Restriction map for
pFN21AB0994
(Circular) (Six-base) MAPSORT of: pFN21AB0994.seq Check: 8403 from: 1 to: 6088
pFN21AB0994 6088 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 2731 2005
Size: 726 5362
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 3431 1937
Size: 913 581 4594
Fragments arranged by size:
4594 913 581
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2704 4151 5535 5803 1632
Size: 1072 1447 1384 268 1917
Fragments arranged by size:
1917 1447 1384 1072 268
AfeI AGC'GCT
Cuts at: 3353 3353
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2043 2209 4987 5964 1203
Size: 840 166 2778 977 1327
Fragments arranged by size:
2778 1327 977 840 166
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2252 2794 3831 5403 2252
Size: 542 1037 1572 2937
Fragments arranged by size:
2937 1572 1037 542
ApaI G_GGCC'C
Cuts at: 2519 3114 3165 2519
Size: 595 51 5442
Fragments arranged by size:
5442 595 51
ApaLI G'TGCA_C
Cuts at: 2525 4145 5301 2525
Size: 1620 1156 3312
Fragments arranged by size:
3312 1620 1156
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2271 3409 4864 618
Size: 325 217 627 484 1138 1455 1842
Fragments arranged by size:
1842 1455 1138 627 484 325 217
BanII G_rGCy'C
Cuts at: 1391 2373 2519 2619 3045 3114 3165 3345 3407
Size: 982 146 100 426 69 51 180 62
Cuts at: 3407 1391
Size: 4072
Fragments arranged by size:
4072 982 426 180 146 100 69 62
51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5117
BbvCI CC'TCA_GC
Cuts at: 3132 3215 3132
Size: 83 6005
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 2469 1548
Size: 921 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3128 4699 136
Size: 107 122 71 631 605 1456 1571 1525
Fragments arranged by size:
1571 1525 1456 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2276 2492 2519 2529 3114 3165 4149 5305
Size: 486 216 27 10 585 51 984 1156
Cuts at: 5305 1790
Size: 2573
Fragments arranged by size:
2573 1156 984 585 486 216 51 27
10
BmgBI CAC'GTC
Cuts at: 1318 2210 2485 1318
Size: 892 275 4921
Fragments arranged by size:
4921 892 275
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2502 2522 3819
Size: 416 53 83 399 24 1085 20 1297
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 1297 1085 958 416 399 83 53
24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2011 2405 2575 2789 3005 3308 3323 3329
Size: 772 394 170 214 216 303 15 6
Cuts at: 3329 3386 4748 1239
Size: 57 1362 2579
Fragments arranged by size:
2579 1362 772 394 303 216 214 170
57 15 6
Bpu10I CC'TnA_GC
Cuts at: 3132 3215 3132
Size: 83 6005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3164 4213 5078 5376 3164
Size: 1049 865 298 3876
Fragments arranged by size:
3876 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 3193 3879 493
Size: 711 840 1149 686 2702
Fragments arranged by size:
2702 1149 840 711 686
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2880 4521 4919 5193 5340 5751 2880
Size: 1641 398 274 147 411 3217
Fragments arranged by size:
3217 1641 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 2909 2939 3077
Size: 30 165 30 1362 30 114 30 138
Cuts at: 3077 3107 1178
Size: 30 4159
Fragments arranged by size:
4159 1362 165 138 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2786 3149 3158 3320 3323 3383 1186
Size: 1600 363 9 162 3 60 3891
Fragments arranged by size:
3891 1600 363 162 60 9 3
BseYI C'CCAG_C
Cuts at: 2949 3142 3158 3278 5291 5787 2949
Size: 193 16 120 2013 496 3250
Fragments arranged by size:
3250 2013 496 193 120 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2299 2472 2700 2900 3008 3356 1163
Size: 573 563 173 228 200 108 348 3895
Fragments arranged by size:
3895 573 563 348 228 200 173 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 3345 4149 4234 5305 2529
Size: 816 804 85 1071 3312
Fragments arranged by size:
3312 1071 816 804 85
BsmI GAATG_Cn'
Cuts at: 2227 3599 3692 2227
Size: 1372 93 4623
Fragments arranged by size:
4623 1372 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2098 2098
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2276 2373 2492 2519 2529 2619 3045
Size: 399 486 97 119 27 10 90 426
Cuts at: 3045 3114 3165 3345 3407 4149 4234 5305 1391
Size: 69 51 180 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 426 399 180 119
97 90 85 69 62 51 27 10
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 2444 2731 3443 844
Size: 1161 439 287 712 3489
Fragments arranged by size:
3489 1161 712 439 287
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 2737 3031 3975 1394
Size: 429 202 712 294 944 3507
Fragments arranged by size:
3507 944 712 429 294 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 3045 4583 4757 65
Size: 1183 1044 753 1538 174 1396
Fragments arranged by size:
1538 1396 1183 1044 753 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 2056 4732 4919 1700
Size: 110 200 46 2676 187 2869
Fragments arranged by size:
2869 2676 200 187 110 46
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2657 3026 4148 5160 2657
Size: 369 1122 1012 3585
Fragments arranged by size:
3585 1122 1012 369
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 2048 2404 2048
Size: 356 5732
BstYI r'GATC_y
Cuts at: 1972 2334 2703 3418 3785 4170 4187 5639 5725
Size: 362 369 715 367 385 17 1452 86
Cuts at: 5725 5737 6083 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 715 385 369 367 362 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2229 2712 3330 513
Size: 552 1164 483 618 3271
Fragments arranged by size:
3271 1164 618 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2248 487
Size: 683 56 1022 4327
Fragments arranged by size:
4327 1022 683 56
BtsI GCAGTG_nn'
Cuts at: 2688 3604 4398 4418 2688
Size: 916 794 20 4358
Fragments arranged by size:
4358 916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2318 5095 809
Size: 507 1002 2777 1802
Fragments arranged by size:
2777 1802 1002 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2239 2499
Size: 54 1425 50 275 18 228 181 260
Cuts at: 2499 3535 4424 6053 8
Size: 1036 889 1629 43
Fragments arranged by size:
1629 1425 1036 889 275 260 228 181
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4018 1353
Size: 2665 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4675 5049 5195 387
Size: 1064 138 438 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 438 374 146 138
EcoICRI GAG'CTC
Cuts at: 3343 3343
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2011 2405 2575 2704 2789 3005 3308
Size: 393 379 394 170 129 85 216 303
Cuts at: 3308 3323 3329 3386 4151 4748 5535 5803 1239
Size: 15 6 57 765 597 787 268 1524
Fragments arranged by size:
1524 787 765 597 394 393 379 303
268 216 170 129 85 57 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3080 3080
Size: 6088
EcoO109I rG'GnC_Cy
Cuts at: 2175 2347 2664 2905 3088 3110 3162 3229 2175
Size: 172 317 241 183 22 52 67 5034
Fragments arranged by size:
5034 317 241 183 172 67 52 22
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 2606 4594 2606
Size: 1988 4100
FspAI rTGC'GCAy
Cuts at: 2606 2606
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2750 3355 5235 1678
Size: 1072 605 1880 2531
Fragments arranged by size:
2531 1880 1072 605
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2765 2797 3046 3078 3278
Size: 32 135 32 1193 32 249 32 200
Cuts at: 3278 3310 4729 4761 4803 4835 1373
Size: 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1419 1193 249 200 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2131 3223 3432 3678 669
Size: 988 281 193 1092 209 246 3079
Fragments arranged by size:
3079 1092 988 281 246 209 193
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 3051 5202 5386 1305
Size: 1368 378 2151 184 2007
Fragments arranged by size:
2151 2007 1368 378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 10
Size: 54 1425 343 409 3857
Fragments arranged by size:
3857 1425 409 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 3050 3056 4046 4405 4564 518
Size: 648 1155 729 6 990 359 159 2042
Fragments arranged by size:
2042 1155 990 729 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2166 2779 2808 2953 3504 4181 5329 5574
Size: 560 613 29 145 551 677 1148 245
Cuts at: 5574 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 613 560 551 245 145
29
NaeI GCC'GGC
Cuts at: 2012 2058 2012
Size: 46 6042
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2010 2056 2010
Size: 46 6042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2971 3446 4991 1132
Size: 97 1742 475 1545 2229
Fragments arranged by size:
2229 1742 1545 475 97
PasI CC'CwG_GG
Cuts at: 1348 2194 1348
Size: 846 5242
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 2044 2044
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2664 3088 2664
Size: 424 5664
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2515 3110 3161 2515
Size: 595 51 5442
Fragments arranged by size:
5442 595 51
PstI C_TGCA'G
Cuts at: 830 2790 3440 830
Size: 1960 650 3478
Fragments arranged by size:
3478 1960 650
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SacI G_AGCT'C
Cuts at: 3345 3345
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SanDI GG'GwC_CC
Cuts at: 2664 2664
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 2790 3440 2790
Size: 650 5438
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2542 2786 3436 4571 5252 5443 826
Size: 219 1497 244 650 1135 681 191 1471
Fragments arranged by size:
1497 1471 1135 681 650 244 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3179 4192 5093 5355 820
Size: 197 926 1236 1013 901 262 1553
Fragments arranged by size:
1553 1236 1013 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3193 3879 493
Size: 2700 686 2702
Fragments arranged by size:
2702 2700 686
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 2571 1517
Size: 1054 5034
StyI C'CwwG_G
Cuts at: 513 1065 2061 2940 3384 3527 513
Size: 552 996 879 444 143 3074
Fragments arranged by size:
3074 996 879 552 444 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2544 3115 4115 4132 4285 4470 4915
Size: 1255 964 571 1000 17 153 185 445
Cuts at: 4915 325
Size: 1498
Fragments arranged by size:
1498 1255 1000 964 571 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2909 2941 6048 6080 2909
Size: 32 3107 32 2917
Fragments arranged by size:
3107 2917 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2288 1316
Size: 972 5116
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2980 2509
Size: 471 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI KasI NarI
NruI NsiI PacI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI