Restriction map for
pFN21AB1292
(Circular) (Six-base) MAPSORT of: pFN21AB1292.seq Check: 1873 from: 1 to: 6661
pFN21AB1292 6661 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3173 3173
Size: 6661
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4471 278
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 1937
Size: 2067 4594
Acc65I G'GTAC_C
Cuts at: 2396 3982 2396
Size: 1586 5075
AclI AA'CG_TT
Cuts at: 1563 4788 5161 1563
Size: 3225 373 3063
Fragments arranged by size:
3225 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4724 6108 6376 1632
Size: 3092 1384 268 1917
Fragments arranged by size:
3092 1917 1384 268
AfeI AGC'GCT
Cuts at: 3916 3916
Size: 6661
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 5560 6537 1203
Size: 4357 977 1327
Fragments arranged by size:
4357 1327 977
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 3265 5390 3265
Size: 2125 4536
AleI CACnn'nnGTG
Cuts at: 1166 3749 1166
Size: 2583 4078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2331 1184
Size: 1147 5514
AlwNI CAG_nnn'CTG
Cuts at: 4404 5976 4404
Size: 1572 5089
ApaI G_GGCC'C
Cuts at: 3104 3104
Size: 6661
ApaLI G'TGCA_C
Cuts at: 3044 3197 4718 5874 3044
Size: 153 1521 1156 3831
Fragments arranged by size:
3831 1521 1156 153
ApoI r'AATT_y
Cuts at: 1493 2502 3970 4196 4348 6457 1493
Size: 1009 1468 226 152 2109 1697
Fragments arranged by size:
2109 1697 1468 1009 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 2925 3055 3104 3304 3446 3986 1870
Size: 73 982 130 49 200 142 540 4545
Fragments arranged by size:
4545 982 540 200 142 130 73 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2097 2130 2097
Size: 33 6628
BamHI G'GATC_C
Cuts at: 3825 3945 3991 4358 3825
Size: 120 46 367 6128
Fragments arranged by size:
6128 367 120 46
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2396 2831 2990 3852
Size: 325 217 627 405 204 435 159 862
Cuts at: 3852 3982 5437 618
Size: 130 1455 1842
Fragments arranged by size:
1842 1455 862 627 435 405 325 217
204 159 130
BanII G_rGCy'C
Cuts at: 1391 2120 3063 3104 3806 3980 1391
Size: 729 943 41 702 174 4072
Fragments arranged by size:
4072 943 729 702 174 41
BbeI G_GCGC'C
Cuts at: 2196 3856 2196
Size: 1660 5001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2529 928
Size: 971 630 5060
Fragments arranged by size:
5060 971 630
BbvCI CC'TCA_GC
Cuts at: 3736 3736
Size: 6661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4852 4886 1903
Size: 34 2915 34 3678
Fragments arranged by size:
3678 2915 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4553 5763 4553
Size: 1210 5451
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6661
BfrBI ATG'CAT
Cuts at: 2148 2148
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5272 136
Size: 107 122 71 631 605 3600 1525
Fragments arranged by size:
3600 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3070 6656 3070
Size: 3586 3075
BlpI GC'TnA_GC
Cuts at: 4078 4078
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1790 2551 3048 3104 3201 4722 5878 1790
Size: 761 497 56 97 1521 1156 2573
Fragments arranged by size:
2573 1521 1156 761 497 97 56
BmgBI CAC'GTC
Cuts at: 1318 2233 3523 1318
Size: 915 1290 4456
Fragments arranged by size:
4456 1290 915
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2549 2804 2897
Size: 416 53 83 399 24 1132 255 93
Cuts at: 2897 4392 5350 442
Size: 1495 958 1753
Fragments arranged by size:
1753 1495 1132 958 416 399 255 93
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3751 3751
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5321 1239
Size: 4082 2579
Bpu10I CC'TnA_GC
Cuts at: 3736 3736
Size: 6661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 4786 5651 5949 2155
Size: 2631 865 298 2867
Fragments arranged by size:
2867 2631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5324 882
Size: 617 3825 2219
Fragments arranged by size:
3825 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4452 493
Size: 711 3248 2702
Fragments arranged by size:
3248 2702 711
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 3374 3853 4468
Size: 53 83 186 936 660 1181 479 615
Cuts at: 4468 4850 5485 275
Size: 382 635 1451
Fragments arranged by size:
1451 1181 936 660 635 615 479 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5094 5492 5766 5913 6324 5094
Size: 398 274 147 411 5431
Fragments arranged by size:
5431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2601 2631 3010 3040 1178
Size: 30 165 30 1198 30 379 30 4799
Fragments arranged by size:
4799 1198 379 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3122 3362 3434 3437 3446 3639 1186
Size: 1936 240 72 3 9 193 4208
Fragments arranged by size:
4208 1936 240 193 72 9 3
BseYI C'CCAG_C
Cuts at: 2270 3902 5864 6360 2270
Size: 1632 1962 496 2571
Fragments arranged by size:
2571 1962 1632 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 3062 3622 1163
Size: 573 693 633 560 4202
Fragments arranged by size:
4202 693 633 573 560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3726 4111 4872 5900 1540
Size: 275 186 1725 385 761 1028 2301
Fragments arranged by size:
2301 1725 1028 761 385 275 186
BsiHKAI G_wGCw'C
Cuts at: 2614 2622 2947 3048 3201 4722 4807 5878 2614
Size: 8 325 101 153 1521 85 1071 3397
Fragments arranged by size:
3397 1521 1071 325 153 101 85 8
BsmI GAATG_Cn'
Cuts at: 4172 4265 4172
Size: 93 6568
BsmBI CGTCTCn'nnnn_
Cuts at: 2243 2243
Size: 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2551 2614 2622 2947 3048 3063
Size: 399 330 431 63 8 325 101 15
Cuts at: 3063 3104 3201 3806 3980 4722 4807 5878 1391
Size: 41 97 605 174 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 605 431 399 330 325
174 101 97 85 63 41 15 8
BspEI T'CCGG_A
Cuts at: 6324 6324
Size: 6661
BspHI T'CATG_A
Cuts at: 4550 6280 4550
Size: 1730 4931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 3466 4016 844
Size: 2329 293 550 3489
Fragments arranged by size:
3489 2329 550 293
BsrBI CCG'CTC
Cuts at: 1394 1823 2863 3831 4548 1394
Size: 429 1040 968 717 3507
Fragments arranged by size:
3507 1040 968 717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3523 5156 5330 65
Size: 1183 2275 1633 174 1396
Fragments arranged by size:
2275 1633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2174 3135 5305 5492 1700
Size: 110 364 961 2170 187 2869
Fragments arranged by size:
2869 2170 961 364 187 110
BsrGI T'GTAC_A
Cuts at: 96 2241 2601 96
Size: 2145 360 4156
Fragments arranged by size:
4156 2145 360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 2763 4721 5733 2763
Size: 1958 1012 3691
Fragments arranged by size:
3691 1958 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6661
BstBI TT'CG_AA
Cuts at: 5465 5465
Size: 6661
BstEII G'GTnAC_C
Cuts at: 1184 2128 1184
Size: 944 5717
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6661
BstYI r'GATC_y
Cuts at: 1972 3070 3825 3945 3991 4358 4743 4760 6212
Size: 1098 755 120 46 367 385 17 1452
Cuts at: 6212 6298 6310 6656 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1098 755 385 367 346 120
86 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 2327 2327
Size: 6661
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2112 2286 2711 2959 3678
Size: 552 939 6 102 174 425 248 719
Cuts at: 3678 3816 513
Size: 138 3358
Fragments arranged by size:
3358 939 719 552 425 248 174 138
102 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5922
Fragments arranged by size:
5922 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 2417 4177 4971 4991 2350
Size: 67 1760 794 20 4020
Fragments arranged by size:
4020 1760 794 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 2818 3965 2818
Size: 1147 5514
DraIII CAC_nnn'GTG
Cuts at: 3043 3043
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5668 809
Size: 507 4352 1802
Fragments arranged by size:
4352 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3007 3384
Size: 54 1425 50 275 18 177 1000 377
Cuts at: 3384 3813 4108 4997 6626 8
Size: 429 295 889 1629 43
Fragments arranged by size:
1629 1425 1000 889 429 377 295 275
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2705 2935 3275 3658 3936 4591 1353
Size: 1352 230 340 383 278 655 3423
Fragments arranged by size:
3423 1352 655 383 340 278 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3347 3359 5248 5622 5768 387
Size: 1064 138 1758 12 1889 374 146 1280
Fragments arranged by size:
1889 1758 1280 1064 374 146 138 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4724 5321 6108 6376 1239
Size: 393 3092 597 787 268 1524
Fragments arranged by size:
3092 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2326 2786 2326
Size: 460 6201
EcoO109I rG'GnC_Cy
Cuts at: 2892 3100 3101 3409 3478 3498 3688 2892
Size: 208 1 308 69 20 190 5865
Fragments arranged by size:
5865 308 208 190 69 20 1
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FspI TGC'GCA
Cuts at: 5167 5167
Size: 6661
HaeII r_GCGC'y
Cuts at: 1678 2196 3856 3918 5808 1678
Size: 518 1660 62 1890 2531
Fragments arranged by size:
2531 1890 1660 518 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3010 3042 3251 3283 3287
Size: 32 135 32 1438 32 209 32 4
Cuts at: 3287 3319 3618 3650 5302 5334 5376 5408 1373
Size: 32 299 32 1652 32 42 32 2626
Fragments arranged by size:
2626 1652 1438 299 209 135 42 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4005 4251 669
Size: 988 281 2067 246 3079
Fragments arranged by size:
3079 2067 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KasI G'GCGC_C
Cuts at: 2192 3852 2192
Size: 1660 5001
KpnI G_GTAC'C
Cuts at: 2400 3986 2400
Size: 1586 5075
MfeI C'AATT_G
Cuts at: 4260 4260
Size: 6661
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 3357 3610 3786 5775 5959 1305
Size: 1704 348 253 176 1989 184 2007
Fragments arranged by size:
2007 1989 1704 348 253 184 176
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4662
Fragments arranged by size:
4662 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2231 3749 3872 4619 4978 5137
Size: 648 843 222 1518 123 747 359 159
Cuts at: 5137 518
Size: 2042
Fragments arranged by size:
2042 1518 843 747 648 359 222 159
123
MspA1I CmG'CkG
Cuts at: 1606 2300 2311 3714 4077 4754 5902 6147 1606
Size: 694 11 1403 363 677 1148 245 2120
Fragments arranged by size:
2120 1403 1148 694 677 363 245 11
NarI GG'CG_CC
Cuts at: 2193 3853 2193
Size: 1660 5001
NcoI C'CATG_G
Cuts at: 513 1065 2004 2010 2286 2959 3678 513
Size: 552 939 6 276 673 719 3496
Fragments arranged by size:
3496 939 719 673 552 276 6
NdeI CA'TA_TG
Cuts at: 387 3955 387
Size: 3568 3093
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NsiI A_TGCA'T
Cuts at: 2150 2150
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 2148 4019 5564 1132
Size: 97 919 1871 1545 2229
Fragments arranged by size:
2229 1871 1545 919 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6661
PciI A'CATG_T
Cuts at: 5560 5560
Size: 6661
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3679 1108
Size: 2571 4090
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2892 3409 3478 3498 3688 2892
Size: 517 69 20 190 5865
Fragments arranged by size:
5865 517 190 69 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6661
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PspOMI G'GGCC_C
Cuts at: 3100 3100
Size: 6661
PspXI vC'TCGA_Gb
Cuts at: 3304 3304
Size: 6661
PstI C_TGCA'G
Cuts at: 830 3418 3477 4013 830
Size: 2588 59 536 3478
Fragments arranged by size:
3478 2588 536 59
PvuI CG_AT'CG
Cuts at: 2001 3726 2001
Size: 1725 4936
PvuII CAG'CTG
Cuts at: 2311 2311
Size: 6661
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SapI GCTCTTCn'nnn_
Cuts at: 2705 2935 3936 2705
Size: 230 1001 5430
Fragments arranged by size:
5430 1001 230
SbfI CC_TGCA'GG
Cuts at: 3477 4013 3477
Size: 536 6125
ScaI AGT'ACT
Cuts at: 4909 6621 4909
Size: 1712 4949
SexAI A'CCwGG_T
Cuts at: 2827 2827
Size: 6661
SfcI C'TryA_G
Cuts at: 826 1045 2370 3414 3473 4009 5144 5825 6016
Size: 219 1325 1044 59 536 1135 681 191
Cuts at: 6016 826
Size: 1471
Fragments arranged by size:
1471 1325 1135 1044 681 536 219 191
59
SfoI GGC'GCC
Cuts at: 2194 3854 2194
Size: 1660 5001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 3988 1872
Size: 2116 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2134 3304 4765 5666 5928 820
Size: 197 926 191 1170 1461 901 262 1553
Fragments arranged by size:
1553 1461 1170 926 901 262 197 191
SnaBI TAC'GTA
Cuts at: 493 4452 493
Size: 3959 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 2148 4019 1132
Size: 1016 1871 3774
Fragments arranged by size:
3774 1871 1016
SspI AAT'ATT
Cuts at: 5 52 2163 4585 5
Size: 47 2111 2422 2081
Fragments arranged by size:
2422 2111 2081 47
StuI AGG'CCT
Cuts at: 1517 3645 1517
Size: 2128 4533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2286 2835 2959 3678 4100
Size: 552 939 6 276 549 124 719 422
Cuts at: 4100 513
Size: 3074
Fragments arranged by size:
3074 939 719 552 549 422 276 124
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 4688 4705 4858 5043 5488 325
Size: 1255 1947 1161 17 153 185 445 1498
Fragments arranged by size:
1947 1498 1255 1161 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2491 2601
Size: 274 80 33 51 1443 264 250 110
Cuts at: 2601 4907 6619 96
Size: 2306 1712 138
Fragments arranged by size:
2306 1712 1443 274 264 250 138 110
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2601 2633 6621 6653 2601
Size: 32 3988 32 2609
Fragments arranged by size:
3988 2609 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 3997 3997
Size: 6661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3541 3541
Size: 6661
XhoI C'TCGA_G
Cuts at: 1943 3304 1943
Size: 1361 5300
XmaI C'CCGG_G
Cuts at: 1870 3986 1870
Size: 2116 4545
XmnI GAAnn'nnTTC
Cuts at: 2211 3869 4790 2211
Size: 1658 921 4082
Fragments arranged by size:
4082 1658 921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4469 276
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BstZ17I EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI
PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI