragments arranged by size:
3774 1836 1258
SspI AAT'ATT
Cuts at: 5 52 3206 3308 4792 5
Size: 47 3154 102 1484 2081
Fragments arranged by size:
3154 2081 1484 102 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6868
StyI C'CwwG_G
Cuts at: 513 1065 2004 2907 4307 513
Size: 552 939 903 1400 3074
Fragments arranged by size:
3074 1400 939 903 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4895 4912 5065 5250 5695 325
Size: 1255 3315 17 153 185 445 1498
Fragments arranged by size:
3315 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 2446 2755
Size: 274 80 33 51 1443 354 115 309
Cuts at: 2755 3109 3184 5114 6826 96
Size: 354 75 1930 1712 138
Fragments arranged by size:
1930 1712 1443 354 354 309 274 138
115 80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2862 2894 3373 3405 6828 6860 2862
Size: 32 479 32 3423 32 2870
Fragments arranged by size:
3423 2870 479 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6868
XbaI T'CTAG_A
Cuts at: 4204 4204
Size: 6868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3797 3797
Size: 6868
XhoI C'TCGA_G
Cuts at: 1943 4025 1943
Size: 2082 4786
XmaI C'CCGG_G
Cuts at: 1870 4193 1870
Size: 2323 4545
XmnI GAAnn'nnTTC
Cuts at: 2745 3984 4997 2745
Size: 1239 1013 4616
Fragments arranged by size:
4616 1239 1013
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4676 276
Size: 53 83 186 936 3142 2468
Fragments arranged by size:
3142 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI