Restriction map for
pFN21AB0949
(Circular) (Six-base) MAPSORT of: pFN21AB0949.seq Check: 6968 from: 1 to: 5245
pFN21AB0949 5245 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 278
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2588 1937
Size: 651 4594
Acc65I G'GTAC_C
Cuts at: 2566 2566
Size: 5245
AclI AA'CG_TT
Cuts at: 1563 2544 3372 3745 1563
Size: 981 828 373 3063
Fragments arranged by size:
3063 981 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 3308 4692 4960 1632
Size: 548 1128 1384 268 1917
Fragments arranged by size:
1917 1384 1128 548 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5048
AflIII A'CryG_T
Cuts at: 1203 4144 5121 1203
Size: 2941 977 1327
Fragments arranged by size:
2941 1327 977
AgeI A'CCGG_T
Cuts at: 4076 4076
Size: 5245
AhdI GACnn_n'nnGTC
Cuts at: 3974 3974
Size: 5245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5245
AlwNI CAG_nnn'CTG
Cuts at: 2988 4560 2988
Size: 1572 3673
ApaLI G'TGCA_C
Cuts at: 3302 4458 3302
Size: 1156 4089
ApoI r'AATT_y
Cuts at: 1493 2554 2780 2932 5041 1493
Size: 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1061 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3799 160
Size: 872 2767 1606
Fragments arranged by size:
2767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
AvaI C'yCGr_G
Cuts at: 1870 1943 2570 1870
Size: 73 627 4545
Fragments arranged by size:
4545 627 73
BamHI G'GATC_C
Cuts at: 2575 2942 2575
Size: 367 4878
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2060 2566 4021 618
Size: 325 217 627 221 52 506 1455 1842
Fragments arranged by size:
1842 1455 627 506 325 221 217 52
BanII G_rGCy'C
Cuts at: 1391 2258 2564 1391
Size: 867 306 4072
Fragments arranged by size:
4072 867 306
BbeI G_GCGC'C
Cuts at: 2012 2064 2012
Size: 52 5193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2474 928
Size: 971 575 3699
Fragments arranged by size:
3699 971 575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3436 3470 1903
Size: 34 1499 34 3678
Fragments arranged by size:
3678 1499 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2430 3137 4347 2430
Size: 707 1210 3328
Fragments arranged by size:
3328 1210 707
BclI T'GATC_A
Cuts at: 1548 2298 1548
Size: 750 4495
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 3856 136
Size: 107 122 71 631 605 386 1798 1525
Fragments arranged by size:
1798 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 5240 5240
Size: 5245
BlpI GC'TnA_GC
Cuts at: 2662 2662
Size: 5245
Bme1580I G_kGCm'C
Cuts at: 1790 3306 4462 1790
Size: 1516 1156 2573
Fragments arranged by size:
2573 1516 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2207 2976 3934
Size: 416 53 83 399 24 790 769 958
Cuts at: 3934 442
Size: 1753
Fragments arranged by size:
1753 958 790 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 3905 1239
Size: 823 1843 2579
Fragments arranged by size:
2579 1843 823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4235 4533 3370
Size: 865 298 4082
Fragments arranged by size:
4082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3908 882
Size: 617 2409 2219
Fragments arranged by size:
2409 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3036 493
Size: 711 1832 2702
Fragments arranged by size:
2702 1832 711
BsaBI GATnn'nnATC
Cuts at: 5239 5239
Size: 5245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2033 2061 3052
Size: 53 83 186 936 476 24 28 991
Cuts at: 3052 3434 4069 275
Size: 382 635 1451
Fragments arranged by size:
1451 991 936 635 476 382 186 83
53 28 24
BsaWI w'CCGG_w
Cuts at: 3678 4076 4350 4497 4908 3678
Size: 398 274 147 411 4015
Fragments arranged by size:
4015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5020
Fragments arranged by size:
5020 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2392 2417 2441 1186
Size: 1135 71 25 24 3990
Fragments arranged by size:
3990 1135 71 25 24
BseYI C'CCAG_C
Cuts at: 2265 4448 4944 2265
Size: 2183 496 2566
Fragments arranged by size:
2566 2183 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2244 1163
Size: 573 508 4164
Fragments arranged by size:
4164 573 508
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 3456 4484 1540
Size: 275 186 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 3306 3391 4462 2258
Size: 1048 85 1071 3041
Fragments arranged by size:
3041 1071 1048 85
BsmI GAATG_Cn'
Cuts at: 2756 2849 2756
Size: 93 5152
BsmBI CGTCTCn'nnnn_
Cuts at: 2173 2173
Size: 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2564 3306 3391 4462 1391
Size: 399 468 306 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 306 85
BspEI T'CCGG_A
Cuts at: 4908 4908
Size: 5245
BspHI T'CATG_A
Cuts at: 3134 4864 3134
Size: 1730 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2600 844
Size: 1756 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2422 3132 1394
Size: 429 599 710 3507
Fragments arranged by size:
3507 710 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2340 3740 3914 65
Size: 1183 1092 1400 174 1396
Fragments arranged by size:
1400 1396 1183 1092 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2233 3889 4076 1700
Size: 110 423 1656 187 2869
Fragments arranged by size:
2869 1656 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5245
BssSI C'ACGA_G
Cuts at: 3305 4317 3305
Size: 1012 4233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5245
BstBI TT'CG_AA
Cuts at: 4049 4049
Size: 5245
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5245
BstYI r'GATC_y
Cuts at: 1972 2575 2942 3327 3344 4796 4882 4894 5240
Size: 603 367 385 17 1452 86 12 346
Cuts at: 5240 1972
Size: 1977
Fragments arranged by size:
1977 1452 603 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2023 513
Size: 552 939 19 3735
Fragments arranged by size:
3735 939 552 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4506
Fragments arranged by size:
4506 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3555 3575 2761
Size: 794 20 4431
Fragments arranged by size:
4431 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5210
DraI TTT'AAA
Cuts at: 2509 2549 2509
Size: 40 5205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 809
Size: 507 2936 1802
Fragments arranged by size:
2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2692 3581
Size: 54 1425 50 275 18 261 601 889
Cuts at: 3581 5210 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 601 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2692 1537
Size: 275 880 4090
Fragments arranged by size:
4090 880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2369 3175 1353
Size: 792 224 806 3423
Fragments arranged by size:
3423 806 792 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2449 3832 4206 4352 387
Size: 1064 138 860 1383 374 146 1280
Fragments arranged by size:
1383 1280 1064 860 374 146 138
EcoICRI GAG'CTC
Cuts at: 2256 2256
Size: 5245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2180 3308 3905 4692 4960 1239
Size: 393 430 118 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 787 597 430 393 268 118
EcoRI G'AATT_C
Cuts at: 2554 2932 2554
Size: 378 4867
FspI TGC'GCA
Cuts at: 3751 3751
Size: 5245
HaeII r_GCGC'y
Cuts at: 1678 2012 2064 2297 4392 1678
Size: 334 52 233 2095 2531
Fragments arranged by size:
2531 2095 334 233 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3886 3918 3960 3992 1373
Size: 32 135 32 2314 32 42 32 2626
Fragments arranged by size:
2626 2314 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2589 2835 669
Size: 988 281 651 246 3079
Fragments arranged by size:
3079 988 651 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5245
HpaI GTT'AAC
Cuts at: 2835 2835
Size: 5245
KasI G'GCGC_C
Cuts at: 2008 2060 2008
Size: 52 5193
KpnI G_GTAC'C
Cuts at: 2570 2570
Size: 5245
MfeI C'AATT_G
Cuts at: 2844 2844
Size: 5245
MluI A'CGCG_T
Cuts at: 5121 5121
Size: 5245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4359 4543 1305
Size: 3054 184 2007
Fragments arranged by size:
3054 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3423
Fragments arranged by size:
3423 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2306 3203 3562 3721 518
Size: 648 921 219 897 359 159 2042
Fragments arranged by size:
2042 921 897 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2661 3338 4486 4731 1606
Size: 419 636 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 419 245
NarI GG'CG_CC
Cuts at: 2009 2061 2009
Size: 52 5193
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3754
Fragments arranged by size:
3754 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5245
NotI GC'GGCC_GC
Cuts at: 2692 2692
Size: 5245
NspI r_CATG'y
Cuts at: 1132 1229 2603 4148 1132
Size: 97 1374 1545 2229
Fragments arranged by size:
2229 1545 1374 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5245
PciI A'CATG_T
Cuts at: 4144 4144
Size: 5245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5245
PfoI T'CCnGG_A
Cuts at: 2110 2110
Size: 5245
PmeI GTTT'AAAC
Cuts at: 2549 2549
Size: 5245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 4827 4859 3314
Size: 32 1481 32 3700
Fragments arranged by size:
3700 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5245
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5245
PstI C_TGCA'G
Cuts at: 830 2369 2597 830
Size: 1539 228 3478
Fragments arranged by size:
3478 1539 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5245
SacI G_AGCT'C
Cuts at: 2258 2258
Size: 5245
SacII CC_GC'GG
Cuts at: 2026 2026
Size: 5245
SalI G'TCGA_C
Cuts at: 1936 2587 1936
Size: 651 4594
SbfI CC_TGCA'GG
Cuts at: 2597 2597
Size: 5245
ScaI AGT'ACT
Cuts at: 3493 5205 3493
Size: 1712 3533
SfcI C'TryA_G
Cuts at: 826 1045 2365 2593 3728 4409 4600 826
Size: 219 1320 228 1135 681 191 1471
Fragments arranged by size:
1471 1320 1135 681 228 219 191
SfoI GGC'GCC
Cuts at: 2010 2062 2010
Size: 52 5193
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5245
SmaI CCC'GGG
Cuts at: 1872 2572 1872
Size: 700 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3349 4250 4512 820
Size: 197 926 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3036 493
Size: 2543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5245
SphI G_CATG'C
Cuts at: 1132 2603 1132
Size: 1471 3774
SspI AAT'ATT
Cuts at: 5 52 3169 5
Size: 47 3117 2081
Fragments arranged by size:
3117 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5245
StyI C'CwwG_G
Cuts at: 513 1065 2004 2684 513
Size: 552 939 680 3074
Fragments arranged by size:
3074 939 680 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2269 3272 3289 3442 3627 4072 325
Size: 1255 689 1003 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1003 689 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3491 5203 96
Size: 274 80 33 51 1443 1514 1712 138
Fragments arranged by size:
1712 1514 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5205 5237 5205
Size: 32 5213
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5245
XbaI T'CTAG_A
Cuts at: 2581 2581
Size: 5245
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5245
XmaI C'CCGG_G
Cuts at: 1870 2570 1870
Size: 700 4545
XmnI GAAnn'nnTTC
Cuts at: 3374 3374
Size: 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 276
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI
XcmI