Restriction map for
pFN21AB0968
(Circular) (Six-base) MAPSORT of: pFN21AB0968.seq Check: 7994 from: 1 to: 5998
pFN21AB0968 5998 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2242 3077 2242
Size: 835 5163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2379 3341 1937
Size: 442 962 4594
Fragments arranged by size:
4594 962 442
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2957 4061 5445 5713 1632
Size: 1325 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1325 1104 268
AfeI AGC'GCT
Cuts at: 3089 3089
Size: 5998
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 5966
AlwNI CAG_nnn'CTG
Cuts at: 2861 3741 5313 2861
Size: 880 1572 3546
Fragments arranged by size:
3546 1572 880
ApaI G_GGCC'C
Cuts at: 2310 2664 2310
Size: 354 5644
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2081 3307 3533 3685 5794 1493
Size: 588 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3051 3145 3323 1870
Size: 73 1108 94 178 4545
Fragments arranged by size:
4545 1108 178 94 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 3319 4774 618
Size: 325 217 627 528 1004 1455 1842
Fragments arranged by size:
1842 1455 1004 627 528 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 2310 2664 3317 1391
Size: 835 84 354 653 4072
Fragments arranged by size:
4072 835 653 354 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2347 4609 136
Size: 107 122 71 631 605 675 2262 1525
Fragments arranged by size:
2262 1525 675 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 2565 3415 2565
Size: 850 5148
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2343 2352 2664 4059 5215 1790
Size: 520 33 9 312 1395 1156 2573
Fragments arranged by size:
2573 1395 1156 520 312 33 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2367 2775 3153
Size: 416 53 83 399 24 950 408 378
Cuts at: 3153 3729 4687 442
Size: 576 958 1753
Fragments arranged by size:
1753 958 950 576 416 408 399 378
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3027 3027
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 2232 2514 3047 3282 3311 4658 1239
Size: 790 203 282 533 235 29 1347 2579
Fragments arranged by size:
2579 1347 790 533 282 235 203 29
Bpu10I CC'TnA_GC
Cuts at: 3110 3273 3110
Size: 163 5835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 4123 4988 5286 2042
Size: 2081 865 298 2754
Fragments arranged by size:
2754 2081 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 2193 5992 2193
Size: 3799 2199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3218 3805 4187
Size: 53 83 186 936 476 1209 587 382
Cuts at: 4187 4822 275
Size: 635 1451
Fragments arranged by size:
1451 1209 936 635 587 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2455 2485 3047
Size: 30 165 30 624 30 398 30 562
Cuts at: 3047 3077 1178
Size: 30 4099
Fragments arranged by size:
4099 624 562 398 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2797 3218 1186
Size: 853 758 421 3966
Fragments arranged by size:
3966 853 758 421
BseYI C'CCAG_C
Cuts at: 2583 2635 2664 2845 2861 3175 5201 5697 2583
Size: 52 29 181 16 314 2026 496 2884
Fragments arranged by size:
2884 2026 496 314 181 52 29 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2095 1163
Size: 573 359 5066
Fragments arranged by size:
5066 573 359
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4059 4144 5215 4059
Size: 85 1071 4842
Fragments arranged by size:
4842 1071 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2310 2343 2352 2664 3317 4059
Size: 399 436 84 33 9 312 653 742
Cuts at: 4059 4144 5215 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 436 399 312 85
84 33 9
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 3077 3353 844
Size: 1398 835 276 3489
Fragments arranged by size:
3489 1398 835 276
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 3885 1394
Size: 429 1019 1043 3507
Fragments arranged by size:
3507 1043 1019 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2674 794
Size: 1880 4118
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2182 2327 2531 2704 2900 3175 2053
Size: 129 145 204 173 196 275 4876
Fragments arranged by size:
4876 275 204 196 173 145 129
BstYI r'GATC_y
Cuts at: 1972 3328 3695 4080 4097 5549 5635 5647 5993
Size: 1356 367 385 17 1452 86 12 346
Cuts at: 5993 1972
Size: 1977
Fragments arranged by size:
1977 1452 1356 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2260 2260
Size: 5998
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2474 2750 3130 513
Size: 552 939 177 293 276 380 3381
Fragments arranged by size:
3381 939 552 380 293 276 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 2546 2709 2797 3514 4308 4328 2164
Size: 382 163 88 717 794 20 3834
Fragments arranged by size:
3834 794 717 382 163 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2068 2103 529
Size: 35 1504 35 4424
Fragments arranged by size:
4424 1504 35 35
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DraIII CAC_nnn'GTG
Cuts at: 2756 2756
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3445 4334
Size: 54 1425 50 275 18 225 1390 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1390 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2362 2996 3195 3928 1353
Size: 1009 634 199 733 3423
Fragments arranged by size:
3423 1009 733 634 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2232 2514 2957 3047 3282 3311
Size: 393 397 203 282 443 90 235 29
Cuts at: 3311 4061 4658 5445 5713 1239
Size: 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 597 443 397 393 282
268 235 203 90 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2021 2021
Size: 5998
EcoO109I rG'GnC_Cy
Cuts at: 2323 2660 2661 2970 3149 2323
Size: 337 1 309 179 5172
Fragments arranged by size:
5172 337 309 179 1
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 5998
FspI TGC'GCA
Cuts at: 3041 4504 3041
Size: 1463 4535
FspAI rTGC'GCAy
Cuts at: 3041 3041
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 2806 3091 5145 1678
Size: 1128 285 2054 2531
Fragments arranged by size:
2531 2054 1128 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4639 4671 4713 4745 1373
Size: 32 135 32 3067 32 42 32 2626
Fragments arranged by size:
3067 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5112 5296 1305
Size: 3807 184 2007
Fragments arranged by size:
3807 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 3951
Fragments arranged by size:
3951 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 3956 4315 4474 518
Size: 648 1240 1550 359 159 2042
Fragments arranged by size:
2042 1550 1240 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2587 2858 2889 3414 4091 5239 5484 1606
Size: 981 271 31 525 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 677 525 271 245 31
NcoI C'CATG_G
Cuts at: 513 1065 2004 2181 2474 513
Size: 552 939 177 293 4037
Fragments arranged by size:
4037 939 552 293 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2672 3356 4901 1132
Size: 97 1443 684 1545 2229
Fragments arranged by size:
2229 1545 1443 684 97
PasI CC'CwG_GG
Cuts at: 1348 2303 2327 1348
Size: 955 24 5019
Fragments arranged by size:
5019 955 24
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 4067 4099 5580 5612 3047
Size: 32 988 32 1481 32 3433
Fragments arranged by size:
3433 1481 988 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 3149 2323
Size: 826 5172
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspOMI G'GGCC_C
Cuts at: 2306 2660 2306
Size: 354 5644
PstI C_TGCA'G
Cuts at: 830 2239 3350 830
Size: 1409 1111 3478
Fragments arranged by size:
3478 1409 1111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2587 2889 2587
Size: 302 5696
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SanDI GG'GwC_CC
Cuts at: 3149 3149
Size: 5998
SapI GCTCTTCn'nnn_
Cuts at: 2362 2996 2362
Size: 634 5364
SbfI CC_TGCA'GG
Cuts at: 2239 3350 2239
Size: 1111 4887
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2235 3346 4481 5162 5353 826
Size: 219 1190 1111 1135 681 191 1471
Fragments arranged by size:
1471 1190 1135 1111 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3053 3147 3325 1872
Size: 1181 94 178 4545
Fragments arranged by size:
4545 1181 178 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 4102 5003 5265 820
Size: 197 926 78 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 2335 152
Size: 2183 3815
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2343 2474 2697 3168 3437
Size: 552 939 177 162 131 223 471 269
Cuts at: 3437 513
Size: 3074
Fragments arranged by size:
3074 939 552 471 269 223 177 162
131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2269 2333 3143 4025 4042 4195 4380
Size: 1255 689 64 810 882 17 153 185
Cuts at: 4380 4825 325
Size: 445 1498
Fragments arranged by size:
1498 1255 882 810 689 445 185 153
64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4244 5956 96
Size: 274 80 33 51 1443 2267 1712 138
Fragments arranged by size:
2267 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 2455 2487 5958 5990 2027
Size: 32 396 32 3471 32 2035
Fragments arranged by size:
3471 2035 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2300 2300
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3051 3145 3323 1870
Size: 1181 94 178 4545
Fragments arranged by size:
4545 1181 178 94
XmnI GAAnn'nnTTC
Cuts at: 3062 4127 3062
Size: 1065 4933
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI