Restriction map for
pFN21AB2048
(Circular) (Six-base) MAPSORT of: pFN21AB2048.seq Check: 5298 from: 1 to: 7756
pFN21AB2048 7756 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5566 278
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4827 5099 1937
Size: 2890 272 4594
Fragments arranged by size:
4594 2890 272
Acc65I G'GTAC_C
Cuts at: 4836 5077 4836
Size: 241 7515
AclI AA'CG_TT
Cuts at: 1563 5883 6256 1563
Size: 4320 373 3063
Fragments arranged by size:
4320 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 4067 4187 4985 5819 7203 7471 1632
Size: 404 2031 120 798 834 1384 268 1917
Fragments arranged by size:
2031 1917 1384 834 798 404 268 120
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7559
AflIII A'CryG_T
Cuts at: 1203 2945 6655 7632 1203
Size: 1742 3710 977 1327
Fragments arranged by size:
3710 1742 1327 977
AgeI A'CCGG_T
Cuts at: 6587 6587
Size: 7756
AhdI GACnn_n'nnGTC
Cuts at: 6485 6485
Size: 7756
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7756
AlwNI CAG_nnn'CTG
Cuts at: 3241 3508 3566 5499 7071 3241
Size: 267 58 1933 1572 3926
Fragments arranged by size:
3926 1933 1572 267 58
ApaLI G'TGCA_C
Cuts at: 5813 6969 5813
Size: 1156 6600
ApoI r'AATT_y
Cuts at: 1493 2499 2828 5065 5291 5443 7552 1493
Size: 1006 329 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 1006 329 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6310 160
Size: 872 5278 1606
Fragments arranged by size:
5278 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
AvaI C'yCGr_G
Cuts at: 1870 1943 3457 3816 4690 5081 1870
Size: 73 1514 359 874 391 4545
Fragments arranged by size:
4545 1514 874 391 359 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3508 3541 3508
Size: 33 7723
BamHI G'GATC_C
Cuts at: 4215 5086 5453 4215
Size: 871 367 6518
Fragments arranged by size:
6518 871 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2802 3473 3542 4836 5077
Size: 325 217 627 1015 671 69 1294 241
Cuts at: 5077 6532 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1294 1015 671 627 325 241
217 69
BanII G_rGCy'C
Cuts at: 1391 3278 3464 5075 1391
Size: 1887 186 1611 4072
Fragments arranged by size:
4072 1887 1611 186
BbeI G_GCGC'C
Cuts at: 3546 3546
Size: 7756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2786 928
Size: 971 887 5898
Fragments arranged by size:
5898 971 887
BbvCI CC'TCA_GC
Cuts at: 3809 3809
Size: 7756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2311 2345 4860 4894 5947 5981 1903
Size: 34 374 34 2515 34 1053 34 3678
Fragments arranged by size:
3678 2515 1053 374 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5648 6858 5648
Size: 1210 6546
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6367 136
Size: 107 122 71 631 605 4695 1525
Fragments arranged by size:
4695 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4482 7751 4482
Size: 3269 4487
BlpI GC'TnA_GC
Cuts at: 2687 3277 4157 5173 2687
Size: 590 880 1016 5270
Fragments arranged by size:
5270 1016 880 590
Bme1580I G_kGCm'C
Cuts at: 1790 2805 3476 5817 6973 1790
Size: 1015 671 2341 1156 2573
Fragments arranged by size:
2573 2341 1156 1015 671
BmgBI CAC'GTC
Cuts at: 1318 4243 1318
Size: 2925 4831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4502 4901
Size: 416 53 83 399 24 2009 1076 399
Cuts at: 4901 5487 6445 442
Size: 586 958 1753
Fragments arranged by size:
2009 1753 1076 958 586 416 399 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2444 1056
Size: 1388 6368
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 7756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2127 2135 2320 3505 3848 3972 4070
Size: 876 12 8 185 1185 343 124 98
Cuts at: 4070 6416 1239
Size: 2346 2579
Fragments arranged by size:
2579 2346 1185 876 343 185 124 98
12 8
Bpu10I CC'TnA_GC
Cuts at: 3101 3809 3867 4651 3101
Size: 708 58 784 6206
Fragments arranged by size:
6206 784 708 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2562 3194 3917 3923 5881 6746 7044 2562
Size: 632 723 6 1958 865 298 3274
Fragments arranged by size:
3274 1958 865 723 632 298 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2427 2624 3813 4736 6419 882
Size: 617 928 197 1189 923 1683 2219
Fragments arranged by size:
2219 1683 1189 928 923 617 197
BsaAI yAC'GTr
Cuts at: 493 1204 2307 5547 493
Size: 711 1103 3240 2702
Fragments arranged by size:
3240 2702 1103 711
BsaBI GATnn'nnATC
Cuts at: 7750 7750
Size: 7756
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3543 4638 5563 5945
Size: 53 83 186 936 2010 1095 925 382
Cuts at: 5945 6580 275
Size: 635 1451
Fragments arranged by size:
2010 1451 1095 936 925 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2385 2805 4897 6189 6587 6861 7008 7419 2385
Size: 420 2092 1292 398 274 147 411 2722
Fragments arranged by size:
2722 2092 1292 420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3619 3649 4004 4034 1178
Size: 30 165 30 2216 30 355 30 4900
Fragments arranged by size:
4900 2216 355 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3122 3125 4295 4811 1186
Size: 1936 3 1170 516 4131
Fragments arranged by size:
4131 1936 1170 516 3
BseYI C'CCAG_C
Cuts at: 2318 3025 3171 6959 7455 2318
Size: 707 146 3788 496 2619
Fragments arranged by size:
3788 2619 707 496 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4014 4626 4754 1163
Size: 573 2278 612 128 4165
Fragments arranged by size:
4165 2278 612 573 128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2960 5206 5967 6995 1540
Size: 275 186 959 2246 761 1028 2301
Fragments arranged by size:
2301 2246 1028 959 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3742 4652 5817 5902 6973 3742
Size: 910 1165 85 1071 4525
Fragments arranged by size:
4525 1165 1071 910 85
BsmI GAATG_Cn'
Cuts at: 2485 3062 3123 3434 3628 4076 5267 5360 2485
Size: 577 61 311 194 448 1191 93 4881
Fragments arranged by size:
4881 1191 577 448 311 194 93 61
BsmBI CGTCTCn'nnnn_
Cuts at: 3962 4190 4307 3962
Size: 228 117 7411
Fragments arranged by size:
7411 228 117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2805 3278 3464 3476 3742 4652 5075
Size: 399 1015 473 186 12 266 910 423
Cuts at: 5075 5817 5902 6973 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1015 910 742 473 423 399
266 186 85 12
BspEI T'CCGG_A
Cuts at: 2385 7419 2385
Size: 5034 2722
BspHI T'CATG_A
Cuts at: 3159 5645 7375 3159
Size: 2486 1730 3540
Fragments arranged by size:
3540 2486 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3598 5111 844
Size: 2754 1513 3489
Fragments arranged by size:
3489 2754 1513
BsrBI CCG'CTC
Cuts at: 1394 1823 2925 3718 5643 1394
Size: 429 1102 793 1925 3507
Fragments arranged by size:
3507 1925 1102 793 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4345 6251 6425 65
Size: 1183 3097 1906 174 1396
Fragments arranged by size:
3097 1906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6400 6587 1700
Size: 110 4590 187 2869
Fragments arranged by size:
4590 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4110 96
Size: 4014 3742
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7756
BssSI C'ACGA_G
Cuts at: 4802 5816 6828 4802
Size: 1014 1012 5730
Fragments arranged by size:
5730 1014 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7756
BstBI TT'CG_AA
Cuts at: 6560 6560
Size: 7756
BstEII G'GTnAC_C
Cuts at: 1184 2694 1184
Size: 1510 6246
BstXI CCAn_nnnn'nTGG
Cuts at: 3271 3271
Size: 7756
BstYI r'GATC_y
Cuts at: 1972 4215 4482 5086 5453 5838 5855 7307 7393
Size: 2243 267 604 367 385 17 1452 86
Cuts at: 7393 7405 7751 1972
Size: 12 346 1977
Fragments arranged by size:
2243 1977 1452 604 385 367 346 267
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5045 5045
Size: 7756
BtgI C'CryG_G
Cuts at: 513 1065 3429 513
Size: 552 2364 4840
Fragments arranged by size:
4840 2364 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2415 3861 487
Size: 683 56 1189 1446 4382
Fragments arranged by size:
4382 1446 1189 683 56
BtsI GCAGTG_nn'
Cuts at: 2369 2748 3844 4221 5272 6066 6086 2369
Size: 379 1096 377 1051 794 20 4039
Fragments arranged by size:
4039 1096 1051 794 379 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4920 4955 529
Size: 35 4356 35 3330
Fragments arranged by size:
4356 3330 35 35
DraI TTT'AAA
Cuts at: 5060 5060
Size: 7756
DraIII CAC_nnn'GTG
Cuts at: 3737 5022 3737
Size: 1285 6471
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 809
Size: 507 5447 1802
Fragments arranged by size:
5447 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2957 3373 5203
Size: 54 1425 50 275 18 1127 416 1830
Cuts at: 5203 6092 7721 8
Size: 889 1629 43
Fragments arranged by size:
1830 1629 1425 1127 889 416 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2957 5203 1537
Size: 275 1145 2246 4090
Fragments arranged by size:
4090 2246 1145 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3110 3245 3490 4428 4718 4946 5018 5686
Size: 1757 135 245 938 290 228 72 668
Cuts at: 5686 1353
Size: 3423
Fragments arranged by size:
3423 1757 938 668 290 245 228 135
72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6343 6717 6863 387
Size: 1064 138 4754 374 146 1280
Fragments arranged by size:
4754 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2115 2127 2135 2320 3505 3848
Size: 393 404 79 12 8 185 1185 343
Cuts at: 3848 3972 4067 4070 4187 4985 5819 6416 7203
Size: 124 95 3 117 798 834 597 787
Cuts at: 7203 7471 1239
Size: 268 1524
Fragments arranged by size:
1524 1185 834 798 787 597 404 393
343 268 185 124 117 95 79 12
8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 3104 2153
Size: 951 6805
EcoO109I rG'GnC_Cy
Cuts at: 2551 2744 3813 2551
Size: 193 1069 6494
Fragments arranged by size:
6494 1069 193
EcoRI G'AATT_C
Cuts at: 5065 5443 5065
Size: 378 7378
EcoRV GAT'ATC
Cuts at: 3325 3325
Size: 7756
FspI TGC'GCA
Cuts at: 6262 6262
Size: 7756
HaeII r_GCGC'y
Cuts at: 1678 3546 4373 5011 6903 1678
Size: 1868 827 638 1892 2531
Fragments arranged by size:
2531 1892 1868 827 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2191 2223 6397 6429 6471
Size: 32 135 32 619 32 4174 32 42
Cuts at: 6471 6503 1373
Size: 32 2626
Fragments arranged by size:
4174 2626 619 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2032 2544 3304 3663 4932 5100
Size: 988 281 94 512 760 359 1269 168
Cuts at: 5100 5346 669
Size: 246 3079
Fragments arranged by size:
3079 1269 988 760 512 359 281 246
168 94
HindIII A'AGCT_T
Cuts at: 748 2345 748
Size: 1597 6159
HpaI GTT'AAC
Cuts at: 3663 5346 3663
Size: 1683 6073
KasI G'GCGC_C
Cuts at: 3542 3542
Size: 7756
KpnI G_GTAC'C
Cuts at: 4840 5081 4840
Size: 241 7515
MfeI C'AATT_G
Cuts at: 5355 5355
Size: 7756
MluI A'CGCG_T
Cuts at: 7632 7632
Size: 7756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 3788 4710 6870 7054 1305
Size: 1302 1181 922 2160 184 2007
Fragments arranged by size:
2160 2007 1302 1181 922 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5934
Fragments arranged by size:
5934 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3965 4118 5714 6073 6232 518
Size: 648 2799 153 1596 359 159 2042
Fragments arranged by size:
2799 2042 1596 648 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2691 2962 3241 3409 3770 3992 4236 4379
Size: 1085 271 279 168 361 222 244 143
Cuts at: 4379 5172 5849 6997 7242 1606
Size: 793 677 1148 245 2120
Fragments arranged by size:
2120 1148 1085 793 677 361 279 271
245 244 222 168 143
NarI GG'CG_CC
Cuts at: 3543 3543
Size: 7756
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7756
NheI G'CTAG_C
Cuts at: 1052 2440 1052
Size: 1388 6368
NotI GC'GGCC_GC
Cuts at: 5203 5203
Size: 7756
NspI r_CATG'y
Cuts at: 1132 1229 2220 2949 5114 6659 1132
Size: 97 991 729 2165 1545 2229
Fragments arranged by size:
2229 2165 1545 991 729 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7756
PciI A'CATG_T
Cuts at: 2945 6655 2945
Size: 3710 4046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7756
PfoI T'CCnGG_A
Cuts at: 2151 3008 3825 2151
Size: 857 817 6082
Fragments arranged by size:
6082 857 817
PmeI GTTT'AAAC
Cuts at: 5060 5060
Size: 7756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5825 5857 7338 7370 5825
Size: 32 1481 32 6211
Fragments arranged by size:
6211 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2551
Size: 7756
PshAI GACnn'nnGTC
Cuts at: 1613 2027 3954 1613
Size: 414 1927 5415
Fragments arranged by size:
5415 1927 414
PsiI TTA'TAA
Cuts at: 5326 5326
Size: 7756
PstI C_TGCA'G
Cuts at: 830 3595 4384 4794 5108 830
Size: 2765 789 410 314 3478
Fragments arranged by size:
3478 2765 789 410 314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7756
PvuII CAG'CTG
Cuts at: 2691 3241 3409 3770 3992 4236 2691
Size: 550 168 361 222 244 6211
Fragments arranged by size:
6211 550 361 244 222 168
SalI G'TCGA_C
Cuts at: 1936 5098 1936
Size: 3162 4594
SanDI GG'GwC_CC
Cuts at: 2551 2551
Size: 7756
SapI GCTCTTCn'nnn_
Cuts at: 3110 4718 3110
Size: 1608 6148
SbfI CC_TGCA'GG
Cuts at: 3595 5108 3595
Size: 1513 6243
ScaI AGT'ACT
Cuts at: 6004 7716 6004
Size: 1712 6044
SexAI A'CCwGG_T
Cuts at: 2712 3733 4569 4767 2712
Size: 1021 836 198 5701
Fragments arranged by size:
5701 1021 836 198
SfcI C'TryA_G
Cuts at: 826 1045 3591 4380 4790 5104 6239 6920 7111
Size: 219 2546 789 410 314 1135 681 191
Cuts at: 7111 826
Size: 1471
Fragments arranged by size:
2546 1471 1135 789 681 410 314 219
191
SfoI GGC'GCC
Cuts at: 3544 3544
Size: 7756
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7756
SmaI CCC'GGG
Cuts at: 1872 5083 1872
Size: 3211 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2577 3209 3896 3902 5860 6761
Size: 197 926 634 632 687 6 1958 901
Cuts at: 6761 7023 820
Size: 262 1553
Fragments arranged by size:
1958 1553 926 901 687 634 632 262
197 6
SnaBI TAC'GTA
Cuts at: 493 2307 5547 493
Size: 1814 3240 2702
Fragments arranged by size:
3240 2702 1814
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7756
SphI G_CATG'C
Cuts at: 1132 5114 1132
Size: 3982 3774
SspI AAT'ATT
Cuts at: 5 52 2179 5680 5
Size: 47 2127 3501 2081
Fragments arranged by size:
3501 2127 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7756
StyI C'CwwG_G
Cuts at: 513 1065 5195 513
Size: 552 4130 3074
Fragments arranged by size:
4130 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3179 5783 5800 5953 6138 6583 325
Size: 1255 1599 2604 17 153 185 445 1498
Fragments arranged by size:
2604 1599 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4110 6002 7714
Size: 274 80 33 51 1443 2133 1892 1712
Cuts at: 7714 96
Size: 138
Fragments arranged by size:
2133 1892 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4004 4036 7716 7748 4004
Size: 32 3680 32 4012
Fragments arranged by size:
4012 3680 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3670 1316
Size: 2354 5402
XbaI T'CTAG_A
Cuts at: 5092 5092
Size: 7756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3086 3137 4567 3086
Size: 51 1430 6275
Fragments arranged by size:
6275 1430 51
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7756
XmaI C'CCGG_G
Cuts at: 1870 5081 1870
Size: 3211 4545
XmnI GAAnn'nnTTC
Cuts at: 2178 5885 2178
Size: 3707 4049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5564 276
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BstZ17I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SfiI SrfI SwaI