Restriction map for
pFN21AB1226
(Circular) (Six-base) MAPSORT of: pFN21AB1226.seq Check: 2290 from: 1 to: 8371
pFN21AB1226 8371 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3388 3388
Size: 8371
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6181 278
Size: 53 83 186 936 4645 2468
Fragments arranged by size:
4645 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5714 1937
Size: 3777 4594
Acc65I G'GTAC_C
Cuts at: 4872 5692 4872
Size: 820 7551
AclI AA'CG_TT
Cuts at: 1563 6498 6871 1563
Size: 4935 373 3063
Fragments arranged by size:
4935 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 4121 4874 6434 7818 8086 1632
Size: 662 1827 753 1560 1384 268 1917
Fragments arranged by size:
1917 1827 1560 1384 753 662 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8174
AflIII A'CryG_T
Cuts at: 1203 3741 3960 7270 8247 1203
Size: 2538 219 3310 977 1327
Fragments arranged by size:
3310 2538 1327 977 219
AgeI A'CCGG_T
Cuts at: 3930 7202 3930
Size: 3272 5099
AhdI GACnn_n'nnGTC
Cuts at: 7100 7100
Size: 8371
AleI CACnn'nnGTG
Cuts at: 1166 2597 5405 1166
Size: 1431 2808 4132
Fragments arranged by size:
4132 2808 1431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2615 1184
Size: 1431 6940
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3018 3050 3018
Size: 32 8339
AlwNI CAG_nnn'CTG
Cuts at: 2428 2771 3234 3521 3577 4117 4135 4852 5290
Size: 343 463 287 56 540 18 717 438
Cuts at: 5290 5609 6114 7686 2428
Size: 319 505 1572 3113
Fragments arranged by size:
3113 1572 717 540 505 463 438 343
319 287 56 18
ApaI G_GGCC'C
Cuts at: 2040 2040
Size: 8371
ApaLI G'TGCA_C
Cuts at: 6428 7584 6428
Size: 1156 7215
ApoI r'AATT_y
Cuts at: 1493 3812 5680 5906 6058 8167 1493
Size: 2319 1868 226 152 2109 1697
Fragments arranged by size:
2319 2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5166 6925 160
Size: 872 4134 1759 1606
Fragments arranged by size:
4134 1759 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8371
AvaI C'yCGr_G
Cuts at: 1870 1943 4430 4675 5696 1870
Size: 73 2487 245 1021 4545
Fragments arranged by size:
4545 2487 1021 245 73
AvrII C'CTAG_G
Cuts at: 2290 2290
Size: 8371
BamHI G'GATC_C
Cuts at: 5480 5701 6068 5480
Size: 221 367 7783
Fragments arranged by size:
7783 367 221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 4511 4872 5692 7147
Size: 325 217 627 1130 1594 361 820 1455
Cuts at: 7147 618
Size: 1842
Fragments arranged by size:
1842 1594 1455 1130 820 627 361 325
217
BanII G_rGCy'C
Cuts at: 1391 2040 2325 2691 2970 4766 4926 5690 1391
Size: 649 285 366 279 1796 160 764 4072
Fragments arranged by size:
4072 1796 764 649 366 285 279 160
BbeI G_GCGC'C
Cuts at: 2921 2921
Size: 8371
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 3957 4053 4899 4989 5663 928
Size: 971 472 1586 96 846 90 674 3636
Fragments arranged by size:
3636 1586 971 846 674 472 96 90
BbvCI CC'TCA_GC
Cuts at: 2411 3198 4656 2411
Size: 787 1458 6126
Fragments arranged by size:
6126 1458 787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5437 5471 6562 6596 1903
Size: 34 50 34 3416 34 1091 34 3678
Fragments arranged by size:
3678 3416 1091 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6263 7473 6263
Size: 1210 7161
BclI T'GATC_A
Cuts at: 1548 4485 5193 1548
Size: 2937 708 4726
Fragments arranged by size:
4726 2937 708
BfrBI ATG'CAT
Cuts at: 3685 3685
Size: 8371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2627 6982 136
Size: 107 122 71 631 605 955 4355 1525
Fragments arranged by size:
4355 1525 955 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8366 8366
Size: 8371
BlpI GC'TnA_GC
Cuts at: 2072 5788 2072
Size: 3716 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2221 6432 7588 1790
Size: 250 181 4211 1156 2573
Fragments arranged by size:
4211 2573 1156 250 181
BmgBI CAC'GTC
Cuts at: 1318 2143 2980 3742 3963 1318
Size: 825 837 762 221 5726
Fragments arranged by size:
5726 837 825 762 221
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2394 2475 4279
Size: 416 53 83 399 24 977 81 1804
Cuts at: 4279 5362 6102 7060 442
Size: 1083 740 958 1753
Fragments arranged by size:
1804 1753 1083 977 958 740 416 399
83 81 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4834 4834
Size: 8371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 2806 3590 3737 3752 4302 4565 7031
Size: 1320 247 784 147 15 550 263 2466
Cuts at: 7031 1239
Size: 2579
Fragments arranged by size:
2579 2466 1320 784 550 263 247 147
15
Bpu10I CC'TnA_GC
Cuts at: 2411 3198 3578 4656 4760 2411
Size: 787 380 1078 104 6022
Fragments arranged by size:
6022 1078 787 380 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3125 3294 5042 5373 6496 7361 7659 3125
Size: 169 1748 331 1123 865 298 3837
Fragments arranged by size:
3837 1748 1123 865 331 298 169
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2824 7034 882
Size: 617 1325 4210 2219
Fragments arranged by size:
4210 2219 1325 617
BsaAI yAC'GTr
Cuts at: 493 1204 5021 6162 493
Size: 711 3817 1141 2702
Fragments arranged by size:
3817 2702 1141 711
BsaBI GATnn'nnATC
Cuts at: 3248 4385 4454 4862 8365 3248
Size: 1137 69 408 3503 3254
Fragments arranged by size:
3503 3254 1137 408 69
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2918 6178 6560 7195
Size: 53 83 186 936 1385 3260 382 635
Cuts at: 7195 275
Size: 1451
Fragments arranged by size:
3260 1451 1385 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3930 4803 6804 7202 7476 7623 8034 3930
Size: 873 2001 398 274 147 411 4267
Fragments arranged by size:
4267 2001 873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 2804 2834 3018
Size: 30 165 30 1030 30 341 30 184
Cuts at: 3018 3048 4790 4820 1178
Size: 30 1742 30 4729
Fragments arranged by size:
4729 1742 1030 341 184 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8371
BseYI C'CCAG_C
Cuts at: 2032 7574 8070 2032
Size: 5542 496 2333
Fragments arranged by size:
5542 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 3416 4130 4238 4517 4856 1163
Size: 573 599 1081 714 108 279 339 4678
Fragments arranged by size:
4678 1081 714 599 573 339 279 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2316 5821 6582 7610 1540
Size: 275 186 315 3505 761 1028 2301
Fragments arranged by size:
3505 2301 1028 761 315 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 3153 3657 6432 6517 7588 2325
Size: 828 504 2775 85 1071 3108
Fragments arranged by size:
3108 2775 1071 828 504 85
BsmI GAATG_Cn'
Cuts at: 4740 5882 5975 4740
Size: 1142 93 7136
Fragments arranged by size:
7136 1142 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2221 2325 2691 2970 3153 3657
Size: 399 250 181 104 366 279 183 504
Cuts at: 3657 4766 4926 5690 6432 6517 7588 1391
Size: 1109 160 764 742 85 1071 2174
Fragments arranged by size:
2174 1109 1071 764 742 504 399 366
279 250 183 181 160 104 85
BspEI T'CCGG_A
Cuts at: 8034 8034
Size: 8371
BspHI T'CATG_A
Cuts at: 6260 7990 6260
Size: 1730 6641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3388 5726 844
Size: 2544 2338 3489
Fragments arranged by size:
3489 2544 2338
BsrBI CCG'CTC
Cuts at: 1394 1823 2076 5086 6258 1394
Size: 429 253 3010 1172 3507
Fragments arranged by size:
3507 3010 1172 429 253
BsrDI GCAATG_nn'
Cuts at: 65 1248 2761 4186 5487 6866 7040 65
Size: 1183 1513 1425 1301 1379 174 1396
Fragments arranged by size:
1513 1425 1396 1379 1301 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 3930 5522 5571 7015 7202 1700
Size: 110 276 1844 1592 49 1444 187 2869
Fragments arranged by size:
2869 1844 1592 1444 276 187 110 49
BsrGI T'GTAC_A
Cuts at: 96 2178 3499 3903 96
Size: 2082 1321 404 4564
Fragments arranged by size:
4564 2082 1321 404
BssHII G'CGCG_C
Cuts at: 1925 5589 1925
Size: 3664 4707
BssSI C'ACGA_G
Cuts at: 5446 6431 7443 5446
Size: 985 1012 6374
Fragments arranged by size:
6374 1012 985
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4117 794
Size: 3323 5048
BstBI TT'CG_AA
Cuts at: 7175 7175
Size: 8371
BstEII G'GTnAC_C
Cuts at: 1184 2047 3169 3331 4555 1184
Size: 863 1122 162 1224 5000
Fragments arranged by size:
5000 1224 1122 863 162
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2333 3808 4010 2011
Size: 322 1475 202 6372
Fragments arranged by size:
6372 1475 322 202
BstYI r'GATC_y
Cuts at: 1972 2481 4386 4422 5363 5480 5701 6068 6453
Size: 509 1905 36 941 117 221 367 385
Cuts at: 6453 6470 7922 8008 8020 8366 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1905 1452 941 509 385 367 346
221 117 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 3411 3411
Size: 8371
BtgI C'CryG_G
Cuts at: 513 1065 2040 2055 2316 3336 4390 4941 513
Size: 552 975 15 261 1020 1054 551 3943
Fragments arranged by size:
3943 1054 1020 975 552 551 261 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5455 487
Size: 683 56 4229 3403
Fragments arranged by size:
4229 3403 683 56
BtsI GCAGTG_nn'
Cuts at: 4596 5309 5412 5887 6681 6701 4596
Size: 713 103 475 794 20 6266
Fragments arranged by size:
6266 794 713 475 103 20
ClaI AT'CG_AT
Cuts at: 1554 5133 1554
Size: 3579 4792
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8336
DraI TTT'AAA
Cuts at: 2527 3370 5675 2527
Size: 843 2305 5223
Fragments arranged by size:
5223 2305 843
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3571 4057 7378 809
Size: 507 2255 486 3321 1802
Fragments arranged by size:
3321 2255 1802 507 486
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 4768 5281
Size: 54 1425 50 275 18 798 2140 513
Cuts at: 5281 5520 5596 5818 6707 8336 8
Size: 239 76 222 889 1629 43
Fragments arranged by size:
2140 1629 1425 889 798 513 275 239
222 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5818 1537
Size: 275 4006 4090
Fragments arranged by size:
4090 4006 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4995 6301 1353
Size: 3642 1306 3423
Fragments arranged by size:
3642 3423 1306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2523 3089 5680 6958 7332 7478
Size: 1064 138 934 566 2591 1278 374 146
Cuts at: 7478 387
Size: 1280
Fragments arranged by size:
2591 1280 1278 1064 934 566 374 146
138
EcoICRI GAG'CTC
Cuts at: 2323 2323
Size: 8371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2559 2806 3590 3737 3752 4121
Size: 393 662 265 247 784 147 15 369
Cuts at: 4121 4302 4565 4874 6434 7031 7818 8086 1239
Size: 181 263 309 1560 597 787 268 1524
Fragments arranged by size:
1560 1524 787 784 662 597 393 369
309 268 265 263 247 181 147 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2289 2289
Size: 8371
EcoO109I rG'GnC_Cy
Cuts at: 2036 2407 2432 4094 4244 2036
Size: 371 25 1662 150 6163
Fragments arranged by size:
6163 1662 371 150 25
EcoRI G'AATT_C
Cuts at: 5680 6058 5680
Size: 378 7993
EcoRV GAT'ATC
Cuts at: 3246 3246
Size: 8371
FspI TGC'GCA
Cuts at: 6877 6877
Size: 8371
HaeII r_GCGC'y
Cuts at: 1678 2921 5093 7518 1678
Size: 1243 2172 2425 2531
Fragments arranged by size:
2531 2425 2172 1243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 2979 3011 3334
Size: 32 135 32 697 32 678 32 323
Cuts at: 3334 3366 3442 3474 3728 3760 4275 4307 5425
Size: 32 76 32 254 32 515 32 1118
Cuts at: 5425 5457 7012 7044 7086 7118 1373
Size: 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 1118 697 678 515 323 254
135 76 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3625 3874 5565 5715 5961 669
Size: 988 281 1687 249 1691 150 246 3079
Fragments arranged by size:
3079 1691 1687 988 281 249 246 150
HindIII A'AGCT_T
Cuts at: 748 4639 5231 748
Size: 3891 592 3888
Fragments arranged by size:
3891 3888 592
HpaI GTT'AAC
Cuts at: 5961 5961
Size: 8371
KasI G'GCGC_C
Cuts at: 2917 2917
Size: 8371
KpnI G_GTAC'C
Cuts at: 4876 5696 4876
Size: 820 7551
MfeI C'AATT_G
Cuts at: 5970 5970
Size: 8371
MluI A'CGCG_T
Cuts at: 8247 8247
Size: 8371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2591 3221 4416 4740 7485 7669 1305
Size: 813 473 630 1195 324 2745 184 2007
Fragments arranged by size:
2745 2007 1195 813 630 473 324 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4770 5283 10
Size: 54 1425 343 2938 513 3098
Fragments arranged by size:
3098 2938 1425 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2111 2331 2597 5405 6329 6688
Size: 648 843 102 220 266 2808 924 359
Cuts at: 6688 6847 518
Size: 159 2042
Fragments arranged by size:
2808 2042 924 843 648 359 266 220
159 102
MspA1I CmG'CkG
Cuts at: 1606 2042 2057 3202 4135 4483 5787 6464 7612
Size: 436 15 1145 933 348 1304 677 1148
Cuts at: 7612 7857 1606
Size: 245 2120
Fragments arranged by size:
2120 1304 1148 1145 933 677 436 348
245 15
NaeI GCC'GGC
Cuts at: 2088 5573 2088
Size: 3485 4886
NarI GG'CG_CC
Cuts at: 2918 2918
Size: 8371
NcoI C'CATG_G
Cuts at: 513 1065 4941 513
Size: 552 3876 3943
Fragments arranged by size:
3943 3876 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8371
NgoMIV G'CCGG_C
Cuts at: 2086 5571 2086
Size: 3485 4886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8371
NotI GC'GGCC_GC
Cuts at: 5818 5818
Size: 8371
NsiI A_TGCA'T
Cuts at: 3687 3687
Size: 8371
NspI r_CATG'y
Cuts at: 1132 1229 3778 5729 7274 1132
Size: 97 2549 1951 1545 2229
Fragments arranged by size:
2549 2229 1951 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2428 4370 1348
Size: 1080 1942 5349
Fragments arranged by size:
5349 1942 1080
PciI A'CATG_T
Cuts at: 7270 7270
Size: 8371
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2771 2983 1108
Size: 1663 212 6496
Fragments arranged by size:
6496 1663 212
PfoI T'CCnGG_A
Cuts at: 3261 3261
Size: 8371
PmeI GTTT'AAAC
Cuts at: 5675 5675
Size: 8371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 3018 3050 3929 3961 4994 5026 6440
Size: 32 29 32 879 32 1033 32 1414
Cuts at: 6440 6472 7953 7985 2957
Size: 32 1481 32 3343
Fragments arranged by size:
3343 1481 1414 1033 879 32 32 32
32 32 32 29
PpuMI rG'GwC_Cy
Cuts at: 2407 2432 2407
Size: 25 8346
PshAI GACnn'nnGTC
Cuts at: 1613 2793 1613
Size: 1180 7191
PsiI TTA'TAA
Cuts at: 3855 5941 3855
Size: 2086 6285
PspOMI G'GGCC_C
Cuts at: 2036 2036
Size: 8371
PstI C_TGCA'G
Cuts at: 830 3751 4591 4970 5562 5723 830
Size: 2921 840 379 592 161 3478
Fragments arranged by size:
3478 2921 840 592 379 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8371
PvuII CAG'CTG
Cuts at: 3202 4135 3202
Size: 933 7438
SacI G_AGCT'C
Cuts at: 2325 2325
Size: 8371
SacII CC_GC'GG
Cuts at: 2043 2058 2043
Size: 15 8356
SalI G'TCGA_C
Cuts at: 1936 5713 1936
Size: 3777 4594
SbfI CC_TGCA'GG
Cuts at: 5723 5723
Size: 8371
ScaI AGT'ACT
Cuts at: 6619 8331 6619
Size: 1712 6659
SfcI C'TryA_G
Cuts at: 826 1045 3569 3747 4516 4587 4966 5558 5576
Size: 219 2524 178 769 71 379 592 18
Cuts at: 5576 5719 6854 7535 7726 826
Size: 143 1135 681 191 1471
Fragments arranged by size:
2524 1471 1135 769 681 592 379 219
191 178 143 71 18
SfoI GGC'GCC
Cuts at: 2919 2919
Size: 8371
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8371
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8371
SmaI CCC'GGG
Cuts at: 1872 4677 5698 1872
Size: 2805 1021 4545
Fragments arranged by size:
4545 2805 1021
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3104 3309 5057 5388 6475 7376
Size: 197 926 1161 205 1748 331 1087 901
Cuts at: 7376 7638 820
Size: 262 1553
Fragments arranged by size:
1748 1553 1161 1087 926 901 331 262
205 197
SnaBI TAC'GTA
Cuts at: 493 6162 493
Size: 5669 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8371
SphI G_CATG'C
Cuts at: 1132 5729 1132
Size: 4597 3774
SspI AAT'ATT
Cuts at: 5 52 6295 5
Size: 47 6243 2081
Fragments arranged by size:
6243 2081 47
StuI AGG'CCT
Cuts at: 1517 4404 4619 1517
Size: 2887 215 5269
Fragments arranged by size:
5269 2887 215
StyI C'CwwG_G
Cuts at: 513 1065 2290 4009 4083 4941 5278 5810 513
Size: 552 1225 1719 74 858 337 532 3074
Fragments arranged by size:
3074 1719 1225 858 552 532 337 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 3485 6398 6415 6568 6753 7198
Size: 1255 906 999 2913 17 153 185 445
Cuts at: 7198 325
Size: 1498
Fragments arranged by size:
2913 1498 1255 999 906 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3499 3903
Size: 274 80 33 51 1443 201 1321 404
Cuts at: 3903 4548 6617 8329 96
Size: 645 2069 1712 138
Fragments arranged by size:
2069 1712 1443 1321 645 404 274 201
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2433 2465 2863 2895 8331 8363 2433
Size: 32 398 32 5436 32 2441
Fragments arranged by size:
5436 2441 398 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3269 1316
Size: 1953 6418
XbaI T'CTAG_A
Cuts at: 5707 5707
Size: 8371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4007 4948 4007
Size: 941 7430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8371
XmaI C'CCGG_G
Cuts at: 1870 4675 5696 1870
Size: 2805 1021 4545
Fragments arranged by size:
4545 2805 1021
XmnI GAAnn'nnTTC
Cuts at: 4799 5211 6500 4799
Size: 412 1289 6670
Fragments arranged by size:
6670 1289 412
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6179 276
Size: 53 83 186 936 4645 2468
Fragments arranged by size:
4645 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BsiWI BsmBI BstZ17I DraIII FalI
FseI FspAI NruI PacI PmlI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SrfI SwaI