Restriction map for
pFN21AB1123
(Circular) (Six-base) MAPSORT of: pFN21AB1123.seq Check: 531 from: 1 to: 12352
pFN21AB1123 12352 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3424 3887 4507 3424
Size: 463 620 11269
Fragments arranged by size:
11269 620 463
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7870 10162 278
Size: 53 83 186 936 6334 2292 2468
Fragments arranged by size:
6334 2468 2292 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2183 7949 9695 1937
Size: 246 5766 1746 4594
Fragments arranged by size:
5766 4594 1746 246
Acc65I G'GTAC_C
Cuts at: 7902 9673 7902
Size: 1771 10581
AclI AA'CG_TT
Cuts at: 1563 10479 10852 1563
Size: 8916 373 3063
Fragments arranged by size:
8916 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4352 4640 5400 5900 6078 8009 8219 10415
Size: 2720 288 760 500 178 1931 210 2196
Cuts at: 10415 11799 12067 1632
Size: 1384 268 1917
Fragments arranged by size:
2720 2196 1931 1917 1384 760 500 288
268 210 178
AfeI AGC'GCT
Cuts at: 4049 5409 5627 4049
Size: 1360 218 10774
Fragments arranged by size:
10774 1360 218
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12155
AflIII A'CryG_T
Cuts at: 1203 7113 7995 11251 12228 1203
Size: 5910 882 3256 977 1327
Fragments arranged by size:
5910 3256 1327 977 882
AgeI A'CCGG_T
Cuts at: 11183 11183
Size: 12352
AhdI GACnn_n'nnGTC
Cuts at: 5533 11081 5533
Size: 5548 6804
AleI CACnn'nnGTG
Cuts at: 1166 3071 3560 6688 1166
Size: 1905 489 3128 6830
Fragments arranged by size:
6830 3128 1905 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2094 3003 3482 6164 6706 6997 8825 1184
Size: 910 909 479 2682 542 291 1828 4711
Fragments arranged by size:
4711 2682 1828 910 909 542 479 291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5024 5056 6198 6230 5024
Size: 32 1142 32 11146
Fragments arranged by size:
11146 1142 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2884 4004 4367 4972 5050 7231 8197 10095 11667
Size: 1120 363 605 78 2181 966 1898 1572
Cuts at: 11667 2884
Size: 3569
Fragments arranged by size:
3569 2181 1898 1572 1120 966 605 363
78
ApaI G_GGCC'C
Cuts at: 5920 6116 8189 5920
Size: 196 2073 10083
Fragments arranged by size:
10083 2073 196
ApaLI G'TGCA_C
Cuts at: 10409 11565 10409
Size: 1156 11196
ApoI r'AATT_y
Cuts at: 1493 2441 3110 3782 4612 5033 5123 5201 5571
Size: 948 669 672 830 421 90 78 370
Cuts at: 5571 6089 9372 9661 9887 10039 12148 1493
Size: 518 3283 289 226 152 2109 1697
Fragments arranged by size:
3283 2109 1697 948 830 672 669 518
421 370 289 226 152 90 78
AseI AT'TA_AT
Cuts at: 160 1032 4340 6261 10906 160
Size: 872 3308 1921 4645 1606
Fragments arranged by size:
4645 3308 1921 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12352
AvaI C'yCGr_G
Cuts at: 1870 1943 3474 4731 6455 6615 6830 8704 9105
Size: 73 1531 1257 1724 160 215 1874 401
Cuts at: 9105 9677 1870
Size: 572 4545
Fragments arranged by size:
4545 1874 1724 1531 1257 572 401 215
160 73
BamHI G'GATC_C
Cuts at: 2784 4687 5686 9682 10049 2784
Size: 1903 999 3996 367 5087
Fragments arranged by size:
5087 3996 1903 999 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4278 5315 7648 7902 9639
Size: 325 217 627 2491 1037 2333 254 1737
Cuts at: 9639 9673 11128 618
Size: 34 1455 1842
Fragments arranged by size:
2491 2333 1842 1737 1455 1037 627 325
254 217 34
BanII G_rGCy'C
Cuts at: 1391 2136 2461 3525 4575 5504 5920 6015 6116
Size: 745 325 1064 1050 929 416 95 101
Cuts at: 6116 8163 8189 9671 1391
Size: 2047 26 1482 4072
Fragments arranged by size:
4072 2047 1482 1064 1050 929 745 416
325 101 95 26
BbeI G_GCGC'C
Cuts at: 5319 5319
Size: 12352
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3442 4628 5239 5529 6954 928
Size: 971 1543 1186 611 290 1425 6326
Fragments arranged by size:
6326 1543 1425 1186 971 611 290
BbvCI CC'TCA_GC
Cuts at: 7824 8352 8663 7824
Size: 528 311 11513
Fragments arranged by size:
11513 528 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2668 2702 3964 3998 10543
Size: 34 50 34 647 34 1262 34 6545
Cuts at: 10543 10577 1903
Size: 34 3678
Fragments arranged by size:
6545 3678 1262 647 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5654 7702 10244 11454 5654
Size: 2048 2542 1210 6552
Fragments arranged by size:
6552 2542 2048 1210
BclI T'GATC_A
Cuts at: 1548 3115 4386 1548
Size: 1567 1271 9514
Fragments arranged by size:
9514 1567 1271
BfrBI ATG'CAT
Cuts at: 5254 5735 5254
Size: 481 11871
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3669 6386 6539
Size: 107 122 71 631 605 1997 2717 153
Cuts at: 6539 6548 6984 7469 7505 8715 9183 10963 136
Size: 9 436 485 36 1210 468 1780 1525
Fragments arranged by size:
2717 1997 1780 1525 1210 631 605 485
468 436 153 122 107 71 36 9
BglII A'GATC_T
Cuts at: 2908 6855 8575 12347 2908
Size: 3947 1720 3772 2913
Fragments arranged by size:
3947 3772 2913 1720
BlpI GC'TnA_GC
Cuts at: 3971 5864 9769 3971
Size: 1893 3905 6554
Fragments arranged by size:
6554 3905 1893
Bme1580I G_kGCm'C
Cuts at: 1790 4267 4281 5920 6116 7129 8189 9644 10413
Size: 2477 14 1639 196 1013 1060 1455 769
Cuts at: 10413 11569 1790
Size: 1156 2573
Fragments arranged by size:
2573 2477 1639 1455 1156 1060 1013 769
196 14
BmgBI CAC'GTC
Cuts at: 1318 5782 1318
Size: 4464 7888
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2357 3045 3229
Size: 416 53 83 399 24 940 688 184
Cuts at: 3229 10083 11041 442
Size: 6854 958 1753
Fragments arranged by size:
6854 1753 958 940 688 416 399 184
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2280 1056
Size: 1224 11128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9542 9542
Size: 12352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 3539 4472 5891 6230 6353 6821 7688
Size: 881 1419 933 1419 339 123 468 867
Cuts at: 7688 8177 9310 11012 1239
Size: 489 1133 1702 2579
Fragments arranged by size:
2579 1702 1419 1419 1133 933 881 867
489 468 339 123
Bpu10I CC'TnA_GC
Cuts at: 3266 3575 5714 7824 8352 8663 3266
Size: 309 2139 2110 528 311 6955
Fragments arranged by size:
6955 2139 2110 528 311 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4619 6950 10477 11342 11640 4619
Size: 2331 3527 865 298 5331
Fragments arranged by size:
5331 3527 2331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 4291 4878 5833 6518 11015 882
Size: 617 1239 1553 587 955 685 4497 2219
Fragments arranged by size:
4497 2219 1553 1239 955 685 617 587
BsaAI yAC'GTr
Cuts at: 493 1204 2437 10143 493
Size: 711 1233 7706 2702
Fragments arranged by size:
7706 2702 1233 711
BsaBI GATnn'nnATC
Cuts at: 4205 4874 12346 4205
Size: 669 7472 4211
Fragments arranged by size:
7472 4211 669
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3441 4034 5316 7867
Size: 53 83 186 936 1908 593 1282 2551
Cuts at: 7867 10159 10541 11176 275
Size: 2292 382 635 1451
Fragments arranged by size:
2551 2292 1908 1451 1282 936 635 593
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2015 8821 10785 11183 11457 11604 12015 2015
Size: 6806 1964 398 274 147 411 2352
Fragments arranged by size:
6806 2352 1964 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3068 3098 4271 4301 5444
Size: 30 165 30 1665 30 1173 30 1143
Cuts at: 5444 5474 6659 6689 1178
Size: 30 1185 30 6841
Fragments arranged by size:
6841 1665 1185 1173 1143 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2336 3119 5696 6818 8060 1186
Size: 1006 144 783 2577 1122 1242 5478
Fragments arranged by size:
5478 2577 1242 1122 1006 783 144
BseYI C'CCAG_C
Cuts at: 2470 2667 2976 3004 4273 4846 5050 5220 5554
Size: 197 309 28 1269 573 204 170 334
Cuts at: 5554 5913 6151 6436 7188 8718 8888 8927 11555
Size: 359 238 285 752 1530 170 39 2628
Cuts at: 11555 12051 2470
Size: 496 2771
Fragments arranged by size:
2771 2628 1530 1269 752 573 496 359
334 309 285 238 204 197 170 170
39 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4126 4985 5762 6347 7217 8278 8726
Size: 573 2390 859 777 585 870 1061 448
Cuts at: 8726 9050 1163
Size: 324 4465
Fragments arranged by size:
4465 2390 1061 870 859 777 585 573
448 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6294 7354 7509 9802 10563 11591
Size: 275 186 4293 1060 155 2293 761 1028
Cuts at: 11591 1540
Size: 2301
Fragments arranged by size:
4293 2301 2293 1060 1028 761 275 186
155
BsiHKAI G_wGCw'C
Cuts at: 2136 3567 3849 4233 4575 5156 5640 6015 7307
Size: 1431 282 384 342 581 484 375 1292
Cuts at: 7307 7350 7571 8007 9234 10413 10498 11569 2136
Size: 43 221 436 1227 1179 85 1071 2919
Fragments arranged by size:
2919 1431 1292 1227 1179 1071 581 484
436 384 375 342 282 221 85 43
BsmI GAATG_Cn'
Cuts at: 2308 3206 8460 9863 9956 2308
Size: 898 5254 1403 93 4704
Fragments arranged by size:
5254 4704 1403 898 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5851 7053 7874 9450 5851
Size: 1202 821 1576 8753
Fragments arranged by size:
8753 1576 1202 821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2461 3525 3567 3849 4233 4267
Size: 399 346 325 1064 42 282 384 34
Cuts at: 4267 4281 4575 5156 5504 5640 5920 6015 6116
Size: 14 294 581 348 136 280 95 101
Cuts at: 6116 7129 7307 7350 7571 8007 8163 8189 9234
Size: 1013 178 43 221 436 156 26 1045
Cuts at: 9234 9644 9671 10413 10498 11569 1391
Size: 410 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 1064 1045 1013 742 581 436
410 399 384 348 346 325 294 282
280 221 178 156 136 101 95 85
43 42 34 27 26 14
BspEI T'CCGG_A
Cuts at: 8821 12015 8821
Size: 3194 9158
BspHI T'CATG_A
Cuts at: 5115 5133 6943 9547 10241 11971 5115
Size: 18 1810 2604 694 1730 5496
Fragments arranged by size:
5496 2604 1810 1730 694 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 3424 3887 4507 5223 5343 6720 9707
Size: 1185 1395 463 620 716 120 1377 2987
Cuts at: 9707 844
Size: 3489
Fragments arranged by size:
3489 2987 1395 1377 1185 716 620 463
120
BsrBI CCG'CTC
Cuts at: 1394 1823 3386 10239 1394
Size: 429 1563 6853 3507
Fragments arranged by size:
6853 3507 1563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 2491 3435 3486 6741 7830 10847
Size: 1183 814 429 944 51 3255 1089 3017
Cuts at: 10847 11021 65
Size: 174 1396
Fragments arranged by size:
3255 3017 1396 1183 1089 944 814 429
174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 3081 10996 11183 1700
Size: 110 1271 7915 187 2869
Fragments arranged by size:
7915 2869 1271 187 110
BsrGI T'GTAC_A
Cuts at: 96 2968 7641 96
Size: 2872 4673 4807
Fragments arranged by size:
4807 4673 2872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12352
BssSI C'ACGA_G
Cuts at: 6365 7689 10412 11424 6365
Size: 1324 2723 1012 7293
Fragments arranged by size:
7293 2723 1324 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4972 5344 5710 6450 7231 794
Size: 4178 372 366 740 781 5915
Fragments arranged by size:
5915 4178 781 740 372 366
BstBI TT'CG_AA
Cuts at: 5485 7398 11156 5485
Size: 1913 3758 6681
Fragments arranged by size:
6681 3758 1913
BstEII G'GTnAC_C
Cuts at: 1184 7090 1184
Size: 5906 6446
BstXI CCAn_nnnn'nTGG
Cuts at: 3073 3685 3739 5312 5524 7858 9163 3073
Size: 612 54 1573 212 2334 1305 6262
Fragments arranged by size:
6262 2334 1573 1305 612 212 54
BstYI r'GATC_y
Cuts at: 1972 2784 2908 3855 4000 4122 4357 4687 4875
Size: 812 124 947 145 122 235 330 188
Cuts at: 4875 5686 5950 6336 6855 7618 8575 8778 9060
Size: 811 264 386 519 763 957 203 282
Cuts at: 9060 9682 10049 10434 10451 11903 11989 12001 12347
Size: 622 367 385 17 1452 86 12 346
Cuts at: 12347 1972
Size: 1977
Fragments arranged by size:
1977 1452 957 947 812 811 763 622
519 386 385 367 346 330 282 264
235 203 188 145 124 122 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3726 7086 3726
Size: 3360 8992
BtgI C'CryG_G
Cuts at: 513 1065 2151 2406 2788 4857 4911 5523 6771
Size: 552 1086 255 382 2069 54 612 1248
Cuts at: 6771 7503 513
Size: 732 5362
Fragments arranged by size:
5362 2069 1248 1086 732 612 552 382
255 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3750 5877 6037 7183 7452 7575
Size: 683 56 2524 2127 160 1146 269 123
Cuts at: 7575 8783 487
Size: 1208 4056
Fragments arranged by size:
4056 2524 2127 1208 1146 683 269 160
123 56
BtsI GCAGTG_nn'
Cuts at: 3540 3555 3567 3954 5387 6497 6656 7392 7491
Size: 15 12 387 1433 1110 159 736 99
Cuts at: 7491 7512 7524 8738 9868 10662 10682 3540
Size: 21 12 1214 1130 794 20 5210
Fragments arranged by size:
5210 1433 1214 1130 1110 794 736 387
159 99 21 20 15 12 12
ClaI AT'CG_AT
Cuts at: 1554 7137 1554
Size: 5583 6769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2296 2331 3417 3452 529
Size: 35 1732 35 1086 35 9429
Fragments arranged by size:
9429 1732 1086 35 35 35
DraI TTT'AAA
Cuts at: 9656 9656
Size: 12352
DraIII CAC_nnn'GTG
Cuts at: 4705 9211 4705
Size: 4506 7846
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2366 3446 11359 809
Size: 507 1050 1080 7913 1802
Fragments arranged by size:
7913 1802 1080 1050 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2395 2529
Size: 54 1425 50 275 18 438 127 134
Cuts at: 2529 2592 2751 3919 4134 4506 4548 5722 5943
Size: 63 159 1168 215 372 42 1174 221
Cuts at: 5943 6291 7506 7751 8061 8631 8901 9249 9358
Size: 348 1215 245 310 570 270 348 109
Cuts at: 9358 9799 10688 12317 8
Size: 441 889 1629 43
Fragments arranged by size:
1629 1425 1215 1174 1168 889 570 441
438 372 348 348 310 275 270 245
221 215 159 134 127 109 63 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 6291 7506 9799 1537
Size: 275 4479 1215 2293 4090
Fragments arranged by size:
4479 4090 2293 1215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5069 5673 5960 6677 7115 8094 8612 8655
Size: 3716 604 287 717 438 979 518 43
Cuts at: 8655 10282 1353
Size: 1627 3423
Fragments arranged by size:
3716 3423 1627 979 717 604 518 438
287 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4088 4298 6839 10939 11313 11459
Size: 1064 138 2499 210 2541 4100 374 146
Cuts at: 11459 387
Size: 1280
Fragments arranged by size:
4100 2541 2499 1280 1064 374 210 146
138
EcoICRI GAG'CTC
Cuts at: 2134 4573 6013 2134
Size: 2439 1440 8473
Fragments arranged by size:
8473 2439 1440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3539 4352 4472 4640 5400 5891
Size: 393 488 1419 813 120 168 760 491
Cuts at: 5891 5900 6078 6230 6353 6821 7688 8009 8177
Size: 9 178 152 123 468 867 321 168
Cuts at: 8177 8219 9310 10415 11012 11799 12067 1239
Size: 42 1091 1105 597 787 268 1524
Fragments arranged by size:
1524 1419 1105 1091 867 813 787 760
597 491 488 468 393 321 268 178
168 168 152 123 120 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3725 4067 4976 5801 5924 8099 3725
Size: 342 909 825 123 2175 7978
Fragments arranged by size:
7978 2175 909 825 342 123
EcoO109I rG'GnC_Cy
Cuts at: 2602 3042 3682 5762 5917 6112 6113 6224 6667
Size: 440 640 2080 155 195 1 111 443
Cuts at: 6667 6709 7915 8185 8186 8290 8308 8407 8508
Size: 42 1206 270 1 104 18 99 101
Cuts at: 8508 9478 2602
Size: 970 5476
Fragments arranged by size:
5476 2080 1206 970 640 443 440 270
195 155 111 104 101 99 42 18
1 1
EcoRI G'AATT_C
Cuts at: 5123 6089 9661 10039 5123
Size: 966 3572 378 7436
Fragments arranged by size:
7436 3572 966 378
EcoRV GAT'ATC
Cuts at: 3505 3505
Size: 12352
FspI TGC'GCA
Cuts at: 4196 10858 4196
Size: 6662 5690
HaeII r_GCGC'y
Cuts at: 1678 4051 5319 5411 5629 5817 7338 11499 1678
Size: 2373 1268 92 218 188 1521 4161 2531
Fragments arranged by size:
4161 2531 2373 1521 1268 218 188 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4641 4673 5444
Size: 32 135 32 2413 32 624 32 771
Cuts at: 5444 5476 5519 5551 5598 5630 5876 5908 6659
Size: 32 43 32 47 32 246 32 751
Cuts at: 6659 6691 6739 6771 6828 6831 6860 6863 7607
Size: 32 48 32 57 3 29 3 744
Cuts at: 7607 7639 8049 8081 8630 8662 8667 8699 9335
Size: 32 410 32 549 32 5 32 636
Cuts at: 9335 9367 10993 11025 11067 11099 1373
Size: 32 1626 32 42 32 2626
Fragments arranged by size:
2626 2413 1626 771 751 744 636 624
549 410 246 135 57 48 47 43
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 29 5 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4813 6479 8620 9153 9696 9942
Size: 988 281 2875 1666 2141 533 543 246
Cuts at: 9942 669
Size: 3079
Fragments arranged by size:
3079 2875 2141 1666 988 543 533 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12352
HpaI GTT'AAC
Cuts at: 8620 9942 8620
Size: 1322 11030
KasI G'GCGC_C
Cuts at: 5315 5315
Size: 12352
KpnI G_GTAC'C
Cuts at: 7906 9677 7906
Size: 1771 10581
MfeI C'AATT_G
Cuts at: 9951 9951
Size: 12352
MluI A'CGCG_T
Cuts at: 12228 12228
Size: 12352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3235 4206 8353 9054 9593 11466 11650 1305
Size: 1930 971 4147 701 539 1873 184 2007
Fragments arranged by size:
4147 2007 1930 1873 971 701 539 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 2531 2753 3921 4136
Size: 54 1425 343 438 261 222 1168 215
Cuts at: 4136 4508 4550 5724 5945 7753 8063 8633 8903
Size: 372 42 1174 221 1808 310 570 270
Cuts at: 8903 9360 10
Size: 457 3002
Fragments arranged by size:
3002 1808 1425 1174 1168 570 457 438
372 343 310 270 261 222 221 215
54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2375 2588 3071 3464 3560 3927
Size: 648 1122 87 213 483 393 96 367
Cuts at: 3927 4481 4661 4715 5114 5522 5582 6605 6688
Size: 554 180 54 399 408 60 1023 83
Cuts at: 6688 8036 8987 9212 9251 10310 10669 10828 518
Size: 1348 951 225 39 1059 359 159 2042
Fragments arranged by size:
2042 1348 1122 1059 1023 951 648 554
483 408 399 393 367 359 225 213
180 159 96 87 83 60 54 39
MspA1I CmG'CkG
Cuts at: 1606 2039 2429 2507 2884 3299 3697 4850 5050
Size: 433 390 78 377 415 398 1153 200
Cuts at: 5050 5224 5308 5554 6290 6560 7279 7359 7467
Size: 174 84 246 736 270 719 80 108
Cuts at: 7467 7597 8347 8888 9768 10445 11593 11838 1606
Size: 130 750 541 880 677 1148 245 2120
Fragments arranged by size:
2120 1153 1148 880 750 736 719 677
541 433 415 398 390 377 270 246
245 200 174 130 108 84 80 78
NarI GG'CG_CC
Cuts at: 5316 5316
Size: 12352
NcoI C'CATG_G
Cuts at: 513 1065 2151 2406 2788 4857 4911 5523 6771
Size: 552 1086 255 382 2069 54 612 1248
Cuts at: 6771 513
Size: 6094
Fragments arranged by size:
6094 2069 1248 1086 612 552 382 255
54
NdeI CA'TA_TG
Cuts at: 387 6181 6763 8434 387
Size: 5794 582 1671 4305
Fragments arranged by size:
5794 4305 1671 582
NheI G'CTAG_C
Cuts at: 1052 2276 1052
Size: 1224 11128
NotI GC'GGCC_GC
Cuts at: 9799 9799
Size: 12352
NsiI A_TGCA'T
Cuts at: 5256 5737 5256
Size: 481 11871
NspI r_CATG'y
Cuts at: 1132 1229 2504 3469 3676 4273 4486 5239 6448
Size: 97 1275 965 207 597 213 753 1209
Cuts at: 6448 6790 7117 7156 7196 7379 7999 8146 8171
Size: 342 327 39 40 183 620 147 25
Cuts at: 8171 8873 9204 9710 11255 1132
Size: 702 331 506 1545 2229
Fragments arranged by size:
2229 1545 1275 1209 965 753 702 620
597 506 342 331 327 213 207 183
147 97 40 39 25
PasI CC'CwG_GG
Cuts at: 1348 3313 4534 4948 1348
Size: 1965 1221 414 8752
Fragments arranged by size:
8752 1965 1221 414
PciI A'CATG_T
Cuts at: 7113 7995 11251 7113
Size: 882 3256 8214
Fragments arranged by size:
8214 3256 882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3313 3697 5050 8070 1108
Size: 2205 384 1353 3020 5390
Fragments arranged by size:
5390 3020 2205 1353 384
PfoI T'CCnGG_A
Cuts at: 4125 4488 5028 5462 7704 8754 4125
Size: 363 540 434 2242 1050 7723
Fragments arranged by size:
7723 2242 1050 540 434 363
PmeI GTTT'AAAC
Cuts at: 9656 9656
Size: 12352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2949 10421 10453 11934 11966 2917
Size: 32 7472 32 1481 32 3303
Fragments arranged by size:
7472 3303 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 3042 5762 6667 6709 8290 8308 8407 2602
Size: 440 2720 905 42 1581 18 99 6547
Fragments arranged by size:
6547 2720 1581 905 440 99 42 18
PshAI GACnn'nnGTC
Cuts at: 1613 7896 1613
Size: 6283 6069
PsiI TTA'TAA
Cuts at: 9922 9922
Size: 12352
PspOMI G'GGCC_C
Cuts at: 5916 6112 8185 5916
Size: 196 2073 10083
Fragments arranged by size:
10083 2073 196
PspXI vC'TCGA_Gb
Cuts at: 3474 6455 3474
Size: 2981 9371
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8947 8979 8947
Size: 32 12320
PstI C_TGCA'G
Cuts at: 830 2073 3562 5648 6559 6876 7326 7602 9704
Size: 1243 1489 2086 911 317 450 276 2102
Cuts at: 9704 830
Size: 3478
Fragments arranged by size:
3478 2102 2086 1489 1243 911 450 317
276
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12352
PvuII CAG'CTG
Cuts at: 2507 2884 3697 4850 5050 5224 5308 5554 6560
Size: 377 813 1153 200 174 84 246 1006
Cuts at: 6560 7279 7359 8347 8888 2507
Size: 719 80 988 541 5971
Fragments arranged by size:
5971 1153 1006 988 813 719 541 377
246 200 174 84 80
SacI G_AGCT'C
Cuts at: 2136 4575 6015 2136
Size: 2439 1440 8473
Fragments arranged by size:
8473 2439 1440
SalI G'TCGA_C
Cuts at: 1936 9694 1936
Size: 7758 4594
SanDI GG'GwC_CC
Cuts at: 2602 2602
Size: 12352
SapI GCTCTTCn'nnn_
Cuts at: 5069 8612 5069
Size: 3543 8809
SbfI CC_TGCA'GG
Cuts at: 9704 9704
Size: 12352
ScaI AGT'ACT
Cuts at: 2900 7328 10600 12312 2900
Size: 4428 3272 1712 2940
Fragments arranged by size:
4428 3272 2940 1712
SexAI A'CCwGG_T
Cuts at: 4110 4281 6347 4110
Size: 171 2066 10115
Fragments arranged by size:
10115 2066 171
SfcI C'TryA_G
Cuts at: 826 1045 2069 2631 3558 4576 4789 5644 6272
Size: 219 1024 562 927 1018 213 855 628
Cuts at: 6272 6555 6872 7286 7322 7330 7598 7811 8761
Size: 283 317 414 36 8 268 213 950
Cuts at: 8761 9190 9700 10835 11516 11707 826
Size: 429 510 1135 681 191 1471
Fragments arranged by size:
1471 1135 1024 1018 950 927 855 681
628 562 510 429 414 317 283 268
219 213 213 191 36 8
SfoI GGC'GCC
Cuts at: 5317 5317
Size: 12352
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12352
SmaI CCC'GGG
Cuts at: 1872 4733 9679 1872
Size: 2861 4946 4545
Fragments arranged by size:
4946 4545 2861
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3474 4598 6455 6965 10456 11357
Size: 197 926 1531 1124 1857 510 3491 901
Cuts at: 11357 11619 820
Size: 262 1553
Fragments arranged by size:
3491 1857 1553 1531 1124 926 901 510
262 197
SnaBI TAC'GTA
Cuts at: 493 10143 493
Size: 9650 2702
SpeI A'CTAG_T
Cuts at: 152 4796 152
Size: 4644 7708
SphI G_CATG'C
Cuts at: 1132 3469 3676 4273 6448 7196 7379 8171 8873
Size: 2337 207 597 2175 748 183 792 702
Cuts at: 8873 9710 1132
Size: 837 3774
Fragments arranged by size:
3774 2337 2175 837 792 748 702 597
207 183
SspI AAT'ATT
Cuts at: 5 52 10276 5
Size: 47 10224 2081
Fragments arranged by size:
10224 2081 47
StuI AGG'CCT
Cuts at: 1517 3027 4088 5807 5930 6461 7370 7769 8094
Size: 1510 1061 1719 123 531 909 399 325
Cuts at: 8094 8222 9009 9177 1517
Size: 128 787 168 4692
Fragments arranged by size:
4692 1719 1510 1061 909 787 531 399
325 168 128 123
StyI C'CwwG_G
Cuts at: 513 1065 2127 2151 2385 2406 2748 2788 2991
Size: 552 1062 24 234 21 342 40 203
Cuts at: 2991 3022 3678 4857 4911 5523 5725 6462 6678
Size: 31 656 1179 54 612 202 737 216
Cuts at: 6678 6771 7857 9791 513
Size: 93 1086 1934 3074
Fragments arranged by size:
3074 1934 1179 1086 1062 737 656 612
552 342 234 216 203 202 93 54
40 31 24 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 3121 3681 6143 6638 8312 8964
Size: 1255 882 659 560 2462 495 1674 652
Cuts at: 8964 9039 10379 10396 10549 10734 11179 325
Size: 75 1340 17 153 185 445 1498
Fragments arranged by size:
2462 1674 1498 1340 1255 882 659 652
560 495 445 185 153 75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 2968 6816
Size: 274 80 33 51 1443 921 70 3848
Cuts at: 6816 7326 7641 8115 8265 10598 12310 96
Size: 510 315 474 150 2333 1712 138
Fragments arranged by size:
3848 2333 1712 1443 921 510 474 315
274 150 138 80 70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 3888 3920 4271 4303 4530 4562 5046
Size: 32 788 32 351 32 227 32 484
Cuts at: 5046 5078 6776 6808 12312 12344 3068
Size: 32 1698 32 5504 32 3076
Fragments arranged by size:
5504 3076 1698 788 484 351 227 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5434 9242 1316
Size: 4118 3808 4426
Fragments arranged by size:
4426 4118 3808
XbaI T'CTAG_A
Cuts at: 6241 9688 6241
Size: 3447 8905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3532 3914 4144 4516 6712 7858 9037 9629 3532
Size: 382 230 372 2196 1146 1179 592 6255
Fragments arranged by size:
6255 2196 1179 1146 592 382 372 230
XhoI C'TCGA_G
Cuts at: 1943 3474 6455 1943
Size: 1531 2981 7840
Fragments arranged by size:
7840 2981 1531
XmaI C'CCGG_G
Cuts at: 1870 4731 9677 1870
Size: 2861 4946 4545
Fragments arranged by size:
4946 4545 2861
XmnI GAAnn'nnTTC
Cuts at: 9438 10481 9438
Size: 1043 11309
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7868 10160 276
Size: 53 83 186 936 6334 2292 2468
Fragments arranged by size:
6334 2468 2292 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI RsrII SacII SfiI
SrfI SwaI