Restriction map for
pFN21AB0962
(Circular) (Six-base) MAPSORT of: pFN21AB0962.seq Check: 6074 from: 1 to: 5980
pFN21AB0962 5980 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2729 3051 4043 5427 5695 1632
Size: 1097 322 992 1384 268 1917
Fragments arranged by size:
1917 1384 1097 992 322 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2743 3723 5295 2743
Size: 980 1572 3428
Fragments arranged by size:
3428 1572 980
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2555 2593 2879 3289 3515 3667 5776 1493
Size: 1062 38 286 410 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 410 286 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 3011 3305 1870
Size: 73 347 721 294 4545
Fragments arranged by size:
4545 721 347 294 73
BamHI G'GATC_C
Cuts at: 2544 3310 3677 2544
Size: 766 367 4847
Fragments arranged by size:
4847 766 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 3301 4756 618
Size: 325 217 627 508 1006 1455 1842
Fragments arranged by size:
1842 1455 1006 627 508 325 217
BanII G_rGCy'C
Cuts at: 1391 3012 3173 3216 3299 1391
Size: 1621 161 43 83 4072
Fragments arranged by size:
4072 1621 161 83 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2503 2717 3191 928
Size: 971 604 214 474 3717
Fragments arranged by size:
3717 971 604 474 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2387 2421 4171 4205 1903
Size: 34 450 34 1750 34 3678
Fragments arranged by size:
3678 1750 450 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3172 3872 5082 3172
Size: 700 1210 4070
Fragments arranged by size:
4070 1210 700
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BfrBI ATG'CAT
Cuts at: 2121 2121
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2734 4591 136
Size: 107 122 71 631 605 1062 1857 1525
Fragments arranged by size:
1857 1525 1062 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2103 4041 5197 1790
Size: 313 1938 1156 2573
Fragments arranged by size:
2573 1938 1156 313
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 2840 2879 4640 1239
Size: 1526 75 39 1761 2579
Fragments arranged by size:
2579 1761 1526 75 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 4105 4970 5268 2672
Size: 1433 865 298 3384
Fragments arranged by size:
3384 1433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2597 2600 3224 1186
Size: 1411 3 624 3942
Fragments arranged by size:
3942 1411 624 3
BseYI C'CCAG_C
Cuts at: 2620 2961 3104 5183 5679 2620
Size: 341 143 2079 496 2921
Fragments arranged by size:
2921 2079 496 341 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2788 1163
Size: 573 546 506 4355
Fragments arranged by size:
4355 573 546 506
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3173 3216 4041 4126 5197 3173
Size: 43 825 85 1071 3956
Fragments arranged by size:
3956 1071 825 85 43
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 3012 3173 3216 3299 4041 4126
Size: 399 313 909 161 43 83 742 85
Cuts at: 4126 5197 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 909 742 399 313 161 85
83 43
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 2626 3869 5599 2626
Size: 1243 1730 3007
Fragments arranged by size:
3007 1730 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 2494 96
Size: 2398 3582
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2725 794
Size: 1931 4049
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2439 2544 3310 3677 4062 4079 5531 5617
Size: 467 105 766 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 766 467 385 367 346 105
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 4290 4310 3496
Size: 794 20 5166
Fragments arranged by size:
5166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 2492 3230 2492
Size: 738 5242
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3427 4316
Size: 54 1425 50 275 18 177 1420 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1420 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2772 2951 3070 3910 1353
Size: 1419 179 119 840 3423
Fragments arranged by size:
3423 1419 840 179 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3171 3214 3171
Size: 43 5937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2729 2765 2840 2879 3051 4043 4640
Size: 393 1097 36 75 39 172 992 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1097 992 787 597 393 268 172
75 39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 5980
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
EcoRV GAT'ATC
Cuts at: 2752 2752
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2360 2392 2999
Size: 32 135 32 677 32 79 32 607
Cuts at: 2999 3031 3142 3174 4621 4653 4695 4727 1373
Size: 32 111 32 1447 32 42 32 2626
Fragments arranged by size:
2626 1447 677 607 135 111 79 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3981
Fragments arranged by size:
3981 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3938 4297 4456 518
Size: 648 2772 359 159 2042
Fragments arranged by size:
2772 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2740 3396 4073 5221 5466 1606
Size: 1134 656 677 1148 245 2120
Fragments arranged by size:
2120 1148 1134 677 656 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NsiI A_TGCA'T
Cuts at: 2123 2123
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2289 2728 3338 4883 1132
Size: 97 1060 439 610 1545 2229
Fragments arranged by size:
2229 1545 1060 610 439 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 4049 4081 5562 5594 2987
Size: 32 1030 32 1481 32 3373
Fragments arranged by size:
3373 1481 1030 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 2076 3028 3332 830
Size: 1246 952 304 3478
Fragments arranged by size:
3478 1246 952 304
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2740 2740
Size: 5980
SacI G_AGCT'C
Cuts at: 3173 3216 3173
Size: 43 5937
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SapI GCTCTTCn'nnn_
Cuts at: 2951 2951
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SexAI A'CCwGG_T
Cuts at: 2520 2520
Size: 5980
SfcI C'TryA_G
Cuts at: 826 1045 2072 3024 3328 4463 5144 5335 826
Size: 219 1027 952 304 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 952 681 304 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3013 3307 1872
Size: 1141 294 4545
Fragments arranged by size:
4545 1141 294
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 4084 4985 5247 820
Size: 197 926 744 1397 901 262 1553
Fragments arranged by size:
1553 1397 926 901 744 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 3419 513
Size: 552 939 360 1055 3074
Fragments arranged by size:
3074 1055 939 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2746 4007 4024 4177 4362 4807 325
Size: 1255 1166 1261 17 153 185 445 1498
Fragments arranged by size:
1498 1261 1255 1166 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2473 2494 4226
Size: 274 80 33 51 1443 496 21 1732
Cuts at: 4226 5938 96
Size: 1712 138
Fragments arranged by size:
1732 1712 1443 496 274 138 80 51
33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2740 2740
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3011 3305 1870
Size: 1141 294 4545
Fragments arranged by size:
4545 1141 294
XmnI GAAnn'nnTTC
Cuts at: 2594 2781 2879 4109 2594
Size: 187 98 1230 4465
Fragments arranged by size:
4465 1230 187 98
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI