Restriction map for
pFN21AB0987
(Circular) (Six-base) MAPSORT of: pFN21AB0987.seq Check: 3383 from: 1 to: 5479
pFN21AB0987 5479 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 2822 1937
Size: 517 368 4594
Fragments arranged by size:
4594 517 368
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2384 2687 2744 3542 4926 5194 1632
Size: 455 297 303 57 798 1384 268 1917
Fragments arranged by size:
1917 1384 798 455 303 297 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 2301 4378 5355 1203
Size: 1098 2077 977 1327
Fragments arranged by size:
2077 1327 1098 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2167 2199 2167
Size: 32 5447
AlwNI CAG_nnn'CTG
Cuts at: 2147 2247 2440 2539 3222 4794 2147
Size: 100 193 99 683 1572 2832
Fragments arranged by size:
2832 1572 683 193 100 99
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 1870
Size: 73 861 4545
Fragments arranged by size:
4545 861 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2519 2800 4255 618
Size: 325 217 627 732 281 1455 1842
Fragments arranged by size:
1842 1455 732 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2798 1391
Size: 1407 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 928
Size: 971 247 4261
Fragments arranged by size:
4261 971 247
BbvCI CC'TCA_GC
Cuts at: 2140 2336 2529 2140
Size: 196 193 5090
Fragments arranged by size:
5090 196 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4090 136
Size: 107 122 71 631 605 2418 1525
Fragments arranged by size:
2418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2415 2896 2415
Size: 481 4998
Bme1580I G_kGCm'C
Cuts at: 1790 2524 3540 4696 1790
Size: 734 1016 1156 2573
Fragments arranged by size:
2573 1156 1016 734
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2722 3210 4168
Size: 416 53 83 399 24 1305 488 958
Cuts at: 4168 442
Size: 1753
Fragments arranged by size:
1753 1305 958 488 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2495 2570 2575 4139 1239
Size: 1256 75 5 1564 2579
Fragments arranged by size:
2579 1564 1256 75 5
Bpu10I CC'TnA_GC
Cuts at: 2140 2336 2529 2140
Size: 196 193 5090
Fragments arranged by size:
5090 196 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 3604 4469 4767 2393
Size: 1211 865 298 3105
Fragments arranged by size:
3105 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4142 882
Size: 617 2643 2219
Fragments arranged by size:
2643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3286 3668 4303 275
Size: 53 83 186 936 1753 382 635 1451
Fragments arranged by size:
1753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3912 4310 4584 4731 5142 3912
Size: 398 274 147 411 4249
Fragments arranged by size:
4249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5254
Fragments arranged by size:
5254 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2204 2267 1186
Size: 862 156 63 4398
Fragments arranged by size:
4398 862 156 63
BseYI C'CCAG_C
Cuts at: 2480 2515 2539 4682 5178 2480
Size: 35 24 2143 496 2781
Fragments arranged by size:
2781 2143 496 35 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 1163
Size: 573 524 4382
Fragments arranged by size:
4382 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 2556 2701 3540 3625 4696 2084
Size: 472 145 839 85 1071 2867
Fragments arranged by size:
2867 1071 839 472 145 85
BsmI GAATG_Cn'
Cuts at: 2285 2677 2990 3083 2285
Size: 392 313 93 4681
Fragments arranged by size:
4681 392 313 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2524 2556 2701 2798 3540 3625
Size: 399 294 440 32 145 97 742 85
Cuts at: 3625 4696 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 294 145 97
85 32
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 2311 3368 5098 2311
Size: 1057 1730 2692
Fragments arranged by size:
2692 1730 1057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 2834 844
Size: 1388 602 3489
Fragments arranged by size:
3489 1388 602
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 3974 4148 65
Size: 1183 1140 1586 174 1396
Fragments arranged by size:
1586 1396 1183 1140 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2088 2440 794
Size: 1294 352 3833
Fragments arranged by size:
3833 1294 352
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2458 513
Size: 552 1393 3534
Fragments arranged by size:
3534 1393 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2540 2995 3789 3809 2540
Size: 455 794 20 4210
Fragments arranged by size:
4210 794 455 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2640 2926
Size: 54 1425 50 275 18 741 69 286
Cuts at: 2926 3815 5444 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 741 286 275 69 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2225 3409 1353
Size: 752 120 1184 3423
Fragments arranged by size:
3423 1184 752 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2384 2495 2570 2575 2687 2744
Size: 393 455 297 111 75 5 112 57
Cuts at: 2744 3542 4139 4926 5194 1239
Size: 798 597 787 268 1524
Fragments arranged by size:
1524 798 787 597 455 393 297 268
112 111 75 57 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2069
Size: 5479
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 4626 1678
Size: 2948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4120 4152 4194 4226 1373
Size: 32 135 32 2548 32 42 32 2626
Fragments arranged by size:
2626 2548 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2712 4593 4777 1305
Size: 1407 1881 184 2007
Fragments arranged by size:
2007 1881 1407 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2404 2515 2548 2895 3572 4720 4965 1606
Size: 798 111 33 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 347 245 111 33
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4927
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2129 2305 2560 2837 4382 1132
Size: 97 900 176 255 277 1545 2229
Fragments arranged by size:
2229 1545 900 277 255 176 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 2301 4378 2301
Size: 2077 3402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 1108
Size: 1365 4114
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2436 2445 2831 830
Size: 1606 9 386 3478
Fragments arranged by size:
3478 1606 386 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2515 2548 2515
Size: 33 5446
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2432 2441 2827 3962 4643 4834 826
Size: 219 1387 9 386 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 681 386 219 191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 3583 4484 4746 820
Size: 197 926 465 1175 901 262 1553
Fragments arranged by size:
1553 1175 926 901 465 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 2138 1517
Size: 621 4858
StyI C'CwwG_G
Cuts at: 513 1065 2643 2918 513
Size: 552 1578 275 3074
Fragments arranged by size:
3074 1578 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 3506 3523 3676 3861 4306 325
Size: 1255 927 999 17 153 185 445 1498
Fragments arranged by size:
1498 1255 999 927 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2512 2512
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 2754 3608 2754
Size: 854 4625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI