Restriction map for
pFN21AB0999
(Circular) (Six-base) MAPSORT of: pFN21AB0999.seq Check: 5429 from: 1 to: 12055
pFN21AB0999 12055 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2262 4772 6876 7254 7780 8080 8352 2262
Size: 2510 2104 378 526 300 272 5965
Fragments arranged by size:
5965 2510 2104 526 378 300 272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2115 5739 6788 8666
Size: 53 83 186 936 579 3624 1049 1878
Cuts at: 8666 9865 278
Size: 1199 2468
Fragments arranged by size:
3624 2468 1878 1199 1049 936 579 186
83 53
AccI GT'mk_AC
Cuts at: 1937 6788 7812 9398 1937
Size: 4851 1024 1586 4594
Fragments arranged by size:
4851 4594 1586 1024
Acc65I G'GTAC_C
Cuts at: 9376 9376
Size: 12055
AclI AA'CG_TT
Cuts at: 1563 10182 10555 1563
Size: 8619 373 3063
Fragments arranged by size:
8619 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 2603 2739 4037 4188 4425 4913 4922
Size: 789 182 136 1298 151 237 488 9
Cuts at: 4922 5503 6263 6269 6572 7315 8827 10118 11502
Size: 581 760 6 303 743 1512 1291 1384
Cuts at: 11502 11770 1632
Size: 268 1917
Fragments arranged by size:
1917 1512 1384 1298 1291 789 760 743
581 488 303 268 237 182 151 136
9 6
AfeI AGC'GCT
Cuts at: 2906 2906
Size: 12055
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11858
AflIII A'CryG_T
Cuts at: 1203 3294 10954 11931 1203
Size: 2091 7660 977 1327
Fragments arranged by size:
7660 2091 1327 977
AgeI A'CCGG_T
Cuts at: 2443 10886 2443
Size: 8443 3612
AhdI GACnn_n'nnGTC
Cuts at: 10784 10784
Size: 12055
AleI CACnn'nnGTG
Cuts at: 1166 2035 5675 7487 7694 8445 1166
Size: 869 3640 1812 207 751 4776
Fragments arranged by size:
4776 3640 1812 869 751 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2699 5412 5693 1184
Size: 1515 2713 281 7546
Fragments arranged by size:
7546 2713 1515 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2879 2911 8124 8156 2879
Size: 32 5213 32 6778
Fragments arranged by size:
6778 5213 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3395 4226 5152 6232 6968 8134 9798 11370 3395
Size: 831 926 1080 736 1166 1664 1572 4080
Fragments arranged by size:
4080 1664 1572 1166 1080 926 831 736
ApaI G_GGCC'C
Cuts at: 2754 4305 5468 6239 9273 2754
Size: 1551 1163 771 3034 5536
Fragments arranged by size:
5536 3034 1551 1163 771
ApaLI G'TGCA_C
Cuts at: 4600 6488 9101 10112 11268 4600
Size: 1888 2613 1011 1156 5387
Fragments arranged by size:
5387 2613 1888 1156 1011
ApoI r'AATT_y
Cuts at: 1493 9364 9590 9742 11851 1493
Size: 7871 226 152 2109 1697
Fragments arranged by size:
7871 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10609 160
Size: 872 9577 1606
Fragments arranged by size:
9577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12055
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2282 2627 4729 4857 4959 5061
Size: 73 282 57 345 2102 128 102 102
Cuts at: 5061 5381 5782 6338 6516 6580 6830 7212 7857
Size: 320 401 556 178 64 250 382 645
Cuts at: 7857 7881 9058 9380 1870
Size: 24 1177 322 4545
Fragments arranged by size:
4545 2102 1177 645 556 401 382 345
322 320 282 250 178 128 102 102
73 64 57 24
BamHI G'GATC_C
Cuts at: 2012 6102 9385 9752 2012
Size: 4090 3283 367 4315
Fragments arranged by size:
4315 4090 3283 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 3468 4195 4325 4733
Size: 325 217 627 704 977 727 130 408
Cuts at: 4733 5083 5396 5512 5584 5677 5750 6389 6602
Size: 350 313 116 72 93 73 639 213
Cuts at: 6602 6670 7274 7796 8065 8475 8600 9376 10831
Size: 68 604 522 269 410 125 776 1455
Cuts at: 10831 618
Size: 1842
Fragments arranged by size:
1842 1455 977 776 727 704 639 627
604 522 410 408 350 325 313 269
217 213 130 125 116 93 73 72
68
BanII G_rGCy'C
Cuts at: 1391 2055 2121 2250 2754 3932 4305 4392 4420
Size: 664 66 129 504 1178 373 87 28
Cuts at: 4420 4587 4730 4956 5081 5468 5633 6239 6377
Size: 167 143 226 125 387 165 606 138
Cuts at: 6377 6590 6647 6723 7704 7833 7941 7964 7971
Size: 213 57 76 981 129 108 23 7
Cuts at: 7971 8193 8210 8238 8259 8790 8804 9273 9309
Size: 222 17 28 21 531 14 469 36
Cuts at: 9309 9374 1391
Size: 65 4072
Fragments arranged by size:
4072 1178 981 664 606 531 504 469
387 373 226 222 213 167 165 143
138 129 129 125 108 87 76 66
65 57 36 28 28 23 21 17
14 7
BbeI G_GCGC'C
Cuts at: 3472 4329 4737 6606 8069 3472
Size: 857 408 1869 1463 7458
Fragments arranged by size:
7458 1869 1463 857 408
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 3392 3639 3656 3739 928
Size: 971 510 983 247 17 83 9244
Fragments arranged by size:
9244 983 971 510 247 83 17
BbvCI CC'TCA_GC
Cuts at: 4548 4653 5040 5245 5998 7157 4548
Size: 105 387 205 753 1159 9446
Fragments arranged by size:
9446 1159 753 387 205 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4067 4101 4425 4459 4739 4773 6854
Size: 34 2130 34 324 34 280 34 2081
Cuts at: 6854 6888 10246 10280 1903
Size: 34 3358 34 3678
Fragments arranged by size:
3678 3358 2130 2081 324 280 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9947 11157 9947
Size: 1210 10845
BclI T'GATC_A
Cuts at: 1548 5214 9204 1548
Size: 3666 3990 4399
Fragments arranged by size:
4399 3990 3666
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2191 2430 2500
Size: 107 122 71 631 605 519 239 70
Cuts at: 2500 3075 3815 4655 4670 5064 5600 5982 7791
Size: 575 740 840 15 394 536 382 1809
Cuts at: 7791 7876 10666 136
Size: 85 2790 1525
Fragments arranged by size:
2790 1809 1525 840 740 631 605 575
536 519 394 382 239 122 107 85
71 70 15
BglII A'GATC_T
Cuts at: 2571 3996 8964 12050 2571
Size: 1425 4968 3086 2576
Fragments arranged by size:
4968 3086 2576 1425
BlpI GC'TnA_GC
Cuts at: 8492 9472 8492
Size: 980 11075
Bme1580I G_kGCm'C
Cuts at: 1790 2754 4200 4305 4604 5202 5468 5589 5682
Size: 964 1446 105 299 598 266 121 93
Cuts at: 5682 5753 6176 6239 6392 6492 6523 6673 6831
Size: 71 423 63 153 100 31 150 158
Cuts at: 6831 7263 8605 8736 9105 9273 10116 11272 1790
Size: 432 1342 131 369 168 843 1156 2573
Fragments arranged by size:
2573 1446 1342 1156 964 843 598 432
423 369 299 266 168 158 153 150
131 121 105 100 93 71 63 31
BmgBI CAC'GTC
Cuts at: 1318 2037 5431 5830 5857 7401 7476 1318
Size: 719 3394 399 27 1544 75 5897
Fragments arranged by size:
5897 3394 1544 719 399 75 27
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 4505 5884
Size: 416 53 83 399 24 661 2427 1379
Cuts at: 5884 7647 7799 8208 8562 9786 10744 442
Size: 1763 152 409 354 1224 958 1753
Fragments arranged by size:
2427 1763 1753 1379 1224 958 661 416
409 399 354 152 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 3835 7960 8881 9358 2287
Size: 1548 4125 921 477 4984
Fragments arranged by size:
4984 4125 1548 921 477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2504 2624 3179 3398 4738 7085 7528
Size: 905 360 120 555 219 1340 2347 443
Cuts at: 7528 7625 7955 8294 8372 8631 8796 10715 1239
Size: 97 330 339 78 259 165 1919 2579
Fragments arranged by size:
2579 2347 1919 1340 905 555 443 360
339 330 259 219 165 120 97 78
Bpu10I CC'TnA_GC
Cuts at: 2478 4548 4653 5040 5245 5920 5998 6621 7157
Size: 2070 105 387 205 675 78 623 536
Cuts at: 7157 2478
Size: 7376
Fragments arranged by size:
7376 2070 675 623 536 387 205 105
78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3626 4661 6350 6383 10180 11045 11343 3626
Size: 1035 1689 33 3797 865 298 4338
Fragments arranged by size:
4338 3797 1689 1035 865 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3311 3887 4487 6020 7416 7504 7642
Size: 617 1812 576 600 1533 1396 88 138
Cuts at: 7642 8649 9022 10718 882
Size: 1007 373 1696 2219
Fragments arranged by size:
2219 1812 1696 1533 1396 1007 617 600
576 373 138 88
BsaAI yAC'GTr
Cuts at: 493 1204 6118 9846 493
Size: 711 4914 3728 2702
Fragments arranged by size:
4914 3728 2702 711
BsaBI GATnn'nnATC
Cuts at: 12049 12049
Size: 12055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 3469 4124 4326
Size: 53 83 186 936 579 1357 655 202
Cuts at: 4326 4734 5034 5603 5736 6513 6603 6785 6791
Size: 408 300 569 133 777 90 182 6
Cuts at: 6791 8066 8663 9131 9862 10244 10879 275
Size: 1275 597 468 731 382 635 1451
Fragments arranged by size:
1451 1357 1275 936 777 731 655 635
597 579 569 468 408 382 300 202
186 182 133 90 83 53 6
BsaWI w'CCGG_w
Cuts at: 2443 2706 4363 4412 5569 8710 8922 8987 10488
Size: 263 1657 49 1157 3141 212 65 1501
Cuts at: 10488 10886 11160 11307 11718 2443
Size: 398 274 147 411 2780
Fragments arranged by size:
3141 2780 1657 1501 1157 411 398 274
263 212 147 65 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 2768 2798 2879
Size: 30 165 30 735 30 600 30 81
Cuts at: 2879 2909 4736 4766 5528 5558 5693 5723 6434
Size: 30 1827 30 762 30 135 30 711
Cuts at: 6434 6464 6917 6947 7334 7364 7501 7531 8124
Size: 30 453 30 387 30 137 30 593
Cuts at: 8124 8154 9237 9267 1178
Size: 30 1083 30 3966
Fragments arranged by size:
3966 1827 1083 762 735 711 600 593
453 387 165 137 135 81 30 30
30 30 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2528 2621 2624 2875 3572 3614 3617 3629
Size: 1342 93 3 251 697 42 3 12
Cuts at: 3629 3797 4094 4127 4160 4163 4175 4254 4449
Size: 168 297 33 33 3 12 79 195
Cuts at: 4449 4493 4502 4505 4532 4541 4565 4595 4631
Size: 44 9 3 27 9 24 30 36
Cuts at: 4631 4826 5347 5986 6335 6557 7729 7936 9085
Size: 195 521 639 349 222 1172 207 1149
Cuts at: 9085 9317 1186
Size: 232 3924
Fragments arranged by size:
3924 1342 1172 1149 697 639 521 349
297 251 232 222 207 195 195 168
93 79 44 42 36 33 33 30
27 24 12 12 9 9 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2187 2287 2456 3798 4219 4312 4829 4993 5125
Size: 100 169 1342 421 93 517 164 132
Cuts at: 5125 5372 5561 5666 6625 7140 7279 7323 7703
Size: 247 189 105 959 515 139 44 380
Cuts at: 7703 7918 7952 8436 8789 9029 11258 11754 2187
Size: 215 34 484 353 240 2229 496 2488
Fragments arranged by size:
2488 2229 1342 959 517 515 496 484
421 380 353 247 240 215 189 169
164 139 132 105 100 93 44 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2705 2709 4437 4791 1163
Size: 573 372 597 4 1728 354 8427
Fragments arranged by size:
8427 1728 597 573 372 354 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2363 3099 3898 4665 5803 5943
Size: 275 186 362 736 799 767 1138 140
Cuts at: 5943 6362 6472 8351 9505 10266 11294 1540
Size: 419 110 1879 1154 761 1028 2301
Fragments arranged by size:
2301 1879 1154 1138 1028 799 767 761
736 419 362 275 186 140 110
BsiHKAI G_wGCw'C
Cuts at: 2250 3114 4604 4730 5897 6492 6837 6867 7257
Size: 864 1490 126 1167 595 345 30 390
Cuts at: 7257 7497 8210 8259 9105 10116 10201 11272 2250
Size: 240 713 49 846 1011 85 1071 3033
Fragments arranged by size:
3033 1490 1167 1071 1011 864 846 713
595 390 345 240 126 85 49 30
BsiWI C'GTAC_G
Cuts at: 6445 6445
Size: 12055
BsmI GAATG_Cn'
Cuts at: 4088 4214 5661 9566 9659 4088
Size: 126 1447 3905 93 6484
Fragments arranged by size:
6484 3905 1447 126 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3448 5930 3448
Size: 2482 9573
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2121 2250 2754 3114 3932 4200
Size: 399 265 66 129 504 360 818 268
Cuts at: 4200 4305 4392 4420 4587 4604 4730 4956 5081
Size: 105 87 28 167 17 126 226 125
Cuts at: 5081 5202 5468 5589 5633 5682 5753 5897 6176
Size: 121 266 121 44 49 71 144 279
Cuts at: 6176 6239 6377 6392 6492 6523 6590 6647 6673
Size: 63 138 15 100 31 67 57 26
Cuts at: 6673 6723 6831 6837 6867 7257 7263 7497 7704
Size: 50 108 6 30 390 6 234 207
Cuts at: 7704 7833 7941 7964 7971 8193 8210 8238 8259
Size: 129 108 23 7 222 17 28 21
Cuts at: 8259 8605 8736 8790 8804 9105 9273 9309 9374
Size: 346 131 54 14 301 168 36 65
Cuts at: 9374 10116 10201 11272 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 818 742 504 399 390 360
346 301 279 268 266 265 234 226
222 207 168 167 144 138 131 129
129 126 125 121 121 108 108 105
100 87 85 71 67 66 65 63
57 54 50 49 44 36 31 30
28 28 26 23 21 17 17 15
14 7 6 6
BspEI T'CCGG_A
Cuts at: 11718 11718
Size: 12055
BspHI T'CATG_A
Cuts at: 2803 3288 9944 11674 2803
Size: 485 6656 1730 3184
Fragments arranged by size:
6656 3184 1730 485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2262 2286 4772 5025 6147 6876 6987 7254
Size: 1418 24 2486 253 1122 729 111 267
Cuts at: 7254 7780 8080 8352 9164 9410 844
Size: 526 300 272 812 246 3489
Fragments arranged by size:
3489 2486 1418 1122 812 729 526 300
272 267 253 246 111 24
BsrBI CCG'CTC
Cuts at: 1394 1823 3756 4137 4704 4913 6297 6364 6397
Size: 429 1933 381 567 209 1384 67 33
Cuts at: 6397 7414 7438 7456 7876 7926 9136 9942 1394
Size: 1017 24 18 420 50 1210 806 3507
Fragments arranged by size:
3507 1933 1384 1210 1017 806 567 429
420 381 209 67 50 33 24 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 6591 10550 10724 65
Size: 1183 5343 3959 174 1396
Fragments arranged by size:
5343 3959 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2443 4063 4624 4809 6253 6410 6558
Size: 110 633 1620 561 185 1444 157 148
Cuts at: 6558 6680 7071 7892 8062 9078 10699 10886 1700
Size: 122 391 821 170 1016 1621 187 2869
Fragments arranged by size:
2869 1621 1620 1444 1016 821 633 561
391 187 185 170 157 148 122 110
BsrGI T'GTAC_A
Cuts at: 96 2772 3270 5742 96
Size: 2676 498 2472 6409
Fragments arranged by size:
6409 2676 2472 498
BssHII G'CGCG_C
Cuts at: 1925 6596 1925
Size: 4671 7384
BssSI C'ACGA_G
Cuts at: 3113 6693 6795 7256 10115 11127 3113
Size: 3580 102 461 2859 1012 4041
Fragments arranged by size:
4041 3580 2859 1012 461 102
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3538 5017 6677 6968 7111 794
Size: 2744 1479 1660 291 143 5738
Fragments arranged by size:
5738 2744 1660 1479 291 143
BstBI TT'CG_AA
Cuts at: 10859 10859
Size: 12055
BstEII G'GTnAC_C
Cuts at: 1184 4936 5270 8153 8411 8425 1184
Size: 3752 334 2883 258 14 4814
Fragments arranged by size:
4814 3752 2883 334 258 14
BstXI CCAn_nnnn'nTGG
Cuts at: 5509 7135 7291 7489 8248 8797 5509
Size: 1626 156 198 759 549 8767
Fragments arranged by size:
8767 1626 759 549 198 156
BstYI r'GATC_y
Cuts at: 1972 2012 2396 2571 3021 3996 6102 6309 8964
Size: 40 384 175 450 975 2106 207 2655
Cuts at: 8964 9385 9752 10137 10154 11606 11692 11704 12050
Size: 421 367 385 17 1452 86 12 346
Cuts at: 12050 1972
Size: 1977
Fragments arranged by size:
2655 2106 1977 1452 975 450 421 385
384 367 346 207 175 86 40 17
12
Bsu36I CC'TnA_GG
Cuts at: 3664 7721 3664
Size: 4057 7998
BtgI C'CryG_G
Cuts at: 513 1065 2379 2498 2556 3472 3822 4098 4542
Size: 552 1314 119 58 916 350 276 444
Cuts at: 4542 4659 5475 5493 5549 5704 5719 5797 6082
Size: 117 816 18 56 155 15 78 285
Cuts at: 6082 6276 6356 6635 6664 6943 7039 7134 7290
Size: 194 80 279 29 279 96 95 156
Cuts at: 7290 7713 8158 8596 8611 8871 9258 513
Size: 423 445 438 15 260 387 3310
Fragments arranged by size:
3310 1314 916 816 552 445 444 438
423 387 350 285 279 279 276 260
194 156 155 119 117 96 95 80
78 58 56 29 18 15 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2843 3898 4428 7993 487
Size: 683 56 1617 1055 530 3565 4549
Fragments arranged by size:
4549 3565 1617 1055 683 530 56
BtsI GCAGTG_nn'
Cuts at: 3085 3953 4219 4566 4774 5276 5298 7780 9314
Size: 868 266 347 208 502 22 2482 1534
Cuts at: 9314 9571 10365 10385 3085
Size: 257 794 20 4755
Fragments arranged by size:
4755 2482 1534 868 794 502 347 266
257 208 22 20
ClaI AT'CG_AT
Cuts at: 1554 3132 1554
Size: 1578 10477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12020
DraI TTT'AAA
Cuts at: 9359 9359
Size: 12055
DraIII CAC_nnn'GTG
Cuts at: 3118 8570 3118
Size: 5452 6603
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3284 11062 809
Size: 507 1968 7778 1802
Fragments arranged by size:
7778 1968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2258 2376 3096
Size: 54 1425 50 275 18 428 118 720
Cuts at: 3096 3180 3252 3895 3909 4071 4626 4662 5800
Size: 84 72 643 14 162 555 36 1138
Cuts at: 5800 5940 6273 6359 6469 6507 6560 6682 7125
Size: 140 333 86 110 38 53 122 443
Cuts at: 7125 7868 7894 7996 8348 9080 9502 10391 12020
Size: 743 26 102 352 732 422 889 1629
Cuts at: 12020 8
Size: 43
Fragments arranged by size:
1629 1425 1138 889 743 732 720 643
555 443 428 422 352 333 275 162
140 122 118 110 102 86 84 72
54 53 50 43 38 36 26 18
14
EagI C'GGCC_G
Cuts at: 1537 1812 3096 3895 4662 5800 5940 6359 6469
Size: 275 1284 799 767 1138 140 419 110
Cuts at: 6469 8348 9502 1537
Size: 1879 1154 4090
Fragments arranged by size:
4090 1879 1284 1154 1138 799 767 419
275 140 110
EarI CTCTTCn'nnn_
Cuts at: 1353 3971 6548 8247 9985 1353
Size: 2618 2577 1699 1738 3423
Fragments arranged by size:
3423 2618 2577 1738 1699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2209 3605 5658 6171 8546 8857
Size: 1064 138 620 1396 2053 513 2375 311
Cuts at: 8857 10642 11016 11162 387
Size: 1785 374 146 1280
Fragments arranged by size:
2375 2053 1785 1396 1280 1064 620 513
374 311 146 138
EcoICRI GAG'CTC
Cuts at: 2248 4728 8208 8257 2248
Size: 2480 3480 49 6046
Fragments arranged by size:
6046 3480 2480 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2421 2504 2603 2624 2739 3179
Size: 393 512 277 83 99 21 115 440
Cuts at: 3179 3398 4037 4188 4425 4738 4913 4922 5503
Size: 219 639 151 237 313 175 9 581
Cuts at: 5503 6263 6269 6572 7085 7315 7528 7625 7955
Size: 760 6 303 513 230 213 97 330
Cuts at: 7955 8294 8372 8631 8796 8827 10118 10715 11502
Size: 339 78 259 165 31 1291 597 787
Cuts at: 11502 11770 1239
Size: 268 1524
Fragments arranged by size:
1524 1291 787 760 639 597 581 513
512 440 393 339 330 313 303 277
268 259 237 230 219 213 175 165
151 115 99 97 83 78 31 21
9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 3641 5744 5819 6209 6737 2411
Size: 1230 2103 75 390 528 7729
Fragments arranged by size:
7729 2103 1230 528 390 75
EcoO109I rG'GnC_Cy
Cuts at: 2199 2750 2751 5161 5455 5465 5924 6202 6229
Size: 551 1 2410 294 10 459 278 27
Cuts at: 6229 6236 6265 7162 7208 7852 8303 9162 9270
Size: 7 29 897 46 644 451 859 108
Cuts at: 9270 2199
Size: 4984
Fragments arranged by size:
4984 2410 897 859 644 551 459 451
294 278 108 46 29 27 10 7
1
EcoRI G'AATT_C
Cuts at: 9364 9742 9364
Size: 378 11677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2863 3337 2863
Size: 474 11581
FseI GG_CCGG'CC
Cuts at: 4628 4628
Size: 12055
FspI TGC'GCA
Cuts at: 10561 10561
Size: 12055
HaeII r_GCGC'y
Cuts at: 1678 2326 2908 3472 3810 4064 4329 4508 4676
Size: 648 582 564 338 254 265 179 168
Cuts at: 4676 4737 5694 6606 7064 8069 11202 1678
Size: 61 957 912 458 1005 3133 2531
Fragments arranged by size:
3133 2531 1005 957 912 648 582 564
458 338 265 254 179 168 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2851 2883 3125
Size: 32 135 32 566 32 681 32 242
Cuts at: 3125 3157 3647 3679 4736 4768 5528 5560 6434
Size: 32 490 32 1057 32 760 32 874
Cuts at: 6434 6466 6917 6949 7206 7238 9149 9181 10696
Size: 32 451 32 257 32 1911 32 1515
Cuts at: 10696 10728 10770 10802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1911 1515 1057 874 760 681 566
490 451 257 242 135 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4231 6789 9399 9645 669
Size: 988 281 2293 2558 2610 246 3079
Fragments arranged by size:
3079 2610 2558 2293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 8312 748
Size: 7564 4491
HpaI GTT'AAC
Cuts at: 9645 9645
Size: 12055
KasI G'GCGC_C
Cuts at: 3468 4325 4733 6602 8065 3468
Size: 857 408 1869 1463 7458
Fragments arranged by size:
7458 1869 1463 857 408
KpnI G_GTAC'C
Cuts at: 9380 9380
Size: 12055
MfeI C'AATT_G
Cuts at: 9654 9654
Size: 12055
MluI A'CGCG_T
Cuts at: 11931 11931
Size: 12055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 5361 7397 9369 11169 11353 1305
Size: 1506 2550 2036 1972 1800 184 2007
Fragments arranged by size:
2550 2036 2007 1972 1800 1506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 6275 7998 10
Size: 54 1425 343 546 3897 1723 4067
Fragments arranged by size:
4067 3897 1723 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2035 3236 3266 3293 5675 5882 7133
Size: 648 869 1201 30 27 2382 207 1251
Cuts at: 7133 7289 7487 7593 7673 7694 8445 10013 10372
Size: 156 198 106 80 21 751 1568 359
Cuts at: 10372 10531 518
Size: 159 2042
Fragments arranged by size:
2382 2042 1568 1251 1201 869 751 648
359 207 198 159 156 106 80 30
27 21
MspA1I CmG'CkG
Cuts at: 1606 2191 2602 2770 3073 3178 3474 4544 4652
Size: 585 411 168 303 105 296 1070 108
Cuts at: 4652 4900 5523 5542 5706 5732 5799 6084 6358
Size: 248 623 19 164 26 67 285 274
Cuts at: 6358 6496 6637 6945 6968 7041 7533 7542 7551
Size: 138 141 308 23 73 492 9 9
Cuts at: 7551 7694 8353 8383 8419 8538 8758 9260 9267
Size: 143 659 30 36 119 220 502 7
Cuts at: 9267 9471 10148 11296 11541 1606
Size: 204 677 1148 245 2120
Fragments arranged by size:
2120 1148 1070 677 659 623 585 502
492 411 308 303 296 285 274 248
245 220 204 168 164 143 141 138
119 108 105 73 67 36 30 26
23 19 9 9 7
NaeI GCC'GGC
Cuts at: 4065 4626 6255 6560 6682 8064 4065
Size: 561 1629 305 122 1382 8056
Fragments arranged by size:
8056 1629 1382 561 305 122
NarI GG'CG_CC
Cuts at: 3469 4326 4734 6603 8066 3469
Size: 857 408 1869 1463 7458
Fragments arranged by size:
7458 1869 1463 857 408
NcoI C'CATG_G
Cuts at: 513 1065 2556 3822 5475 5493 7134 7290 8596
Size: 552 1491 1266 1653 18 1641 156 1306
Cuts at: 8596 8871 513
Size: 275 3697
Fragments arranged by size:
3697 1653 1641 1491 1306 1266 552 275
156 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12055
NgoMIV G'CCGG_C
Cuts at: 4063 4624 6253 6558 6680 8062 4063
Size: 561 1629 305 122 1382 8056
Fragments arranged by size:
8056 1629 1382 561 305 122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12055
NotI GC'GGCC_GC
Cuts at: 6359 8348 9502 6359
Size: 1989 1154 8912
Fragments arranged by size:
8912 1989 1154
NspI r_CATG'y
Cuts at: 1132 1229 3055 3298 4181 5234 5450 5881 9413
Size: 97 1826 243 883 1053 216 431 3532
Cuts at: 9413 10958 1132
Size: 1545 2229
Fragments arranged by size:
3532 2229 1826 1545 1053 883 431 243
216 97
PasI CC'CwG_GG
Cuts at: 1348 4321 5284 6232 8399 8528 1348
Size: 2973 963 948 2167 129 4875
Fragments arranged by size:
4875 2973 2167 963 948 129
PciI A'CATG_T
Cuts at: 3294 10954 3294
Size: 7660 4395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 2920 8134 8422 1108
Size: 1422 390 5214 288 4741
Fragments arranged by size:
5214 4741 1422 390 288
PfoI T'CCnGG_A
Cuts at: 2121 3324 3375 3885 8127 8547 8738 2121
Size: 1203 51 510 4242 420 191 5438
Fragments arranged by size:
5438 4242 1203 510 420 191 51
PmeI GTTT'AAAC
Cuts at: 9359 9359
Size: 12055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2323 2879 2911 2996 3028 8124 8156 10124
Size: 32 556 32 85 32 5096 32 1968
Cuts at: 10124 10156 11637 11669 2291
Size: 32 1481 32 2677
Fragments arranged by size:
5096 2677 1968 1481 556 85 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5161 5924 6202 7208 5161
Size: 763 278 1006 10008
Fragments arranged by size:
10008 1006 763 278
PshAI GACnn'nnGTC
Cuts at: 1613 8666 1613
Size: 7053 5002
PsiI TTA'TAA
Cuts at: 9625 9625
Size: 12055
PspOMI G'GGCC_C
Cuts at: 2750 4301 5464 6235 9269 2750
Size: 1551 1163 771 3034 5536
Fragments arranged by size:
5536 3034 1551 1163 771
PstI C_TGCA'G
Cuts at: 830 3045 4769 5022 6738 9219 9407 830
Size: 2215 1724 253 1716 2481 188 3478
Fragments arranged by size:
3478 2481 2215 1724 1716 253 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12055
PvuII CAG'CTG
Cuts at: 2602 3178 4900 5542 5732 8538 2602
Size: 576 1722 642 190 2806 6119
Fragments arranged by size:
6119 2806 1722 642 576 190
SacI G_AGCT'C
Cuts at: 2250 4730 8210 8259 2250
Size: 2480 3480 49 6046
Fragments arranged by size:
6046 3480 2480 49
SacII CC_GC'GG
Cuts at: 3475 4545 5707 5800 6085 6359 6638 6946 7042
Size: 1070 1162 93 285 274 279 308 96
Cuts at: 7042 9261 3475
Size: 2219 6269
Fragments arranged by size:
6269 2219 1162 1070 308 285 279 274
96 93
SalI G'TCGA_C
Cuts at: 1936 6787 9397 1936
Size: 4851 2610 4594
Fragments arranged by size:
4851 4594 2610
SanDI GG'GwC_CC
Cuts at: 5161 7208 5161
Size: 2047 10008
SapI GCTCTTCn'nnn_
Cuts at: 6548 8247 6548
Size: 1699 10356
SbfI CC_TGCA'GG
Cuts at: 6738 9407 6738
Size: 2669 9386
ScaI AGT'ACT
Cuts at: 2999 10303 12015 2999
Size: 7304 1712 3039
Fragments arranged by size:
7304 3039 1712
SexAI A'CCwGG_T
Cuts at: 5266 7513 5266
Size: 2247 9808
SfcI C'TryA_G
Cuts at: 826 1045 3041 4765 5018 6734 8170 9215 9403
Size: 219 1996 1724 253 1716 1436 1045 188
Cuts at: 9403 10538 11219 11410 826
Size: 1135 681 191 1471
Fragments arranged by size:
1996 1724 1716 1471 1436 1135 1045 681
253 219 191 188
SfiI GGCCn_nnn'nGGCC
Cuts at: 4655 7876 4655
Size: 3221 8834
SfoI GGC'GCC
Cuts at: 3470 4327 4735 6604 8067 3470
Size: 857 408 1869 1463 7458
Fragments arranged by size:
7458 1869 1463 857 408
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12055
SgrAI Cr'CCGG_yG
Cuts at: 1700 4063 6410 8062 1700
Size: 2363 2347 1652 5693
Fragments arranged by size:
5693 2363 2347 1652
SmaI CCC'GGG
Cuts at: 1872 2227 4859 4961 5063 6518 7859 9060 9382
Size: 355 2632 102 102 1455 1341 1201 322
Cuts at: 9382 1872
Size: 4545
Fragments arranged by size:
4545 2632 1455 1341 1201 355 322 102
102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3641 4676 5381 6365 6398 10159
Size: 197 926 1698 1035 705 984 33 3761
Cuts at: 10159 11060 11322 820
Size: 901 262 1553
Fragments arranged by size:
3761 1698 1553 1035 984 926 901 705
262 197 33
SnaBI TAC'GTA
Cuts at: 493 9846 493
Size: 9353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12055
SphI G_CATG'C
Cuts at: 1132 3055 5234 5450 9413 1132
Size: 1923 2179 216 3963 3774
Fragments arranged by size:
3963 3774 2179 1923 216
SrfI GCCC'GGGC
Cuts at: 6518 6518
Size: 12055
SspI AAT'ATT
Cuts at: 5 52 9979 5
Size: 47 9927 2081
Fragments arranged by size:
9927 2081 47
StuI AGG'CCT
Cuts at: 1517 2745 2927 5222 6215 1517
Size: 1228 182 2295 993 7357
Fragments arranged by size:
7357 2295 1228 993 182
StyI C'CwwG_G
Cuts at: 513 1065 2412 2508 2556 2976 3822 4281 4473
Size: 552 1347 96 48 420 846 459 192
Cuts at: 4473 4629 4823 5407 5451 5475 5493 5820 5898
Size: 156 194 584 44 24 18 327 78
Cuts at: 5898 7134 7290 7845 8596 8871 9012 9494 513
Size: 1236 156 555 751 275 141 482 3074
Fragments arranged by size:
3074 1347 1236 846 751 584 555 552
482 459 420 327 275 194 192 156
156 141 96 78 48 44 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2559 4366 5289 5341 5407 5732 6099
Size: 1255 979 1807 923 52 66 325 367
Cuts at: 6099 6224 6789 6855 7516 7793 8227 8496 8581
Size: 125 565 66 661 277 434 269 85
Cuts at: 8581 8656 8663 8870 8930 10082 10099 10252 10437
Size: 75 7 207 60 1152 17 153 185
Cuts at: 10437 10882 325
Size: 445 1498
Fragments arranged by size:
1807 1498 1255 1152 979 923 661 565
445 434 367 325 277 269 207 185
153 125 85 75 66 66 60 52
17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 2850 2997
Size: 274 80 33 51 1443 795 78 147
Cuts at: 2997 3270 5742 10301 12013 96
Size: 273 2472 4559 1712 138
Fragments arranged by size:
4559 2472 1712 1443 795 274 273 147
138 80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 5846 5878 6087 6119 7334 7366 9237
Size: 32 3754 32 209 32 1215 32 1871
Cuts at: 9237 9269 12015 12047 2060
Size: 32 2746 32 2068
Fragments arranged by size:
3754 2746 2068 1871 1215 209 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3940 1316
Size: 2624 9431
XbaI T'CTAG_A
Cuts at: 9391 9391
Size: 12055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 3077 3919 5153 5458 7486 8131 2023
Size: 1054 842 1234 305 2028 645 5947
Fragments arranged by size:
5947 2028 1234 1054 842 645 305
XhoI C'TCGA_G
Cuts at: 1943 5381 1943
Size: 3438 8617
XmaI C'CCGG_G
Cuts at: 1870 2225 4857 4959 5061 6516 7857 9058 9380
Size: 355 2632 102 102 1455 1341 1201 322
Cuts at: 9380 1870
Size: 4545
Fragments arranged by size:
4545 2632 1455 1341 1201 355 322 102
102
XmnI GAAnn'nnTTC
Cuts at: 3011 10184 3011
Size: 7173 4882
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2113 5737 6786 8664
Size: 53 83 186 936 579 3624 1049 1878
Cuts at: 8664 9863 276
Size: 1199 2468
Fragments arranged by size:
3624 2468 1878 1199 1049 936 579 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SwaI