Restriction map for
pFN21AB2260
(Circular) (Six-base) MAPSORT of: pFN21AB2260.seq Check: 7646 from: 1 to: 7333
pFN21AB2260 7333 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2257 4483 2257
Size: 2226 5107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5143 278
Size: 53 83 186 936 3607 2468
Fragments arranged by size:
3607 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3780 4676 1937
Size: 1843 896 4594
Fragments arranged by size:
4594 1843 896
Acc65I G'GTAC_C
Cuts at: 4654 4654
Size: 7333
AclI AA'CG_TT
Cuts at: 1563 5460 5833 1563
Size: 3897 373 3063
Fragments arranged by size:
3897 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 3064 3172 5396 6780 7048 1632
Size: 848 584 108 2224 1384 268 1917
Fragments arranged by size:
2224 1917 1384 848 584 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7136
AflIII A'CryG_T
Cuts at: 1203 4368 6232 7209 1203
Size: 3165 1864 977 1327
Fragments arranged by size:
3165 1864 1327 977
AgeI A'CCGG_T
Cuts at: 6164 6164
Size: 7333
AhdI GACnn_n'nnGTC
Cuts at: 6062 6062
Size: 7333
AleI CACnn'nnGTG
Cuts at: 1166 3771 1166
Size: 2605 4728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7333
AlwNI CAG_nnn'CTG
Cuts at: 2160 2743 5076 6648 2160
Size: 583 2333 1572 2845
Fragments arranged by size:
2845 2333 1572 583
ApaI G_GGCC'C
Cuts at: 2475 3069 3693 4072 4101 4420 2475
Size: 594 624 379 29 319 5388
Fragments arranged by size:
5388 624 594 379 319 29
ApaLI G'TGCA_C
Cuts at: 3764 5390 6546 3764
Size: 1626 1156 4551
Fragments arranged by size:
4551 1626 1156
ApoI r'AATT_y
Cuts at: 1493 4222 4642 4868 5020 7129 1493
Size: 2729 420 226 152 2109 1697
Fragments arranged by size:
2729 2109 1697 420 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5887 160
Size: 872 4855 1606
Fragments arranged by size:
4855 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7333
AvaI C'yCGr_G
Cuts at: 1870 1943 2373 2508 2997 4658 1870
Size: 73 430 135 489 1661 4545
Fragments arranged by size:
4545 1661 489 430 135 73
AvrII C'CTAG_G
Cuts at: 2968 2968
Size: 7333
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3437 3470 3437
Size: 33 7300
BamHI G'GATC_C
Cuts at: 4663 5030 4663
Size: 367 6966
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2223 2306 3137 3554 4654
Size: 325 217 627 436 83 831 417 1100
Cuts at: 4654 6109 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1100 831 627 436 417 325
217 83
BanII G_rGCy'C
Cuts at: 1391 2475 2550 2890 2955 2998 3069 3210 3248
Size: 1084 75 340 65 43 71 141 38
Cuts at: 3248 3465 3693 4072 4101 4420 4652 1391
Size: 217 228 379 29 319 232 4072
Fragments arranged by size:
4072 1084 379 340 319 232 228 217
141 75 71 65 43 38 29
BbeI G_GCGC'C
Cuts at: 3558 3558
Size: 7333
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6362
BbvCI CC'TCA_GC
Cuts at: 2834 3344 2834
Size: 510 6823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5524 5558 1903
Size: 34 3587 34 3678
Fragments arranged by size:
3678 3587 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5225 6435 5225
Size: 1210 6123
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2846 4067 4429
Size: 107 122 71 631 605 1174 1221 362
Cuts at: 4429 4527 5944 136
Size: 98 1417 1525
Fragments arranged by size:
1525 1417 1221 1174 631 605 362 122
107 98 71
BglII A'GATC_T
Cuts at: 3793 3858 7328 3793
Size: 65 3470 3798
Fragments arranged by size:
3798 3470 65
BlpI GC'TnA_GC
Cuts at: 4750 4750
Size: 7333
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2309 2475 3015 3069 3693 3768 4039
Size: 438 81 166 540 54 624 75 271
Cuts at: 4039 4072 4101 4420 5394 6550 1790
Size: 33 29 319 974 1156 2573
Fragments arranged by size:
2573 1156 974 624 540 438 319 271
166 81 75 54 33 29
BmgBI CAC'GTC
Cuts at: 1318 4369 1318
Size: 3051 4282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3140 5064 6022
Size: 416 53 83 399 24 1723 1924 958
Cuts at: 6022 442
Size: 1753
Fragments arranged by size:
1924 1753 1723 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7333
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 7333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 2779 3224 4067 4576 5993 1239
Size: 1345 195 445 843 509 1417 2579
Fragments arranged by size:
2579 1417 1345 843 509 445 195
Bpu10I CC'TnA_GC
Cuts at: 2834 3344 2834
Size: 510 6823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3774 3914 5458 6323 6621 3774
Size: 140 1544 865 298 4486
Fragments arranged by size:
4486 1544 865 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5996 882
Size: 617 4497 2219
Fragments arranged by size:
4497 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5124 493
Size: 711 3920 2702
Fragments arranged by size:
3920 2702 711
BsaBI GATnn'nnATC
Cuts at: 7327 7327
Size: 7333
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3555 3630 5140 5522
Size: 53 83 186 936 2022 75 1510 382
Cuts at: 5522 6157 275
Size: 635 1451
Fragments arranged by size:
2022 1510 1451 936 635 382 186 83
75 53
BsaWI w'CCGG_w
Cuts at: 2121 5766 6164 6438 6585 6996 2121
Size: 3645 398 274 147 411 2458
Fragments arranged by size:
3645 2458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 2777 2801 2807
Size: 30 165 30 840 30 504 24 6
Cuts at: 2807 2831 2837 1178
Size: 24 6 5674
Fragments arranged by size:
5674 840 504 165 30 30 30 24
24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2270 3332 3389 1186
Size: 1081 3 1062 57 5130
Fragments arranged by size:
5130 1081 1062 57 3
BseYI C'CCAG_C
Cuts at: 2409 2881 3955 4271 4419 4590 6536 7032 2409
Size: 472 1074 316 148 171 1946 496 2710
Fragments arranged by size:
2710 1946 1074 496 472 316 171 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 3311 3645 3989 4310 1163
Size: 573 534 1041 334 344 321 4186
Fragments arranged by size:
4186 1041 573 534 344 334 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3252 4511 4783 5544 6572
Size: 275 186 1024 227 1259 272 761 1028
Cuts at: 6572 1540
Size: 2301
Fragments arranged by size:
2301 1259 1028 1024 761 275 272 227
186
BsiHKAI G_wGCw'C
Cuts at: 2550 3768 5394 5479 6550 2550
Size: 1218 1626 85 1071 3333
Fragments arranged by size:
3333 1626 1218 1071 85
BsmI GAATG_Cn'
Cuts at: 3889 4844 4937 3889
Size: 955 93 6285
Fragments arranged by size:
6285 955 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2309 2475 2550 2890 2955 2998
Size: 399 438 81 166 75 340 65 43
Cuts at: 2998 3015 3069 3210 3248 3465 3693 3768 4039
Size: 17 54 141 38 217 228 75 271
Cuts at: 4039 4072 4101 4420 4652 5394 5479 6550 1391
Size: 33 29 319 232 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 340 319 271
232 228 217 166 141 85 81 75
75 65 54 43 38 33 29 17
BspEI T'CCGG_A
Cuts at: 6996 6996
Size: 7333
BspHI T'CATG_A
Cuts at: 2523 5222 6952 2523
Size: 2699 1730 2904
Fragments arranged by size:
2904 2699 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2257 2845 4438 4483 4688 844
Size: 1337 76 588 1593 45 205 3489
Fragments arranged by size:
3489 1593 1337 588 205 76 45
BsrBI CCG'CTC
Cuts at: 1394 1823 5220 1394
Size: 429 3397 3507
Fragments arranged by size:
3507 3397 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2440 3303 5828 6002 65
Size: 1183 1192 863 2525 174 1396
Fragments arranged by size:
2525 1396 1192 1183 863 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 2829 4488 5977 6164 1700
Size: 110 548 471 1659 1489 187 2869
Fragments arranged by size:
2869 1659 1489 548 471 187 110
BsrGI T'GTAC_A
Cuts at: 96 4180 96
Size: 4084 3249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7333
BssSI C'ACGA_G
Cuts at: 3471 5393 6405 3471
Size: 1922 1012 4399
Fragments arranged by size:
4399 1922 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4616 794
Size: 3822 3511
BstBI TT'CG_AA
Cuts at: 6137 6137
Size: 7333
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7333
BstXI CCAn_nnnn'nTGG
Cuts at: 3156 4427 3156
Size: 1271 6062
BstYI r'GATC_y
Cuts at: 1972 2272 3793 3858 4663 5030 5415 5432 6884
Size: 300 1521 65 805 367 385 17 1452
Cuts at: 6884 6970 6982 7328 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1521 1452 805 385 367 346 300
86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2445 2445
Size: 7333
BtgI C'CryG_G
Cuts at: 513 1065 2004 2478 2661 2934 3225 513
Size: 552 939 474 183 273 291 4621
Fragments arranged by size:
4621 939 552 474 291 273 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6594
Fragments arranged by size:
6594 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 2209 3050 3662 4308 4849 5643 5663 2172
Size: 37 841 612 646 541 794 20 3842
Fragments arranged by size:
3842 841 794 646 612 541 37 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7298
DraI TTT'AAA
Cuts at: 4637 4637
Size: 7333
DraIII CAC_nnn'GTG
Cuts at: 2293 2293
Size: 7333
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4448 6340 809
Size: 507 3132 1892 1802
Fragments arranged by size:
3132 1892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2753 3022 3161
Size: 54 1425 50 275 18 923 269 139
Cuts at: 3161 3228 3276 3866 4164 4194 4508 4780 5669
Size: 67 48 590 298 30 314 272 889
Cuts at: 5669 7298 8
Size: 1629 43
Fragments arranged by size:
1629 1425 923 889 590 314 298 275
272 269 139 67 54 50 48 43
30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 4508 4780 1537
Size: 275 1210 1486 272 4090
Fragments arranged by size:
4090 1486 1210 275 272
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 3083 3366 5263 1353
Size: 1373 357 283 1897 3423
Fragments arranged by size:
3423 1897 1373 357 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2682 3471 5920 6294 6440 387
Size: 1064 138 1093 789 2449 374 146 1280
Fragments arranged by size:
2449 1280 1093 1064 789 374 146 138
EcoICRI GAG'CTC
Cuts at: 2548 2548
Size: 7333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 2584 2779 3064 3172 3224 4067
Size: 393 848 104 195 285 108 52 843
Cuts at: 4067 4576 5396 5993 6780 7048 1239
Size: 509 820 597 787 268 1524
Fragments arranged by size:
1524 848 843 820 787 597 509 393
285 268 195 108 104 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 3347 3773 3905 4394 4610 2336
Size: 1011 426 132 489 216 5059
Fragments arranged by size:
5059 1011 489 426 216 132
EcoO109I rG'GnC_Cy
Cuts at: 2017 2217 2329 2440 2464 2472 2838 2986 3065
Size: 200 112 111 24 8 366 148 79
Cuts at: 3065 3066 3434 3690 3934 4068 4069 4097 4189
Size: 1 368 256 244 134 1 28 92
Cuts at: 4189 4416 4528 2017
Size: 227 112 4822
Fragments arranged by size:
4822 368 366 256 244 227 200 148
134 112 112 111 92 79 28 24
8 1 1
EcoRI G'AATT_C
Cuts at: 4642 5020 4642
Size: 378 6955
FspI TGC'GCA
Cuts at: 4092 5839 4092
Size: 1747 5586
HaeII r_GCGC'y
Cuts at: 1678 3558 6480 1678
Size: 1880 2922 2531
Fragments arranged by size:
2922 2531 1880
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2518 2550 3705
Size: 32 135 32 434 32 480 32 1155
Cuts at: 3705 3737 5974 6006 6048 6080 1373
Size: 32 2237 32 42 32 2626
Fragments arranged by size:
2626 2237 1155 480 434 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3781 4677 4923 669
Size: 988 281 1843 896 246 3079
Fragments arranged by size:
3079 1843 988 896 281 246
HindIII A'AGCT_T
Cuts at: 748 3737 748
Size: 2989 4344
HpaI GTT'AAC
Cuts at: 4923 4923
Size: 7333
KasI G'GCGC_C
Cuts at: 3554 3554
Size: 7333
KpnI G_GTAC'C
Cuts at: 4658 4658
Size: 7333
MfeI C'AATT_G
Cuts at: 4932 4932
Size: 7333
MluI A'CGCG_T
Cuts at: 7209 7209
Size: 7333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 3348 6447 6631 1305
Size: 870 1173 3099 184 2007
Fragments arranged by size:
3099 2007 1173 870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2755 3230 3278 4166 4196
Size: 54 1425 343 923 475 48 888 30
Cuts at: 4196 10
Size: 3147
Fragments arranged by size:
3147 1425 923 888 475 343 54 48
30
MslI CAynn'nnrTG
Cuts at: 518 1166 3771 4340 5291 5650 5809 518
Size: 648 2605 569 951 359 159 2042
Fragments arranged by size:
2605 2042 951 648 569 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 2409 2695 4045 4317 4594 4749 5426
Size: 782 21 286 1350 272 277 155 677
Cuts at: 5426 6574 6819 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1350 1148 782 677 286 277 272
245 155 21
NaeI GCC'GGC
Cuts at: 4490 4490
Size: 7333
NarI GG'CG_CC
Cuts at: 3555 3555
Size: 7333
NcoI C'CATG_G
Cuts at: 513 1065 2004 2478 2934 513
Size: 552 939 474 456 4912
Fragments arranged by size:
4912 939 552 474 456
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7333
NgoMIV G'CCGG_C
Cuts at: 4488 4488
Size: 7333
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7333
NotI GC'GGCC_GC
Cuts at: 4780 4780
Size: 7333
NspI r_CATG'y
Cuts at: 1132 1229 2116 2324 2875 4130 4156 4691 6236
Size: 97 887 208 551 1255 26 535 1545
Cuts at: 6236 1132
Size: 2229
Fragments arranged by size:
2229 1545 1255 887 551 535 208 97
26
PasI CC'CwG_GG
Cuts at: 1348 2107 2311 3401 3671 4073 1348
Size: 759 204 1090 270 402 4608
Fragments arranged by size:
4608 1090 759 402 270 204
PciI A'CATG_T
Cuts at: 6232 6232
Size: 7333
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2160 2940 4045 4317 1108
Size: 1052 780 1105 272 4124
Fragments arranged by size:
4124 1105 1052 780 272
PfoI T'CCnGG_A
Cuts at: 2348 4476 2348
Size: 2128 5205
PmeI GTTT'AAAC
Cuts at: 4637 4637
Size: 7333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 5402 5434 6915 6947 3104
Size: 32 2266 32 1481 32 3490
Fragments arranged by size:
3490 2266 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 2464 2986 3434 3934 4189 2440
Size: 24 522 448 500 255 5584
Fragments arranged by size:
5584 522 500 448 255 24
PshAI GACnn'nnGTC
Cuts at: 1613 2578 1613
Size: 965 6368
PsiI TTA'TAA
Cuts at: 4903 4903
Size: 7333
PspOMI G'GGCC_C
Cuts at: 2471 3065 3689 4068 4097 4416 2471
Size: 594 624 379 29 319 5388
Fragments arranged by size:
5388 624 594 379 319 29
PstI C_TGCA'G
Cuts at: 830 2676 3045 3627 4685 830
Size: 1846 369 582 1058 3478
Fragments arranged by size:
3478 1846 1058 582 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7333
PvuII CAG'CTG
Cuts at: 2695 4045 4594 2695
Size: 1350 549 5434
Fragments arranged by size:
5434 1350 549
SacI G_AGCT'C
Cuts at: 2550 2550
Size: 7333
SalI G'TCGA_C
Cuts at: 1936 3779 4675 1936
Size: 1843 896 4594
Fragments arranged by size:
4594 1843 896
SanDI GG'GwC_CC
Cuts at: 2986 2986
Size: 7333
SbfI CC_TGCA'GG
Cuts at: 3627 4685 3627
Size: 1058 6275
ScaI AGT'ACT
Cuts at: 2261 5581 7293 2261
Size: 3320 1712 2301
Fragments arranged by size:
3320 2301 1712
SexAI A'CCwGG_T
Cuts at: 2289 2289
Size: 7333
SfcI C'TryA_G
Cuts at: 826 1045 2672 3041 3520 3623 4411 4681 5816
Size: 219 1627 369 479 103 788 270 1135
Cuts at: 5816 6497 6688 826
Size: 681 191 1471
Fragments arranged by size:
1627 1471 1135 788 681 479 369 270
219 191 103
SfoI GGC'GCC
Cuts at: 3556 3556
Size: 7333
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7333
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7333
SmaI CCC'GGG
Cuts at: 1872 2375 2510 2999 4660 1872
Size: 503 135 489 1661 4545
Fragments arranged by size:
4545 1661 503 489 135
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3789 3929 5437 6338 6600 820
Size: 197 926 1846 140 1508 901 262 1553
Fragments arranged by size:
1846 1553 1508 926 901 262 197 140
SnaBI TAC'GTA
Cuts at: 493 5124 493
Size: 4631 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7333
SphI G_CATG'C
Cuts at: 1132 2116 2324 4156 4691 1132
Size: 984 208 1832 535 3774
Fragments arranged by size:
3774 1832 984 535 208
SrfI GCCC'GGGC
Cuts at: 2999 2999
Size: 7333
SspI AAT'ATT
Cuts at: 5 52 5257 5
Size: 47 5205 2081
Fragments arranged by size:
5205 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7333
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2467 2478 2934 2968 3016
Size: 552 939 243 220 11 456 34 48
Cuts at: 3016 3231 4772 513
Size: 215 1541 3074
Fragments arranged by size:
3074 1541 939 552 456 243 220 215
48 34 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 5360 5377 5530 5715 6160 325
Size: 1255 744 3036 17 153 185 445 1498
Fragments arranged by size:
3036 1498 1255 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4180 5579
Size: 274 80 33 51 1443 282 1921 1399
Cuts at: 5579 7291 96
Size: 1712 138
Fragments arranged by size:
1921 1712 1443 1399 282 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2646 2678 2777 2809 7293 7325 2646
Size: 32 99 32 4484 32 2654
Fragments arranged by size:
4484 2654 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2519 1316
Size: 1203 6130
XbaI T'CTAG_A
Cuts at: 4669 4669
Size: 7333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2699 2803 3156 4318 2699
Size: 104 353 1162 5714
Fragments arranged by size:
5714 1162 353 104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7333
XmaI C'CCGG_G
Cuts at: 1870 2373 2508 2997 4658 1870
Size: 503 135 489 1661 4545
Fragments arranged by size:
4545 1661 503 489 135
XmnI GAAnn'nnTTC
Cuts at: 3896 5462 3896
Size: 1566 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5141 276
Size: 53 83 186 936 3607 2468
Fragments arranged by size:
3607 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BfrBI BsiWI BsmBI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SapI SfiI SwaI