Restriction map for
pFN21AB3007
(Circular) (Six-base) MAPSORT of: pFN21AB3007.seq Check: 9543 from: 1 to: 8452
pFN21AB3007 8452 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6262 278
Size: 53 83 186 936 4726 2468
Fragments arranged by size:
4726 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 3107 3781 5795 1937
Size: 208 962 674 2014 4594
Fragments arranged by size:
4594 2014 962 674 208
Acc65I G'GTAC_C
Cuts at: 5773 5773
Size: 8452
AclI AA'CG_TT
Cuts at: 1563 6579 6952 1563
Size: 5016 373 3063
Fragments arranged by size:
5016 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3621 3689 3868 4044 4182 5091 6515 7899
Size: 1989 68 179 176 138 909 1424 1384
Cuts at: 7899 8167 1632
Size: 268 1917
Fragments arranged by size:
1989 1917 1424 1384 909 268 179 176
138 68
AflII C'TTAA_G
Cuts at: 820 1017 2294 3814 820
Size: 197 1277 1520 5458
Fragments arranged by size:
5458 1520 1277 197
AflIII A'CryG_T
Cuts at: 1203 2803 3796 7351 8328 1203
Size: 1600 993 3555 977 1327
Fragments arranged by size:
3555 1600 1327 993 977
AgeI A'CCGG_T
Cuts at: 7283 7283
Size: 8452
AhdI GACnn_n'nnGTC
Cuts at: 7181 7181
Size: 8452
AleI CACnn'nnGTG
Cuts at: 1166 3461 1166
Size: 2295 6157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8452
AlwNI CAG_nnn'CTG
Cuts at: 4898 6195 7767 4898
Size: 1297 1572 5583
Fragments arranged by size:
5583 1572 1297
ApaI G_GGCC'C
Cuts at: 3262 3262
Size: 8452
ApaLI G'TGCA_C
Cuts at: 6509 7665 6509
Size: 1156 7296
ApoI r'AATT_y
Cuts at: 1493 2468 2548 2874 3013 3024 3730 3978 4118
Size: 975 80 326 139 11 706 248 140
Cuts at: 4118 4196 4556 5091 5603 5761 5987 6139 8248
Size: 78 360 535 512 158 226 152 2109
Cuts at: 8248 1493
Size: 1697
Fragments arranged by size:
2109 1697 975 706 535 512 360 326
248 226 158 152 140 139 80 78
11
AseI AT'TA_AT
Cuts at: 160 1032 3039 7006 160
Size: 872 2007 3967 1606
Fragments arranged by size:
3967 2007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8452
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 5777 1870
Size: 73 206 3628 4545
Fragments arranged by size:
4545 3628 206 73
AvrII C'CTAG_G
Cuts at: 4177 4177
Size: 8452
BamHI G'GATC_C
Cuts at: 3802 4916 5782 6149 3802
Size: 1114 866 367 6105
Fragments arranged by size:
6105 1114 866 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5773 7228 618
Size: 325 217 627 3986 1455 1842
Fragments arranged by size:
3986 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2158 2794 3262 3448 5771 1391
Size: 640 127 636 468 186 2323 4072
Fragments arranged by size:
4072 2323 640 636 468 186 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3225 3348 5306 928
Size: 971 1326 123 1958 4074
Fragments arranged by size:
4074 1958 1326 971 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6643 6677 1903
Size: 34 4706 34 3678
Fragments arranged by size:
4706 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2382 5739 6344 7554 2382
Size: 3357 605 1210 3280
Fragments arranged by size:
3357 3280 1210 605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8452
BfrBI ATG'CAT
Cuts at: 3368 4301 5570 3368
Size: 933 1269 6250
Fragments arranged by size:
6250 1269 933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7063 136
Size: 107 122 71 631 605 5391 1525
Fragments arranged by size:
5391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4351 5083 8447 4351
Size: 732 3364 4356
Fragments arranged by size:
4356 3364 732
BlpI GC'TnA_GC
Cuts at: 5869 5869
Size: 8452
Bme1580I G_kGCm'C
Cuts at: 1790 3262 5395 6513 7669 1790
Size: 1472 2133 1118 1156 2573
Fragments arranged by size:
2573 2133 1472 1156 1118
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4390 4394 6183
Size: 416 53 83 399 24 2973 4 1789
Cuts at: 6183 7141 442
Size: 958 1753
Fragments arranged by size:
2973 1789 1753 958 416 399 83 53
24 4
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 4667 7112 1239
Size: 1402 2026 2445 2579
Fragments arranged by size:
2579 2445 2026 1402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2481 2677 3477 4777 5409 6577 7442 7740
Size: 118 196 800 1300 632 1168 865 298
Cuts at: 7740 2363
Size: 3075
Fragments arranged by size:
3075 1300 1168 865 800 632 298 196
118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2057 7115 882
Size: 617 552 6 5058 2219
Fragments arranged by size:
5058 2219 617 552 6
BsaAI yAC'GTr
Cuts at: 493 1204 3797 6243 493
Size: 711 2593 2446 2702
Fragments arranged by size:
2702 2593 2446 711
BsaBI GATnn'nnATC
Cuts at: 4620 8446 4620
Size: 3826 4626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6259 6641 7276 275
Size: 53 83 186 936 4726 382 635 1451
Fragments arranged by size:
4726 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2066 3670 6885 7283 7557 7704 8115 2066
Size: 1604 3215 398 274 147 411 2403
Fragments arranged by size:
3215 2403 1604 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 3013 3043 1178
Size: 30 165 30 1350 30 230 30 6587
Fragments arranged by size:
6587 1350 230 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2802 3303 4272 5477 1186
Size: 853 763 501 969 1205 4161
Fragments arranged by size:
4161 1205 969 853 763 501
BseYI C'CCAG_C
Cuts at: 3752 7655 8151 3752
Size: 3903 496 4053
Fragments arranged by size:
4053 3903 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3615 1163
Size: 573 1879 6000
Fragments arranged by size:
6000 1879 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5902 6663 7691 1540
Size: 275 186 3901 761 1028 2301
Fragments arranged by size:
3901 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2794 2872 3448 6513 6598 7669 2121
Size: 673 78 576 3065 85 1071 2904
Fragments arranged by size:
3065 2904 1071 673 576 85 78
BsmI GAATG_Cn'
Cuts at: 5963 6056 5963
Size: 93 8359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2121 2158 2794 2872 3262 3448
Size: 399 241 90 37 636 78 390 186
Cuts at: 3448 5395 5771 6513 6598 7669 1391
Size: 1947 376 742 85 1071 2174
Fragments arranged by size:
2174 1947 1071 742 636 399 390 376
241 186 90 85 78 37
BspEI T'CCGG_A
Cuts at: 3670 8115 3670
Size: 4445 4007
BspHI T'CATG_A
Cuts at: 3768 4771 6341 8071 3768
Size: 1003 1570 1730 4149
Fragments arranged by size:
4149 1730 1570 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2772 3220 3863 5807 844
Size: 1314 614 448 643 1944 3489
Fragments arranged by size:
3489 1944 1314 643 614 448
BsrBI CCG'CTC
Cuts at: 1394 1823 3880 6339 1394
Size: 429 2057 2459 3507
Fragments arranged by size:
3507 2459 2057 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 3378 4203 5301 6947 7121 65
Size: 1183 2046 84 825 1098 1646 174 1396
Fragments arranged by size:
2046 1646 1396 1183 1098 825 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 7096 7283 1700
Size: 110 5286 187 2869
Fragments arranged by size:
5286 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3958 96
Size: 3862 4590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8452
BssSI C'ACGA_G
Cuts at: 6512 7524 6512
Size: 1012 7440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8452
BstBI TT'CG_AA
Cuts at: 3982 7256 3982
Size: 3274 5178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8452
BstXI CCAn_nnnn'nTGG
Cuts at: 2702 3760 2702
Size: 1058 7394
BstYI r'GATC_y
Cuts at: 1972 3613 3802 4351 4916 5083 5782 6149 6534
Size: 1641 189 549 565 167 699 367 385
Cuts at: 6534 6551 8003 8089 8101 8447 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1641 1452 699 565 549 385 367
346 189 167 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2323 2370 4005 2323
Size: 47 1635 6770
Fragments arranged by size:
6770 1635 47
BtgI C'CryG_G
Cuts at: 513 1065 2004 3595 4261 513
Size: 552 939 1591 666 4704
Fragments arranged by size:
4704 1591 939 666 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2107 2241 4629 487
Size: 683 56 881 134 2388 4310
Fragments arranged by size:
4310 2388 881 683 134 56
BtsI GCAGTG_nn'
Cuts at: 3456 3468 3897 4391 5968 6762 6782 3456
Size: 12 429 494 1577 794 20 5126
Fragments arranged by size:
5126 1577 794 494 429 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3352 3387 529
Size: 35 2788 35 5594
Fragments arranged by size:
5594 2788 35 35
DraI TTT'AAA
Cuts at: 3967 5055 5756 3967
Size: 1088 701 6663
Fragments arranged by size:
6663 1088 701
DraIII CAC_nnn'GTG
Cuts at: 2434 2434
Size: 8452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2141 7459 809
Size: 507 825 5318 1802
Fragments arranged by size:
5318 1802 825 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3008 5899
Size: 54 1425 50 275 18 177 1001 2891
Cuts at: 5899 6788 8417 8
Size: 889 1629 43
Fragments arranged by size:
2891 1629 1425 1001 889 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5899 1537
Size: 275 4087 4090
Fragments arranged by size:
4090 4087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2281 2497 3436 3887 4143 5055 6382 1353
Size: 928 216 939 451 256 912 1327 3423
Fragments arranged by size:
3423 1327 939 928 912 451 256 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7039 7413 7559 387
Size: 1064 138 5450 374 146 1280
Fragments arranged by size:
5450 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2792 3446 2792
Size: 654 7798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2641 3621 3689 3868 4044 4182 4667
Size: 393 1009 980 68 179 176 138 485
Cuts at: 4667 5091 6515 7112 7899 8167 1239
Size: 424 1424 597 787 268 1524
Fragments arranged by size:
1524 1424 1009 980 787 597 485 424
393 268 179 176 138 68
EcoNI CCTnn'n_nnAGG
Cuts at: 3870 4176 3870
Size: 306 8146
EcoO109I rG'GnC_Cy
Cuts at: 2419 3242 3258 3259 4895 2419
Size: 823 16 1 1636 5976
Fragments arranged by size:
5976 1636 823 16 1
EcoRI G'AATT_C
Cuts at: 4118 5761 6139 4118
Size: 1643 378 6431
Fragments arranged by size:
6431 1643 378
EcoRV GAT'ATC
Cuts at: 2203 2203
Size: 8452
FspI TGC'GCA
Cuts at: 6958 6958
Size: 8452
HaeII r_GCGC'y
Cuts at: 1678 7599 1678
Size: 5921 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3845 3877 3879 3911 4316
Size: 32 135 32 2273 32 2 32 405
Cuts at: 4316 4348 7093 7125 7167 7199 1373
Size: 32 2745 32 42 32 2626
Fragments arranged by size:
2745 2626 2273 405 135 42 32 32
32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2980 4237 4457 5796 6042
Size: 988 281 208 834 1257 220 1339 246
Cuts at: 6042 669
Size: 3079
Fragments arranged by size:
3079 1339 1257 988 834 281 246 220
208
HindIII A'AGCT_T
Cuts at: 748 4164 748
Size: 3416 5036
HpaI GTT'AAC
Cuts at: 6042 6042
Size: 8452
KpnI G_GTAC'C
Cuts at: 5777 5777
Size: 8452
MfeI C'AATT_G
Cuts at: 4191 5189 6051 4191
Size: 998 862 6592
Fragments arranged by size:
6592 998 862
MluI A'CGCG_T
Cuts at: 8328 8328
Size: 8452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3565 7566 7750 1305
Size: 2260 4001 184 2007
Fragments arranged by size:
4001 2260 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3010 10
Size: 54 1425 343 177 1001 5452
Fragments arranged by size:
5452 1425 1001 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2967 3461 3797 6410 6769 6928 518
Size: 648 1801 494 336 2613 359 159 2042
Fragments arranged by size:
2613 2042 1801 648 494 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2720 2784 3334 5868 6545 7693 7938
Size: 409 705 64 550 2534 677 1148 245
Cuts at: 7938 1606
Size: 2120
Fragments arranged by size:
2534 2120 1148 705 677 550 409 245
64
NcoI C'CATG_G
Cuts at: 513 1065 2004 4261 513
Size: 552 939 2257 4704
Fragments arranged by size:
4704 2257 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8452
NotI GC'GGCC_GC
Cuts at: 5899 5899
Size: 8452
NsiI A_TGCA'T
Cuts at: 3370 4303 5572 3370
Size: 933 1269 6250
Fragments arranged by size:
6250 1269 933
NspI r_CATG'y
Cuts at: 1132 1229 2167 2807 3372 4079 5570 5810 7355
Size: 97 938 640 565 707 1491 240 1545
Cuts at: 7355 1132
Size: 2229
Fragments arranged by size:
2229 1545 1491 938 707 640 565 240
97
PasI CC'CwG_GG
Cuts at: 1348 3807 1348
Size: 2459 5993
PciI A'CATG_T
Cuts at: 2803 7351 2803
Size: 4548 3904
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2261 3017 1108
Size: 1153 756 6543
Fragments arranged by size:
6543 1153 756
PfoI T'CCnGG_A
Cuts at: 2130 2130
Size: 8452
PmeI GTTT'AAAC
Cuts at: 5756 5756
Size: 8452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3603 3635 6521 6553 8034 8066 3603
Size: 32 2886 32 1481 32 3989
Fragments arranged by size:
3989 2886 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 3242 4895 2419
Size: 823 1653 5976
Fragments arranged by size:
5976 1653 823
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8452
PsiI TTA'TAA
Cuts at: 2957 5130 6022 2957
Size: 2173 892 5387
Fragments arranged by size:
5387 2173 892
PspOMI G'GGCC_C
Cuts at: 3258 3258
Size: 8452
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4953 4985 4953
Size: 32 8420
PstI C_TGCA'G
Cuts at: 830 3463 3904 5804 830
Size: 2633 441 1900 3478
Fragments arranged by size:
3478 2633 1900 441
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8452
PvuII CAG'CTG
Cuts at: 2720 3334 2720
Size: 614 7838
SacI G_AGCT'C
Cuts at: 2794 3448 2794
Size: 654 7798
SalI G'TCGA_C
Cuts at: 1936 2144 5794 1936
Size: 208 3650 4594
Fragments arranged by size:
4594 3650 208
SanDI GG'GwC_CC
Cuts at: 2419 2419
Size: 8452
SapI GCTCTTCn'nnn_
Cuts at: 2497 3436 3887 2497
Size: 939 451 7062
Fragments arranged by size:
7062 939 451
SbfI CC_TGCA'GG
Cuts at: 5804 5804
Size: 8452
ScaI AGT'ACT
Cuts at: 6700 8412 6700
Size: 1712 6740
SexAI A'CCwGG_T
Cuts at: 3970 3970
Size: 8452
SfcI C'TryA_G
Cuts at: 826 1045 2241 2452 2983 3108 3459 3900 4673
Size: 219 1196 211 531 125 351 441 773
Cuts at: 4673 5800 6935 7616 7807 826
Size: 1127 1135 681 191 1471
Fragments arranged by size:
1471 1196 1135 1127 773 681 531 441
351 219 211 191 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8452
SmaI CCC'GGG
Cuts at: 1872 2151 5779 1872
Size: 279 3628 4545
Fragments arranged by size:
4545 3628 279
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 2378 2496 2656 3492 3814
Size: 197 926 351 84 118 160 836 322
Cuts at: 3814 4756 5424 6556 7457 7719 820
Size: 942 668 1132 901 262 1553
Fragments arranged by size:
1553 1132 942 926 901 836 668 351
322 262 197 160 118 84
SnaBI TAC'GTA
Cuts at: 493 6243 493
Size: 5750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8452
SphI G_CATG'C
Cuts at: 1132 2167 5810 1132
Size: 1035 3643 3774
Fragments arranged by size:
3774 3643 1035
SspI AAT'ATT
Cuts at: 5 52 6376 5
Size: 47 6324 2081
Fragments arranged by size:
6324 2081 47
StuI AGG'CCT
Cuts at: 1517 2931 5314 5598 1517
Size: 1414 2383 284 4371
Fragments arranged by size:
4371 2383 1414 284
StyI C'CwwG_G
Cuts at: 513 1065 2004 3262 4177 4261 5891 513
Size: 552 939 1258 915 84 1630 3074
Fragments arranged by size:
3074 1630 1258 939 915 552 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4846 5432 6479 6496 6649 6834 7279
Size: 1255 3266 586 1047 17 153 185 445
Cuts at: 7279 325
Size: 1498
Fragments arranged by size:
3266 1498 1255 1047 586 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3958 4274 6698
Size: 274 80 33 51 1443 1981 316 2424
Cuts at: 6698 8410 96
Size: 1712 138
Fragments arranged by size:
2424 1981 1712 1443 316 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3013 3045 5374 5406 5677 5709 8412 8444 3013
Size: 32 2329 32 271 32 2703 32 3021
Fragments arranged by size:
3021 2703 2329 271 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8452
XbaI T'CTAG_A
Cuts at: 3236 3448 5788 3236
Size: 212 2340 5900
Fragments arranged by size:
5900 2340 212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8452
XmaI C'CCGG_G
Cuts at: 1870 2149 5777 1870
Size: 279 3628 4545
Fragments arranged by size:
4545 3628 279
XmnI GAAnn'nnTTC
Cuts at: 4167 6581 4167
Size: 2414 6038
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6260 276
Size: 53 83 186 936 4726 2468
Fragments arranged by size:
4726 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII
SacII SfiI SfoI SrfI SwaI XcmI