Restriction map for
pFN21AB1440
(Circular) (Six-base) MAPSORT of: pFN21AB1440.seq Check: 5381 from: 1 to: 10399
pFN21AB1440 10399 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7429 7429
Size: 10399
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4299 5057 8209 278
Size: 53 83 186 936 2763 758 3152 2468
Fragments arranged by size:
3152 2763 2468 936 758 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 3351 4185 4766 7572 7742 1937
Size: 592 822 834 581 2806 170 4594
Fragments arranged by size:
4594 2806 834 822 592 581 170
Acc65I G'GTAC_C
Cuts at: 5386 7720 5386
Size: 2334 8065
AclI AA'CG_TT
Cuts at: 1563 8526 8899 1563
Size: 6963 373 3063
Fragments arranged by size:
6963 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 3740 5546 5903 8462 9846 10114 1632
Size: 821 1287 1806 357 2559 1384 268 1917
Fragments arranged by size:
2559 1917 1806 1384 1287 821 357 268
AfeI AGC'GCT
Cuts at: 2234 5684 7247 2234
Size: 3450 1563 5386
Fragments arranged by size:
5386 3450 1563
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10202
AflIII A'CryG_T
Cuts at: 1203 2648 6768 6933 7575 9298 10275 1203
Size: 1445 4120 165 642 1723 977 1327
Fragments arranged by size:
4120 1723 1445 1327 977 642 165
AgeI A'CCGG_T
Cuts at: 9230 9230
Size: 10399
AhdI GACnn_n'nnGTC
Cuts at: 9128 9128
Size: 10399
AleI CACnn'nnGTG
Cuts at: 1166 2897 3425 3482 4070 4175 4247 4835 6468
Size: 1731 528 57 588 105 72 588 1633
Cuts at: 6468 6818 7494 7517 1166
Size: 350 676 23 4048
Fragments arranged by size:
4048 1731 1633 676 588 588 528 350
105 72 57 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3455 4115 5034 7512 1184
Size: 2271 660 919 2478 4071
Fragments arranged by size:
4071 2478 2271 919 660
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6887 6919 6887
Size: 32 10367
AlwNI CAG_nnn'CTG
Cuts at: 2495 2663 4867 5245 6856 8142 9714 2495
Size: 168 2204 378 1611 1286 1572 3180
Fragments arranged by size:
3180 2204 1611 1572 1286 378 168
ApaI G_GGCC'C
Cuts at: 3089 4079 6254 6606 3089
Size: 990 2175 352 6882
Fragments arranged by size:
6882 2175 990 352
ApaLI G'TGCA_C
Cuts at: 2775 6614 7487 8456 9612 2775
Size: 3839 873 969 1156 3562
Fragments arranged by size:
3839 3562 1156 969 873
ApoI r'AATT_y
Cuts at: 1493 6082 7708 7934 8086 10195 1493
Size: 4589 1626 226 152 2109 1697
Fragments arranged by size:
4589 2109 1697 1626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8953 160
Size: 872 7921 1606
Fragments arranged by size:
7921 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10399
AvaI C'yCGr_G
Cuts at: 1870 1943 3314 4085 4709 5438 6147 6179 6260
Size: 73 1371 771 624 729 709 32 81
Cuts at: 6260 6345 6899 7338 7724 1870
Size: 85 554 439 386 4545
Fragments arranged by size:
4545 1371 771 729 709 624 554 439
386 85 81 73 32
BamHI G'GATC_C
Cuts at: 2280 3585 7729 8096 2280
Size: 1305 4144 367 4583
Fragments arranged by size:
4583 4144 1305 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2081 2573 3238 4394
Size: 325 217 627 251 43 492 665 1156
Cuts at: 4394 4497 5386 6380 6820 7720 9175 618
Size: 103 889 994 440 900 1455 1842
Fragments arranged by size:
1842 1455 1156 994 900 889 665 627
492 440 325 251 217 103 43
BanII G_rGCy'C
Cuts at: 1391 2820 2865 3089 3324 3417 3863 4079 4272
Size: 1429 45 224 235 93 446 216 193
Cuts at: 4272 6254 6606 6900 7052 7718 1391
Size: 1982 352 294 152 666 4072
Fragments arranged by size:
4072 1982 1429 666 446 352 294 235
224 216 193 152 93 45
BbeI G_GCGC'C
Cuts at: 2085 3242 2085
Size: 1157 9242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 3669 3772 5057 6698 7598 928
Size: 971 199 1571 103 1285 1641 900 3729
Fragments arranged by size:
3729 1641 1571 1285 971 900 199 103
BbvCI CC'TCA_GC
Cuts at: 2427 3140 2427
Size: 713 9686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8590 8624 1903
Size: 34 6653 34 3678
Fragments arranged by size:
6653 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 8291 9501 3335
Size: 4956 1210 4233
Fragments arranged by size:
4956 4233 1210
BclI T'GATC_A
Cuts at: 1548 4458 6358 1548
Size: 2910 1900 5589
Fragments arranged by size:
5589 2910 1900
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3912 3944 4132
Size: 107 122 71 631 605 2240 32 188
Cuts at: 4132 4562 6642 9010 136
Size: 430 2080 2368 1525
Fragments arranged by size:
2368 2240 2080 1525 631 605 430 188
122 107 71 32
BglII A'GATC_T
Cuts at: 10394 10394
Size: 10399
BlpI GC'TnA_GC
Cuts at: 2864 3278 5066 7816 2864
Size: 414 1788 2750 5447
Fragments arranged by size:
5447 2750 1788 414
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2779 3080 3089 3223 4063 4079 4128
Size: 253 736 301 9 134 840 16 49
Cuts at: 4128 4399 5106 5151 6254 6606 6618 7404 7491
Size: 271 707 45 1103 352 12 786 87
Cuts at: 7491 8460 9616 1790
Size: 969 1156 2573
Fragments arranged by size:
2573 1156 1103 969 840 786 736 707
352 301 271 253 134 87 49 45
16 12 9
BmgBI CAC'GTC
Cuts at: 1318 4072 4123 4764 6936 1318
Size: 2754 51 641 2172 4781
Fragments arranged by size:
4781 2754 2172 641 51
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2142 3655 3805
Size: 416 53 83 399 24 725 1513 150
Cuts at: 3805 3919 7297 8130 9088 442
Size: 114 3378 833 958 1753
Fragments arranged by size:
3378 1753 1513 958 833 725 416 399
150 114 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4307 1056
Size: 3251 7148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2879 3229 3988 4046 4277 4475 5468 5677
Size: 1640 350 759 58 231 198 993 209
Cuts at: 5677 6113 6301 6353 6460 6812 6855 7565 7685
Size: 436 188 52 107 352 43 710 120
Cuts at: 7685 9059 1239
Size: 1374 2579
Fragments arranged by size:
2579 1640 1374 993 759 710 436 352
350 231 209 198 188 120 107 58
52 43
Bpu10I CC'TnA_GC
Cuts at: 2013 2427 3140 2013
Size: 414 713 9272
Fragments arranged by size:
9272 713 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4895 5650 6011 8524 9389 9687 4895
Size: 755 361 2513 865 298 5607
Fragments arranged by size:
5607 2513 865 755 361 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 5952 6257 6380 9062 882
Size: 617 2841 1612 305 123 2682 2219
Fragments arranged by size:
2841 2682 2219 1612 617 305 123
BsaAI yAC'GTr
Cuts at: 493 1204 2791 4249 6075 6715 6769 6804 8190
Size: 711 1587 1458 1826 640 54 35 1386
Cuts at: 8190 493
Size: 2702
Fragments arranged by size:
2702 1826 1587 1458 1386 711 640 54
35
BsaBI GATnn'nnATC
Cuts at: 10393 10393
Size: 10399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 3239 3777 4296
Size: 53 83 186 936 549 1157 538 519
Cuts at: 4296 5054 5420 6174 8206 8588 9223 275
Size: 758 366 754 2032 382 635 1451
Fragments arranged by size:
2032 1451 1157 936 758 754 635 549
538 519 382 366 186 83 53
BsaWI w'CCGG_w
Cuts at: 3361 5623 8832 9230 9504 9651 10062 3361
Size: 2262 3209 398 274 147 411 3698
Fragments arranged by size:
3698 3209 2262 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3680 3710 5000 5030 5747
Size: 30 165 30 2277 30 1290 30 717
Cuts at: 5747 5777 5833 5863 6032 6062 6326 6356 6749
Size: 30 56 30 169 30 264 30 393
Cuts at: 6749 6779 6887 6917 1178
Size: 30 108 30 4660
Fragments arranged by size:
4660 2277 1290 717 393 264 169 165
108 56 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2688 2828 2899 3332 3407 3688 4697 5504
Size: 1502 140 71 433 75 281 1009 807
Cuts at: 5504 5762 5896 6350 1186
Size: 258 134 454 5235
Fragments arranged by size:
5235 1502 1009 807 454 433 281 258
140 134 75 71
BseYI C'CCAG_C
Cuts at: 2229 2717 2914 3203 3500 3543 4008 4127 4205
Size: 488 197 289 297 43 465 119 78
Cuts at: 4205 4528 4721 4979 5070 5736 6856 9602 10098
Size: 323 193 258 91 666 1120 2746 496
Cuts at: 10098 2229
Size: 2530
Fragments arranged by size:
2746 2530 1120 666 496 488 465 323
297 289 258 197 193 119 91 78
43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2037 2318 3284 3320 3738 4452 4607
Size: 573 301 281 966 36 418 714 155
Cuts at: 4607 6770 7643 1163
Size: 2163 873 3919
Fragments arranged by size:
3919 2163 966 873 714 573 418 301
281 155 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2551 3426 5320 5877 7849 8610
Size: 275 186 550 875 1894 557 1972 761
Cuts at: 8610 9638 1540
Size: 1028 2301
Fragments arranged by size:
2301 1972 1894 1028 875 761 557 550
275 186
BsiHKAI G_wGCw'C
Cuts at: 2379 2779 2820 2865 4002 5435 5559 6618 6900
Size: 400 41 45 1137 1433 124 1059 282
Cuts at: 6900 7136 7167 7491 8460 8545 9616 2379
Size: 236 31 324 969 85 1071 3162
Fragments arranged by size:
3162 1433 1137 1071 1059 969 400 324
282 236 124 85 45 41 31
BsiWI C'GTAC_G
Cuts at: 2875 2875
Size: 10399
BsmI GAATG_Cn'
Cuts at: 6235 7910 8003 6235
Size: 1675 93 8631
Fragments arranged by size:
8631 1675 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2379 2779 2820 2865 3080 3089
Size: 399 253 336 400 41 45 215 9
Cuts at: 3089 3223 3324 3417 3863 4002 4063 4079 4128
Size: 134 101 93 446 139 61 16 49
Cuts at: 4128 4272 4399 5106 5151 5435 5559 6254 6606
Size: 144 127 707 45 284 124 695 352
Cuts at: 6606 6618 6900 7052 7136 7167 7404 7491 7718
Size: 12 282 152 84 31 237 87 227
Cuts at: 7718 8460 8545 9616 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 695 446 400 399
352 336 284 282 253 237 227 215
152 144 139 134 127 124 101 93
87 85 84 61 49 45 45 41
31 16 12 9
BspEI T'CCGG_A
Cuts at: 3361 5623 10062 3361
Size: 2262 4439 3698
Fragments arranged by size:
4439 3698 2262
BspHI T'CATG_A
Cuts at: 6318 8288 10018 6318
Size: 1970 1730 6699
Fragments arranged by size:
6699 1970 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4725 4749 5184 6728 6966 7429 7754 844
Size: 3881 24 435 1544 238 463 325 3489
Fragments arranged by size:
3881 3489 1544 463 435 325 238 24
BsrBI CCG'CTC
Cuts at: 1394 1823 3619 6676 8286 1394
Size: 429 1796 3057 1610 3507
Fragments arranged by size:
3507 3057 1796 1610 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2288 2875 5052 8894 9068 65
Size: 1183 1040 587 2177 3842 174 1396
Fragments arranged by size:
3842 2177 1396 1183 1040 587 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4443 4536 4616 5002 5610 6641 6981
Size: 110 2633 93 80 386 608 1031 340
Cuts at: 6981 9043 9230 1700
Size: 2062 187 2869
Fragments arranged by size:
2869 2633 2062 1031 608 386 340 187
110 93 80
BsrGI T'GTAC_A
Cuts at: 96 2324 5118 96
Size: 2228 2794 5377
Fragments arranged by size:
5377 2794 2228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10399
BssSI C'ACGA_G
Cuts at: 2539 3595 6686 8459 9471 2539
Size: 1056 3091 1773 1012 3467
Fragments arranged by size:
3467 3091 1773 1056 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2774 3535 5155 6645 7399 794
Size: 1980 761 1620 1490 754 3794
Fragments arranged by size:
3794 1980 1620 1490 761 754
BstBI TT'CG_AA
Cuts at: 9203 9203
Size: 10399
BstEII G'GTnAC_C
Cuts at: 1184 3679 4240 6406 1184
Size: 2495 561 2166 5177
Fragments arranged by size:
5177 2495 2166 561
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 3331 4777 5005 6470 7075 2419
Size: 912 1446 228 1465 605 5743
Fragments arranged by size:
5743 1465 1446 912 605 228
BstYI r'GATC_y
Cuts at: 1972 2280 3585 4224 7729 8096 8481 8498 9950
Size: 308 1305 639 3505 367 385 17 1452
Cuts at: 9950 10036 10048 10394 1972
Size: 86 12 346 1977
Fragments arranged by size:
3505 1977 1452 1305 639 385 367 346
308 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2121 3612 2121
Size: 1491 8908
BtgI C'CryG_G
Cuts at: 513 1065 2004 2086 3426 3483 3549 3867 3933
Size: 552 939 82 1340 57 66 318 66
Cuts at: 3933 3964 4139 4228 4273 4467 4833 5215 5670
Size: 31 175 89 45 194 366 382 455
Cuts at: 5670 6267 6376 6526 6606 6891 7036 7617 513
Size: 597 109 150 80 285 145 581 3295
Fragments arranged by size:
3295 1340 939 597 581 552 455 382
366 318 285 194 175 150 145 109
89 82 80 66 66 57 45 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3504 5797 6115 6223 487
Size: 683 56 2278 2293 318 108 4663
Fragments arranged by size:
4663 2293 2278 683 318 108 56
BtsI GCAGTG_nn'
Cuts at: 2599 2775 3219 3308 4170 4479 6616 7489 7501
Size: 176 444 89 862 309 2137 873 12
Cuts at: 7501 7915 8709 8729 2599
Size: 414 794 20 4269
Fragments arranged by size:
4269 2137 873 862 794 444 414 309
176 89 20 12
ClaI AT'CG_AT
Cuts at: 1554 6168 6210 1554
Size: 4614 42 5743
Fragments arranged by size:
5743 4614 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10364
DraI TTT'AAA
Cuts at: 7703 7703
Size: 10399
DraIII CAC_nnn'GTG
Cuts at: 3679 5248 3679
Size: 1569 8830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2137 4937 9406 809
Size: 507 821 2800 4469 1802
Fragments arranged by size:
4469 2800 1802 821 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2548 2845 2901
Size: 54 1425 50 275 18 718 297 56
Cuts at: 2901 3111 4219 4470 5317 6306 6368 6432 6518
Size: 210 1108 251 847 989 62 64 86
Cuts at: 6518 6709 6808 6915 7235 7385 7846 8735 10364
Size: 191 99 107 320 150 461 889 1629
Cuts at: 10364 8
Size: 43
Fragments arranged by size:
1629 1425 1108 989 889 847 718 461
320 297 275 251 210 191 150 107
99 86 64 62 56 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2548 5317 7846 1537
Size: 275 736 2769 2529 4090
Fragments arranged by size:
4090 2769 2529 736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 2961 8329 1353
Size: 1023 585 5368 3423
Fragments arranged by size:
5368 3423 1023 585
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2496 3814 4817 5499 6005 8986
Size: 1064 138 907 1318 1003 682 506 2981
Cuts at: 8986 9360 9506 387
Size: 374 146 1280
Fragments arranged by size:
2981 1318 1280 1064 1003 907 682 506
374 146 138
EcoICRI GAG'CTC
Cuts at: 2818 2863 6898 2818
Size: 45 4035 6319
Fragments arranged by size:
6319 4035 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2879 3229 3740 3988 4046 4277
Size: 393 821 426 350 511 248 58 231
Cuts at: 4277 4475 5468 5546 5677 5903 6113 6301 6353
Size: 198 993 78 131 226 210 188 52
Cuts at: 6353 6460 6812 6855 7565 7685 8462 9059 9846
Size: 107 352 43 710 120 777 597 787
Cuts at: 9846 10114 1239
Size: 268 1524
Fragments arranged by size:
1524 993 821 787 777 710 597 511
426 393 352 350 268 248 231 226
210 198 188 131 120 107 78 58
52 43
EcoNI CCTnn'n_nnAGG
Cuts at: 4625 6401 4625
Size: 1776 8623
EcoO109I rG'GnC_Cy
Cuts at: 2423 3085 3086 3715 3886 4012 4076 4704 4714
Size: 662 1 629 171 126 64 628 10
Cuts at: 4714 4786 5233 5774 5841 6250 6251 6602 7118
Size: 72 447 541 67 409 1 351 516
Cuts at: 7118 2423
Size: 5704
Fragments arranged by size:
5704 662 629 628 541 516 447 409
351 171 126 72 67 64 10 1
1
EcoRI G'AATT_C
Cuts at: 6082 7708 8086 6082
Size: 1626 378 8395
Fragments arranged by size:
8395 1626 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3700 5908 3700
Size: 2208 8191
FseI GG_CCGG'CC
Cuts at: 4447 4447
Size: 10399
FspI TGC'GCA
Cuts at: 4109 6449 8905 4109
Size: 2340 2456 5603
Fragments arranged by size:
5603 2456 2340
FspAI rTGC'GCAy
Cuts at: 4109 6449 4109
Size: 2340 8059
HaeII r_GCGC'y
Cuts at: 1678 2085 2236 3242 3285 5686 6007 6788 6954
Size: 407 151 1006 43 2401 321 781 166
Cuts at: 6954 7249 9546 1678
Size: 295 2297 2531
Fragments arranged by size:
2531 2401 2297 1006 781 407 321 295
166 151 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3333 3365 3557 3589 3680
Size: 32 135 32 1761 32 192 32 91
Cuts at: 3680 3712 4269 4301 5135 5167 5833 5855 5865
Size: 32 557 32 834 32 666 22 10
Cuts at: 5865 5887 6032 6064 6326 6358 7334 7366 9040
Size: 22 145 32 262 32 976 32 1674
Cuts at: 9040 9072 9114 9146 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1761 1674 976 834 666 557 262
192 145 135 91 42 32 32 32
32 32 32 32 32 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 7276 7573 7743 7989 669
Size: 988 281 5338 297 170 246 3079
Fragments arranged by size:
5338 3079 988 297 281 246 170
HindIII A'AGCT_T
Cuts at: 748 7196 748
Size: 6448 3951
HpaI GTT'AAC
Cuts at: 7989 7989
Size: 10399
KasI G'GCGC_C
Cuts at: 2081 3238 2081
Size: 1157 9242
KpnI G_GTAC'C
Cuts at: 5390 7724 5390
Size: 2334 8065
MfeI C'AATT_G
Cuts at: 7998 7998
Size: 10399
MluI A'CGCG_T
Cuts at: 10275 10275
Size: 10399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2628 3555 6396 6651 9513 9697 1305
Size: 1323 927 2841 255 2862 184 2007
Fragments arranged by size:
2862 2841 2007 1323 927 255 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 3113 4221 6370 6434
Size: 54 1425 343 1071 210 1108 2149 64
Cuts at: 6434 6520 6711 6917 7237 7387 10
Size: 86 191 206 320 150 3022
Fragments arranged by size:
3022 2149 1425 1108 1071 343 320 210
206 191 150 86 64 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2417 2897 3296 3425 3482 4070
Size: 648 1035 216 480 399 129 57 588
Cuts at: 4070 4175 4247 4454 4472 4835 5003 5381 6468
Size: 105 72 207 18 363 168 378 1087
Cuts at: 6468 6611 6818 7184 7494 7517 8357 8716 8875
Size: 143 207 366 310 23 840 359 159
Cuts at: 8875 518
Size: 2042
Fragments arranged by size:
2042 1087 1035 840 648 588 480 399
378 366 363 359 310 216 207 207
168 159 143 129 105 72 57 23
18
MspA1I CmG'CkG
Cuts at: 1606 2392 2431 2563 3074 3551 3748 3966 4008
Size: 786 39 132 511 477 197 218 42
Cuts at: 4008 4131 4141 4230 4332 4725 5070 5099 5290
Size: 123 10 89 102 393 345 29 191
Cuts at: 5290 5497 7645 7815 8492 9640 9885 1606
Size: 207 2148 170 677 1148 245 2120
Fragments arranged by size:
2148 2120 1148 786 677 511 477 393
345 245 218 207 197 191 170 132
123 102 89 42 39 29 10
NaeI GCC'GGC
Cuts at: 4445 4445
Size: 10399
NarI GG'CG_CC
Cuts at: 2082 3239 2082
Size: 1157 9242
NcoI C'CATG_G
Cuts at: 513 1065 2004 2086 5670 6267 6526 6606 6891
Size: 552 939 82 3584 597 259 80 285
Cuts at: 6891 7617 513
Size: 726 3295
Fragments arranged by size:
3584 3295 939 726 597 552 285 259
82 80
NdeI CA'TA_TG
Cuts at: 387 5533 387
Size: 5146 5253
NgoMIV G'CCGG_C
Cuts at: 4443 4443
Size: 10399
NheI G'CTAG_C
Cuts at: 1052 4303 1052
Size: 3251 7148
NotI GC'GGCC_GC
Cuts at: 7846 7846
Size: 10399
NspI r_CATG'y
Cuts at: 1132 1229 2763 3277 3977 4184 4748 5416 6727
Size: 97 1534 514 700 207 564 668 1311
Cuts at: 6727 7156 7300 7555 7579 7757 9302 1132
Size: 429 144 255 24 178 1545 2229
Fragments arranged by size:
2229 1545 1534 1311 700 668 564 514
429 255 207 178 144 97 24
PasI CC'CwG_GG
Cuts at: 1348 2024 2399 1348
Size: 676 375 9348
Fragments arranged by size:
9348 676 375
PciI A'CATG_T
Cuts at: 7575 9298 7575
Size: 1723 8676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6377 6527 6856 1108
Size: 5269 150 329 4651
Fragments arranged by size:
5269 4651 329 150
PfoI T'CCnGG_A
Cuts at: 2856 4991 5559 7074 7542 2856
Size: 2135 568 1515 468 5713
Fragments arranged by size:
5713 2135 1515 568 468
PmeI GTTT'AAAC
Cuts at: 7703 7703
Size: 10399
PmlI CAC'GTG
Cuts at: 6075 6715 6804 6075
Size: 640 89 9670
Fragments arranged by size:
9670 640 89
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6887 6919 8468 8500 9981 10013 6887
Size: 32 1549 32 1481 32 7273
Fragments arranged by size:
7273 1549 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3715 3886 4012 4704 4714 4786 5774 5841 3715
Size: 171 126 692 10 72 988 67 8273
Fragments arranged by size:
8273 988 692 171 126 72 67 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10399
PsiI TTA'TAA
Cuts at: 7969 7969
Size: 10399
PspOMI G'GGCC_C
Cuts at: 3085 4075 6250 6602 3085
Size: 990 2175 352 6882
Fragments arranged by size:
6882 2175 990 352
PspXI vC'TCGA_Gb
Cuts at: 6899 6899
Size: 10399
PstI C_TGCA'G
Cuts at: 830 4545 4872 5181 5241 6623 7029 7496 7751
Size: 3715 327 309 60 1382 406 467 255
Cuts at: 7751 830
Size: 3478
Fragments arranged by size:
3715 3478 1382 467 406 327 309 255
60
PvuI CG_AT'CG
Cuts at: 2001 5877 2001
Size: 3876 6523
PvuII CAG'CTG
Cuts at: 2392 2431 2563 3748 4008 5070 5099 7645 2392
Size: 39 132 1185 260 1062 29 2546 5146
Fragments arranged by size:
5146 2546 1185 1062 260 132 39 29
RsrII CG'GwC_CG
Cuts at: 3871 3871
Size: 10399
SacI G_AGCT'C
Cuts at: 2820 2865 6900 2820
Size: 45 4035 6319
Fragments arranged by size:
6319 4035 45
SacII CC_GC'GG
Cuts at: 3552 3967 4142 4231 3552
Size: 415 175 89 9720
Fragments arranged by size:
9720 415 175 89
SalI G'TCGA_C
Cuts at: 1936 7571 7741 1936
Size: 5635 170 4594
Fragments arranged by size:
5635 4594 170
SanDI GG'GwC_CC
Cuts at: 3886 4786 5774 3886
Size: 900 988 8511
Fragments arranged by size:
8511 988 900
SapI GCTCTTCn'nnn_
Cuts at: 2961 2961
Size: 10399
SbfI CC_TGCA'GG
Cuts at: 7751 7751
Size: 10399
ScaI AGT'ACT
Cuts at: 3929 4547 5195 6365 7232 7265 8647 10359 3929
Size: 618 648 1170 867 33 1382 1712 3969
Fragments arranged by size:
3969 1712 1382 1170 867 648 618 33
SexAI A'CCwGG_T
Cuts at: 4014 4236 5244 4014
Size: 222 1008 9169
Fragments arranged by size:
9169 1008 222
SfcI C'TryA_G
Cuts at: 826 1045 2172 2526 3124 3454 4541 4868 5177
Size: 219 1127 354 598 330 1087 327 309
Cuts at: 5177 5237 6619 7025 7492 7747 8882 9563 9754
Size: 60 1382 406 467 255 1135 681 191
Cuts at: 9754 826
Size: 1471
Fragments arranged by size:
1471 1382 1135 1127 1087 681 598 467
406 354 330 327 309 255 219 191
60
SfoI GGC'GCC
Cuts at: 2083 3240 2083
Size: 1157 9242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10399
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10399
SmaI CCC'GGG
Cuts at: 1872 6181 6347 7340 7726 1872
Size: 4309 166 993 386 4545
Fragments arranged by size:
4545 4309 993 386 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4910 5629 6026 6147 6899 8503
Size: 197 926 2967 719 397 121 752 1604
Cuts at: 8503 9404 9666 820
Size: 901 262 1553
Fragments arranged by size:
2967 1604 1553 926 901 752 719 397
262 197 121
SnaBI TAC'GTA
Cuts at: 493 8190 493
Size: 7697 2702
SpeI A'CTAG_T
Cuts at: 152 2357 152
Size: 2205 8194
SphI G_CATG'C
Cuts at: 1132 3277 5416 6727 7156 7555 7757 1132
Size: 2145 2139 1311 429 399 202 3774
Fragments arranged by size:
3774 2145 2139 1311 429 399 202
SspI AAT'ATT
Cuts at: 5 52 8323 5
Size: 47 8271 2081
Fragments arranged by size:
8271 2081 47
StuI AGG'CCT
Cuts at: 1517 6581 7100 1517
Size: 5064 519 4816
Fragments arranged by size:
5064 4816 519
StyI C'CwwG_G
Cuts at: 513 1065 2004 2086 2608 2823 4383 4506 5423
Size: 552 939 82 522 215 1560 123 917
Cuts at: 5423 5574 5670 6267 6526 6606 6891 6924 7452
Size: 151 96 597 259 80 285 33 528
Cuts at: 7452 7617 7838 513
Size: 165 221 3074
Fragments arranged by size:
3074 1560 939 917 597 552 528 522
285 259 221 215 165 151 123 96
82 80 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 2762 2794 3938 4042 4515 4519
Size: 1255 753 429 32 1144 104 473 4
Cuts at: 4519 4667 5755 5979 5983 8426 8443 8596 8781
Size: 148 1088 224 4 2443 17 153 185
Cuts at: 8781 9226 325
Size: 445 1498
Fragments arranged by size:
2443 1498 1255 1144 1088 753 473 445
429 224 185 153 148 104 32 17
4 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2324 3642 3927
Size: 274 80 33 51 1443 347 1318 285
Cuts at: 3927 4545 5118 5193 6363 7230 7263 7290 8645
Size: 618 573 75 1170 867 33 27 1355
Cuts at: 8645 10357 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1355 1318 1170 867 618 573
347 285 274 138 80 75 51 33
33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5234 5266 5747 5779 5837 5869 10359 10391 5234
Size: 32 481 32 58 32 4490 32 5242
Fragments arranged by size:
5242 4490 481 58 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4762 1316
Size: 3446 6953
XbaI T'CTAG_A
Cuts at: 5464 7735 5464
Size: 2271 8128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 3292 4012 5398 5983 7039 2911
Size: 381 720 1386 585 1056 6271
Fragments arranged by size:
6271 1386 1056 720 585 381
XhoI C'TCGA_G
Cuts at: 1943 6147 6899 1943
Size: 4204 752 5443
Fragments arranged by size:
5443 4204 752
XmaI C'CCGG_G
Cuts at: 1870 6179 6345 7338 7724 1870
Size: 4309 166 993 386 4545
Fragments arranged by size:
4545 4309 993 386 166
XmnI GAAnn'nnTTC
Cuts at: 8528 8528
Size: 10399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4297 5055 8207 276
Size: 53 83 186 936 2763 758 3152 2468
Fragments arranged by size:
3152 2763 2468 936 758 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I EcoRV
NruI NsiI PacI PsrI SfiI SrfI SwaI