Restriction map for
pFN21AB3003
(Circular) (Six-base) MAPSORT of: pFN21AB3003.seq Check: 2861 from: 1 to: 6604
pFN21AB3003 6604 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4414 278
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3947 1937
Size: 2010 4594
Acc65I G'GTAC_C
Cuts at: 3204 3925 3204
Size: 721 5883
AclI AA'CG_TT
Cuts at: 1563 4731 5104 1563
Size: 3168 373 3063
Fragments arranged by size:
3168 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2563 2948 3387 4667 6051 6319 1632
Size: 931 385 439 1280 1384 268 1917
Fragments arranged by size:
1917 1384 1280 931 439 385 268
AfeI AGC'GCT
Cuts at: 2375 2375
Size: 6604
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6407
AflIII A'CryG_T
Cuts at: 1203 5503 6480 1203
Size: 4300 977 1327
Fragments arranged by size:
4300 1327 977
AgeI A'CCGG_T
Cuts at: 5435 5435
Size: 6604
AhdI GACnn_n'nnGTC
Cuts at: 5333 5333
Size: 6604
AleI CACnn'nnGTG
Cuts at: 1166 3258 1166
Size: 2092 4512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6604
AlwNI CAG_nnn'CTG
Cuts at: 2867 3614 4347 5919 2867
Size: 747 733 1572 3552
Fragments arranged by size:
3552 1572 747 733
ApaLI G'TGCA_C
Cuts at: 4661 5817 4661
Size: 1156 5448
ApoI r'AATT_y
Cuts at: 1493 3913 4139 4291 6400 1493
Size: 2420 226 152 2109 1697
Fragments arranged by size:
2420 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5158 160
Size: 872 4126 1606
Fragments arranged by size:
4126 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
AvaI C'yCGr_G
Cuts at: 1870 1943 2871 2901 2931 3066 3722 3929 1870
Size: 73 928 30 30 135 656 207 4545
Fragments arranged by size:
4545 928 656 207 135 73 30 30
AvrII C'CTAG_G
Cuts at: 3124 3124
Size: 6604
BamHI G'GATC_C
Cuts at: 3775 3934 4301 3775
Size: 159 367 6078
Fragments arranged by size:
6078 367 159
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2912 3204 3892 3925 5380
Size: 325 217 627 1125 292 688 33 1455
Cuts at: 5380 618
Size: 1842
Fragments arranged by size:
1842 1455 1125 688 627 325 292 217
33
BanII G_rGCy'C
Cuts at: 1391 2572 2922 3621 3723 3923 1391
Size: 1181 350 699 102 200 4072
Fragments arranged by size:
4072 1181 699 350 200 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2132 2777 3546 928
Size: 971 233 645 769 3986
Fragments arranged by size:
3986 971 769 645 233
BbvCI CC'TCA_GC
Cuts at: 2674 3479 2674
Size: 805 5799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4795 4829 1903
Size: 34 50 34 2774 34 3678
Fragments arranged by size:
3678 2774 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4496 5706 4496
Size: 1210 5394
BclI T'GATC_A
Cuts at: 1548 3756 1548
Size: 2208 4396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 3135 5215
Size: 107 122 71 631 605 635 828 2080
Cuts at: 5215 136
Size: 1525
Fragments arranged by size:
2080 1525 828 635 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6599 6599
Size: 6604
BlpI GC'TnA_GC
Cuts at: 2197 4021 2197
Size: 1824 4780
Bme1580I G_kGCm'C
Cuts at: 1790 4665 5821 1790
Size: 2875 1156 2573
Fragments arranged by size:
2875 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2597 2855 4335
Size: 416 53 83 399 24 1180 258 1480
Cuts at: 4335 5293 442
Size: 958 1753
Fragments arranged by size:
1753 1480 1180 958 416 399 258 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2253 2636 2667 2674 3380 5264 1239
Size: 1014 383 31 7 706 1884 2579
Fragments arranged by size:
2579 1884 1014 706 383 31 7
Bpu10I CC'TnA_GC
Cuts at: 2674 3479 3615 2674
Size: 805 136 5663
Fragments arranged by size:
5663 805 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2455 4729 5594 5892 2063
Size: 392 2274 865 298 2775
Fragments arranged by size:
2775 2274 865 392 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2812 3365 5267 882
Size: 617 1313 553 1902 2219
Fragments arranged by size:
2219 1902 1313 617 553
BsaAI yAC'GTr
Cuts at: 493 1204 4395 493
Size: 711 3191 2702
Fragments arranged by size:
3191 2702 711
BsaBI GATnn'nnATC
Cuts at: 6598 6598
Size: 6604
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4411 4793 5428 275
Size: 53 83 186 936 2878 382 635 1451
Fragments arranged by size:
2878 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5037 5435 5709 5856 6267 5037
Size: 398 274 147 411 5374
Fragments arranged by size:
5374 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3378 3408 3794 3824 1178
Size: 30 165 30 1975 30 386 30 3958
Fragments arranged by size:
3958 1975 386 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2549 2662 2919 2960 3251 3496 3542
Size: 988 375 113 257 41 291 245 46
Cuts at: 3542 3803 1186
Size: 261 3987
Fragments arranged by size:
3987 988 375 291 261 257 245 113
46 41
BseYI C'CCAG_C
Cuts at: 2240 2376 2620 2777 2793 2848 2950 3338 3779
