Restriction map for
pFN21AB0972
(Circular) (Six-base) MAPSORT of: pFN21AB0972.seq Check: 4420 from: 1 to: 6016
pFN21AB0972 6016 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2591 2859 3166 2591
Size: 268 307 5441
Fragments arranged by size:
5441 307 268
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3012 4079 5463 5731 1632
Size: 1380 1067 1384 268 1917
Fragments arranged by size:
1917 1384 1380 1067 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 2025 4915 5892 1203
Size: 822 2890 977 1327
Fragments arranged by size:
2890 1327 977 822
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 2959 3187 4745 2959
Size: 228 1558 4230
Fragments arranged by size:
4230 1558 228
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2340 2812 3049 3238 3759 5331 2340
Size: 472 237 189 521 1572 3025
Fragments arranged by size:
3025 1572 521 472 237 189
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2147 2159 2415 2879 3325 3551 3703 5812
Size: 654 12 256 464 446 226 152 2109
Cuts at: 5812 1493
Size: 1697
Fragments arranged by size:
2109 1697 654 464 446 256 226 152
12
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 1870
Size: 73 1398 4545
Fragments arranged by size:
4545 1398 73
BamHI G'GATC_C
Cuts at: 3211 3346 3713 3211
Size: 135 367 5514
Fragments arranged by size:
5514 367 135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2086 2826 3335 1391
Size: 695 740 509 4072
Fragments arranged by size:
4072 740 695 509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2450 3124 928
Size: 971 551 674 3820
Fragments arranged by size:
3820 971 674 551
BbvCI CC'TCA_GC
Cuts at: 3059 3059
Size: 6016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2040 3908 5118 2040
Size: 1868 1210 2938
Fragments arranged by size:
2938 1868 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2054 4627
Size: 107 122 71 631 605 337 45 2573
Cuts at: 4627 136
Size: 1525
Fragments arranged by size:
2573 1525 631 605 337 122 107 71
45
BglII A'GATC_T
Cuts at: 3306 6011 3306
Size: 2705 3311
BlpI GC'TnA_GC
Cuts at: 2742 2820 3433 2742
Size: 78 613 5325
Fragments arranged by size:
5325 613 78
Bme1580I G_kGCm'C
Cuts at: 1790 2328 4077 5233 1790
Size: 538 1749 1156 2573
Fragments arranged by size:
2573 1749 1156 538
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 2641 3747
Size: 416 53 83 399 24 1176 48 1106
Cuts at: 3747 4705 442
Size: 958 1753
Fragments arranged by size:
1753 1176 1106 958 416 399 83 53
48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2253 3141 4676 1239
Size: 823 191 888 1535 2579
Fragments arranged by size:
2579 1535 888 823 191
Bpu10I CC'TnA_GC
Cuts at: 2085 3059 2085
Size: 974 5042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4141 5006 5304 4141
Size: 865 298 4853
Fragments arranged by size:
4853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 3221 4679 882
Size: 617 1167 555 1458 2219
Fragments arranged by size:
2219 1458 1167 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 2028 3807 493
Size: 711 824 1779 2702
Fragments arranged by size:
2702 1779 824 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3114 3144 1178
Size: 30 165 30 1711 30 4050
Fragments arranged by size:
4050 1711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2768 2798 2801 3239 1186
Size: 1078 504 30 3 438 3963
Fragments arranged by size:
3963 1078 504 438 30 3
BseYI C'CCAG_C
Cuts at: 2012 2179 2329 5219 5715 2012
Size: 167 150 2890 496 2313
Fragments arranged by size:
2890 2313 496 167 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 2485 1163
Size: 573 576 173 4694
Fragments arranged by size:
4694 576 573 173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2943 4077 4162 5233 2100
Size: 843 1134 85 1071 2883
Fragments arranged by size:
2883 1134 1071 843 85
BsmI GAATG_Cn'
Cuts at: 2398 3073 3527 3620 2398
Size: 675 454 93 4794
Fragments arranged by size:
4794 675 454 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2100 2328 2826 2943 3335 4077
Size: 399 296 14 228 498 117 392 742
Cuts at: 4077 4162 5233 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 392 296 228
117 85 14
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3012 3905 5635 3012
Size: 893 1730 3393
Fragments arranged by size:
3393 1730 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2515 2591 2859 3166 3371 844
Size: 1671 76 268 307 205 3489
Fragments arranged by size:
3489 1671 307 268 205 76
BsrBI CCG'CTC
Cuts at: 1394 1823 2746 3903 1394
Size: 429 923 1157 3507
Fragments arranged by size:
3507 1157 923 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2226 2251 4511 4685 65
Size: 1183 978 25 2260 174 1396
Fragments arranged by size:
2260 1396 1183 978 174 25
BsrFI r'CCGG_y
Cuts at: 1700 1810 2168 4660 4847 1700
Size: 110 358 2492 187 2869
Fragments arranged by size:
2869 2492 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 2022 96
Size: 1926 4090
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2934 2934
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 3211 3306 3346 3713 4098 4115 5567 5653
Size: 1239 95 40 367 385 17 1452 86
Cuts at: 5653 5665 6011 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1239 385 367 346 95 86
40 17 12
Bsu36I CC'TnA_GG
Cuts at: 2781 2781
Size: 6016
BtgI C'CryG_G
Cuts at: 513 1065 2004 2696 513
Size: 552 939 692 3833
Fragments arranged by size:
3833 939 692 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2047 487
Size: 683 56 821 4456
Fragments arranged by size:
4456 821 683 56
BtsI GCAGTG_nn'
Cuts at: 2300 2921 3532 4326 4346 2300
Size: 621 611 794 20 3970
Fragments arranged by size:
3970 794 621 611 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 3463 4352
Size: 54 1425 50 275 18 295 1338 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1338 889 295 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2715 3946 1353
Size: 1362 1231 3423
Fragments arranged by size:
3423 1362 1231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2534 3217 4603 4977 5123 387
Size: 1064 138 945 683 1386 374 146 1280
Fragments arranged by size:
1386 1280 1064 945 683 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2253 3012 3141 4079 4676 5463
Size: 393 430 191 759 129 938 597 787
Cuts at: 5463 5731 1239
Size: 268 1524
Fragments arranged by size:
1524 938 787 759 597 430 393 268
191 129
EcoO109I rG'GnC_Cy
Cuts at: 2016 2046 2176 2836 3267 2016
Size: 30 130 660 431 4765
Fragments arranged by size:
4765 660 431 130 30
EcoRI G'AATT_C
Cuts at: 2415 3325 3703 2415
Size: 910 378 4728
Fragments arranged by size:
4728 910 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2881 3088 2857
Size: 24 207 5785
Fragments arranged by size:
5785 207 24
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2813 5163 1678
Size: 1135 2350 2531
Fragments arranged by size:
2531 2350 1135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 2945 2977 3007
Size: 32 135 32 1325 32 16 32 30
Cuts at: 3007 3039 3114 3146 3173 3205 4657 4689 4731
Size: 32 75 32 27 32 1452 32 42
Cuts at: 4731 4763 1373
Size: 32 2626
Fragments arranged by size:
2626 1452 1325 135 75 42 32 32
32 32 32 32 32 32 32 30
27 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 5130 5314 1305
Size: 1548 2277 184 2007
Fragments arranged by size:
2277 2007 1548 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2127 10
Size: 54 1425 343 295 3899
Fragments arranged by size:
3899 1425 343 295 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3196 3974 4333 4492 518
Size: 648 1869 161 778 359 159 2042
Fragments arranged by size:
2042 1869 778 648 359 161 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2571 3160 3432 4109 5257 5502 1606
Size: 473 492 589 272 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 492 473 272 245
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 6016
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4525
Fragments arranged by size:
4525 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 3281 3374 4919 1132
Size: 97 2052 93 1545 2229
Fragments arranged by size:
2229 2052 1545 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2340 2571 3049 3238 3280 1108
Size: 1232 231 478 189 42 3844
Fragments arranged by size:
3844 1232 478 231 189 42
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2176 2836 2016
Size: 160 660 5196
Fragments arranged by size:
5196 660 160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PstI C_TGCA'G
Cuts at: 830 3368 830
Size: 2538 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SanDI GG'GwC_CC
Cuts at: 2016 2016
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 2266 2894 3251 4264 5976 2266
Size: 628 357 1013 1712 2306
Fragments arranged by size:
2306 1712 1013 628 357
SfcI C'TryA_G
Cuts at: 826 1045 3364 4499 5180 5371 826
Size: 219 2319 1135 681 191 1471
Fragments arranged by size:
2319 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4120 5021 5283 820
Size: 197 926 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2552 1517
Size: 1035 4981
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 2826 2832 3455 513
Size: 552 939 351 471 6 623 3074
Fragments arranged by size:
3074 939 623 552 471 351 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 2332 4043 4060 4213 4398 4843
Size: 1255 707 45 1711 17 153 185 445
Cuts at: 4843 325
Size: 1498
Fragments arranged by size:
1711 1498 1255 707 445 185 153 45
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 2073 2264
Size: 274 80 33 51 1443 45 51 191
Cuts at: 2264 2892 3249 4262 5974 96
Size: 628 357 1013 1712 138
Fragments arranged by size:
1712 1443 1013 628 357 274 191 138
80 51 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 2193 2225 5976 6008 2100
Size: 32 61 32 3751 32 2108
Fragments arranged by size:
3751 2108 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 2530 3352 2530
Size: 822 5194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2337 2120
Size: 217 5799
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 3071 4145 3071
Size: 1074 4942
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI