Restriction map for
pFN21AB0974
(Circular) (Six-base) MAPSORT of: pFN21AB0974.seq Check: 3607 from: 1 to: 6016
pFN21AB0974 6016 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2719 2719
Size: 6016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4079 5463 5731 1632
Size: 2447 1384 268 1917
Fragments arranged by size:
2447 1917 1384 268
AfeI AGC'GCT
Cuts at: 3285 3285
Size: 6016
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 2175 4915 5892 1203
Size: 972 2740 977 1327
Fragments arranged by size:
2740 1327 977 972
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 2309 1166
Size: 1143 4873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2942 3222 1184
Size: 1758 280 3978
Fragments arranged by size:
3978 1758 280
AlwNI CAG_nnn'CTG
Cuts at: 2068 3759 5331 2068
Size: 1691 1572 2753
Fragments arranged by size:
2753 1691 1572
ApaI G_GGCC'C
Cuts at: 2282 2368 2592 2282
Size: 86 224 5706
Fragments arranged by size:
5706 224 86
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2818 3325 3551 3703 5812 1493
Size: 1325 507 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 507 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 1870
Size: 73 1398 4545
Fragments arranged by size:
4545 1398 73
BamHI G'GATC_C
Cuts at: 2390 3346 3713 2390
Size: 956 367 4693
Fragments arranged by size:
4693 956 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2400 3044 3337 4792
Size: 325 217 627 379 234 644 293 1455
Cuts at: 4792 618
Size: 1842
Fragments arranged by size:
1842 1455 644 627 379 325 293 234
217
BanII G_rGCy'C
Cuts at: 1391 2256 2282 2368 2592 2815 3335 1391
Size: 865 26 86 224 223 520 4072
Fragments arranged by size:
4072 865 520 224 223 86 26
BbeI G_GCGC'C
Cuts at: 3048 3048
Size: 6016
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2834 928
Size: 971 935 4110
Fragments arranged by size:
4110 971 935
BbvCI CC'TCA_GC
Cuts at: 3234 3234
Size: 6016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2323 2357 3015 3049 4207 4241 1903
Size: 34 386 34 658 34 1158 34 3678
Fragments arranged by size:
3678 1158 658 386 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BfrBI ATG'CAT
Cuts at: 2541 2541
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3042 4627 136
Size: 107 122 71 631 605 1370 1585 1525
Fragments arranged by size:
1585 1525 1370 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2451 6011 2451
Size: 3560 2456
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2282 2368 2405 2592 4077 5233 1790
Size: 492 86 37 187 1485 1156 2573
Fragments arranged by size:
2573 1485 1156 492 187 86 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 3747 4705
Size: 416 53 83 399 24 982 1348 958
Cuts at: 4705 442
Size: 1753
Fragments arranged by size:
1753 1348 982 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 3263 4676 1239
Size: 1214 810 1413 2579
Fragments arranged by size:
2579 1413 1214 810
Bpu10I CC'TnA_GC
Cuts at: 2498 3234 2498
Size: 736 5280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 2661 2931 4141 5006 5304 2416
Size: 245 270 1210 865 298 3128
Fragments arranged by size:
3128 1210 865 298 270 245
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2961 4679 882
Size: 617 1462 1718 2219
Fragments arranged by size:
2219 1718 1462 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3045 3823 4205 4840
Size: 53 83 186 936 1512 778 382 635
Cuts at: 4840 275
Size: 1451
Fragments arranged by size:
1512 1451 936 778 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2271 4449 4847 5121 5268 5679 2271
Size: 2178 398 274 147 411 2608
Fragments arranged by size:
2608 2178 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2870 2900 1178
Size: 30 165 30 1467 30 4294
Fragments arranged by size:
4294 1467 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2520 2878 2934 1186
Size: 1294 40 358 56 4268
Fragments arranged by size:
4268 1294 358 56 40
BseYI C'CCAG_C
Cuts at: 2377 2547 3280 5219 5715 2377
Size: 170 733 1939 496 2678
Fragments arranged by size:
2678 1939 733 496 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2215 3011 3218 1163
Size: 573 345 134 796 207 3961
Fragments arranged by size:
3961 796 573 345 207 134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 2271 4077 4162 5233 2220
Size: 51 1806 85 1071 3003
Fragments arranged by size:
3003 1806 1071 85 51
BsmI GAATG_Cn'
Cuts at: 3108 3527 3620 3108
Size: 419 93 5504
Fragments arranged by size:
5504 419 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2735 2735
Size: 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2256 2271 2282 2368 2405 2592
Size: 399 430 36 15 11 86 37 187
Cuts at: 2592 2815 3335 4077 4162 5233 1391
Size: 223 520 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 430 399 223 187
86 85 37 36 15 11
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2571 2719 3041 3371 844
Size: 1280 447 148 322 330 3489
Fragments arranged by size:
3489 1280 447 330 322 148
BsrBI CCG'CTC
Cuts at: 1394 1823 2196 3903 1394
Size: 429 373 1707 3507
Fragments arranged by size:
3507 1707 429 373
BsrDI GCAATG_nn'
Cuts at: 65 1248 2132 4511 4685 65
Size: 1183 884 2379 174 1396
Fragments arranged by size:
2379 1396 1183 884 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2970 4660 4847 1700
Size: 110 1160 1690 187 2869
Fragments arranged by size:
2869 1690 1160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2068 794
Size: 1274 4742
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 3288 3288
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2390 2451 3168 3346 3713 4098 4115 5567
Size: 418 61 717 178 367 385 17 1452
Cuts at: 5567 5653 5665 6011 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 717 418 385 367 346 178
86 61 17 12
Bsu36I CC'TnA_GG
Cuts at: 2492 2492
Size: 6016
BtgI C'CryG_G
Cuts at: 513 1065 2592 513
Size: 552 1527 3937
Fragments arranged by size:
3937 1527 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 2981 3532 4326 4346 2048
Size: 933 551 794 20 3718
Fragments arranged by size:
3718 933 794 551 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2763 2798 529
Size: 35 2199 35 3747
Fragments arranged by size:
3747 2199 35 35
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3463 4352 5981
Size: 54 1425 50 275 18 1633 889 1629
Cuts at: 5981 8
Size: 43
Fragments arranged by size:
1633 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 3946 1353
Size: 1089 1504 3423
Fragments arranged by size:
3423 1504 1089
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 3263 4079 4676 5463 5731 1239
Size: 393 821 810 816 597 787 268 1524
Fragments arranged by size:
1524 821 816 810 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2961 3037 2961
Size: 76 5940
EcoO109I rG'GnC_Cy
Cuts at: 2279 2365 3238 2279
Size: 86 873 5057
Fragments arranged by size:
5057 873 86
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2069 3048 3287 5163 1678
Size: 391 979 239 1876 2531
Fragments arranged by size:
2531 1876 979 391 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2496 2528 2623
Size: 32 135 32 803 32 89 32 95
Cuts at: 2623 2655 4657 4689 4731 4763 1373
Size: 32 2002 32 42 32 2626
Fragments arranged by size:
2626 2002 803 135 95 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 3360 3606 669
Size: 988 281 409 1013 246 3079
Fragments arranged by size:
3079 1013 988 409 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 2347 3606 2347
Size: 1259 4757
KasI G'GCGC_C
Cuts at: 3044 3044
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 5130 5314 1305
Size: 831 2994 184 2007
Fragments arranged by size:
2994 2007 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2309 2478 2654 3057 3101 3974
Size: 648 1014 129 169 176 403 44 873
Cuts at: 3974 4333 4492 518
Size: 359 159 2042
Fragments arranged by size:
2042 1014 873 648 403 359 176 169
159 129 44
MspA1I CmG'CkG
Cuts at: 1606 2148 2157 3432 4109 5257 5502 1606
Size: 542 9 1275 677 1148 245 2120
Fragments arranged by size:
2120 1275 1148 677 542 245 9
NarI GG'CG_CC
Cuts at: 3045 3045
Size: 6016
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5464
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NsiI A_TGCA'T
Cuts at: 2543 2543
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2099 2545 3374 4919 1132
Size: 97 870 446 829 1545 2229
Fragments arranged by size:
2229 1545 870 829 446 97
PasI CC'CwG_GG
Cuts at: 1348 3039 1348
Size: 1691 4325
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2691 1108
Size: 1583 4433
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PspOMI G'GGCC_C
Cuts at: 2278 2364 2588 2278
Size: 86 224 5706
Fragments arranged by size:
5706 224 86
PstI C_TGCA'G
Cuts at: 830 2801 3368 830
Size: 1971 567 3478
Fragments arranged by size:
3478 1971 567
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2148 2148
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2797 3364 4499 5180 5371 826
Size: 219 1752 567 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 567 219 191
SfoI GGC'GCC
Cuts at: 3046 3046
Size: 6016
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2395 2676 2946 4120 5021 5283
Size: 197 926 452 281 270 1174 901 262
Cuts at: 5283 820
Size: 1553
Fragments arranged by size:
1553 1174 926 901 452 281 270 262
197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 2545 3374 1132
Size: 1413 829 3774
Fragments arranged by size:
3774 1413 829
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6016
StyI C'CwwG_G
Cuts at: 513 1065 2223 2241 2460 2998 3455 513
Size: 552 1158 18 219 538 457 3074
Fragments arranged by size:
3074 1158 552 538 457 219 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4043 4060 4213 4398 4843 325
Size: 1255 2463 17 153 185 445 1498
Fragments arranged by size:
2463 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4262 5974 96
Size: 274 80 33 51 1443 2285 1712 138
Fragments arranged by size:
2285 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 2870 2902 5976 6008 2190
Size: 32 648 32 3074 32 2198
Fragments arranged by size:
3074 2198 648 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3288 3288
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 3007 4145 3007
Size: 1138 4878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI