Restriction map for
pFN21AB1986
(Circular) (Six-base) MAPSORT of: pFN21AB1986.seq Check: 9496 from: 1 to: 8014
pFN21AB1986 8014 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5824 278
Size: 53 83 186 936 4288 2468
Fragments arranged by size:
4288 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3249 5357 1937
Size: 1312 2108 4594
Fragments arranged by size:
4594 2108 1312
Acc65I G'GTAC_C
Cuts at: 5335 5335
Size: 8014
AclI AA'CG_TT
Cuts at: 1563 4224 6141 6514 1563
Size: 2661 1917 373 3063
Fragments arranged by size:
3063 2661 1917 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 3948 4517 4731 6077 7461 7729 1632
Size: 1661 655 569 214 1346 1384 268 1917
Fragments arranged by size:
1917 1661 1384 1346 655 569 268 214
AflII C'TTAA_G
Cuts at: 820 1017 4325 820
Size: 197 3308 4509
Fragments arranged by size:
4509 3308 197
AflIII A'CryG_T
Cuts at: 1203 4723 6913 7890 1203
Size: 3520 2190 977 1327
Fragments arranged by size:
3520 2190 1327 977
AgeI A'CCGG_T
Cuts at: 6845 6845
Size: 8014
AhdI GACnn_n'nnGTC
Cuts at: 6743 6743
Size: 8014
AleI CACnn'nnGTG
Cuts at: 1166 3405 1166
Size: 2239 5775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4680 1184
Size: 3496 4518
AlwNI CAG_nnn'CTG
Cuts at: 4174 5757 7329 4174
Size: 1583 1572 4859
Fragments arranged by size:
4859 1583 1572
ApaLI G'TGCA_C
Cuts at: 6071 7227 6071
Size: 1156 6858
ApoI r'AATT_y
Cuts at: 1493 2302 3080 3545 3666 4054 4146 4703 4755
Size: 809 778 465 121 388 92 557 52
Cuts at: 4755 4820 5323 5549 5701 7810 1493
Size: 65 503 226 152 2109 1697
Fragments arranged by size:
2109 1697 809 778 557 503 465 388
226 152 121 92 65 52
AseI AT'TA_AT
Cuts at: 160 1032 6568 160
Size: 872 5536 1606
Fragments arranged by size:
5536 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8014
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 4887 5339 1870
Size: 73 72 2872 452 4545
Fragments arranged by size:
4545 2872 452 73 72
BamHI G'GATC_C
Cuts at: 3415 4153 5344 5711 3415
Size: 738 1191 367 5718
Fragments arranged by size:
5718 1191 738 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5335 6790 618
Size: 325 217 627 3548 1455 1842
Fragments arranged by size:
3548 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3751 4382 5333 1391
Size: 2360 631 951 4072
Fragments arranged by size:
4072 2360 951 631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2502 4017 4115 4218 4251 5084 928
Size: 971 603 1515 98 103 33 833 3858
Fragments arranged by size:
3858 1515 971 833 603 103 98 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6205 6239 1903
Size: 34 4268 34 3678
Fragments arranged by size:
4268 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3786 4424 5906 7116 3786
Size: 638 1482 1210 4684
Fragments arranged by size:
4684 1482 1210 638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8014
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4046 6625 136
Size: 107 122 71 631 605 2374 2579 1525
Fragments arranged by size:
2579 2374 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3115 4030 8009 3115
Size: 915 3979 3120
Fragments arranged by size:
3979 3120 915
BlpI GC'TnA_GC
Cuts at: 2274 5431 2274
Size: 3157 4857
Bme1580I G_kGCm'C
Cuts at: 1790 6075 7231 1790
Size: 4285 1156 2573
Fragments arranged by size:
4285 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4122 4573 5745
Size: 416 53 83 399 24 2705 451 1172
Cuts at: 5745 6703 442
Size: 958 1753
Fragments arranged by size:
2705 1753 1172 958 451 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4253 6674 1239
Size: 3014 2421 2579
Fragments arranged by size:
3014 2579 2421
Bpu10I CC'TnA_GC
Cuts at: 4293 4293
Size: 8014
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2229 3552 3637 4552 4745 6139 7004 7302 2229
Size: 1323 85 915 193 1394 865 298 2941
Fragments arranged by size:
2941 1394 1323 915 865 298 193 85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5229 6677 882
Size: 617 3730 1448 2219
Fragments arranged by size:
3730 2219 1448 617
BsaAI yAC'GTr
Cuts at: 493 1204 4726 5805 493
Size: 711 3522 1079 2702
Fragments arranged by size:
3522 2702 1079 711
BsaBI GATnn'nnATC
Cuts at: 8008 8008
Size: 8014
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 5821 6203 6838
Size: 53 83 186 936 494 3794 382 635
Cuts at: 6838 275
Size: 1451
Fragments arranged by size:
3794 1451 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2605 6447 6845 7119 7266 7677 2605
Size: 3842 398 274 147 411 2942
Fragments arranged by size:
3842 2942 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7789
Fragments arranged by size:
7789 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3741 3750 3759 3825 3855 3870 1186
Size: 2555 9 9 66 30 15 5330
Fragments arranged by size:
5330 2555 66 30 15 9 9
BseYI C'CCAG_C
Cuts at: 2613 7217 7713 2613
Size: 4604 496 2914
Fragments arranged by size:
4604 2914 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5244 1163
Size: 573 3508 3933
Fragments arranged by size:
3933 3508 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2540 5464 6225 7253 1540
Size: 275 186 539 2924 761 1028 2301
Fragments arranged by size:
2924 2301 1028 761 539 275 186
BsiHKAI G_wGCw'C
Cuts at: 3751 4382 6075 6160 7231 3751
Size: 631 1693 85 1071 4534
Fragments arranged by size:
4534 1693 1071 631 85
BsmI GAATG_Cn'
Cuts at: 2504 5228 5525 5618 2504
Size: 2724 297 93 4900
Fragments arranged by size:
4900 2724 297 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3212 4409 3212
Size: 1197 6817
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3751 4382 5333 6075 6160 7231 1391
Size: 399 1961 631 951 742 85 1071 2174
Fragments arranged by size:
2174 1961 1071 951 742 631 399 85
BspEI T'CCGG_A
Cuts at: 7677 7677
Size: 8014
BspHI T'CATG_A
Cuts at: 2527 5903 7633 2527
Size: 3376 1730 2908
Fragments arranged by size:
3376 2908 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5369 844
Size: 4525 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2036 5901 1394
Size: 429 213 3865 3507
Fragments arranged by size:
3865 3507 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 3692 6509 6683 65
Size: 1183 2444 2817 174 1396
Fragments arranged by size:
2817 2444 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6658 6845 1700
Size: 110 4848 187 2869
Fragments arranged by size:
4848 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8014
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8014
BssSI C'ACGA_G
Cuts at: 6074 7086 6074
Size: 1012 7002
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5096 794
Size: 4302 3712
BstBI TT'CG_AA
Cuts at: 3061 3631 6818 3061
Size: 570 3187 4257
Fragments arranged by size:
4257 3187 570
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8014
BstXI CCAn_nnnn'nTGG
Cuts at: 4186 4186
Size: 8014
BstYI r'GATC_y
Cuts at: 1972 2326 3115 3415 4030 4153 5344 5711 6096
Size: 354 789 300 615 123 1191 367 385
Cuts at: 6096 6113 7565 7651 7663 8009 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1191 789 615 385 367 354
346 300 123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2582 2582
Size: 8014
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6523
Fragments arranged by size:
6523 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2267 487
Size: 683 56 1041 6234
Fragments arranged by size:
6234 1041 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 3279 3400 3412 5046 5530 6324 6344 2439
Size: 840 121 12 1634 484 794 20 4109
Fragments arranged by size:
4109 1634 840 794 484 121 20 12
ClaI AT'CG_AT
Cuts at: 1554 3450 1554
Size: 1896 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7979
DraI TTT'AAA
Cuts at: 5318 5318
Size: 8014
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7021 809
Size: 507 5705 1802
Fragments arranged by size:
5705 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2629 5461 6350
Size: 54 1425 50 275 18 799 2832 889
Cuts at: 6350 7979 8
Size: 1629 43
Fragments arranged by size:
2832 1629 1425 889 799 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5461 1537
Size: 275 3649 4090
Fragments arranged by size:
4090 3649 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2361 2880 3759 3935 4986 5056 5067
Size: 750 258 519 879 176 1051 70 11
Cuts at: 5067 5944 1353
Size: 877 3423
Fragments arranged by size:
3423 1051 879 877 750 519 258 176
70 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6601 6975 7121 387
Size: 1064 138 5012 374 146 1280
Fragments arranged by size:
5012 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3749 4380 3749
Size: 631 7383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3293 3948 4253 4517 4731 6077 6674
Size: 393 1661 655 305 264 214 1346 597
Cuts at: 6674 7461 7729 1239
Size: 787 268 1524
Fragments arranged by size:
1661 1524 1346 787 655 597 393 305
268 264 214
EcoNI CCTnn'n_nnAGG
Cuts at: 3870 3870
Size: 8014
EcoO109I rG'GnC_Cy
Cuts at: 3674 3830 3674
Size: 156 7858
EcoRI G'AATT_C
Cuts at: 3080 3545 3666 5323 5701 3080
Size: 465 121 1657 378 5393
Fragments arranged by size:
5393 1657 465 378 121
EcoRV GAT'ATC
Cuts at: 4696 4696
Size: 8014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3593 4618 3593
Size: 1025 6989
FspI TGC'GCA
Cuts at: 6520 6520
Size: 8014
HaeII r_GCGC'y
Cuts at: 1678 7161 1678
Size: 5483 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 3432 3464 4581
Size: 32 135 32 707 32 1121 32 1117
Cuts at: 4581 4613 4645 5182 5214 6655 6687 6729 6761
Size: 32 32 537 32 1441 32 42 32
Cuts at: 6761 1373
Size: 2626
Fragments arranged by size:
2626 1441 1121 1117 707 537 135 42
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 669 1657 1938 2620 5358 5604 669
Size: 988 281 682 2738 246 3079
Fragments arranged by size:
3079 2738 988 682 281 246
HindIII A'AGCT_T
Cuts at: 748 2669 4510 748
Size: 1921 1841 4252
Fragments arranged by size:
4252 1921 1841
HpaI GTT'AAC
Cuts at: 2620 5604 2620
Size: 2984 5030
KpnI G_GTAC'C
Cuts at: 5339 5339
Size: 8014
MfeI C'AATT_G
Cuts at: 2814 2961 3949 5613 2814
Size: 147 988 1664 5215
Fragments arranged by size:
5215 1664 988 147
MluI A'CGCG_T
Cuts at: 7890 7890
Size: 8014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2437 2860 4525 7128 7312 1305
Size: 1132 423 1665 2603 184 2007
Fragments arranged by size:
2603 2007 1665 1132 423 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2631 10
Size: 54 1425 343 799 5393
Fragments arranged by size:
5393 1425 799 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3405 5280 5972 6331 6490 518
Size: 648 2239 1875 692 359 159 2042
Fragments arranged by size:
2239 2042 1875 692 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2613 4634 5430 6107 7255 7500 1606
Size: 1007 2021 796 677 1148 245 2120
Fragments arranged by size:
2120 2021 1148 1007 796 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6523
Fragments arranged by size:
6523 939 552
NdeI CA'TA_TG
Cuts at: 387 4207 387
Size: 3820 4194
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8014
NotI GC'GGCC_GC
Cuts at: 5461 5461
Size: 8014
NruI TCG'CGA
Cuts at: 2252 2252
Size: 8014
NspI r_CATG'y
Cuts at: 1132 1229 3016 4204 4252 5372 6917 1132
Size: 97 1787 1188 48 1120 1545 2229
Fragments arranged by size:
2229 1787 1545 1188 1120 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8014
PciI A'CATG_T
Cuts at: 6913 6913
Size: 8014
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3851 4585 1108
Size: 2743 734 4537
Fragments arranged by size:
4537 2743 734
PmeI GTTT'AAAC
Cuts at: 5318 5318
Size: 8014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6083 6115 7596 7628 4550
Size: 32 1501 32 1481 32 4936
Fragments arranged by size:
4936 1501 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3674 3830 3674
Size: 156 7858
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8014
PsiI TTA'TAA
Cuts at: 3023 3363 5584 3023
Size: 340 2221 5453
Fragments arranged by size:
5453 2221 340
PstI C_TGCA'G
Cuts at: 830 2272 3407 3520 3685 5366 830
Size: 1442 1135 113 165 1681 3478
Fragments arranged by size:
3478 1681 1442 1135 165 113
PvuI CG_AT'CG
Cuts at: 2001 2540 2001
Size: 539 7475
PvuII CAG'CTG
Cuts at: 2613 2613
Size: 8014
SacI G_AGCT'C
Cuts at: 3751 4382 3751
Size: 631 7383
SalI G'TCGA_C
Cuts at: 1936 5356 1936
Size: 3420 4594
SbfI CC_TGCA'GG
Cuts at: 5366 5366
Size: 8014
ScaI AGT'ACT
Cuts at: 3648 6262 7974 3648
Size: 2614 1712 3688
Fragments arranged by size:
3688 2614 1712
SfcI C'TryA_G
Cuts at: 826 1045 2268 2451 3403 3516 3681 5362 6497
Size: 219 1223 183 952 113 165 1681 1135
Cuts at: 6497 7178 7369 826
Size: 681 191 1471
Fragments arranged by size:
1681 1471 1223 1135 952 681 219 191
183 165 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8014
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8014
SmaI CCC'GGG
Cuts at: 1872 2017 5341 1872
Size: 145 3324 4545
Fragments arranged by size:
4545 3324 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2244 3567 3652 4325 4567 4760
Size: 197 926 301 1323 85 673 242 193
Cuts at: 4760 6118 7019 7281 820
Size: 1358 901 262 1553
Fragments arranged by size:
1553 1358 1323 926 901 673 301 262
242 197 193 85
SnaBI TAC'GTA
Cuts at: 493 5805 493
Size: 5312 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8014
SphI G_CATG'C
Cuts at: 1132 3016 4204 5372 1132
Size: 1884 1188 1168 3774
Fragments arranged by size:
3774 1884 1188 1168
SspI AAT'ATT
Cuts at: 5 52 2698 3359 5938 5
Size: 47 2646 661 2579 2081
Fragments arranged by size:
2646 2579 2081 661 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8014
StyI C'CwwG_G
Cuts at: 513 1065 2004 3850 5255 5453 513
Size: 552 939 1846 1405 198 3074
Fragments arranged by size:
3074 1846 1405 939 552 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2190 6041 6058 6211 6396 6841 325
Size: 1255 610 3851 17 153 185 445 1498
Fragments arranged by size:
3851 1498 1255 610 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3646 6260 7972
Size: 274 80 33 51 1443 1669 2614 1712
Cuts at: 7972 96
Size: 138
Fragments arranged by size:
2614 1712 1669 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7974 8006 7974
Size: 32 7982
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8014
XbaI T'CTAG_A
Cuts at: 2896 5203 5350 2896
Size: 2307 147 5560
Fragments arranged by size:
5560 2307 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 2639 2413
Size: 226 7788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8014
XmaI C'CCGG_G
Cuts at: 1870 2015 5339 1870
Size: 145 3324 4545
Fragments arranged by size:
4545 3324 145
XmnI GAAnn'nnTTC
Cuts at: 2523 2670 3080 3989 6143 2523
Size: 147 410 909 2154 4394
Fragments arranged by size:
4394 2154 909 410 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5822 276
Size: 53 83 186 936 4288 2468
Fragments arranged by size:
4288 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI