Restriction map for
pFN21AB1487
(Circular) (Six-base) MAPSORT of: pFN21AB1487.seq Check: 6339 from: 1 to: 10444
pFN21AB1487 10444 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3692 3758 3766 4094 7652 7685 3692
Size: 66 8 328 3558 33 6451
Fragments arranged by size:
6451 3558 328 66 33 8
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8254 278
Size: 53 83 186 936 6718 2468
Fragments arranged by size:
6718 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2315 7787 1937
Size: 378 5472 4594
Fragments arranged by size:
5472 4594 378
Acc65I G'GTAC_C
Cuts at: 7765 7765
Size: 10444
AclI AA'CG_TT
Cuts at: 1563 7020 8571 8944 1563
Size: 5457 1551 373 3063
Fragments arranged by size:
5457 3063 1551 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2427 3002 3354 3584 3981 4301 4643 4769
Size: 795 575 352 230 397 320 342 126
Cuts at: 4769 5046 5129 5165 6309 6878 6915 7124 7130
Size: 277 83 36 1144 569 37 209 6
Cuts at: 7130 7389 8507 9891 10159 1632
Size: 259 1118 1384 268 1917
Fragments arranged by size:
1917 1384 1144 1118 795 575 569 397
352 342 320 277 268 259 230 209
126 83 37 36 6
AfeI AGC'GCT
Cuts at: 4105 4105
Size: 10444
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10247
AflIII A'CryG_T
Cuts at: 1203 2697 4329 6228 6933 9343 10320 1203
Size: 1494 1632 1899 705 2410 977 1327
Fragments arranged by size:
2410 1899 1632 1494 1327 977 705
AgeI A'CCGG_T
Cuts at: 9275 9275
Size: 10444
AhdI GACnn_n'nnGTC
Cuts at: 9173 9173
Size: 10444
AleI CACnn'nnGTG
Cuts at: 1166 3362 5984 1166
Size: 2196 2622 5626
Fragments arranged by size:
5626 2622 2196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2631 5363 1184
Size: 1447 2732 6265
Fragments arranged by size:
6265 2732 1447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2736 2768 5168 5200 2736
Size: 32 2400 32 7980
Fragments arranged by size:
7980 2400 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2656 3792 5314 6184 8187 9759 2656
Size: 1136 1522 870 2003 1572 3341
Fragments arranged by size:
3341 2003 1572 1522 1136 870
ApaI G_GGCC'C
Cuts at: 5070 5070
Size: 10444
ApaLI G'TGCA_C
Cuts at: 4259 4838 6749 8501 9657 4259
Size: 579 1911 1752 1156 5046
Fragments arranged by size:
5046 1911 1752 1156 579
ApoI r'AATT_y
Cuts at: 1493 3248 6425 6881 7753 7979 8131 10240 1493
Size: 1755 3177 456 872 226 152 2109 1697
Fragments arranged by size:
3177 2109 1755 1697 872 456 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8998 160
Size: 872 7966 1606
Fragments arranged by size:
7966 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10444
AvaI C'yCGr_G
Cuts at: 1870 1943 6338 7514 7769 1870
Size: 73 4395 1176 255 4545
Fragments arranged by size:
4545 4395 1176 255 73
BamHI G'GATC_C
Cuts at: 2062 7774 8141 2062
Size: 5712 367 4365
Fragments arranged by size:
5712 4365 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3356 3740 4157 4915 6797
Size: 325 217 627 1569 384 417 758 1882
Cuts at: 6797 6872 7765 9220 618
Size: 75 893 1455 1842
Fragments arranged by size:
1882 1842 1569 1455 893 758 627 417
384 325 217 75
BanII G_rGCy'C
Cuts at: 1391 2190 3708 4459 5070 5630 6041 6339 6915
Size: 799 1518 751 611 560 411 298 576
Cuts at: 6915 7305 7692 7763 1391
Size: 390 387 71 4072
Fragments arranged by size:
4072 1518 799 751 611 576 560 411
390 387 298 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3029 4602 4746 5361 7279 928
Size: 971 1130 1573 144 615 1918 4093
Fragments arranged by size:
4093 1918 1573 1130 971 615 144
BbvCI CC'TCA_GC
Cuts at: 2946 7027 7404 2946
Size: 4081 377 5986
Fragments arranged by size:
5986 4081 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2529 2563 8635 8669 1903
Size: 34 592 34 6072 34 3678
Fragments arranged by size:
6072 3678 592 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 7496 8336 9546 4058
Size: 3438 840 1210 4956
Fragments arranged by size:
4956 3438 1210 840
BclI T'GATC_A
Cuts at: 1548 6900 1548
Size: 5352 5092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3698 4561 5065
Size: 107 122 71 631 605 2026 863 504
Cuts at: 5065 9055 136
Size: 3990 1525
Fragments arranged by size:
3990 2026 1525 863 631 605 504 122
107 71
BglII A'GATC_T
Cuts at: 6111 6708 7065 10439 6111
Size: 597 357 3374 6116
Fragments arranged by size:
6116 3374 597 357
BlpI GC'TnA_GC
Cuts at: 3702 7861 3702
Size: 4159 6285
Bme1580I G_kGCm'C
Cuts at: 1790 2483 2620 3633 4079 4263 4842 5070 6753
Size: 693 137 1013 446 184 579 228 1683
Cuts at: 6753 6800 8505 9661 1790
Size: 47 1705 1156 2573
Fragments arranged by size:
2573 1705 1683 1156 1013 693 579 446
228 184 137 47
BmgBI CAC'GTC
Cuts at: 1318 2686 4330 4609 6135 1318
Size: 1368 1644 279 1526 5627
Fragments arranged by size:
5627 1644 1526 1368 279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3620 8175 9133
Size: 416 53 83 399 24 2203 4555 958
Cuts at: 9133 442
Size: 1753
Fragments arranged by size:
4555 2203 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5193 1056
Size: 4137 6307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3144 4047 3144
Size: 903 9541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2881 2888 2990 3126 3538 4128 5339 5559
Size: 1642 7 102 136 412 590 1211 220
Cuts at: 5559 6638 6860 9104 1239
Size: 1079 222 2244 2579
Fragments arranged by size:
2579 2244 1642 1211 1079 590 412 222
220 136 102 7
Bpu10I CC'TnA_GC
Cuts at: 2946 5045 6561 7027 7404 2946
Size: 2099 1516 466 377 5986
Fragments arranged by size:
5986 2099 1516 466 377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2573 4376 5189 5402 6746 7412 8569 9434
Size: 520 1803 813 213 1344 666 1157 865
Cuts at: 9434 9732 2053
Size: 298 2765
Fragments arranged by size:
2765 1803 1344 1157 865 813 666 520
298 213
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 4692 5000 5096 5132 6858 9107
Size: 617 948 2245 308 96 36 1726 2249
Cuts at: 9107 882
Size: 2219
Fragments arranged by size:
2249 2245 2219 1726 948 617 308 96
36
BsaAI yAC'GTr
Cuts at: 493 1204 2700 4080 6218 8235 493
Size: 711 1496 1380 2138 2017 2702
Fragments arranged by size:
2702 2138 2017 1496 1380 711
BsaBI GATnn'nnATC
Cuts at: 6415 6536 10438 6415
Size: 121 3902 6421
Fragments arranged by size:
6421 3902 121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8251 8633 9268 275
Size: 53 83 186 936 6718 382 635 1451
Fragments arranged by size:
6718 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3010 6351 7206 8877 9275 9549 9696 10107 3010
Size: 3341 855 1671 398 274 147 411 3347
Fragments arranged by size:
3347 3341 1671 855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3662 3692 5751 5781 6611
Size: 30 165 30 2259 30 2059 30 830
Cuts at: 6611 6641 6671 6701 1178
Size: 30 30 30 4921
Fragments arranged by size:
4921 2259 2059 830 165 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 3120 3382 4521 4604 4670 6031 6058
Size: 1126 808 262 1139 83 66 1361 27
Cuts at: 6058 6419 1186
Size: 361 5211
Fragments arranged by size:
5211 1361 1139 1126 808 361 262 83
66 27
BseYI C'CCAG_C
Cuts at: 2503 3854 4161 4759 4848 4957 5296 5380 5950
Size: 1351 307 598 89 109 339 84 570
Cuts at: 5950 6004 9647 10143 2503
Size: 54 3643 496 2804
Fragments arranged by size:
3643 2804 1351 598 570 496 339 307
109 89 84 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3448 3852 3959 4376 7117 7663 1163
Size: 573 1712 404 107 417 2741 546 3944
Fragments arranged by size:
3944 2741 1712 573 546 417 404 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7894 8655 9683 1540
Size: 275 186 5893 761 1028 2301
Fragments arranged by size:
5893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2347 3079 3611 3708 4134 4263 4842 5549 5630
Size: 732 532 97 426 129 579 707 81
Cuts at: 5630 5853 5916 6041 6753 7692 8505 8590 9661
Size: 223 63 125 712 939 813 85 1071
Cuts at: 9661 2347
Size: 3130
Fragments arranged by size:
3130 1071 939 813 732 712 707 579
532 426 223 129 125 97 85 81
63
BsiWI C'GTAC_G
Cuts at: 3613 3613
Size: 10444
BsmI GAATG_Cn'
Cuts at: 3239 3769 4415 5020 6850 7055 7955 8048 3239
Size: 530 646 605 1830 205 900 93 5635
Fragments arranged by size:
5635 1830 900 646 605 530 205 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4631 5473 4631
Size: 842 9602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2347 2483 2620 3079 3611 3633
Size: 399 400 157 136 137 459 532 22
Cuts at: 3633 3708 4079 4134 4263 4459 4842 5070 5549
Size: 75 371 55 129 196 383 228 479
Cuts at: 5549 5630 5853 5916 6041 6339 6753 6800 6915
Size: 81 223 63 125 298 414 47 115
Cuts at: 6915 7305 7692 7763 8505 8590 9661 1391
Size: 390 387 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 479 459 414 400
399 390 387 383 371 298 228 223
196 157 137 136 129 125 115 85
81 75 71 63 55 47 22
BspEI T'CCGG_A
Cuts at: 10107 10107
Size: 10444
BspHI T'CATG_A
Cuts at: 6540 8333 10063 6540
Size: 1793 1730 6921
Fragments arranged by size:
6921 1793 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2566 2641 3692 3758 3766 4094 4797 4843
Size: 1722 75 1051 66 8 328 703 46
Cuts at: 4843 4939 5689 6192 7652 7685 7799 844
Size: 96 750 503 1460 33 114 3489
Fragments arranged by size:
3489 1722 1460 1051 750 703 503 328
114 96 75 66 46 33 8
BsrBI CCG'CTC
Cuts at: 1394 1823 7345 8331 1394
Size: 429 5522 986 3507
Fragments arranged by size:
5522 3507 986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5056 5164 5715 7258 7296 8939 9113
Size: 1183 3808 108 551 1543 38 1643 174
Cuts at: 9113 65
Size: 1396
Fragments arranged by size:
3808 1643 1543 1396 1183 551 174 108
38
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 3692 4370 9088 9275 1700
Size: 110 752 1130 678 4718 187 2869
Fragments arranged by size:
4718 2869 1130 752 678 187 110
BsrGI T'GTAC_A
Cuts at: 96 2694 6091 96
Size: 2598 3397 4449
Fragments arranged by size:
4449 3397 2598
BssHII G'CGCG_C
Cuts at: 1925 5903 1925
Size: 3978 6466
BssSI C'ACGA_G
Cuts at: 8504 9516 8504
Size: 1012 9432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2892 5314 7140 794
Size: 2098 2422 1826 4098
Fragments arranged by size:
4098 2422 2098 1826
BstBI TT'CG_AA
Cuts at: 2160 9248 2160
Size: 7088 3356
BstEII G'GTnAC_C
Cuts at: 1184 2177 2578 2827 3796 4241 7329 1184
Size: 993 401 249 969 445 3088 4299
Fragments arranged by size:
4299 3088 993 969 445 401 249
BstXI CCAn_nnnn'nTGG
Cuts at: 2912 5026 6838 7180 2912
Size: 2114 1812 342 6176
Fragments arranged by size:
6176 2114 1812 342
BstYI r'GATC_y
Cuts at: 1972 2062 3990 4383 4755 5835 6111 6416 6708
Size: 90 1928 393 372 1080 276 305 292
Cuts at: 6708 7065 7774 8141 8526 8543 9995 10081 10093
Size: 357 709 367 385 17 1452 86 12
Cuts at: 10093 10439 1972
Size: 346 1977
Fragments arranged by size:
1977 1928 1452 1080 709 393 385 372
367 357 346 305 292 276 90 86
17 12
BstZ17I GTA'TAC
Cuts at: 2316 2316
Size: 10444
Bsu36I CC'TnA_GG
Cuts at: 2422 4550 5490 5736 2422
Size: 2128 940 246 7130
Fragments arranged by size:
7130 2128 940 246
BtgI C'CryG_G
Cuts at: 513 1065 2004 2665 3216 3736 4463 5079 5151
Size: 552 939 661 551 520 727 616 72
Cuts at: 5151 5562 7154 513
Size: 411 1592 3803
Fragments arranged by size:
3803 1592 939 727 661 616 552 551
520 411 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9705
Fragments arranged by size:
9705 683 56
BtsI GCAGTG_nn'
Cuts at: 2344 2454 2603 3465 3611 3864 5005 5031 5483
Size: 110 149 862 146 253 1141 26 452
Cuts at: 5483 5914 5930 6091 7145 7960 8754 8774 2344
Size: 431 16 161 1054 815 794 20 4014
Fragments arranged by size:
4014 1141 1054 862 815 794 452 431
253 161 149 146 110 26 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2320 2355 529
Size: 35 1756 35 8618
Fragments arranged by size:
8618 1756 35 35
DraI TTT'AAA
Cuts at: 7748 7748
Size: 10444
DraIII CAC_nnn'GTG
Cuts at: 6020 7140 6020
Size: 1120 9324
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9451 809
Size: 507 8135 1802
Fragments arranged by size:
8135 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2560 4300 4372
Size: 54 1425 50 275 18 730 1740 72
Cuts at: 4372 4562 4875 5533 5898 6828 7014 7891 8780
Size: 190 313 658 365 930 186 877 889
Cuts at: 8780 10409 8
Size: 1629 43
Fragments arranged by size:
1740 1629 1425 930 889 877 730 658
365 313 275 190 186 72 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7891 1537
Size: 275 6079 4090
Fragments arranged by size:
6079 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3105 3113 3809 3821 4619 4821 5637 6285
Size: 1752 8 696 12 798 202 816 648
Cuts at: 6285 7725 7734 8374 1353
Size: 1440 9 640 3423
Fragments arranged by size:
3423 1752 1440 816 798 696 648 640
202 12 9 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4655 5555 9031 9405 9551 387
Size: 1064 138 3066 900 3476 374 146 1280
Fragments arranged by size:
3476 3066 1280 1064 900 374 146 138
EcoICRI GAG'CTC
Cuts at: 3706 5628 6039 7690 3706
Size: 1922 411 1651 6460
Fragments arranged by size:
6460 1922 1651 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2427 2881 2888 2990 3002 3126 3354
Size: 393 795 454 7 102 12 124 228
Cuts at: 3354 3538 3584 3981 4128 4301 4643 4769 5046
Size: 184 46 397 147 173 342 126 277
Cuts at: 5046 5129 5165 5339 5559 6309 6638 6860 6878
Size: 83 36 174 220 750 329 222 18
Cuts at: 6878 6915 7124 7130 7389 8507 9104 9891 10159
Size: 37 209 6 259 1118 597 787 268
Cuts at: 10159 1239
Size: 1524
Fragments arranged by size:
1524 1118 795 787 750 597 454 397
393 342 329 277 268 259 228 222
220 209 184 174 173 147 126 124
102 83 46 37 36 18 12 7
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 3728 5444 2093
Size: 1635 1716 7093
Fragments arranged by size:
7093 1716 1635
EcoO109I rG'GnC_Cy
Cuts at: 2098 3091 3621 3886 4492 4545 5066 5067 5596
Size: 993 530 265 606 53 521 1 529
Cuts at: 5596 6181 6862 2098
Size: 585 681 5680
Fragments arranged by size:
5680 993 681 606 585 530 529 521
265 53 1
EcoRI G'AATT_C
Cuts at: 7753 8131 7753
Size: 378 10066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6883 7406 6883
Size: 523 9921
FseI GG_CCGG'CC
Cuts at: 2566 2566
Size: 10444
FspI TGC'GCA
Cuts at: 8950 8950
Size: 10444
HaeII r_GCGC'y
Cuts at: 1678 3792 4107 9591 1678
Size: 2114 315 5484 2531
Fragments arranged by size:
5484 2531 2114 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2534 3392 3424 4850
Size: 32 135 32 930 32 858 32 1426
Cuts at: 4850 4882 4934 4966 5594 5626 5779 5811 6611
Size: 32 52 32 628 32 153 32 800
Cuts at: 6611 6643 7052 7084 7526 7558 9085 9117 9159
Size: 32 409 32 442 32 1527 32 42
Cuts at: 9159 9191 1373
Size: 32 2626
Fragments arranged by size:
2626 1527 1426 930 858 800 628 442
409 153 135 52 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 2367 2674 5974 6787 7657
Size: 988 281 139 290 307 3300 813 870
Cuts at: 7657 7788 8034 669
Size: 131 246 3079
Fragments arranged by size:
3300 3079 988 870 813 307 290 281
246 139 131
HindIII A'AGCT_T
Cuts at: 748 7383 748
Size: 6635 3809
HpaI GTT'AAC
Cuts at: 8034 8034
Size: 10444
KpnI G_GTAC'C
Cuts at: 7769 7769
Size: 10444
MfeI C'AATT_G
Cuts at: 8043 8043
Size: 10444
MluI A'CGCG_T
Cuts at: 10320 10320
Size: 10444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3498 7095 9558 9742 1305
Size: 2193 3597 2463 184 2007
Fragments arranged by size:
3597 2463 2193 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4302 4564 4877 5535 6830
Size: 54 1425 343 2470 262 313 658 1295
Cuts at: 6830 7016 10
Size: 186 3438
Fragments arranged by size:
3438 2470 1425 1295 658 343 313 262
186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 5858 5984 6608 6932 8402 8761
Size: 648 2196 2496 126 624 324 1470 359
Cuts at: 8761 8920 518
Size: 159 2042
Fragments arranged by size:
2496 2196 2042 1470 648 624 359 324
159 126
MspA1I CmG'CkG
Cuts at: 1606 2229 2741 3462 4485 4687 4795 6008 7860
Size: 623 512 721 1023 202 108 1213 1852
Cuts at: 7860 8537 9685 9930 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1852 1213 1148 1023 721 677 623
512 245 202 108
NaeI GCC'GGC
Cuts at: 2564 3694 4372 2564
Size: 1130 678 8636
Fragments arranged by size:
8636 1130 678
NcoI C'CATG_G
Cuts at: 513 1065 2004 2665 3216 3736 5151 7154 513
Size: 552 939 661 551 520 1415 2003 3803
Fragments arranged by size:
3803 2003 1415 939 661 552 551 520
NdeI CA'TA_TG
Cuts at: 387 6073 387
Size: 5686 4758
NgoMIV G'CCGG_C
Cuts at: 2562 3692 4370 2562
Size: 1130 678 8636
Fragments arranged by size:
8636 1130 678
NheI G'CTAG_C
Cuts at: 1052 5189 1052
Size: 4137 6307
NotI GC'GGCC_GC
Cuts at: 7891 7891
Size: 10444
NruI TCG'CGA
Cuts at: 7449 7449
Size: 10444
NspI r_CATG'y
Cuts at: 1132 1229 2650 4370 6156 6703 6937 7802 9347
Size: 97 1421 1720 1786 547 234 865 1545
Cuts at: 9347 1132
Size: 2229
Fragments arranged by size:
2229 1786 1720 1545 1421 865 547 234
97
PasI CC'CwG_GG
Cuts at: 1348 2925 4204 1348
Size: 1577 1279 7588
Fragments arranged by size:
7588 1577 1279
PciI A'CATG_T
Cuts at: 6933 9343 6933
Size: 2410 8034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2761 5394 6577 1108
Size: 1653 2633 1183 4975
Fragments arranged by size:
4975 2633 1653 1183
PfoI T'CCnGG_A
Cuts at: 3317 3348 3714 4050 4062 5612 5838 6177 6615
Size: 31 366 336 12 1550 226 339 438
Cuts at: 6615 3317
Size: 7146
Fragments arranged by size:
7146 1550 438 366 339 336 226 31
12
PmeI GTTT'AAAC
Cuts at: 7748 7748
Size: 10444
PmlI CAC'GTG
Cuts at: 2700 2700
Size: 10444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 8513 8545 10026 10058 4316
Size: 32 4165 32 1481 32 4702
Fragments arranged by size:
4702 4165 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3091 3621 4492 4545 6181 6862 3091
Size: 530 871 53 1636 681 6673
Fragments arranged by size:
6673 1636 871 681 530 53
PshAI GACnn'nnGTC
Cuts at: 1613 3011 6822 1613
Size: 1398 3811 5235
Fragments arranged by size:
5235 3811 1398
PsiI TTA'TAA
Cuts at: 8014 8014
Size: 10444
PspOMI G'GGCC_C
Cuts at: 5066 5066
Size: 10444
PstI C_TGCA'G
Cuts at: 830 2610 2878 3048 3606 3788 3885 4794 7796
Size: 1780 268 170 558 182 97 909 3002
Cuts at: 7796 830
Size: 3478
Fragments arranged by size:
3478 3002 1780 909 558 268 182 170
97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10444
PvuII CAG'CTG
Cuts at: 2741 3462 4485 4687 6008 2741
Size: 721 1023 202 1321 7177
Fragments arranged by size:
7177 1321 1023 721 202
SacI G_AGCT'C
Cuts at: 3708 5630 6041 7692 3708
Size: 1922 411 1651 6460
Fragments arranged by size:
6460 1922 1651 411
SalI G'TCGA_C
Cuts at: 1936 7786 1936
Size: 5850 4594
SanDI GG'GwC_CC
Cuts at: 3621 4545 3621
Size: 924 9520
SapI GCTCTTCn'nnn_
Cuts at: 6285 6285
Size: 10444
SbfI CC_TGCA'GG
Cuts at: 3048 3885 7796 3048
Size: 837 3911 5696
Fragments arranged by size:
5696 3911 837
ScaI AGT'ACT
Cuts at: 8692 10404 8692
Size: 1712 8732
SfcI C'TryA_G
Cuts at: 826 1045 2606 2874 3044 3496 3602 3784 3881
Size: 219 1561 268 170 452 106 182 97
Cuts at: 3881 4436 4790 6100 7792 8927 9608 9799 826
Size: 555 354 1310 1692 1135 681 191 1471
Fragments arranged by size:
1692 1561 1471 1310 1135 681 555 452
354 268 219 191 182 170 106 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 4561 4561
Size: 10444
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10444
SmaI CCC'GGG
Cuts at: 1872 7771 1872
Size: 5899 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2068 2588 4391 5204 5417 6761
Size: 197 926 125 520 1803 813 213 1344
Cuts at: 6761 7391 8548 9449 9711 820
Size: 630 1157 901 262 1553
Fragments arranged by size:
1803 1553 1344 1157 926 901 813 630
520 262 213 197 125
SnaBI TAC'GTA
Cuts at: 493 8235 493
Size: 7742 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10444
SphI G_CATG'C
Cuts at: 1132 2650 4370 7802 1132
Size: 1518 1720 3432 3774
Fragments arranged by size:
3774 3432 1720 1518
SspI AAT'ATT
Cuts at: 5 52 8368 5
Size: 47 8316 2081
Fragments arranged by size:
8316 2081 47
StuI AGG'CCT
Cuts at: 1517 2729 3084 4094 4555 1517
Size: 1212 355 1010 461 7406
Fragments arranged by size:
7406 1212 1010 461 355
StyI C'CwwG_G
Cuts at: 513 1065 2004 2665 2755 2954 3216 3481 3736
Size: 552 939 661 90 199 262 265 255
Cuts at: 3736 3768 5019 5151 5592 7011 7080 7154 7200
Size: 32 1251 132 441 1419 69 74 46
Cuts at: 7200 7883 513
Size: 683 3074
Fragments arranged by size:
3074 1419 1251 939 683 661 552 441
265 262 255 199 132 90 74 69
46 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3886 4136 4175 4949 8471 8488 8641
Size: 1255 2306 250 39 774 3522 17 153
Cuts at: 8641 8826 9271 325
Size: 185 445 1498
Fragments arranged by size:
3522 2306 1498 1255 774 445 250 185
153 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 4721 6091
Size: 274 80 33 51 1443 717 2027 1370
Cuts at: 6091 6696 6726 7218 7638 8690 10402 96
Size: 605 30 492 420 1052 1712 138
Fragments arranged by size:
2027 1712 1443 1370 1052 717 605 492
420 274 138 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3861 3893 5751 5783 6671 6703 10404 10436 3861
Size: 32 1858 32 888 32 3701 32 3869
Fragments arranged by size:
3869 3701 1858 888 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4816 6052 1316
Size: 3500 1236 5708
Fragments arranged by size:
5708 3500 1236
XbaI T'CTAG_A
Cuts at: 7780 7780
Size: 10444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 6607 2758
Size: 3849 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10444
XmaI C'CCGG_G
Cuts at: 1870 7769 1870
Size: 5899 4545
XmnI GAAnn'nnTTC
Cuts at: 6485 8573 6485
Size: 2088 8356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8252 276
Size: 53 83 186 936 6718 2468
Fragments arranged by size:
6718 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI EcoRV FspAI KasI
NarI NsiI PacI PspXI PsrI RsrII SacII SexAI
SfoI SrfI SwaI