Restriction map for
pFN21AB2389
(Circular) (Six-base) MAPSORT of: pFN21AB2389.seq Check: 2466 from: 1 to: 7879
pFN21AB2389 7879 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2979 3115 3427 5042 2979
Size: 136 312 1615 5816
Fragments arranged by size:
5816 1615 312 136
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2493 3396 5689 278
Size: 53 83 186 936 957 903 2293 2468
Fragments arranged by size:
2468 2293 957 936 903 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4005 5222 1937
Size: 2068 1217 4594
Fragments arranged by size:
4594 2068 1217
Acc65I G'GTAC_C
Cuts at: 5200 5200
Size: 7879
AclI AA'CG_TT
Cuts at: 1563 6006 6379 1563
Size: 4443 373 3063
Fragments arranged by size:
4443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3052 3206 3980 4084 4111 4346 4730 5157
Size: 1420 154 774 104 27 235 384 427
Cuts at: 5157 5942 7326 7594 1632
Size: 785 1384 268 1917
Fragments arranged by size:
1917 1420 1384 785 774 427 384 268
235 154 104 27
AfeI AGC'GCT
Cuts at: 2432 5102 2432
Size: 2670 5209
AflII C'TTAA_G
Cuts at: 820 1017 2152 820
Size: 197 1135 6547
Fragments arranged by size:
6547 1135 197
AflIII A'CryG_T
Cuts at: 1203 2125 2443 2628 4047 6778 7755 1203
Size: 922 318 185 1419 2731 977 1327
Fragments arranged by size:
2731 1419 1327 977 922 318 185
AgeI A'CCGG_T
Cuts at: 6710 6710
Size: 7879
AhdI GACnn_n'nnGTC
Cuts at: 6608 6608
Size: 7879
AleI CACnn'nnGTG
Cuts at: 1166 4946 1166
Size: 3780 4099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4066 1184
Size: 2882 4997
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 7847
AlwNI CAG_nnn'CTG
Cuts at: 2389 2869 3932 4915 5622 7194 2389
Size: 480 1063 983 707 1572 3074
Fragments arranged by size:
3074 1572 1063 983 707 480
ApaI G_GGCC'C
Cuts at: 2702 3343 2702
Size: 641 7238
ApaLI G'TGCA_C
Cuts at: 3668 5936 7092 3668
Size: 2268 1156 4455
Fragments arranged by size:
4455 2268 1156
ApoI r'AATT_y
Cuts at: 1493 3965 5188 5414 5566 7675 1493
Size: 2472 1223 226 152 2109 1697
Fragments arranged by size:
2472 2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6433 160
Size: 872 5401 1606
Fragments arranged by size:
5401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
AvaI C'yCGr_G
Cuts at: 1870 1943 2701 5204 1870
Size: 73 758 2503 4545
Fragments arranged by size:
4545 2503 758 73
AvrII C'CTAG_G
Cuts at: 2011 2011
Size: 7879
BamHI G'GATC_C
Cuts at: 2691 5209 5576 2691
Size: 2518 367 4994
Fragments arranged by size:
4994 2518 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3722 4161 5145 5200 6655
Size: 325 217 627 1935 439 984 55 1455
Cuts at: 6655 618
Size: 1842
Fragments arranged by size:
1935 1842 1455 984 627 439 325 217
55
BanII G_rGCy'C
Cuts at: 1391 2702 3343 3885 4209 4449 5198 1391
Size: 1311 641 542 324 240 749 4072
Fragments arranged by size:
4072 1311 749 641 542 324 240
BbeI G_GCGC'C
Cuts at: 5149 5149
Size: 7879
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 2475 3582 4404 928
Size: 971 393 183 1107 822 4403
Fragments arranged by size:
4403 1107 971 822 393 183
BbvCI CC'TCA_GC
Cuts at: 5121 5121
Size: 7879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3163 3197 6070 6104 1903
Size: 34 1226 34 2873 34 3678
Fragments arranged by size:
3678 2873 1226 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 5771 6981 3518
Size: 2253 1210 4416
Fragments arranged by size:
4416 2253 1210
BclI T'GATC_A
Cuts at: 1548 2214 1548
Size: 666 7213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 3650 6490
Size: 107 122 71 631 605 1060 918 2840
Cuts at: 6490 136
Size: 1525
Fragments arranged by size:
2840 1525 1060 918 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2160 4380 7874 2160
Size: 2220 3494 2165
Fragments arranged by size:
3494 2220 2165
BlpI GC'TnA_GC
Cuts at: 5296 5296
Size: 7879
Bme1580I G_kGCm'C
Cuts at: 1790 2636 2702 3343 3672 4347 5092 5940 7096
Size: 846 66 641 329 675 745 848 1156
Cuts at: 7096 1790
Size: 2573
Fragments arranged by size:
2573 1156 848 846 745 675 641 329
66
BmgBI CAC'GTC
Cuts at: 1318 2473 4522 1318
Size: 1155 2049 4675
Fragments arranged by size:
4675 2049 1155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2539 2925
Size: 416 53 83 399 24 711 411 386
Cuts at: 2925 5610 6568 442
Size: 2685 958 1753
Fragments arranged by size:
2685 1753 958 711 416 411 399 386
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 4261 4426 2182
Size: 2079 165 5635
Fragments arranged by size:
5635 2079 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4055 4223 4462 4664 5066 6539 1239
Size: 2816 168 239 202 402 1473 2579
Fragments arranged by size:
2816 2579 1473 402 239 202 168
Bpu10I CC'TnA_GC
Cuts at: 2612 5121 2612
Size: 2509 5370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3962 4003 6004 6869 7167 3962
Size: 41 2001 865 298 4674
Fragments arranged by size:
4674 2001 865 298 41
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2930 3136 4232 6542 882
Size: 617 1431 206 1096 2310 2219
Fragments arranged by size:
2310 2219 1431 1096 617 206
BsaAI yAC'GTr
Cuts at: 493 1204 4819 5670 493
Size: 711 3615 851 2702
Fragments arranged by size:
3615 2702 851 711
BsaBI GATnn'nnATC
Cuts at: 2219 4025 4196 7873 2219
Size: 1806 171 3677 2225
Fragments arranged by size:
3677 2225 1806 171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2490 3393 5146 5686
Size: 53 83 186 936 957 903 1753 540
Cuts at: 5686 6068 6703 275
Size: 382 635 1451
Fragments arranged by size:
1753 1451 957 936 903 635 540 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6312 6710 6984 7131 7542 6312
Size: 398 274 147 411 6649
Fragments arranged by size:
6649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4145 4175 4196 4226 1178
Size: 30 165 30 2742 30 21 30 4831
Fragments arranged by size:
4831 2742 165 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2155 3005 4085 4550 4646 4820 1186
Size: 969 850 1080 465 96 174 4245
Fragments arranged by size:
4245 1080 969 850 465 174 96
BseYI C'CCAG_C
Cuts at: 2184 7082 7578 2184
Size: 4898 496 2485
Fragments arranged by size:
4898 2485 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2948 3718 3803 4615 4949 1163
Size: 573 1212 770 85 812 334 4093
Fragments arranged by size:
4093 1212 812 770 573 334 85
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5329 6090 7118 1540
Size: 275 186 3328 761 1028 2301
Fragments arranged by size:
3328 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3009 3179 3672 3885 4209 5940 6025 7096 3009
Size: 170 493 213 324 1731 85 1071 3792
Fragments arranged by size:
3792 1731 1071 493 324 213 170 85
BsmI GAATG_Cn'
Cuts at: 5390 5483 5390
Size: 93 7786
BsmBI CGTCTCn'nnnn_
Cuts at: 4480 4480
Size: 7879
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2636 2702 3009 3179 3343 3672 3885
Size: 399 846 66 307 170 164 329 213
Cuts at: 3885 4209 4347 4449 5092 5198 5940 6025 7096
Size: 324 138 102 643 106 742 85 1071
Cuts at: 7096 1391
Size: 2174
Fragments arranged by size:
2174 1071 846 742 643 399 329 324
307 213 170 164 138 106 102 85
66
BspEI T'CCGG_A
Cuts at: 7542 7542
Size: 7879
BspHI T'CATG_A
Cuts at: 5768 7498 5768
Size: 1730 6149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2580 2979 3115 3427 3494 4085 5042 5234
Size: 1736 399 136 312 67 591 957 192
Cuts at: 5234 844
Size: 3489
Fragments arranged by size:
3489 1736 957 591 399 312 192 136
67
BsrBI CCG'CTC
Cuts at: 1394 1823 5766 1394
Size: 429 3943 3507
Fragments arranged by size:
3943 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2562 3219 5115 6374 6548 65
Size: 1183 1314 657 1896 1259 174 1396
Fragments arranged by size:
1896 1396 1314 1259 1183 657 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3042 6523 6710 1700
Size: 110 1232 3481 187 2869
Fragments arranged by size:
3481 2869 1232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7879
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7879
BssSI C'ACGA_G
Cuts at: 5093 5939 6951 5093
Size: 846 1012 6021
Fragments arranged by size:
6021 1012 846
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2971 3004 4585 794
Size: 2177 33 1581 4088
Fragments arranged by size:
4088 2177 1581 33
BstBI TT'CG_AA
Cuts at: 6683 6683
Size: 7879
BstEII G'GTnAC_C
Cuts at: 1184 2519 1184
Size: 1335 6544
BstXI CCAn_nnnn'nTGG
Cuts at: 2947 3226 3811 2947
Size: 279 585 7015
Fragments arranged by size:
7015 585 279
BstYI r'GATC_y
Cuts at: 1972 2160 2679 2691 3429 4020 4183 4380 5209
Size: 188 519 12 738 591 163 197 829
Cuts at: 5209 5576 5961 5978 7430 7516 7528 7874 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 829 738 591 519 385 367
346 197 188 163 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2088 3164 3767 4319 4533 4602 4751 2088
Size: 1076 603 552 214 69 149 5216
Fragments arranged by size:
5216 1076 603 552 214 149 69
BtgI C'CryG_G
Cuts at: 513 1065 2004 2665 2814 3225 3648 513
Size: 552 939 661 149 411 423 4744
Fragments arranged by size:
4744 939 661 552 423 411 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7140
Fragments arranged by size:
7140 683 56
BtsI GCAGTG_nn'
Cuts at: 3750 5395 6189 6209 3750
Size: 1645 794 20 5420
Fragments arranged by size:
5420 1645 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3621 3656 4357 4392 529
Size: 35 3057 35 701 35 4016
Fragments arranged by size:
4016 3057 701 35 35 35
DraI TTT'AAA
Cuts at: 5183 5183
Size: 7879
DraIII CAC_nnn'GTG
Cuts at: 2251 2251
Size: 7879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6886 809
Size: 507 5570 1802
Fragments arranged by size:
5570 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2652 2724
Size: 54 1425 50 275 18 237 585 72
Cuts at: 2724 2811 2919 3228 4974 5326 6215 7844 8
Size: 87 108 309 1746 352 889 1629 43
Fragments arranged by size:
1746 1629 1425 889 585 352 309 275
237 108 87 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5326 1537
Size: 275 3514 4090
Fragments arranged by size:
4090 3514 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 3350 3942 3993 4103 4322 4751 5809
Size: 992 1005 592 51 110 219 429 1058
Cuts at: 5809 1353
Size: 3423
Fragments arranged by size:
3423 1058 1005 992 592 429 219 110
51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3158 6466 6840 6986 387
Size: 1064 138 1569 3308 374 146 1280
Fragments arranged by size:
3308 1569 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3883 4207 3883
Size: 324 7555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3052 3206 3980 4055 4084 4111 4223
Size: 393 1420 154 774 75 29 27 112
Cuts at: 4223 4346 4462 4664 4730 5066 5157 5942 6539
Size: 123 116 202 66 336 91 785 597
Cuts at: 6539 7326 7594 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1420 787 785 774 597 393 336
268 202 154 123 116 112 91 75
66 29 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3110 4803 4919 3110
Size: 1693 116 6070
Fragments arranged by size:
6070 1693 116
EcoO109I rG'GnC_Cy
Cuts at: 2015 2530 3195 3339 3340 3532 3788 4912 2015
Size: 515 665 144 1 192 256 1124 4982
Fragments arranged by size:
4982 1124 665 515 256 192 144 1
EcoRI G'AATT_C
Cuts at: 5188 5566 5188
Size: 378 7501
EcoRV GAT'ATC
Cuts at: 3208 3208
Size: 7879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3316 4351 3316
Size: 1035 6844
FspI TGC'GCA
Cuts at: 6385 6385
Size: 7879
HaeII r_GCGC'y
Cuts at: 1678 2434 2870 4618 5104 5149 7026 1678
Size: 756 436 1748 486 45 1877 2531
Fragments arranged by size:
2531 1877 1748 756 486 436 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 2364 2396 2671
Size: 32 135 32 552 32 208 32 275
Cuts at: 2671 2703 4208 4240 4489 4521 6520 6552 6594
Size: 32 1505 32 249 32 1999 32 42
Cuts at: 6594 6626 1373
Size: 32 2626
Fragments arranged by size:
2626 1999 1505 552 275 249 208 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 5223 5469 669
Size: 988 281 517 2768 246 3079
Fragments arranged by size:
3079 2768 988 517 281 246
HindIII A'AGCT_T
Cuts at: 748 2364 4388 748
Size: 1616 2024 4239
Fragments arranged by size:
4239 2024 1616
HpaI GTT'AAC
Cuts at: 5469 5469
Size: 7879
KasI G'GCGC_C
Cuts at: 5145 5145
Size: 7879
KpnI G_GTAC'C
Cuts at: 5204 5204
Size: 7879
MfeI C'AATT_G
Cuts at: 5478 5478
Size: 7879
MluI A'CGCG_T
Cuts at: 2628 4047 7755 2628
Size: 1419 3708 2752
Fragments arranged by size:
3708 2752 1419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 2286 2669 6993 7177 1305
Size: 805 176 383 4324 184 2007
Fragments arranged by size:
4324 2007 805 383 184 176
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 2726 2813 2921 4976
Size: 54 1425 343 822 72 87 108 2055
Cuts at: 4976 10
Size: 2913
Fragments arranged by size:
2913 2055 1425 822 343 108 87 72
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 3224 3809 4946 4976 5837 6196
Size: 648 1779 279 585 1137 30 861 359
Cuts at: 6196 6355 518
Size: 159 2042
Fragments arranged by size:
2042 1779 1137 861 648 585 359 279
159 30
MspA1I CmG'CkG
Cuts at: 1606 2077 2667 3404 4482 4627 4883 5295 5972
Size: 471 590 737 1078 145 256 412 677
Cuts at: 5972 7120 7365 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1078 737 677 590 471 412
256 245 145
NarI GG'CG_CC
Cuts at: 5146 5146
Size: 7879
NcoI C'CATG_G
Cuts at: 513 1065 2004 3225 513
Size: 552 939 1221 5167
Fragments arranged by size:
5167 1221 939 552
NdeI CA'TA_TG
Cuts at: 387 3258 387
Size: 2871 5008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7879
NotI GC'GGCC_GC
Cuts at: 5326 5326
Size: 7879
NspI r_CATG'y
Cuts at: 1132 1229 2447 2743 5237 6782 1132
Size: 97 1218 296 2494 1545 2229
Fragments arranged by size:
2494 2229 1545 1218 296 97
PasI CC'CwG_GG
Cuts at: 1348 4424 1348
Size: 3076 4803
PciI A'CATG_T
Cuts at: 2443 6778 2443
Size: 4335 3544
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4117 4201 1108
Size: 3009 84 4786
Fragments arranged by size:
4786 3009 84
PfoI T'CCnGG_A
Cuts at: 2484 3453 4200 4854 2484
Size: 969 747 654 5509
Fragments arranged by size:
5509 969 747 654
PmeI GTTT'AAAC
Cuts at: 5183 5183
Size: 7879
PmlI CAC'GTG
Cuts at: 4819 4819
Size: 7879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 5948 5980 7461 7493 2063
Size: 32 3853 32 1481 32 2449
Fragments arranged by size:
3853 2449 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2015 2530 3788 4912 2015
Size: 515 1258 1124 4982
Fragments arranged by size:
4982 1258 1124 515
PshAI GACnn'nnGTC
Cuts at: 1613 4011 1613
Size: 2398 5481
PsiI TTA'TAA
Cuts at: 5449 5449
Size: 7879
PspOMI G'GGCC_C
Cuts at: 2698 3339 2698
Size: 641 7238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3499 3531 3499
Size: 32 7847
PstI C_TGCA'G
Cuts at: 830 2176 2241 4775 5231 830
Size: 1346 65 2534 456 3478
Fragments arranged by size:
3478 2534 1346 456 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7879
PvuII CAG'CTG
Cuts at: 2077 3404 4627 4883 2077
Size: 1327 1223 256 5073
Fragments arranged by size:
5073 1327 1223 256
SacI G_AGCT'C
Cuts at: 3885 4209 3885
Size: 324 7555
SacII CC_GC'GG
Cuts at: 2668 2668
Size: 7879
SalI G'TCGA_C
Cuts at: 1936 5221 1936
Size: 3285 4594
SanDI GG'GwC_CC
Cuts at: 3788 3788
Size: 7879
SapI GCTCTTCn'nnn_
Cuts at: 3942 4322 3942
Size: 380 7499
SbfI CC_TGCA'GG
Cuts at: 5231 5231
Size: 7879
ScaI AGT'ACT
Cuts at: 6127 7839 6127
Size: 1712 6167
SfcI C'TryA_G
Cuts at: 826 1045 2172 2237 4771 5227 6362 7043 7234
Size: 219 1127 65 2534 456 1135 681 191
Cuts at: 7234 826
Size: 1471
Fragments arranged by size:
2534 1471 1135 1127 681 456 219 191
65
SfiI GGCCn_nnn'nGGCC
Cuts at: 3650 3650
Size: 7879
SfoI GGC'GCC
Cuts at: 5147 5147
Size: 7879
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7879
SmaI CCC'GGG
Cuts at: 1872 2703 5206 1872
Size: 831 2503 4545
Fragments arranged by size:
4545 2503 831
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2152 3941 3982 5983 6884 7146
Size: 197 926 209 1789 41 2001 901 262
Cuts at: 7146 820
Size: 1553
Fragments arranged by size:
2001 1789 1553 926 901 262 209 197
41
SnaBI TAC'GTA
Cuts at: 493 5670 493
Size: 5177 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7879
SphI G_CATG'C
Cuts at: 1132 5237 1132
Size: 4105 3774
SspI AAT'ATT
Cuts at: 5 52 5803 5
Size: 47 5751 2081
Fragments arranged by size:
5751 2081 47
StuI AGG'CCT
Cuts at: 1517 2150 1517
Size: 633 7246
StyI C'CwwG_G
Cuts at: 513 1065 2004 2011 2229 3111 3198 3225 3858
Size: 552 939 7 218 882 87 27 633
Cuts at: 3858 3916 4149 4589 4889 5019 5318 513
Size: 58 233 440 300 130 299 3074
Fragments arranged by size:
3074 939 882 633 552 440 300 299
233 218 130 87 58 27 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2858 3740 3871 4437 4958 5906 5923
Size: 1255 1278 882 131 566 521 948 17
Cuts at: 5923 6076 6261 6706 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1278 1255 948 882 566 521 445
185 153 131 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6125 7837 96
Size: 274 80 33 51 1443 4148 1712 138
Fragments arranged by size:
4148 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2701 2733 2833 2865 4145 4177 4196
Size: 32 189 32 100 32 1280 32 19
Cuts at: 4196 4228 7839 7871 2480
Size: 32 3611 32 2488
Fragments arranged by size:
3611 2488 1280 189 100 32 32 32
32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 1316 2257 4862 1316
Size: 941 2605 4333
Fragments arranged by size:
4333 2605 941
XbaI T'CTAG_A
Cuts at: 4036 5215 4036
Size: 1179 6700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2647 4639 2647
Size: 1992 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7879
XmaI C'CCGG_G
Cuts at: 1870 2701 5204 1870
Size: 831 2503 4545
Fragments arranged by size:
4545 2503 831
XmnI GAAnn'nnTTC
Cuts at: 4331 4683 6008 4331
Size: 352 1325 6202
Fragments arranged by size:
6202 1325 352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2491 3394 5687 276
Size: 53 83 186 936 957 903 2293 2468
Fragments arranged by size:
2468 2293 957 936 903 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI RsrII SexAI SrfI
SwaI