Restriction map for
pFN21AB0986
(Circular) (Six-base) MAPSORT of: pFN21AB0986.seq Check: 5730 from: 1 to: 6067
pFN21AB0986 6067 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2271 3877 278
Size: 53 83 186 936 735 1606 2468
Fragments arranged by size:
2468 1606 936 735 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2162 3410 1937
Size: 225 1248 4594
Fragments arranged by size:
4594 1248 225
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 2507 3092 3116 4130 5514 5782 1632
Size: 800 75 585 24 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1014 800 585 268 75 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 3124 4796 3124
Size: 1672 4395
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 6035
AlwNI CAG_nnn'CTG
Cuts at: 2713 2872 3210 3810 5382 2713
Size: 159 338 600 1572 3398
Fragments arranged by size:
3398 1572 600 338 159
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3376 3602 3754 5863 1493
Size: 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2440 3392 1870
Size: 73 497 952 4545
Fragments arranged by size:
4545 952 497 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 2726 2827 3388 4843
Size: 325 217 627 680 259 101 561 1455
Cuts at: 4843 618
Size: 1842
Fragments arranged by size:
1842 1455 680 627 561 325 259 217
101
BanII G_rGCy'C
Cuts at: 1391 2441 2679 2862 3240 3386 1391
Size: 1050 238 183 378 146 4072
Fragments arranged by size:
4072 1050 378 238 183 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 928
Size: 971 218 4878
Fragments arranged by size:
4878 971 218
BbvCI CC'TCA_GC
Cuts at: 2948 2948
Size: 6067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2225 2259 4258 4292 1903
Size: 34 50 34 204 34 1999 34 3678
Fragments arranged by size:
3678 1999 204 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 2383 2524 1548
Size: 835 141 5091
Fragments arranged by size:
5091 835 141
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2765 4678
Size: 107 122 71 631 605 337 756 1913
Cuts at: 4678 136
Size: 1525
Fragments arranged by size:
1913 1525 756 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2083 3288 6062 2083
Size: 1205 2774 2088
Fragments arranged by size:
2774 2088 1205
BlpI GC'TnA_GC
Cuts at: 2100 2630 3484 2100
Size: 530 854 4683
Fragments arranged by size:
4683 854 530
Bme1580I G_kGCm'C
Cuts at: 1790 4128 5284 1790
Size: 2338 1156 2573
Fragments arranged by size:
2573 2338 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3181 3798 4756
Size: 416 53 83 399 24 1764 617 958
Cuts at: 4756 442
Size: 1753
Fragments arranged by size:
1764 1753 958 617 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2375 2657 2723 3133 3195 3246 4727
Size: 821 315 282 66 410 62 51 1481
Cuts at: 4727 1239
Size: 2579
Fragments arranged by size:
2579 1481 821 410 315 282 66 62
51
Bpu10I CC'TnA_GC
Cuts at: 2948 2948
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 4192 5057 5355 2906
Size: 1286 865 298 3618
Fragments arranged by size:
3618 1286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4730 882
Size: 617 1485 1746 2219
Fragments arranged by size:
2219 1746 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 3874 4256 4891
Size: 53 83 186 936 735 1606 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1606 1451 936 735 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 3244 3274 1178
Size: 30 165 30 996 30 815 30 3971
Fragments arranged by size:
3971 996 815 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2420 2678 3002 3346 1186
Size: 856 378 258 324 344 3907
Fragments arranged by size:
3907 856 378 344 324 258
BseYI C'CCAG_C
Cuts at: 2471 2625 2713 2861 3210 5270 5766 2471
Size: 154 88 148 349 2060 496 2772
Fragments arranged by size:
2772 2060 496 349 154 148 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3026 3161 1163
Size: 573 1290 135 4069
Fragments arranged by size:
4069 1290 573 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2595 2740 4128 4213 5284 2595
Size: 145 1388 85 1071 3378
Fragments arranged by size:
3378 1388 1071 145 85
BsmI GAATG_Cn'
Cuts at: 2369 3578 3671 2369
Size: 1209 93 4765
Fragments arranged by size:
4765 1209 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2933 2933
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2441 2595 2679 2740 2862 3240 3386
Size: 399 651 154 84 61 122 378 146
Cuts at: 3386 4128 4213 5284 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 378 154 146
122 85 84 61
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 3422 844
Size: 2158 420 3489
Fragments arranged by size:
3489 2158 420
BsrBI CCG'CTC
Cuts at: 1394 1823 2641 3954 1394
Size: 429 818 1313 3507
Fragments arranged by size:
3507 1313 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2464 4711 4898 1700
Size: 110 654 2247 187 2869
Fragments arranged by size:
2869 2247 654 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 2678 2984 4127 5139 2678
Size: 306 1143 1012 3606
Fragments arranged by size:
3606 1143 1012 306
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2083 3288 3397 3764 4149 4166 5618 5704
Size: 111 1205 109 367 385 17 1452 86
Cuts at: 5704 5716 6062 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1205 385 367 346 111 109
86 17 12
BstZ17I GTA'TAC
Cuts at: 2163 2163
Size: 6067
BtgI C'CryG_G
Cuts at: 513 1065 2054 513
Size: 552 989 4526
Fragments arranged by size:
4526 989 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3583 4377 4397 2941
Size: 642 794 20 4611
Fragments arranged by size:
4611 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DraIII CAC_nnn'GTG
Cuts at: 2518 3049 2518
Size: 531 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2422 3514 4403
Size: 54 1425 50 275 18 592 1092 889
Cuts at: 4403 6032 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1092 889 592 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2574 2874 3081 3997 1353
Size: 1221 300 207 916 3423
Fragments arranged by size:
3423 1221 916 300 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 2495 4654 5028 5174 387
Size: 1064 138 438 468 2159 374 146 1280
Fragments arranged by size:
2159 1280 1064 468 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2375 2432 2507 2657 2723 3092
Size: 393 428 315 57 75 150 66 369
Cuts at: 3092 3116 3133 3195 3246 4130 4727 5514 5782
Size: 24 17 62 51 884 597 787 268
Cuts at: 5782 1239
Size: 1524
Fragments arranged by size:
1524 884 787 597 428 393 369 315
268 150 75 66 62 57 51 24
17
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2998 2024
Size: 974 5093
EcoO109I rG'GnC_Cy
Cuts at: 2071 3166 2071
Size: 1095 4972
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2234 3307 2234
Size: 1073 4994
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2747 3069 5214 1678
Size: 1069 322 2145 2531
Fragments arranged by size:
2531 2145 1069 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 3110 3142 4708
Size: 32 135 32 827 32 679 32 1566
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 827 679 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3229 3411 3657 669
Size: 988 281 1291 182 246 3079
Fragments arranged by size:
3079 1291 988 281 246 182
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 5181 5365 1305
Size: 813 3063 184 2007
Fragments arranged by size:
3063 2007 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2424 10
Size: 54 1425 343 592 3653
Fragments arranged by size:
3653 1425 592 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4025 4384 4543 518
Size: 648 2859 359 159 2042
Fragments arranged by size:
2859 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2629 2713 3207 3483 4160 5308 5553 1606
Size: 1023 84 494 276 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 677 494 276 245 84
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5515
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2246 2484 3425 4970 1132
Size: 97 1017 238 941 1545 2229
Fragments arranged by size:
2229 1545 1017 941 238 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3210 1108
Size: 2102 3965
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 3166 2071
Size: 1095 4972
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PstI C_TGCA'G
Cuts at: 830 3160 3419 830
Size: 2330 259 3478
Fragments arranged by size:
3478 2330 259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2629 2713 2629
Size: 84 5983
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SexAI A'CCwGG_T
Cuts at: 2019 2019
Size: 6067
SfcI C'TryA_G
Cuts at: 826 1045 3156 3415 4550 5231 5422 826
Size: 219 2111 259 1135 681 191 1471
Fragments arranged by size:
2111 1471 1135 681 259 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 2442 3394 1872
Size: 570 952 4545
Fragments arranged by size:
4545 952 570
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2921 4171 5072 5334 820
Size: 197 926 978 1250 901 262 1553
Fragments arranged by size:
1553 1250 978 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2324 1517
Size: 807 5260
StyI C'CwwG_G
Cuts at: 513 1065 2425 3009 3162 3506 513
Size: 552 1360 584 153 344 3074
Fragments arranged by size:
3074 1360 584 552 344 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2485 4094 4111 4264 4449 4894 325
Size: 1255 905 1609 17 153 185 445 1498
Fragments arranged by size:
1609 1498 1255 905 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3060 4313 6025
Size: 274 80 33 51 1443 1083 1253 1712
Cuts at: 6025 96
Size: 138
Fragments arranged by size:
1712 1443 1253 1083 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 6027 6059 2039
Size: 32 3956 32 2047
Fragments arranged by size:
3956 2047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 2440 3392 1870
Size: 570 952 4545
Fragments arranged by size:
4545 952 570
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2269 3875 276
Size: 53 83 186 936 735 1606 2468
Fragments arranged by size:
2468 1606 936 735 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI Bsu36I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI