Restriction map for
pFN21AB1338
(Circular) (Six-base) MAPSORT of: pFN21AB1338.seq Check: 1037 from: 1 to: 6520
pFN21AB1338 6520 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2380 2380
Size: 6520
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2624 4330 278
Size: 53 83 186 936 1088 1706 2468
Fragments arranged by size:
2468 1706 1088 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3863 1937
Size: 1926 4594
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2491 4583 5967 6235 1632
Size: 859 2092 1384 268 1917
Fragments arranged by size:
2092 1917 1384 859 268
AfeI AGC'GCT
Cuts at: 3517 3517
Size: 6520
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 2223 5419 6396 1203
Size: 1020 3196 977 1327
Fragments arranged by size:
3196 1327 1020 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 2959 5249 2959
Size: 2290 4230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 3247 3751 4263 5835 3247
Size: 504 512 1572 3932
Fragments arranged by size:
3932 1572 512 504
ApaI G_GGCC'C
Cuts at: 2108 3072 3094 2108
Size: 964 22 5534
Fragments arranged by size:
5534 964 22
ApaLI G'TGCA_C
Cuts at: 2045 4577 5733 2045
Size: 2532 1156 2832
Fragments arranged by size:
2832 2532 1156
ApoI r'AATT_y
Cuts at: 1493 3829 4055 4207 6316 1493
Size: 2336 226 152 2109 1697
Fragments arranged by size:
2336 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2541 3393 3410 3845 1870
Size: 73 598 852 17 435 4545
Fragments arranged by size:
4545 852 598 435 73 17
BamHI G'GATC_C
Cuts at: 2930 3850 4217 2930
Size: 920 367 5233
Fragments arranged by size:
5233 920 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 3176 3469 3625 3803
Size: 325 217 627 238 1151 293 156 178
Cuts at: 3803 3841 5296 618
Size: 38 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 293 238 217
178 156 38
BanII G_rGCy'C
Cuts at: 1391 2108 2270 2549 3039 3072 3094 3638 3770
Size: 717 162 279 490 33 22 544 132
Cuts at: 3770 3839 1391
Size: 69 4072
Fragments arranged by size:
4072 717 544 490 279 162 132 69
33 22
BbeI G_GCGC'C
Cuts at: 2029 3629 2029
Size: 1600 4920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3709 928
Size: 971 1810 3739
Fragments arranged by size:
3739 1810 971
BbvCI CC'TCA_GC
Cuts at: 3430 3430
Size: 6520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2520 3067 3609
Size: 107 122 71 631 605 848 547 542
Cuts at: 3609 5131 136
Size: 1522 1525
Fragments arranged by size:
1525 1522 848 631 605 547 542 122
107 71
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 3671 3769 3937 3671
Size: 98 168 6254
Fragments arranged by size:
6254 168 98
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2108 3072 3094 4581 5737 1790
Size: 259 59 964 22 1487 1156 2573
Fragments arranged by size:
2573 1487 1156 964 259 59 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3047 3135 3300
Size: 416 53 83 399 24 1630 88 165
Cuts at: 3300 4251 5209 442
Size: 951 958 1753
Fragments arranged by size:
1753 1630 958 951 416 399 165 88
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2621 3173 3192 3257 5180 1239
Size: 1253 129 552 19 65 1923 2579
Fragments arranged by size:
2579 1923 1253 552 129 65 19
Bpu10I CC'TnA_GC
Cuts at: 3430 3430
Size: 6520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2306 2390 2432 4645 5510 5808 2243
Size: 63 84 42 2213 865 298 2955
Fragments arranged by size:
2955 2213 865 298 84 63 42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2967 3149 3215 3288 3515 5183 882
Size: 617 1468 182 66 73 227 1668 2219
Fragments arranged by size:
2219 1668 1468 617 227 182 73 66
BsaAI yAC'GTr
Cuts at: 493 1204 2224 4311 493
Size: 711 1020 2087 2702
Fragments arranged by size:
2702 2087 1020 711
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2364 2621 3626
Size: 53 83 186 936 493 338 257 1005
Cuts at: 3626 4327 4709 5344 275
Size: 701 382 635 1451
Fragments arranged by size:
1451 1005 936 701 635 493 382 338
257 186 83 53
BsaWI w'CCGG_w
Cuts at: 4953 5351 5625 5772 6183 4953
Size: 398 274 147 411 5290
Fragments arranged by size:
5290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2522 2552 2582
Size: 30 165 30 636 30 453 30 30
Cuts at: 2582 2678 2708 1178
Size: 96 30 4990
Fragments arranged by size:
4990 636 453 165 96 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 2557 2560 2687 2804 2807 3016 3019
Size: 1243 128 3 127 117 3 209 3
Cuts at: 3019 1186
Size: 4687
Fragments arranged by size:
4687 1243 209 128 127 117 3 3
3
BseYI C'CCAG_C
Cuts at: 2500 3065 3119 3263 3299 3375 3598 5723 6219
Size: 565 54 144 36 76 223 2125 496
Cuts at: 6219 2500
Size: 2801
Fragments arranged by size:
2801 2125 565 496 223 144 76 54
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2751 1163
Size: 573 1015 4932
Fragments arranged by size:
4932 1015 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3970 4731 5759 1540
Size: 275 186 343 1626 761 1028 2301
Fragments arranged by size:
2301 1626 1028 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 3770 4581 4666 5737 2049
Size: 1721 811 85 1071 2832
Fragments arranged by size:
2832 1721 1071 811 85
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
BsmBI CGTCTCn'nnnn_
Cuts at: 3545 3545
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2108 2270 2549 3039 3072 3094
Size: 399 259 59 162 279 490 33 22
Cuts at: 3094 3638 3770 3839 4581 4666 5737 1391
Size: 544 132 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 490 399 279 259
162 132 85 69 59 33 22
BspEI T'CCGG_A
Cuts at: 6183 6183
Size: 6520
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2262 2380 2857 3875 844
Size: 1418 118 477 1018 3489
Fragments arranged by size:
3489 1418 1018 477 118
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 2419 2538 3670 4407 1394
Size: 429 452 144 119 1132 737 3507
Fragments arranged by size:
3507 1132 737 452 429 144 119
BsrDI GCAATG_nn'
Cuts at: 65 1248 2098 2240 5015 5189 65
Size: 1183 850 142 2775 174 1396
Fragments arranged by size:
2775 1396 1183 850 174 142
BsrFI r'CCGG_y
Cuts at: 1700 1810 3584 3592 3800 5164 5351 1700
Size: 110 1774 8 208 1364 187 2869
Fragments arranged by size:
2869 1774 1364 208 187 110 8
BsrGI T'GTAC_A
Cuts at: 96 2226 96
Size: 2130 4390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3247 3751 794
Size: 2453 504 3563
Fragments arranged by size:
3563 2453 504
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 2930 3007 3850 4217 4602 4619 6071 6157
Size: 958 77 843 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 958 843 385 367 346 86
77 17 12
Bsu36I CC'TnA_GG
Cuts at: 2974 2974
Size: 6520
BtgI C'CryG_G
Cuts at: 513 1065 2718 2739 2888 3779 513
Size: 552 1653 21 149 891 3254
Fragments arranged by size:
3254 1653 891 552 149 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 2497 2872 4036 4830 4850 2497
Size: 375 1164 794 20 4167
Fragments arranged by size:
4167 1164 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DraIII CAC_nnn'GTG
Cuts at: 3173 3173
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2363 3412 3461 5527 809
Size: 507 1047 1049 49 2066 1802
Fragments arranged by size:
2066 1802 1049 1047 507 49
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3967 4856
Size: 54 1425 50 275 18 891 1246 889
Cuts at: 4856 6485 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1246 891 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2578 2625 2772 4450 1353
Size: 1225 47 147 1678 3423
Fragments arranged by size:
3423 1678 1225 147 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3768 3768
Size: 6520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2491 2492 2621 3173 3192 3257 4583
Size: 393 859 1 129 552 19 65 1326
Cuts at: 4583 5180 5967 6235 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1326 859 787 597 552 393 268
129 65 19 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2603 3043 2405
Size: 198 440 5882
Fragments arranged by size:
5882 440 198
EcoO109I rG'GnC_Cy
Cuts at: 2104 2105 2694 2942 3048 3069 3091 3244 3425
Size: 1 589 248 106 21 22 153 181
Cuts at: 3425 2104
Size: 5199
Fragments arranged by size:
5199 589 248 181 153 106 22 21
1
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
FspI TGC'GCA
Cuts at: 3581 5026 3581
Size: 1445 5075
FspAI rTGC'GCAy
Cuts at: 3581 3581
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 2029 3519 3599 3629 3654 5667 1678
Size: 351 1490 80 30 25 2013 2531
Fragments arranged by size:
2531 2013 1490 351 80 30 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 2945 2977 5161
Size: 32 135 32 1106 32 235 32 2184
Cuts at: 5161 5193 5235 5267 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2184 1106 235 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2368 3448 3864 4110 669
Size: 988 281 430 1080 416 246 3079
Fragments arranged by size:
3079 1080 988 430 416 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KasI G'GCGC_C
Cuts at: 2025 3625 2025
Size: 1600 4920
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 3186 5634 5818 1305
Size: 1314 567 2448 184 2007
Fragments arranged by size:
2448 2007 1314 567 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 3807
Fragments arranged by size:
3807 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 4478 4837 4996 518
Size: 648 1011 2301 359 159 2042
Fragments arranged by size:
2301 2042 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2205 2500 2644 2650 2755 3686 3751 3781
Size: 599 295 144 6 105 931 65 30
Cuts at: 3781 3936 4613 5761 6006 1606
Size: 155 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 599 295 245 155
144 105 65 30 6
NaeI GCC'GGC
Cuts at: 3594 3594
Size: 6520
NarI GG'CG_CC
Cuts at: 2026 3626 2026
Size: 1600 4920
NcoI C'CATG_G
Cuts at: 513 1065 2718 2888 513
Size: 552 1653 170 4145
Fragments arranged by size:
4145 1653 552 170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NgoMIV G'CCGG_C
Cuts at: 3592 3592
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2182 3878 5423 1132
Size: 97 953 1696 1545 2229
Fragments arranged by size:
2229 1696 1545 953 97
PasI CC'CwG_GG
Cuts at: 1348 2100 3003 3039 3087 3631 1348
Size: 752 903 36 48 544 4237
Fragments arranged by size:
4237 903 752 544 48 36
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6520
PfoI T'CCnGG_A
Cuts at: 2823 2823
Size: 6520
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PmlI CAC'GTG
Cuts at: 2224 2224
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2694 3048 3425 2694
Size: 354 377 5789
Fragments arranged by size:
5789 377 354
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PspOMI G'GGCC_C
Cuts at: 2104 3068 3090 2104
Size: 964 22 5534
Fragments arranged by size:
5534 964 22
PstI C_TGCA'G
Cuts at: 830 2643 2649 2953 3872 830
Size: 1813 6 304 919 3478
Fragments arranged by size:
3478 1813 919 304 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2500 2644 2650 3686 2500
Size: 144 6 1036 5334
Fragments arranged by size:
5334 1036 144 6
SacI G_AGCT'C
Cuts at: 3770 3770
Size: 6520
SacII CC_GC'GG
Cuts at: 3782 3782
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SanDI GG'GwC_CC
Cuts at: 3425 3425
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SexAI A'CCwGG_T
Cuts at: 3169 3169
Size: 6520
SfcI C'TryA_G
Cuts at: 826 1045 2530 2639 2645 2949 3646 3868 5003
Size: 219 1485 109 6 304 697 222 1135
Cuts at: 5003 5684 5875 826
Size: 681 191 1471
Fragments arranged by size:
1485 1471 1135 697 681 304 222 219
191 109 6
SfoI GGC'GCC
Cuts at: 2027 3627 2027
Size: 1600 4920
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 3592 1700
Size: 1892 4628
SmaI CCC'GGG
Cuts at: 1872 2543 3847 1872
Size: 671 1304 4545
Fragments arranged by size:
4545 1304 671
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 2321 2405 2447 4624 5525
Size: 197 926 315 63 84 42 2177 901
Cuts at: 5525 5787 820
Size: 262 1553
Fragments arranged by size:
2177 1553 926 901 315 262 197 84
63 42
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 2182 3878 1132
Size: 1050 1696 3774
Fragments arranged by size:
3774 1696 1050
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6520
StyI C'CwwG_G
Cuts at: 513 1065 2454 2718 2888 3137 3225 3441 3959
Size: 552 1389 264 170 249 88 216 518
Cuts at: 3959 513
Size: 3074
Fragments arranged by size:
3074 1389 552 518 264 249 216 170
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4547 4564 4717 4902 5347 325
Size: 1255 2967 17 153 185 445 1498
Fragments arranged by size:
2967 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2334 4766
Size: 274 80 33 51 1443 249 108 2432
Cuts at: 4766 6478 96
Size: 1712 138
Fragments arranged by size:
2432 1712 1443 274 249 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 6480 6512 2039
Size: 32 4409 32 2047
Fragments arranged by size:
4409 2047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2827 3232 3271 2461
Size: 366 405 39 5710
Fragments arranged by size:
5710 405 366 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 2541 3845 1870
Size: 671 1304 4545
Fragments arranged by size:
4545 1304 671
XmnI GAAnn'nnTTC
Cuts at: 3497 4649 3497
Size: 1152 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2622 4328 276
Size: 53 83 186 936 1088 1706 2468
Fragments arranged by size:
2468 1706 1088 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I EcoRV FalI FseI NruI NsiI PacI PspXI
PsrI RsrII SapI SfiI SrfI SwaI