Size: 136 244 157 16 55 102 388 441
Cuts at: 3779 5807 6303 2240
Size: 2028 496 2541
Fragments arranged by size:
2541 2028 496 441 388 244 157 136
102 55 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2085 1163
Size: 573 349 5682
Fragments arranged by size:
5682 573 349
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4054 4815 5843 1540
Size: 275 186 2053 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2878 3396 3621 3723 4665 4750 5821 2878
Size: 518 225 102 942 85 1071 3661
Fragments arranged by size:
3661 1071 942 518 225 102 85
BsmI GAATG_Cn'
Cuts at: 3818 4115 4208 3818
Size: 297 93 6214
Fragments arranged by size:
6214 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2572 2878 2922 3396 3621 3723 3923
Size: 399 782 306 44 474 225 102 200
Cuts at: 3923 4665 4750 5821 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 474 399 306 225
200 102 85 44
BspEI T'CCGG_A
Cuts at: 6267 6267
Size: 6604
BspHI T'CATG_A
Cuts at: 4493 6223 4493
Size: 1730 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3959 844
Size: 3115 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3009 3679 4491 1394
Size: 429 1186 670 812 3507
Fragments arranged by size:
3507 1186 812 670 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3856 5099 5273 65
Size: 1183 2608 1243 174 1396
Fragments arranged by size:
2608 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5248 5435 1700
Size: 110 3438 187 2869
Fragments arranged by size:
3438 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6604
BssSI C'ACGA_G
Cuts at: 4664 5676 4664
Size: 1012 5592
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6604
BstBI TT'CG_AA
Cuts at: 5408 5408
Size: 6604
BstEII G'GTnAC_C
Cuts at: 1184 2269 1184
Size: 1085 5519
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 3884 2137
Size: 1747 4857
BstYI r'GATC_y
Cuts at: 1972 2013 2958 3775 3837 3934 4301 4686 4703
Size: 41 945 817 62 97 367 385 17
Cuts at: 4703 6155 6241 6253 6599 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 945 817 385 367 346 97
86 62 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3558 513
Size: 552 2493 3559
Fragments arranged by size:
3559 2493 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5865
Fragments arranged by size:
5865 683 56
BtsI GCAGTG_nn'
Cuts at: 2311 2892 3238 4120 4914 4934 2311
Size: 581 346 882 794 20 3981
Fragments arranged by size:
3981 882 794 581 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6569
DraI TTT'AAA
Cuts at: 3908 3908
Size: 6604
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 6604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 2351 5611 809
Size: 507 942 93 3260 1802
Fragments arranged by size:
3260 1802 942 507 93
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2515 3053 3520
Size: 54 1425 50 275 18 685 538 467
Cuts at: 3520 4051 4940 6569 8
Size: 531 889 1629 43
Fragments arranged by size:
1629 1425 889 685 538 531 467 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4051 1537
Size: 275 2239 4090
Fragments arranged by size:
4090 2239 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2579 2732 3363 4534 1353
Size: 1226 153 631 1171 3423
Fragments arranged by size:
3423 1226 1171 631 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2006 2012 3464 3697 5191 5565
Size: 1064 138 417 6 1452 233 1494 374
Cuts at: 5565 5711 387
Size: 146 1280
Fragments arranged by size:
1494 1452 1280 1064 417 374 233 146
138 6
EcoICRI GAG'CTC
Cuts at: 3619 3721 3619
Size: 102 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2563 2636 2667 2674 2948 3380
Size: 393 621 310 73 31 7 274 432
Cuts at: 3380 3387 4667 5264 6051 6319 1239
Size: 7 1280 597 787 268 1524
Fragments arranged by size:
1524 1280 787 621 597 432 393 310
274 268 73 31 7 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3199 3199
Size: 6604
EcoO109I rG'GnC_Cy
Cuts at: 2260 2482 2627 3028 3121 3793 2260
Size: 222 145 401 93 672 5071
Fragments arranged by size:
5071 672 401 222 145 93
EcoRI G'AATT_C
Cuts at: 3913 4291 3913
Size: 378 6226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 6604
FspI TGC'GCA
Cuts at: 5110 5110
Size: 6604
HaeII r_GCGC'y
Cuts at: 1678 2241 2377 2550 5751 1678
Size: 563 136 173 3201 2531
Fragments arranged by size:
3201 2531 563 173 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 3126 3158 3233
Size: 32 135 32 899 32 623 32 75
Cuts at: 3233 3265 3794 3826 5245 5277 5319 5351 1373
Size: 32 529 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1419 899 623 529 135 75 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3948 4194 669
Size: 988 281 2010 246 3079
Fragments arranged by size:
3079 2010 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2510 3284 748
Size: 1762 774 4068
Fragments arranged by size:
4068 1762 774
HpaI GTT'AAC
Cuts at: 4194 4194
Size: 6604
KpnI G_GTAC'C
Cuts at: 3208 3929 3208
Size: 721 5883
MfeI C'AATT_G
Cuts at: 4203 4203
Size: 6604
MluI A'CGCG_T
Cuts at: 6480 6480
Size: 6604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2727 2861 5718 5902 1305
Size: 825 597 134 2857 184 2007
Fragments arranged by size:
2857 2007 825 597 184 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2517 3522 10
Size: 54 1425 343 685 1005 3092
Fragments arranged by size:
3092 1425 1005 685 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3258 3769 4562 4921 5080 518
Size: 648 969 1123 511 793 359 159 2042
Fragments arranged by size:
2042 1123 969 793 648 511 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2642 2797 3258 3267 3332 4020 4697
Size: 625 411 155 461 9 65 688 677
Cuts at: 4697 5845 6090 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 688 677 625 461 411 245
155 65 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6052
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6604
NotI GC'GGCC_GC
Cuts at: 4051 4051
Size: 6604
NspI r_CATG'y
Cuts at: 1132 1229 2335 3181 3962 5507 1132
Size: 97 1106 846 781 1545 2229
Fragments arranged by size:
2229 1545 1106 846 781 97
PasI CC'CwG_GG
Cuts at: 1348 3668 1348
Size: 2320 4284
PciI A'CATG_T
Cuts at: 5503 5503
Size: 6604
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6604
PfoI T'CCnGG_A
Cuts at: 3396 3396
Size: 6604
PmeI GTTT'AAAC
Cuts at: 3908 3908
Size: 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6186 6218 4673
Size: 32 1481 32 5059
Fragments arranged by size:
5059 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2627 3121 2260
Size: 367 494 5743
Fragments arranged by size:
5743 494 367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6604
PsiI TTA'TAA
Cuts at: 4174 4174
Size: 6604
PspXI vC'TCGA_Gb
Cuts at: 3722 3722
Size: 6604
PstI C_TGCA'G
Cuts at: 830 2042 3956 830
Size: 1212 1914 3478
Fragments arranged by size:
3478 1914 1212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6604
PvuII CAG'CTG
Cuts at: 2231 2642 2797 3332 2231
Size: 411 155 535 5503
Fragments arranged by size:
5503 535 411 155
SacI G_AGCT'C
Cuts at: 3621 3723 3621
Size: 102 6502
SalI G'TCGA_C
Cuts at: 1936 3946 1936
Size: 2010 4594
SbfI CC_TGCA'GG
Cuts at: 3956 3956
Size: 6604
ScaI AGT'ACT
Cuts at: 4852 6564 4852
Size: 1712 4892
SfcI C'TryA_G
Cuts at: 826 1045 2038 2144 2410 3841 3952 5087 5768
Size: 219 993 106 266 1431 111 1135 681
Cuts at: 5768 5959 826
Size: 191 1471
Fragments arranged by size:
1471 1431 1135 993 681 266 219 191
111 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 3135 3135
Size: 6604
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6604
SmaI CCC'GGG
Cuts at: 1872 3068 3931 1872
Size: 1196 863 4545
Fragments arranged by size:
4545 1196 863
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2434 3722 4708 5609 5871
Size: 197 926 135 356 1288 986 901 262
Cuts at: 5871 820
Size: 1553
Fragments arranged by size:
1553 1288 986 926 901 356 262 197
135
SnaBI TAC'GTA
Cuts at: 493 4395 493
Size: 3902 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6604
SphI G_CATG'C
Cuts at: 1132 3181 3962 1132
Size: 2049 781 3774
Fragments arranged by size:
3774 2049 781
SspI AAT'ATT
Cuts at: 5 52 4528 5
Size: 47 4476 2081
Fragments arranged by size:
4476 2081 47
StuI AGG'CCT
Cuts at: 1517 3129 3248 1517
Size: 1612 119 4873
Fragments arranged by size:
4873 1612 119
StyI C'CwwG_G
Cuts at: 513 1065 2112 3124 4043 513
Size: 552 1047 1012 919 3074
Fragments arranged by size:
3074 1047 1012 919 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3148 4631 4648 4801 4986 5431 325
Size: 1255 1568 1483 17 153 185 445 1498
Fragments arranged by size:
1568 1498 1483 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3111 4850 6562
Size: 274 80 33 51 1443 1134 1739 1712
Cuts at: 6562 96
Size: 138
Fragments arranged by size:
1739 1712 1443 1134 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3378 3410 6564 6596 3378
Size: 32 3154 32 3386
Fragments arranged by size:
3386 3154 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2384 1316
Size: 1068 5536
XbaI T'CTAG_A
Cuts at: 3940 3940
Size: 6604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3530 3530
Size: 6604
XhoI C'TCGA_G
Cuts at: 1943 3722 1943
Size: 1779 4825
XmaI C'CCGG_G
Cuts at: 1870 3066 3929 1870
Size: 1196 863 4545
Fragments arranged by size:
4545 1196 863
XmnI GAAnn'nnTTC
Cuts at: 2073 3002 4733 2073
Size: 929 1731 3944
Fragments arranged by size:
3944 1731 929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4412 276
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI
SwaI