Restriction map for
pFN21AB0943
(Circular) (Six-base) MAPSORT of: pFN21AB0943.seq Check: 5843 from: 1 to: 5215
pFN21AB0943 5215 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3025 278
Size: 53 83 186 936 1489 2468
Fragments arranged by size:
2468 1489 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2558 1937
Size: 621 4594
Acc65I G'GTAC_C
Cuts at: 2536 2536
Size: 5215
AclI AA'CG_TT
Cuts at: 1563 3342 3715 1563
Size: 1779 373 3063
Fragments arranged by size:
3063 1779 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 3278 4662 4930 1632
Size: 576 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1070 576 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5018
AflIII A'CryG_T
Cuts at: 1203 4114 5091 1203
Size: 2911 977 1327
Fragments arranged by size:
2911 1327 977
AgeI A'CCGG_T
Cuts at: 4046 4046
Size: 5215
AhdI GACnn_n'nnGTC
Cuts at: 3944 3944
Size: 5215
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5215
AlwNI CAG_nnn'CTG
Cuts at: 2018 2210 2958 4530 2018
Size: 192 748 1572 2703
Fragments arranged by size:
2703 1572 748 192
ApaI G_GGCC'C
Cuts at: 2424 2424
Size: 5215
ApaLI G'TGCA_C
Cuts at: 2138 3272 4428 2138
Size: 1134 1156 2925
Fragments arranged by size:
2925 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2524 2750 2902 5011 1493
Size: 1031 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3769 160
Size: 872 2737 1606
Fragments arranged by size:
2737 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
AvaI C'yCGr_G
Cuts at: 1870 1943 2540 1870
Size: 73 597 4545
Fragments arranged by size:
4545 597 73
AvrII C'CTAG_G
Cuts at: 2056 2056
Size: 5215
BamHI G'GATC_C
Cuts at: 2545 2912 2545
Size: 367 4848
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2536 3991 618
Size: 325 217 627 749 1455 1842
Fragments arranged by size:
1842 1455 749 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2273 2424 2534 1391
Size: 882 151 110 4072
Fragments arranged by size:
4072 882 151 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 928
Size: 971 297 3947
Fragments arranged by size:
3947 971 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3406 3440 1903
Size: 34 1469 34 3678
Fragments arranged by size:
3678 1469 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3107 4317 3107
Size: 1210 4005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2259 2268 3826
Size: 107 122 71 631 605 587 9 1558
Cuts at: 3826 136
Size: 1525
Fragments arranged by size:
1558 1525 631 605 587 122 107 71
9
BglII A'GATC_T
Cuts at: 5210 5210
Size: 5215
BlpI GC'TnA_GC
Cuts at: 2632 2632
Size: 5215
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2424 3276 4432 1790
Size: 352 282 852 1156 2573
Fragments arranged by size:
2573 1156 852 352 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2946 3904 442
Size: 416 53 83 399 24 1529 958 1753
Fragments arranged by size:
1753 1529 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 3875 1239
Size: 1115 1521 2579
Fragments arranged by size:
2579 1521 1115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3340 4205 4503 3340
Size: 865 298 4052
Fragments arranged by size:
4052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3878 882
Size: 617 2379 2219
Fragments arranged by size:
2379 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3006 493
Size: 711 1802 2702
Fragments arranged by size:
2702 1802 711
BsaBI GATnn'nnATC
Cuts at: 5209 5209
Size: 5215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3022 3404 4039 275
Size: 53 83 186 936 1489 382 635 1451
Fragments arranged by size:
1489 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3648 4046 4320 4467 4878 3648
Size: 398 274 147 411 3985
Fragments arranged by size:
3985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4990
Fragments arranged by size:
4990 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5215
BseYI C'CCAG_C
Cuts at: 2276 2425 4418 4914 2276
Size: 149 1993 496 2577
Fragments arranged by size:
2577 1993 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 1163
Size: 573 297 4345
Fragments arranged by size:
4345 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 3426 4454 1540
Size: 275 186 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2218 2302 2382 3276 3361 4432 2142
Size: 76 84 80 894 85 1071 2925
Fragments arranged by size:
2925 1071 894 85 84 80 76
BsmI GAATG_Cn'
Cuts at: 2726 2819 2726
Size: 93 5122
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2218 2273 2302 2382 2424 2534
Size: 399 352 76 55 29 80 42 110
Cuts at: 2534 3276 3361 4432 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 110 85 80
76 55 42 29
BspEI T'CCGG_A
Cuts at: 4878 4878
Size: 5215
BspHI T'CATG_A
Cuts at: 2025 2155 3104 4834 2025
Size: 130 949 1730 2406
Fragments arranged by size:
2406 1730 949 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2570 844
Size: 1726 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3102 1394
Size: 429 1279 3507
Fragments arranged by size:
3507 1279 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3710 3884 65
Size: 1183 2462 174 1396
Fragments arranged by size:
2462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3859 4046 1700
Size: 110 2049 187 2869
Fragments arranged by size:
2869 2049 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5215
BssSI C'ACGA_G
Cuts at: 2304 3275 4287 2304
Size: 971 1012 3232
Fragments arranged by size:
3232 1012 971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5215
BstBI TT'CG_AA
Cuts at: 2109 4019 2109
Size: 1910 3305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5215
BstYI r'GATC_y
Cuts at: 1972 2500 2545 2912 3297 3314 4766 4852 4864
Size: 528 45 367 385 17 1452 86 12
Cuts at: 4864 5210 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 528 385 367 346 86 45
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 3631
Fragments arranged by size:
3631 1032 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4476
Fragments arranged by size:
4476 683 56
BtsI GCAGTG_nn'
Cuts at: 2505 2731 3525 3545 2505
Size: 226 794 20 4175
Fragments arranged by size:
4175 794 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5180
DraI TTT'AAA
Cuts at: 2519 2519
Size: 5215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4222 809
Size: 507 2906 1802
Fragments arranged by size:
2906 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2222 2260
Size: 54 1425 50 275 18 301 91 38
Cuts at: 2260 2662 3551 5180 8
Size: 402 889 1629 43
Fragments arranged by size:
1629 1425 889 402 301 275 91 54
50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2662 1537
Size: 275 850 4090
Fragments arranged by size:
4090 850 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3145 1353
Size: 737 1055 3423
Fragments arranged by size:
3423 1055 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3802 4176 4322 387
Size: 1064 138 2213 374 146 1280
Fragments arranged by size:
2213 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2354 3278 3875 4662 4930 1239
Size: 393 576 146 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 576 393 268 146
EcoNI CCTnn'n_nnAGG
Cuts at: 2392 2392
Size: 5215
EcoO109I rG'GnC_Cy
Cuts at: 2169 2421 2169
Size: 252 4963
EcoRI G'AATT_C
Cuts at: 2524 2902 2524
Size: 378 4837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2250 2250
Size: 5215
FspI TGC'GCA
Cuts at: 3721 3721
Size: 5215
HaeII r_GCGC'y
Cuts at: 1678 4362 1678
Size: 2684 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3856 3888 3930 3962 1373
Size: 32 135 32 2284 32 42 32 2626
Fragments arranged by size:
2626 2284 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2559 2805 669
Size: 988 281 621 246 3079
Fragments arranged by size:
3079 988 621 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5215
HpaI GTT'AAC
Cuts at: 2805 2805
Size: 5215
KpnI G_GTAC'C
Cuts at: 2540 2540
Size: 5215
MfeI C'AATT_G
Cuts at: 2814 2814
Size: 5215
MluI A'CGCG_T
Cuts at: 5091 5091
Size: 5215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4329 4513 1305
Size: 3024 184 2007
Fragments arranged by size:
3024 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2224 2262 10
Size: 54 1425 343 392 38 2963
Fragments arranged by size:
2963 1425 392 343 54 38
MslI CAynn'nnrTG
Cuts at: 518 1166 2133 3173 3532 3691 518
Size: 648 967 1040 359 159 2042
Fragments arranged by size:
2042 1040 967 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2631 3308 4456 4701 1606
Size: 1025 677 1148 245 2120
Fragments arranged by size:
2120 1148 1025 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 3631
Fragments arranged by size:
3631 1032 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5215
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5215
NotI GC'GGCC_GC
Cuts at: 2662 2662
Size: 5215
NspI r_CATG'y
Cuts at: 1132 1229 2573 4118 1132
Size: 97 1344 1545 2229
Fragments arranged by size:
2229 1545 1344 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5215
PciI A'CATG_T
Cuts at: 4114 4114
Size: 5215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2237 2486 1108
Size: 1129 249 3837
Fragments arranged by size:
3837 1129 249
PmeI GTTT'AAAC
Cuts at: 2519 2519
Size: 5215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2000 2032 3284 3316 4797 4829 2000
Size: 32 1252 32 1481 32 2386
Fragments arranged by size:
2386 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2169 2169
Size: 5215
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5215
PsiI TTA'TAA
Cuts at: 2785 2785
Size: 5215
PspOMI G'GGCC_C
Cuts at: 2420 2420
Size: 5215
PstI C_TGCA'G
Cuts at: 830 2567 830
Size: 1737 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5215
SalI G'TCGA_C
Cuts at: 1936 2557 1936
Size: 621 4594
SbfI CC_TGCA'GG
Cuts at: 2567 2567
Size: 5215
ScaI AGT'ACT
Cuts at: 3463 5175 3463
Size: 1712 3503
SexAI A'CCwGG_T
Cuts at: 2209 2209
Size: 5215
SfcI C'TryA_G
Cuts at: 826 1045 2019 2563 3698 4379 4570 826
Size: 219 974 544 1135 681 191 1471
Fragments arranged by size:
1471 1135 974 681 544 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5215
SmaI CCC'GGG
Cuts at: 1872 2542 1872
Size: 670 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3319 4220 4482 820
Size: 197 926 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3006 493
Size: 2513 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5215
SphI G_CATG'C
Cuts at: 1132 2573 1132
Size: 1441 3774
SspI AAT'ATT
Cuts at: 5 52 3139 5
Size: 47 3087 2081
Fragments arranged by size:
3087 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5215
StyI C'CwwG_G
Cuts at: 513 1065 2056 2097 2225 2393 2654 513
Size: 552 991 41 128 168 261 3074
Fragments arranged by size:
3074 991 552 261 168 128 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 3242 3259 3412 3597 4042 325
Size: 1255 599 1063 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1063 599 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3461 5173 96
Size: 274 80 33 51 1443 1484 1712 138
Fragments arranged by size:
1712 1484 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5175 5207 5175
Size: 32 5183
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5215
XbaI T'CTAG_A
Cuts at: 2551 2551
Size: 5215
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5215
XmaI C'CCGG_G
Cuts at: 1870 2540 1870
Size: 670 4545
XmnI GAAnn'nnTTC
Cuts at: 3344 3344
Size: 5215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3023 276
Size: 53 83 186 936 1489 2468
Fragments arranged by size:
2468 1489 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI