Restriction map for
pFN21AB3009
(Circular) (Six-base) MAPSORT of: pFN21AB3009.seq Check: 5388 from: 1 to: 6649
pFN21AB3009 6649 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4459 278
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3094 3992 1937
Size: 1157 898 4594
Fragments arranged by size:
4594 1157 898
Acc65I G'GTAC_C
Cuts at: 3657 3970 3657
Size: 313 6336
AclI AA'CG_TT
Cuts at: 1563 2717 4776 5149 1563
Size: 1154 2059 373 3063
Fragments arranged by size:
3063 2059 1154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3468 3541 3625 3793 3877 4712 6096 6364
Size: 1836 73 84 168 84 835 1384 268
Cuts at: 6364 1632
Size: 1917
Fragments arranged by size:
1917 1836 1384 835 268 168 84 84
73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 5548 6525 1203
Size: 4345 977 1327
Fragments arranged by size:
4345 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 5378 5378
Size: 6649
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6649
AlwNI CAG_nnn'CTG
Cuts at: 2102 2227 2605 2710 4392 5964 2102
Size: 125 378 105 1682 1572 2787
Fragments arranged by size:
2787 1682 1572 378 125 105
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 2055 3045 3385 3958 4184 4336 6445 1493
Size: 562 990 340 573 226 152 2109 1697
Fragments arranged by size:
2109 1697 990 573 562 340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5203 160
Size: 872 4171 1606
Fragments arranged by size:
4171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 2516 2630 3974 1870
Size: 73 573 114 1344 4545
Fragments arranged by size:
4545 1344 573 114 73
BamHI G'GATC_C
Cuts at: 2494 3979 4346 2494
Size: 1485 367 4797
Fragments arranged by size:
4797 1485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2560 3330 3657 3970 5425
Size: 325 217 627 773 770 327 313 1455
Cuts at: 5425 618
Size: 1842
Fragments arranged by size:
1842 1455 773 770 627 327 325 313
217
BanII G_rGCy'C
Cuts at: 1391 2549 2598 2663 3968 1391
Size: 1158 49 65 1305 4072
Fragments arranged by size:
4072 1305 1158 65 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2817 3680 928
Size: 971 918 863 3897
Fragments arranged by size:
3897 971 918 863
BbvCI CC'TCA_GC
Cuts at: 3687 3687
Size: 6649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4840 4874 1903
Size: 34 2903 34 3678
Fragments arranged by size:
3678 2903 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4541 5751 4541
Size: 1210 5439
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5260 136
Size: 107 122 71 631 605 3588 1525
Fragments arranged by size:
3588 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2551 2862 2998 6644 2551
Size: 311 136 3646 2556
Fragments arranged by size:
3646 2556 311 136
BlpI GC'TnA_GC
Cuts at: 4066 4066
Size: 6649
Bme1580I G_kGCm'C
Cuts at: 1790 2563 2631 3875 4710 5866 1790
Size: 773 68 1244 835 1156 2573
Fragments arranged by size:
2573 1244 1156 835 773 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6649
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 3861 4380
Size: 416 53 83 399 24 1201 1243 519
Cuts at: 4380 5338 442
Size: 958 1753
Fragments arranged by size:
1753 1243 1201 958 519 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2677 2210
Size: 467 6182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 2582 2685 3008 3042 3537 5309 1239
Size: 1083 260 103 323 34 495 1772 2579
Fragments arranged by size:
2579 1772 1083 495 323 260 103 34
Bpu10I CC'TnA_GC
Cuts at: 3687 3687
Size: 6649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2907 3114 3775 4774 5639 5937 2907
Size: 207 661 999 865 298 3619
Fragments arranged by size:
3619 999 865 661 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2224 2386 3389 5312 882
Size: 617 725 162 1003 1923 2219
Fragments arranged by size:
2219 1923 1003 725 617 162
BsaAI yAC'GTr
Cuts at: 493 1204 3870 4440 493
Size: 711 2666 570 2702
Fragments arranged by size:
2702 2666 711 570
BsaBI GATnn'nnATC
Cuts at: 6643 6643
Size: 6649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4456 4838 5473 275
Size: 53 83 186 936 2923 382 635 1451
Fragments arranged by size:
2923 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3378 3669 5082 5480 5754 5901 6312 3378
Size: 291 1413 398 274 147 411 3715
Fragments arranged by size:
3715 1413 411 398 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2459 2489 2784 2814 1178
Size: 30 165 30 1056 30 295 30 5013
Fragments arranged by size:
5013 1056 295 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2459 2606 2671 1186
Size: 1177 96 147 65 5164
Fragments arranged by size:
5164 1177 147 96 65
BseYI C'CCAG_C
Cuts at: 2316 5852 6348 2316
Size: 3536 496 2617
Fragments arranged by size:
3536 2617 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6076
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4099 4860 5888 1540
Size: 275 186 2098 761 1028 2301
Fragments arranged by size:
2301 2098 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 2663 4710 4795 5866 2598
Size: 65 2047 85 1071 3381
Fragments arranged by size:
3381 2047 1071 85 65
BsmI GAATG_Cn'
Cuts at: 4160 4253 4160
Size: 93 6556
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 6649
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2549 2563 2598 2631 2663 3875 3968
Size: 399 759 14 35 33 32 1212 93
Cuts at: 3968 4710 4795 5866 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1212 1071 759 742 399 93 85
35 33 32 14
BspEI T'CCGG_A
Cuts at: 6312 6312
Size: 6649
BspHI T'CATG_A
Cuts at: 3193 4538 6268 3193
Size: 1345 1730 3574
Fragments arranged by size:
3574 1730 1345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4004 844
Size: 3160 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2465 4536 1394
Size: 429 642 2071 3507
Fragments arranged by size:
3507 2071 642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5144 5318 65
Size: 1183 3896 174 1396
Fragments arranged by size:
3896 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2563 5293 5480 1700
Size: 110 753 2730 187 2869
Fragments arranged by size:
2869 2730 753 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 4709 5721 4709
Size: 1012 5637
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3711 794
Size: 2917 3732
BstBI TT'CG_AA
Cuts at: 5453 5453
Size: 6649
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6649
BstXI CCAn_nnnn'nTGG
Cuts at: 2403 2403
Size: 6649
BstYI r'GATC_y
Cuts at: 1972 2035 2256 2494 2551 2862 2998 3979 4346
Size: 63 221 238 57 311 136 981 367
Cuts at: 4346 4731 4748 6200 6286 6298 6644 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 981 385 367 346 311 238
221 136 86 63 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 2529 2913 3251 2529
Size: 384 338 5927
Fragments arranged by size:
5927 384 338
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 5146
Fragments arranged by size:
5146 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5910
Fragments arranged by size:
5910 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 4165 4959 4979 2361
Size: 1804 794 20 4031
Fragments arranged by size:
4031 1804 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3778 3813 529
Size: 35 3214 35 3365
Fragments arranged by size:
3365 3214 35 35
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3827 5656 809
Size: 507 2511 1829 1802
Fragments arranged by size:
2511 1829 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2093 2333 2425
Size: 54 1425 50 275 18 263 240 92
Cuts at: 2425 2730 4096 4985 6614 8
Size: 305 1366 889 1629 43
Fragments arranged by size:
1629 1425 1366 889 305 275 263 240
92 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 2583 2691 2877 2973 3387 3797 4579
Size: 690 540 108 186 96 414 410 782
Cuts at: 4579 1353
Size: 3423
Fragments arranged by size:
3423 782 690 540 414 410 186 108
96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 5236 5610 5756 387
Size: 1064 138 668 2979 374 146 1280
Fragments arranged by size:
2979 1280 1064 668 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2661 2596
Size: 65 6584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2322 2582 2685 3008 3042 3468 3537
Size: 393 690 260 103 323 34 426 69
Cuts at: 3537 3541 3625 3793 3877 4712 5309 6096 6364
Size: 4 84 168 84 835 597 787 268
Cuts at: 6364 1239
Size: 1524
Fragments arranged by size:
1524 835 787 690 597 426 393 323
268 260 168 103 84 84 69 34
4
EcoNI CCTnn'n_nnAGG
Cuts at: 2441 3772 2441
Size: 1331 5318
EcoO109I rG'GnC_Cy
Cuts at: 2620 2635 2893 3777 3927 2620
Size: 15 258 884 150 5342
Fragments arranged by size:
5342 884 258 150 15
EcoRI G'AATT_C
Cuts at: 2055 3958 4336 2055
Size: 1903 378 4368
Fragments arranged by size:
4368 1903 378
EcoRV GAT'ATC
Cuts at: 2902 3905 2902
Size: 1003 5646
FspI TGC'GCA
Cuts at: 5155 5155
Size: 6649
HaeII r_GCGC'y
Cuts at: 1678 3721 5796 1678
Size: 2043 2075 2531
Fragments arranged by size:
2531 2075 2043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2536 2568 2668
Size: 32 135 32 887 32 45 32 100
Cuts at: 2668 2700 2784 2816 2849 2881 3796 3828 5290
Size: 32 84 32 33 32 915 32 1462
Cuts at: 5290 5322 5364 5396 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1462 915 887 135 100 84 45
42 33 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3993 4239 669
Size: 988 281 1638 417 246 3079
Fragments arranged by size:
3079 1638 988 417 281 246
HindIII A'AGCT_T
Cuts at: 748 3553 3889 748
Size: 2805 336 3508
Fragments arranged by size:
3508 2805 336
HpaI GTT'AAC
Cuts at: 3576 4239 3576
Size: 663 5986
KpnI G_GTAC'C
Cuts at: 3661 3974 3661
Size: 313 6336
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 5763 5947 1305
Size: 871 3587 184 2007
Fragments arranged by size:
3587 2007 871 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4827
Fragments arranged by size:
4827 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3868 4607 4966 5125 518
Size: 648 2702 739 359 159 2042
Fragments arranged by size:
2702 2042 739 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3477 4065 4742 5890 6135 1606
Size: 1871 588 677 1148 245 2120
Fragments arranged by size:
2120 1871 1148 677 588 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5158
Fragments arranged by size:
5158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 4007 5552 1132
Size: 97 2778 1545 2229
Fragments arranged by size:
2778 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6649
PciI A'CATG_T
Cuts at: 5548 5548
Size: 6649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2342 1108
Size: 1234 5415
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PmlI CAC'GTG
Cuts at: 3870 3870
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3684 3716 4718 4750 6231 6263 3684
Size: 32 1002 32 1481 32 4070
Fragments arranged by size:
4070 1481 1002 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2620 2635 2893 3927 2620
Size: 15 258 1034 5342
Fragments arranged by size:
5342 1034 258 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6649
PsiI TTA'TAA
Cuts at: 4219 4219
Size: 6649
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 6617
PstI C_TGCA'G
Cuts at: 830 2493 2610 2653 3707 4001 830
Size: 1663 117 43 1054 294 3478
Fragments arranged by size:
3478 1663 1054 294 117 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
SacI G_AGCT'C
Cuts at: 2598 2663 2598
Size: 65 6584
SalI G'TCGA_C
Cuts at: 1936 3991 1936
Size: 2055 4594
SanDI GG'GwC_CC
Cuts at: 2893 2893
Size: 6649
SapI GCTCTTCn'nnn_
Cuts at: 2691 2877 3797 2691
Size: 186 920 5543
Fragments arranged by size:
5543 920 186
SbfI CC_TGCA'GG
Cuts at: 2610 4001 2610
Size: 1391 5258
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SfcI C'TryA_G
Cuts at: 826 1045 2010 2309 2489 2606 2649 3445 3703
Size: 219 965 299 180 117 43 796 258
Cuts at: 3703 3997 5132 5813 6004 826
Size: 294 1135 681 191 1471
Fragments arranged by size:
1471 1135 965 796 681 299 294 258
219 191 180 117 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 3976 1872
Size: 2104 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2922 3129 3754 4753 5654 5916
Size: 197 926 979 207 625 999 901 262
Cuts at: 5916 820
Size: 1553
Fragments arranged by size:
1553 999 979 926 901 625 262 207
197
SnaBI TAC'GTA
Cuts at: 493 4440 493
Size: 3947 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 4007 1132
Size: 2875 3774
SspI AAT'ATT
Cuts at: 5 52 3124 4573 5
Size: 47 3072 1449 2081
Fragments arranged by size:
3072 2081 1449 47
StuI AGG'CCT
Cuts at: 1517 3025 1517
Size: 1508 5141
StyI C'CwwG_G
Cuts at: 513 1065 2004 2396 2888 4088 513
Size: 552 939 392 492 1200 3074
Fragments arranged by size:
3074 1200 939 552 492 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 2395 3743 4676 4693 4846 5031
Size: 1255 728 87 1348 933 17 153 185
Cuts at: 5031 5476 325
Size: 445 1498
Fragments arranged by size:
1498 1348 1255 933 728 445 185 153
87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3536 4895 6607
Size: 274 80 33 51 1443 1559 1359 1712
Cuts at: 6607 96
Size: 138
Fragments arranged by size:
1712 1559 1443 1359 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6609 6641 6609
Size: 32 6617
Tth111I GACn'n_nGTC
Cuts at: 1316 3491 1316
Size: 2175 4474
XbaI T'CTAG_A
Cuts at: 3985 3985
Size: 6649
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 3974 1870
Size: 2104 4545
XmnI GAAnn'nnTTC
Cuts at: 2283 2783 3806 3863 4778 2283
Size: 500 1023 57 915 4154
Fragments arranged by size:
4154 1023 915 500 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4457 276
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI
PspXI PvuII RsrII SacII SexAI SfiI SfoI SrfI
SwaI XcmI