Restriction map for
pFN21AB0958
(Circular) (Six-base) MAPSORT of: pFN21AB0958.seq Check: 2688 from: 1 to: 5965
pFN21AB0958 5965 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 2975 4028 5412 5680 1632
Size: 1154 189 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1154 1053 268 189
AflII C'TTAA_G
Cuts at: 820 1017 3236 820
Size: 197 2219 3549
Fragments arranged by size:
3549 2219 197
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 2616 1166
Size: 1450 4515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3014 1184
Size: 1830 4135
AlwNI CAG_nnn'CTG
Cuts at: 3708 5280 3708
Size: 1572 4393
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 2135 2625 3274 3500 3652 5761 1493
Size: 642 490 649 226 152 2109 1697
Fragments arranged by size:
2109 1697 649 642 490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 3290 1870
Size: 73 90 1257 4545
Fragments arranged by size:
4545 1257 90 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3286 4741 618
Size: 325 217 627 1499 1455 1842
Fragments arranged by size:
1842 1499 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2963 3284 1391
Size: 1572 321 4072
Fragments arranged by size:
4072 1572 321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2529 3164 928
Size: 971 630 635 3729
Fragments arranged by size:
3729 971 635 630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 4026 5182 1790
Size: 2236 1156 2573
Fragments arranged by size:
2573 2236 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3696 4654 442
Size: 416 53 83 399 24 2279 958 1753
Fragments arranged by size:
2279 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2882 2900 4625 1239
Size: 1643 18 1725 2579
Fragments arranged by size:
2579 1725 1643 18
Bpu10I CC'TnA_GC
Cuts at: 2307 2307
Size: 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4090 4955 5253 4090
Size: 865 298 4802
Fragments arranged by size:
4802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3772 4154 4789 275
Size: 53 83 186 936 2239 382 635 1451
Fragments arranged by size:
2239 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 4398 4796 5070 5217 5628 2055
Size: 2343 398 274 147 411 2392
Fragments arranged by size:
2392 2343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2353 2383 1178
Size: 30 165 30 950 30 4760
Fragments arranged by size:
4760 950 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2374 3122 1186
Size: 1188 748 4029
Fragments arranged by size:
4029 1188 748
BseYI C'CCAG_C
Cuts at: 2290 2721 5168 5664 2290
Size: 431 2447 496 2591
Fragments arranged by size:
2591 2447 496 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4176 5204 1540
Size: 275 186 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 4026 4111 5182 2817
Size: 1209 85 1071 3600
Fragments arranged by size:
3600 1209 1071 85
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2474 2003
Size: 471 5494
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2817 2963 3284 4026 4111 5182 1391
Size: 399 1027 146 321 742 85 1071 2174
Fragments arranged by size:
2174 1071 1027 742 399 321 146 85
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 2406 2758 3854 5584 2406
Size: 352 1096 1730 2787
Fragments arranged by size:
2787 1730 1096 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5965
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5965
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 3295 3662 4047 4064 5516 5602 5614 5960
Size: 1323 367 385 17 1452 86 12 346
Cuts at: 5960 1972
Size: 1977
Fragments arranged by size:
1977 1452 1323 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2912 513
Size: 552 939 908 3566
Fragments arranged by size:
3566 939 908 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 3481 4275 4295 3481
Size: 794 20 5151
Fragments arranged by size:
5151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2083 2118 529
Size: 35 1519 35 4376
Fragments arranged by size:
4376 1519 35 35
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2415 3412 4301
Size: 54 1425 50 275 18 585 997 889
Cuts at: 4301 5930 8
Size: 1629 43
Fragments arranged by size:
1629 1425 997 889 585 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2951 3090 3236 3895 1353
Size: 1598 139 146 659 3423
Fragments arranged by size:
3423 1598 659 146 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4552 4926 5072 387
Size: 1064 138 2963 374 146 1280
Fragments arranged by size:
2963 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2786 2882 2900 2975 4028 4625 5412
Size: 393 1154 96 18 75 1053 597 787
Cuts at: 5412 5680 1239
Size: 268 1524
Fragments arranged by size:
1524 1154 1053 787 597 393 268 96
75 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2579 2982 2579
Size: 403 5562
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
EcoRV GAT'ATC
Cuts at: 2840 2923 2840
Size: 83 5882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2467 3248 2137
Size: 330 781 4854
Fragments arranged by size:
4854 781 330
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 5112 1678
Size: 3434 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4606 4638 4680 4712 1373
Size: 32 135 32 3034 32 42 32 2626
Fragments arranged by size:
3034 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 3555 669
Size: 988 281 1371 246 3079
Fragments arranged by size:
3079 1371 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2776 3055 748
Size: 2028 279 3658
Fragments arranged by size:
3658 2028 279
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3071 5079 5263 1305
Size: 1766 2008 184 2007
Fragments arranged by size:
2008 2007 1766 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2417 10
Size: 54 1425 343 585 3558
Fragments arranged by size:
3558 1425 585 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2411 2616 2996 3923 4282 4441
Size: 648 1170 75 205 380 927 359 159
Cuts at: 4441 518
Size: 2042
Fragments arranged by size:
2042 1170 927 648 380 359 205 159
75
MspA1I CmG'CkG
Cuts at: 1606 3381 4058 5206 5451 1606
Size: 1775 677 1148 245 2120
Fragments arranged by size:
2120 1775 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4474
Fragments arranged by size:
4474 939 552
NdeI CA'TA_TG
Cuts at: 387 2384 387
Size: 1997 3968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5965
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2385 1613
Size: 772 5193
PsiI TTA'TAA
Cuts at: 3153 3535 3153
Size: 382 5583
PstI C_TGCA'G
Cuts at: 830 2380 2545 3317 830
Size: 1550 165 772 3478
Fragments arranged by size:
3478 1550 772 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SapI GCTCTTCn'nnn_
Cuts at: 2951 2951
Size: 5965
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 2081 2903 4213 5925 2081
Size: 822 1310 1712 2121
Fragments arranged by size:
2121 1712 1310 822
SfcI C'TryA_G
Cuts at: 826 1045 2376 2541 3313 4448 5129 5320 826
Size: 219 1331 165 772 1135 681 191 1471
Fragments arranged by size:
1471 1331 1135 772 681 219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3236 4069 4970 5232 820
Size: 197 926 1293 833 901 262 1553
Fragments arranged by size:
1553 1293 926 901 833 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2004 2685 3404 513
Size: 552 939 681 719 3074
Fragments arranged by size:
3074 939 719 681 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3992 4009 4162 4347 4792 325
Size: 1255 2412 17 153 185 445 1498
Fragments arranged by size:
2412 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 2901 4211
Size: 274 80 33 51 1443 102 822 1310
Cuts at: 4211 5923 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1310 822 274 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 2625 2754 3195 4094 2625
Size: 129 441 899 4496
Fragments arranged by size:
4496 899 441 129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI