Restriction map for
pFN21AB0946
(Circular) (Six-base) MAPSORT of: pFN21AB0946.seq Check: 6624 from: 1 to: 5902
pFN21AB0946 5902 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2399 3245 1937
Size: 462 846 4594
Fragments arranged by size:
4594 846 462
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 3201 3965 5349 5617 1632
Size: 819 750 764 1384 268 1917
Fragments arranged by size:
1917 1384 819 764 750 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3645 5217 3645
Size: 1572 4330
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2327 2335 2347 2437 2467 2527 2667 2919
Size: 834 8 12 90 30 60 140 252
Cuts at: 2919 3087 3211 3437 3589 5698 1493
Size: 168 124 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 252 226 168 152 140
124 90 60 30 12 8
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 3227 1870
Size: 73 1284 4545
Fragments arranged by size:
4545 1284 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 3221 1391
Size: 799 1031 4072
Fragments arranged by size:
4072 1031 799
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2717 928
Size: 971 818 4113
Fragments arranged by size:
4113 971 818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 5290
BfrBI ATG'CAT
Cuts at: 2781 2781
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2583 2583
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2683 3033 4562 1239
Size: 1444 350 1529 2579
Fragments arranged by size:
2579 1529 1444 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3113 4027 4892 5190 3113
Size: 914 865 298 3825
Fragments arranged by size:
3825 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2131 4565 882
Size: 617 632 2434 2219
Fragments arranged by size:
2434 2219 632 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5902
BseYI C'CCAG_C
Cuts at: 5105 5601 5105
Size: 496 5406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2837 3963 4048 5119 2837
Size: 1126 85 1071 3620
Fragments arranged by size:
3620 1126 1071 85
BsmI GAATG_Cn'
Cuts at: 2369 3119 3413 3506 2369
Size: 750 294 93 4765
Fragments arranged by size:
4765 750 294 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2988 2988
Size: 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2837 3221 3963 4048 5119 1391
Size: 399 400 647 384 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 647 400 399 384 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 2047 3791 5521 2047
Size: 1744 1730 2428
Fragments arranged by size:
2428 1744 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897
Size: 1260 367 385 17 1452 86 12 346
Cuts at: 5897 1972
Size: 1977
Fragments arranged by size:
1977 1452 1260 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2722 2806 513
Size: 552 1657 84 3609
Fragments arranged by size:
3609 1657 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2620 3418 4212 4232 2620
Size: 798 794 20 4290
Fragments arranged by size:
4290 798 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 2526 3206 2526
Size: 680 5222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867
Size: 54 1425 50 275 18 1519 889 1629
Cuts at: 5867 8
Size: 43
Fragments arranged by size:
1629 1519 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 3832 1353
Size: 693 1786 3423
Fragments arranged by size:
3423 1786 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2683 3033 3201 3965 4562 5349
Size: 393 819 232 350 168 764 597 787
Cuts at: 5349 5617 1239
Size: 268 1524
Fragments arranged by size:
1524 819 787 764 597 393 350 268
232 168
EcoRI G'AATT_C
Cuts at: 2437 2667 2919 3087 3211 3589 2437
Size: 230 252 168 124 378 4750
Fragments arranged by size:
4750 378 252 230 168 124
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2586 2618 4543
Size: 32 135 32 638 32 344 32 1925
Cuts at: 4543 4575 4617 4649 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1925 638 344 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3048 748
Size: 2300 3602
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2448 3860 4219 4378 518
Size: 648 1282 1412 359 159 2042
Fragments arranged by size:
2042 1412 1282 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3318 3995 5143 5388 1606
Size: 1712 677 1148 245 2120
Fragments arranged by size:
2120 1712 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2722 2806 513
Size: 552 1657 84 3609
Fragments arranged by size:
3609 1657 552 84
NdeI CA'TA_TG
Cuts at: 387 2155 387
Size: 1768 4134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NsiI A_TGCA'T
Cuts at: 2783 2783
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2064 2613 3260 4805 1132
Size: 97 835 549 647 1545 2229
Fragments arranged by size:
2229 1545 835 647 549 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 3971 4003 5484 5516 2111
Size: 32 1828 32 1481 32 2497
Fragments arranged by size:
2497 1828 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 2489 3472 2489
Size: 983 4919
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 5902
SfcI C'TryA_G
Cuts at: 826 1045 2034 2130 3058 3250 4385 5066 5257
Size: 219 989 96 928 192 1135 681 191
Cuts at: 5257 826
Size: 1471
Fragments arranged by size:
1471 1135 989 928 681 219 192 191
96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3128 4006 4907 5169 820
Size: 197 926 1185 878 901 262 1553
Fragments arranged by size:
1553 1185 926 901 878 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 2064 3260 1132
Size: 932 1196 3774
Fragments arranged by size:
3774 1196 932
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 2966 1517
Size: 1281 168 4453
Fragments arranged by size:
4453 1281 168
StyI C'CwwG_G
Cuts at: 513 1065 2722 2806 3341 513
Size: 552 1657 84 535 3074
Fragments arranged by size:
3074 1657 552 535 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 3946 4099 4284 4729 325
Size: 1255 2349 17 153 185 445 1498
Fragments arranged by size:
2349 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 3050 4031 3050
Size: 981 4921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI