Restriction map for
pFN21AB0982
(Circular) (Six-base) MAPSORT of: pFN21AB0982.seq Check: 6618 from: 1 to: 6046
pFN21AB0982 6046 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2577 3070 3389 1937
Size: 640 493 319 4594
Fragments arranged by size:
4594 640 493 319
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2811 2916 3145 4109 5493 5761 1632
Size: 1179 105 229 964 1384 268 1917
Fragments arranged by size:
1917 1384 1179 964 268 229 105
AfeI AGC'GCT
Cuts at: 3098 3098
Size: 6046
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 4945 5922 1203
Size: 3742 977 1327
Fragments arranged by size:
3742 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 3107 1166
Size: 1941 4105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2149 3789 5361 2149
Size: 1640 1572 2834
Fragments arranged by size:
2834 1640 1572
ApaI G_GGCC'C
Cuts at: 2471 2471
Size: 6046
ApaLI G'TGCA_C
Cuts at: 2306 4103 5259 2306
Size: 1797 1156 3093
Fragments arranged by size:
3093 1797 1156
ApoI r'AATT_y
Cuts at: 1493 3355 3581 3733 5842 1493
Size: 1862 226 152 2109 1697
Fragments arranged by size:
2109 1862 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2805 2994 3087 3371 1870
Size: 73 862 189 93 284 4545
Fragments arranged by size:
4545 862 284 189 93 73
BamHI G'GATC_C
Cuts at: 3312 3376 3743 3312
Size: 64 367 5615
Fragments arranged by size:
5615 367 64
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2484 3202 3367 4822 618
Size: 325 217 627 697 718 165 1455 1842
Fragments arranged by size:
1842 1455 718 697 627 325 217 165
BanII G_rGCy'C
Cuts at: 1391 2471 2993 3365 1391
Size: 1080 522 372 4072
Fragments arranged by size:
4072 1080 522 372
BbeI G_GCGC'C
Cuts at: 2488 2488
Size: 6046
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5075
BbvCI CC'TCA_GC
Cuts at: 2695 2956 2695
Size: 261 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 2051 2482
Size: 107 122 71 631 605 370 9 431
Cuts at: 2482 2723 4657 136
Size: 241 1934 1525
Fragments arranged by size:
1934 1525 631 605 431 370 241 122
107 71 9
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2471 3207 4107 5263 1790
Size: 520 161 736 900 1156 2573
Fragments arranged by size:
2573 1156 900 736 520 161
BmgBI CAC'GTC
Cuts at: 1318 2281 1318
Size: 963 5083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2347 2485 2772
Size: 416 53 83 399 24 930 138 287
Cuts at: 2772 2808 2814 3777 4735 442
Size: 36 6 963 958 1753
Fragments arranged by size:
1753 963 958 930 416 399 287 138
83 53 36 24 6
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2515 2865 4706 1239
Size: 1139 137 350 1841 2579
Fragments arranged by size:
2579 1841 1139 350 137
Bpu10I CC'TnA_GC
Cuts at: 2695 2956 2695
Size: 261 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2777 4709 882
Size: 617 1278 1932 2219
Fragments arranged by size:
2219 1932 1278 617
BsaAI yAC'GTr
Cuts at: 493 1204 2253 3014 3837 493
Size: 711 1049 761 823 2702
Fragments arranged by size:
2702 1049 823 761 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2485 3067 3853 4235
Size: 53 83 186 936 952 582 786 382
Cuts at: 4235 4870 275
Size: 635 1451
Fragments arranged by size:
1451 952 936 786 635 582 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3306 4479 4877 5151 5298 5709 3306
Size: 1173 398 274 147 411 3643
Fragments arranged by size:
3643 1173 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2501 2531 2906
Size: 30 165 30 606 30 462 30 375
Cuts at: 2906 2936 1178
Size: 30 4288
Fragments arranged by size:
4288 606 462 375 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 2915 2918 2921 2924 2927 1186
Size: 1603 126 3 3 3 3 4305
Fragments arranged by size:
4305 1603 126 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2227 2472 5249 5745 2227
Size: 245 2777 496 2528
Fragments arranged by size:
2777 2528 496 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2275 1163
Size: 573 315 224 4934
Fragments arranged by size:
4934 573 315 224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2286 2310 3027 3141 4107 4192 5263 2286
Size: 24 717 114 966 85 1071 3069
Fragments arranged by size:
3069 1071 966 717 114 85 24
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2310 2471 2993 3027 3141 3207
Size: 399 496 24 161 522 34 114 66
Cuts at: 3207 3365 4107 4192 5263 1391
Size: 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 496 399 161 158
114 85 66 34 24
BspEI T'CCGG_A
Cuts at: 3306 5709 3306
Size: 2403 3643
BspHI T'CATG_A
Cuts at: 2565 3935 5665 2565
Size: 1370 1730 2946
Fragments arranged by size:
2946 1730 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2589 3401 844
Size: 1745 812 3489
Fragments arranged by size:
3489 1745 812
BsrBI CCG'CTC
Cuts at: 1394 1823 2127 3933 1394
Size: 429 304 1806 3507
Fragments arranged by size:
3507 1806 429 304
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4690 4877 1700
Size: 110 2880 187 2869
Fragments arranged by size:
2880 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 2134 2352 1925
Size: 209 218 5619
Fragments arranged by size:
5619 218 209
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2256 2394 794
Size: 1462 138 4446
Fragments arranged by size:
4446 1462 138
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2823 2326
Size: 497 5549
BstYI r'GATC_y
Cuts at: 1972 2540 3312 3376 3743 4128 4145 5597 5683
Size: 568 772 64 367 385 17 1452 86
Cuts at: 5683 5695 6041 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 772 568 385 367 346 86
64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2677 3208 3326 513
Size: 552 939 673 531 118 3233
Fragments arranged by size:
3233 939 673 552 531 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2404 487
Size: 683 56 1178 4129
Fragments arranged by size:
4129 1178 683 56
BtsI GCAGTG_nn'
Cuts at: 2943 3065 3193 3562 4356 4376 2943
Size: 122 128 369 794 20 4613
Fragments arranged by size:
4613 794 369 128 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DraIII CAC_nnn'GTG
Cuts at: 2256 2412 2256
Size: 156 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2055 2166
Size: 54 1425 50 275 18 213 12 111
Cuts at: 2166 2187 2214 3003 3016 3493 4382 6011 8
Size: 21 27 789 13 477 889 1629 43
Fragments arranged by size:
1629 1425 889 789 477 275 213 111
54 50 43 27 21 18 13 12
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2892 2913 3221 3976 1353
Size: 938 601 21 308 755 3423
Fragments arranged by size:
3423 938 755 601 308 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3284 4633 5007 5153 387
Size: 1064 138 1695 1349 374 146 1280
Fragments arranged by size:
1695 1349 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2515 2811 2865 2916 3145 4109
Size: 393 746 137 296 54 51 229 964
Cuts at: 4109 4706 5493 5761 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 964 787 746 597 393 296 268
229 137 54 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3307 3307
Size: 6046
EcoO109I rG'GnC_Cy
Cuts at: 2017 2467 2468 2621 2639 2802 2017
Size: 450 1 153 18 163 5261
Fragments arranged by size:
5261 450 163 153 18 1
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 2585 4552 2585
Size: 1967 4079
HaeII r_GCGC'y
Cuts at: 1678 2276 2488 3100 5193 1678
Size: 598 212 612 2093 2531
Fragments arranged by size:
2531 2093 612 598 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 4687 4719 4761
Size: 32 135 32 437 32 2646 32 42
Cuts at: 4761 4793 1373
Size: 32 2626
Fragments arranged by size:
2646 2626 437 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2118 2743 3071 3390 3636 669
Size: 988 281 180 625 328 319 246 3079
Fragments arranged by size:
3079 988 625 328 319 281 246 180
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KasI G'GCGC_C
Cuts at: 2484 2484
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 5344 1305
Size: 3855 184 2007
Fragments arranged by size:
3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 2216 3005 3018 10
Size: 54 1425 343 357 27 789 13 3038
Fragments arranged by size:
3038 1425 789 357 343 54 27 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3107 4004 4363 4522 518
Size: 648 1017 924 897 359 159 2042
Fragments arranged by size:
2042 1017 924 897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2974 3082 3462 4139 5287 5532 1606
Size: 436 932 108 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 677 436 380 245 108
NarI GG'CG_CC
Cuts at: 2485 2485
Size: 6046
NcoI C'CATG_G
Cuts at: 513 1065 2004 2677 3208 513
Size: 552 939 673 531 3351
Fragments arranged by size:
3351 939 673 552 531
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 3404 4949 1132
Size: 97 2175 1545 2229
Fragments arranged by size:
2229 2175 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2625 1348
Size: 1277 4769
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PmlI CAC'GTG
Cuts at: 2253 2253
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2017 2621 2802 2017
Size: 604 181 5261
Fragments arranged by size:
5261 604 181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspOMI G'GGCC_C
Cuts at: 2467 2467
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2979 3398 830
Size: 2149 419 3478
Fragments arranged by size:
3478 2149 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 3082 3082
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3069 3388 1936
Size: 1133 319 4594
Fragments arranged by size:
4594 1133 319
SanDI GG'GwC_CC
Cuts at: 2802 2802
Size: 6046
SapI GCTCTTCn'nnn_
Cuts at: 2291 2291
Size: 6046
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2975 3048 3166 3394 4529 5210 5401
Size: 219 1930 73 118 228 1135 681 191
Cuts at: 5401 826
Size: 1471
Fragments arranged by size:
1930 1471 1135 681 228 219 191 118
73
SfiI GGCCn_nnn'nGGCC
Cuts at: 2042 2042
Size: 6046
SfoI GGC'GCC
Cuts at: 2486 2486
Size: 6046
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 2807 2996 3089 3373 1872
Size: 935 189 93 284 4545
Fragments arranged by size:
4545 935 284 189 93
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 2513 1517
Size: 996 5050
StyI C'CwwG_G
Cuts at: 513 1065 2004 2631 2677 3208 3485 513
Size: 552 939 627 46 531 277 3074
Fragments arranged by size:
3074 939 627 552 531 277 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2833 3032 4073 4090 4243 4428 4873
Size: 1255 1253 199 1041 17 153 185 445
Cuts at: 4873 325
Size: 1498
Fragments arranged by size:
1498 1255 1253 1041 445 199 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2361 4292 6004
Size: 274 80 33 51 1443 384 1931 1712
Cuts at: 6004 96
Size: 138
Fragments arranged by size:
1931 1712 1443 384 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 2906 2938 6006 6038 2741
Size: 32 133 32 3068 32 2749
Fragments arranged by size:
3068 2749 133 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 2224
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 2805 2994 3087 3371 1870
Size: 935 189 93 284 4545
Fragments arranged by size:
4545 935 284 189 93
XmnI GAAnn'nnTTC
Cuts at: 2150 3098 4175 2150
Size: 948 1077 4021
Fragments arranged by size:
4021 1077 948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII
SacI SacII SexAI SrfI SwaI