Restriction map for
pFN21AB0980
(Circular) (Six-base) MAPSORT of: pFN21AB0980.seq Check: 8555 from: 1 to: 6055
pFN21AB0980 6055 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 3398 1937
Size: 172 1289 4594
Fragments arranged by size:
4594 1289 172
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2671 4118 5502 5770 1632
Size: 1039 1447 1384 268 1917
Fragments arranged by size:
1917 1447 1384 1039 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2124 1166
Size: 958 5097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2096 1184
Size: 912 5143
AlwNI CAG_nnn'CTG
Cuts at: 3798 5370 3798
Size: 1572 4483
ApaI G_GGCC'C
Cuts at: 3111 3111
Size: 6055
ApaLI G'TGCA_C
Cuts at: 3318 4112 5268 3318
Size: 794 1156 4105
Fragments arranged by size:
4105 1156 794
ApoI r'AATT_y
Cuts at: 1493 3364 3590 3742 5851 1493
Size: 1871 226 152 2109 1697
Fragments arranged by size:
2109 1871 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2388 4609 160
Size: 872 1356 2221 1606
Fragments arranged by size:
2221 1606 1356 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2702 2711 2939 3380 1870
Size: 73 759 9 228 441 4545
Fragments arranged by size:
4545 759 441 228 73 9
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3376 4831 618
Size: 325 217 627 1589 1455 1842
Fragments arranged by size:
1842 1589 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2240 2537 2559 3111 3374 1391
Size: 849 297 22 552 263 4072
Fragments arranged by size:
4072 849 552 297 263 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BbvCI CC'TCA_GC
Cuts at: 2343 2343
Size: 6055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4246 4280 1903
Size: 34 50 34 2225 34 3678
Fragments arranged by size:
3678 2225 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 3947 5157 3116
Size: 831 1210 4014
Fragments arranged by size:
4014 1210 831
BclI T'GATC_A
Cuts at: 1548 2608 1548
Size: 1060 4995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2860 4666 136
Size: 107 122 71 631 605 1188 1806 1525
Fragments arranged by size:
1806 1525 1188 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2024 6050 2024
Size: 4026 2029
BlpI GC'TnA_GC
Cuts at: 2491 2571 3472 2491
Size: 80 901 5074
Fragments arranged by size:
5074 901 80
Bme1580I G_kGCm'C
Cuts at: 1790 2373 2720 3111 3322 4116 5272 1790
Size: 583 347 391 211 794 1156 2573
Fragments arranged by size:
2573 1156 794 583 391 347 211
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2367 2512 2867
Size: 416 53 83 399 24 950 145 355
Cuts at: 2867 3786 4744 442
Size: 919 958 1753
Fragments arranged by size:
1753 958 950 919 416 399 355 145
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2351 2351
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 3253 4715 1239
Size: 1005 1009 1462 2579
Fragments arranged by size:
2579 1462 1009 1005
Bpu10I CC'TnA_GC
Cuts at: 2343 2343
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2322 4718 882
Size: 617 823 2396 2219
Fragments arranged by size:
2396 2219 823 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 3862 4244 4879
Size: 53 83 186 936 816 1513 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1513 1451 936 816 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2400 4488 4886 5160 5307 5718 2400
Size: 2088 398 274 147 411 2737
Fragments arranged by size:
2737 2088 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3074 3157 3226 1186
Size: 1888 83 69 4015
Fragments arranged by size:
4015 1888 83 69
BseYI C'CCAG_C
Cuts at: 2050 2486 2759 5258 5754 2050
Size: 436 273 2499 496 2351
Fragments arranged by size:
2499 2351 496 436 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 1163
Size: 573 696 4786
Fragments arranged by size:
4786 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3322 4116 4201 5272 3322
Size: 794 85 1071 4105
Fragments arranged by size:
4105 1071 794 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2373 2537 2559 2720 3111 3322
Size: 399 450 133 164 22 161 391 211
Cuts at: 3322 3374 4116 4201 5272 1391
Size: 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 391 211 164
161 133 85 52 22
BspEI T'CCGG_A
Cuts at: 2400 5718 2400
Size: 3318 2737
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 3410 844
Size: 1340 1226 3489
Fragments arranged by size:
3489 1340 1226
BsrBI CCG'CTC
Cuts at: 1394 1823 2710 2728 3942 1394
Size: 429 887 18 1214 3507
Fragments arranged by size:
3507 1214 887 429 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3217 4550 4724 65
Size: 1183 1969 1333 174 1396
Fragments arranged by size:
1969 1396 1333 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2331 4699 4886 1700
Size: 110 521 2368 187 2869
Fragments arranged by size:
2869 2368 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2269
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2024 2184 2322 2902 2995 3385 3752 4137
Size: 52 160 138 580 93 390 367 385
Cuts at: 4137 4154 5606 5692 5704 6050 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 580 390 385 367 346 160
138 93 86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2032 2167 2892 3336 513
Size: 552 967 135 725 444 3232
Fragments arranged by size:
3232 967 725 552 444 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2551 3160 487
Size: 683 56 1325 609 3382
Fragments arranged by size:
3382 1325 683 609 56
BtsI GCAGTG_nn'
Cuts at: 3330 3571 4365 4385 3330
Size: 241 794 20 5000
Fragments arranged by size:
5000 794 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3325 3360 529
Size: 35 2761 35 3224
Fragments arranged by size:
3224 2761 35 35
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2253 2448
Size: 54 1425 50 275 18 199 224 195
Cuts at: 2448 3220 3502 4391 6020 8
Size: 772 282 889 1629 43
Fragments arranged by size:
1629 1425 889 772 282 275 224 199
195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3985 1353
Size: 2632 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 2974 4642 5016 5162 387
Size: 1064 138 495 890 1668 374 146 1280
Fragments arranged by size:
1668 1280 1064 890 495 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2671 3253 4118 4715 5502 5770
Size: 393 612 427 582 865 597 787 268
Cuts at: 5770 1239
Size: 1524
Fragments arranged by size:
1524 865 787 612 597 582 427 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2342
Size: 6055
EcoO109I rG'GnC_Cy
Cuts at: 2058 2133 2782 3019 3079 3107 2058
Size: 75 649 237 60 28 5006
Fragments arranged by size:
5006 649 237 75 60 28
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 3259 5202 1678
Size: 1581 1943 2531
Fragments arranged by size:
2531 1943 1581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2894 2926 4696 4728 4770
Size: 32 135 32 1322 32 1770 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1770 1322 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 2871 5169 5353 1305
Size: 1197 369 2298 184 2007
Fragments arranged by size:
2298 2007 1197 369 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2031 2255 2450 3222 10
Size: 54 1425 343 199 224 195 772 2843
Fragments arranged by size:
2843 1425 772 343 224 199 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2124 4013 4372 4531 518
Size: 648 958 1889 359 159 2042
Fragments arranged by size:
2042 1889 958 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2442 2495 2655 2794 3147 3471 4148
Size: 444 392 53 160 139 353 324 677
Cuts at: 4148 5296 5541 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 392 353 324 245
160 139 53
NcoI C'CATG_G
Cuts at: 513 1065 2032 2167 513
Size: 552 967 135 4401
Fragments arranged by size:
4401 967 552 135
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2530 1348
Size: 1182 4873
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PfoI T'CCnGG_A
Cuts at: 2467 2467
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3019 3019
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 3107 3107
Size: 6055
PspXI vC'TCGA_Gb
Cuts at: 2702 2711 2702
Size: 9 6046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 6023
PstI C_TGCA'G
Cuts at: 830 3003 3407 830
Size: 2173 404 3478
Fragments arranged by size:
3478 2173 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2050 2442 2495 2050
Size: 392 53 5610
Fragments arranged by size:
5610 392 53
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SanDI GG'GwC_CC
Cuts at: 3019 3019
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2999 3241 3403 4538 5219 5410 826
Size: 219 1954 242 162 1135 681 191 1471
Fragments arranged by size:
1954 1471 1135 681 242 219 191 162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 2711 4159 5060 5322 820
Size: 197 926 759 9 1448 901 262 1553
Fragments arranged by size:
1553 1448 926 901 759 262 197 9
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 2747 3251 1517
Size: 1230 504 4321
Fragments arranged by size:
4321 1230 504
StyI C'CwwG_G
Cuts at: 513 1065 2032 2167 2188 2247 2262 3494 513
Size: 552 967 135 21 59 15 1232 3074
Fragments arranged by size:
3074 1232 967 552 135 59 21 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4301 6013 96
Size: 274 80 33 51 1443 2324 1712 138
Fragments arranged by size:
2324 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3120 3152 6015 6047 3120
Size: 32 2863 32 3128
Fragments arranged by size:
3128 2863 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2263 2854 3181 2248
Size: 15 591 327 5122
Fragments arranged by size:
5122 591 327 15
XhoI C'TCGA_G
Cuts at: 1943 2702 2711 1943
Size: 759 9 5287
Fragments arranged by size:
5287 759 9
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI