Restriction map for
pFN21AB1393
(Circular) (Six-base) MAPSORT of: pFN21AB1393.seq Check: 2628 from: 1 to: 7135
pFN21AB1393 7135 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 2134
Size: 7135
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4945 278
Size: 53 83 186 936 3409 2468
Fragments arranged by size:
3409 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4359 4478 1937
Size: 2422 119 4594
Fragments arranged by size:
4594 2422 119
Acc65I G'GTAC_C
Cuts at: 4456 4456
Size: 7135
AclI AA'CG_TT
Cuts at: 1563 5262 5635 1563
Size: 3699 373 3063
Fragments arranged by size:
3699 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2262 2331 2345 2508 2841 3927 5198
Size: 434 196 69 14 163 333 1086 1271
Cuts at: 5198 6582 6850 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1271 1086 434 333 268 196
163 69 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6938
AflIII A'CryG_T
Cuts at: 1203 6034 7011 1203
Size: 4831 977 1327
Fragments arranged by size:
4831 1327 977
AgeI A'CCGG_T
Cuts at: 5966 5966
Size: 7135
AhdI GACnn_n'nnGTC
Cuts at: 5864 5864
Size: 7135
AleI CACnn'nnGTG
Cuts at: 1166 2070 3212 1166
Size: 904 1142 5089
Fragments arranged by size:
5089 1142 904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7135
AlwNI CAG_nnn'CTG
Cuts at: 2263 4878 6450 2263
Size: 2615 1572 2948
Fragments arranged by size:
2948 2615 1572
ApaLI G'TGCA_C
Cuts at: 5192 6348 5192
Size: 1156 5979
ApoI r'AATT_y
Cuts at: 1493 2607 3346 4444 4670 4822 6931 1493
Size: 1114 739 1098 226 152 2109 1697
Fragments arranged by size:
2109 1697 1114 1098 739 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5689 160
Size: 872 4657 1606
Fragments arranged by size:
4657 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
AvaI C'yCGr_G
Cuts at: 1870 1943 4460 1870
Size: 73 2517 4545
Fragments arranged by size:
4545 2517 73
BamHI G'GATC_C
Cuts at: 4465 4832 4465
Size: 367 6768
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2412 4128 4456 5911 618
Size: 325 217 627 625 1716 328 1455 1842
Fragments arranged by size:
1842 1716 1455 627 625 328 325 217
BanII G_rGCy'C
Cuts at: 1391 2409 2914 3396 4329 4454 1391
Size: 1018 505 482 933 125 4072
Fragments arranged by size:
4072 1018 933 505 482 125
BbeI G_GCGC'C
Cuts at: 4132 4132
Size: 7135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2946 928
Size: 971 1047 5117
Fragments arranged by size:
5117 1047 971
BbvCI CC'TCA_GC
Cuts at: 3140 4379 3140
Size: 1239 5896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5326 5360 1903
Size: 34 3389 34 3678
Fragments arranged by size:
3678 3389 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5027 6237 5027
Size: 1210 5925
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7135
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2483 5746 136
Size: 107 122 71 631 605 811 3263 1525
Fragments arranged by size:
3263 1525 811 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7130 7130
Size: 7135
BlpI GC'TnA_GC
Cuts at: 2267 4552 2267
Size: 2285 4850
Bme1580I G_kGCm'C
Cuts at: 1790 3172 3248 5196 6352 1790
Size: 1382 76 1948 1156 2573
Fragments arranged by size:
2573 1948 1382 1156 76
BmgBI CAC'GTC
Cuts at: 1318 2111 1318
Size: 793 6342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2987 3469 3736
Size: 416 53 83 399 24 1570 482 267
Cuts at: 3736 4083 4323 4866 5824 442
Size: 347 240 543 958 1753
Fragments arranged by size:
1753 1570 958 543 482 416 399 347
267 240 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3410
Size: 7135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3527 4079 5795 1239
Size: 2288 552 1716 2579
Fragments arranged by size:
2579 2288 1716 552
Bpu10I CC'TnA_GC
Cuts at: 2408 3140 4379 2408
Size: 732 1239 5164
Fragments arranged by size:
5164 1239 732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2315 2718 2965 5260 6125 6423 2303
Size: 12 403 247 2295 865 298 3015
Fragments arranged by size:
3015 2295 865 403 298 247 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2187 2279 2592 5798 882
Size: 617 688 92 313 3206 2219
Fragments arranged by size:
3206 2219 688 617 313 92
BsaAI yAC'GTr
Cuts at: 493 1204 3871 4926 493
Size: 711 2667 1055 2702
Fragments arranged by size:
2702 2667 1055 711
BsaBI GATnn'nnATC
Cuts at: 7129 7129
Size: 7135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3120 3456 4129 4942
Size: 53 83 186 936 1587 336 673 813
Cuts at: 4942 5324 5959 275
Size: 382 635 1451
Fragments arranged by size:
1587 1451 936 813 673 635 382 336
186 83 53
BsaWI w'CCGG_w
Cuts at: 2703 2776 4165 5568 5966 6240 6387 6798 2703
Size: 73 1389 1403 398 274 147 411 3040
Fragments arranged by size:
3040 1403 1389 411 398 274 147 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3151 3181 3668 3698 1178
Size: 30 165 30 1748 30 487 30 4615
Fragments arranged by size:
4615 1748 487 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 2405 2690 3087 3258 3692 4112 1186
Size: 1198 21 285 397 171 434 420 4209
Fragments arranged by size:
4209 1198 434 420 397 285 171 21
BseYI C'CCAG_C
Cuts at: 2496 3198 3364 6338 6834 2496
Size: 702 166 2974 496 2797
Fragments arranged by size:
2974 2797 702 496 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 3672 3890 1163
Size: 573 792 1144 218 4408
Fragments arranged by size:
4408 1144 792 573 218
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4585 5346 6374 1540
Size: 275 186 2584 761 1028 2301
Fragments arranged by size:
2584 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 3033 3485 5196 5281 6352 2409
Size: 624 452 1711 85 1071 3192
Fragments arranged by size:
3192 1711 1071 624 452 85
BsmI GAATG_Cn'
Cuts at: 3789 4646 4739 3789
Size: 857 93 6185
Fragments arranged by size:
6185 857 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2914 3033 3172 3248 3396 3485
Size: 399 619 505 119 139 76 148 89
Cuts at: 3485 4329 4454 5196 5281 6352 1391
Size: 844 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 844 742 619 505 399 148
139 125 119 89 85 76
BspEI T'CCGG_A
Cuts at: 4165 6798 4165
Size: 2633 4502
BspHI T'CATG_A
Cuts at: 3760 5024 6754 3760
Size: 1264 1730 4141
Fragments arranged by size:
4141 1730 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 4490 844
Size: 1290 2356 3489
Fragments arranged by size:
3489 2356 1290
BsrBI CCG'CTC
Cuts at: 1394 1823 5022 1394
Size: 429 3199 3507
Fragments arranged by size:
3507 3199 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2882 5630 5804 65
Size: 1183 1634 2748 174 1396
Fragments arranged by size:
2748 1634 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3909 5779 5966 1700
Size: 110 2099 1870 187 2869
Fragments arranged by size:
2869 2099 1870 187 110
BsrGI T'GTAC_A
Cuts at: 96 3952 96
Size: 3856 3279
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7135
BssSI C'ACGA_G
Cuts at: 2038 5195 6207 2038
Size: 3157 1012 2966
Fragments arranged by size:
3157 2966 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4010 794
Size: 3216 3919
BstBI TT'CG_AA
Cuts at: 5939 5939
Size: 7135
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7135
BstYI r'GATC_y
Cuts at: 1972 4465 4832 5217 5234 6686 6772 6784 7130
Size: 2493 367 385 17 1452 86 12 346
Cuts at: 7130 1972
Size: 1977
Fragments arranged by size:
2493 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3588 4124 3588
Size: 536 6599
BtgI C'CryG_G
Cuts at: 513 1065 2004 3123 4039 513
Size: 552 939 1119 916 3609
Fragments arranged by size:
3609 1119 939 916 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6396
Fragments arranged by size:
6396 683 56
BtsI GCAGTG_nn'
Cuts at: 3207 3554 3657 3689 3898 4651 5445 5465 3207
Size: 347 103 32 209 753 794 20 4877
Fragments arranged by size:
4877 794 753 347 209 103 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2865 2900 529
Size: 35 2301 35 4764
Fragments arranged by size:
4764 2301 35 35
DraI TTT'AAA
Cuts at: 2833 4439 2833
Size: 1606 5529
DraIII CAC_nnn'GTG
Cuts at: 2224 3973 2224
Size: 1749 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2314 6142 809
Size: 507 998 3828 1802
Fragments arranged by size:
3828 1802 998 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3154 3562 3914
Size: 54 1425 50 275 18 1324 408 352
Cuts at: 3914 4241 4582 5471 7100 8
Size: 327 341 889 1629 43
Fragments arranged by size:
1629 1425 1324 889 408 352 341 327
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4582 1537
Size: 275 2770 4090
Fragments arranged by size:
4090 2770 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2238 2427 3821 5065 1353
Size: 689 196 189 1394 1244 3423
Fragments arranged by size:
3423 1394 1244 689 196 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3041 5722 6096 6242 387
Size: 1064 138 1452 2681 374 146 1280
Fragments arranged by size:
2681 1452 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2407 2407
Size: 7135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2262 2331 2345 2508 2841 3527
Size: 393 434 196 69 14 163 333 686
Cuts at: 3527 3927 4079 5198 5795 6582 6850 1239
Size: 400 152 1119 597 787 268 1524
Fragments arranged by size:
1524 1119 787 686 597 434 400 393
333 268 196 163 152 69 14
EcoO109I rG'GnC_Cy
Cuts at: 2052 2738 2815 3257 4375 2052
Size: 686 77 442 1118 4812
Fragments arranged by size:
4812 1118 686 442 77
EcoRI G'AATT_C
Cuts at: 4444 4822 4444
Size: 378 6757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2267 2267
Size: 7135
FspI TGC'GCA
Cuts at: 5641 5641
Size: 7135
HaeII r_GCGC'y
Cuts at: 1678 4132 6282 1678
Size: 2454 2150 2531
Fragments arranged by size:
2531 2454 2150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2554 2586 2716
Size: 32 135 32 542 32 408 32 130
Cuts at: 2716 2748 3133 3165 3215 3247 3668 3700 5776
Size: 32 385 32 50 32 421 32 2076
Cuts at: 5776 5808 5850 5882 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2076 542 421 408 385 135 130
50 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3775 3883 4479 4725 669
Size: 988 281 1837 108 596 246 3079
Fragments arranged by size:
3079 1837 988 596 281 246 108
HindIII A'AGCT_T
Cuts at: 748 3056 3638 748
Size: 2308 582 4245
Fragments arranged by size:
4245 2308 582
HpaI GTT'AAC
Cuts at: 4725 4725
Size: 7135
KasI G'GCGC_C
Cuts at: 4128 4128
Size: 7135
KpnI G_GTAC'C
Cuts at: 4460 4460
Size: 7135
MfeI C'AATT_G
Cuts at: 4734 4734
Size: 7135
MluI A'CGCG_T
Cuts at: 7011 7011
Size: 7135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 2648 3987 6249 6433 1305
Size: 1113 230 1339 2262 184 2007
Fragments arranged by size:
2262 2007 1339 1113 230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3564 10
Size: 54 1425 343 1732 3581
Fragments arranged by size:
3581 1732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2070 3212 3707 3869 5093 5452 5611
Size: 648 904 1142 495 162 1224 359 159
Cuts at: 5611 518
Size: 2042
Fragments arranged by size:
2042 1224 1142 904 648 495 359 162
159
MspA1I CmG'CkG
Cuts at: 1606 2500 3920 4551 5228 6376 6621 1606
Size: 894 1420 631 677 1148 245 2120
Fragments arranged by size:
2120 1420 1148 894 677 631 245
NarI GG'CG_CC
Cuts at: 4129 4129
Size: 7135
NcoI C'CATG_G
Cuts at: 513 1065 2004 4039 513
Size: 552 939 2035 3609
Fragments arranged by size:
3609 2035 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7135
NotI GC'GGCC_GC
Cuts at: 4582 4582
Size: 7135
NspI r_CATG'y
Cuts at: 1132 1229 3881 4493 6038 1132
Size: 97 2652 612 1545 2229
Fragments arranged by size:
2652 2229 1545 612 97
PasI CC'CwG_GG
Cuts at: 1348 2176 1348
Size: 828 6307
PciI A'CATG_T
Cuts at: 6034 6034
Size: 7135
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3549 1108
Size: 2441 4694
PfoI T'CCnGG_A
Cuts at: 2558 4056 2558
Size: 1498 5637
PmeI GTTT'AAAC
Cuts at: 4439 4439
Size: 7135
PmlI CAC'GTG
Cuts at: 3871 3871
Size: 7135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 6717 6749 5204
Size: 32 1481 32 5590
Fragments arranged by size:
5590 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2052 2815 3257 2052
Size: 763 442 5930
Fragments arranged by size:
5930 763 442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7135
PsiI TTA'TAA
Cuts at: 4705 4705
Size: 7135
PstI C_TGCA'G
Cuts at: 830 4487 830
Size: 3657 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7135
PvuII CAG'CTG
Cuts at: 2500 3920 2500
Size: 1420 5715
SacI G_AGCT'C
Cuts at: 2409 2409
Size: 7135
SalI G'TCGA_C
Cuts at: 1936 4477 1936
Size: 2541 4594
SbfI CC_TGCA'GG
Cuts at: 4487 4487
Size: 7135
ScaI AGT'ACT
Cuts at: 5383 7095 5383
Size: 1712 5423
SexAI A'CCwGG_T
Cuts at: 2220 4416 2220
Size: 2196 4939
SfcI C'TryA_G
Cuts at: 826 1045 2865 4360 4483 5618 6299 6490 826
Size: 219 1820 1495 123 1135 681 191 1471
Fragments arranged by size:
1820 1495 1471 1135 681 219 191 123
SfoI GGC'GCC
Cuts at: 4130 4130
Size: 7135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
SgrAI Cr'CCGG_yG
Cuts at: 1700 3909 1700
Size: 2209 4926
SmaI CCC'GGG
Cuts at: 1872 4462 1872
Size: 2590 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2330 2733 2944 5239 6140
Size: 197 926 339 48 403 211 2295 901
Cuts at: 6140 6402 820
Size: 262 1553
Fragments arranged by size:
2295 1553 926 901 403 339 262 211
197 48
SnaBI TAC'GTA
Cuts at: 493 4926 493
Size: 4433 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7135
SphI G_CATG'C
Cuts at: 1132 4493 1132
Size: 3361 3774
SspI AAT'ATT
Cuts at: 5 52 5059 5
Size: 47 5007 2081
Fragments arranged by size:
5007 2081 47
StuI AGG'CCT
Cuts at: 1517 4047 1517
Size: 2530 4605
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 3438 4039 4244 4574 513
Size: 552 939 1002 432 601 205 330 3074
Fragments arranged by size:
3074 1002 939 601 552 432 330 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3636 3697 4030 5162 5179 5332 5517
Size: 1255 2056 61 333 1132 17 153 185
Cuts at: 5517 5962 325
Size: 445 1498
Fragments arranged by size:
2056 1498 1255 1132 445 333 185 153
61 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3744 3952 5381
Size: 274 80 33 51 1443 1767 208 1429
Cuts at: 5381 7093 96
Size: 1712 138
Fragments arranged by size:
1767 1712 1443 1429 274 208 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2208 2240 3151 3183 7095 7127 2208
Size: 32 911 32 3912 32 2216
Fragments arranged by size:
3912 2216 911 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4337 1316
Size: 3021 4114
XbaI T'CTAG_A
Cuts at: 2113 2935 3767 4471 2113
Size: 822 832 704 4777
Fragments arranged by size:
4777 832 822 704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7135
XmaI C'CCGG_G
Cuts at: 1870 4460 1870
Size: 2590 4545
XmnI GAAnn'nnTTC
Cuts at: 2187 5264 2187
Size: 3077 4058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4943 276
Size: 53 83 186 936 3409 2468
Fragments arranged by size:
3409 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SrfI SwaI XcmI