Restriction map for
pFN21AB0942
(Circular) (Six-base) MAPSORT of: pFN21AB0942.seq Check: 269 from: 1 to: 5881
pFN21AB0942 5881 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 3944 5328 5596 1632
Size: 740 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 740 268
AflII C'TTAA_G
Cuts at: 820 1017 2831 820
Size: 197 1814 3870
Fragments arranged by size:
3870 1814 197
AflIII A'CryG_T
Cuts at: 1203 2754 2894 4780 5757 1203
Size: 1551 140 1886 977 1327
Fragments arranged by size:
1886 1551 1327 977 140
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 2710 1166
Size: 1544 4337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2150 2651 2666 3624 5196 2150
Size: 501 15 958 1572 2835
Fragments arranged by size:
2835 1572 958 501 15
ApaLI G'TGCA_C
Cuts at: 3017 3938 5094 3017
Size: 921 1156 3804
Fragments arranged by size:
3804 1156 921
ApoI r'AATT_y
Cuts at: 1493 2270 2622 2636 2723 2769 3190 3416 3568
Size: 777 352 14 87 46 421 226 152
Cuts at: 3568 5677 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 777 421 352 226 152 87
46 14
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 3206 1870
Size: 73 123 1140 4545
Fragments arranged by size:
4545 1140 123 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2600 3202 4657 618
Size: 325 217 627 813 602 1455 1842
Fragments arranged by size:
1842 1455 813 627 602 325 217
BanII G_rGCy'C
Cuts at: 1391 2067 2236 3200 1391
Size: 676 169 964 4072
Fragments arranged by size:
4072 964 676 169
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4492 136
Size: 107 122 71 631 605 2820 1525
Fragments arranged by size:
2820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 3021 3942 5098 1790
Size: 1231 921 1156 2573
Fragments arranged by size:
2573 1231 1156 921
BmgBI CAC'GTC
Cuts at: 1318 2895 1318
Size: 1577 4304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 3612 4570
Size: 416 53 83 399 24 767 1428 958
Cuts at: 4570 442
Size: 1753
Fragments arranged by size:
1753 1428 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 2931 4541 1239
Size: 1449 243 1610 2579
Fragments arranged by size:
2579 1610 1449 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 4912
Fragments arranged by size:
4912 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2544 1186
Size: 1358 4523
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3021 3942 4027 5098 3021
Size: 921 85 1071 3804
Fragments arranged by size:
3804 1071 921 85
BsmI GAATG_Cn'
Cuts at: 2351 2477 3392 3485 2351
Size: 126 915 93 4747
Fragments arranged by size:
4747 915 126 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2236 3021 3200 3942 4027 5098
Size: 399 277 169 785 179 742 85 1071
Cuts at: 5098 1391
Size: 2174
Fragments arranged by size:
2174 1071 785 742 399 277 179 169
85
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 2734 3770 5500 2734
Size: 1036 1730 3115
Fragments arranged by size:
3115 1730 1036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 3169 3236 844
Size: 1179 1146 67 3489
Fragments arranged by size:
3489 1179 1146 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2303 3768 1394
Size: 429 480 1465 3507
Fragments arranged by size:
3507 1465 480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2096 4376 4550 65
Size: 1183 848 2280 174 1396
Fragments arranged by size:
2280 1396 1183 848 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 2053 96
Size: 1957 3924
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 2084 3941 4953 2084
Size: 1857 1012 3012
Fragments arranged by size:
3012 1857 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 2380 1184
Size: 1196 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876
Size: 1239 367 385 17 1452 86 12 346
Cuts at: 5876 1972
Size: 1977
Fragments arranged by size:
1977 1452 1239 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4390
Fragments arranged by size:
4390 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 2127 2583 2667 3397 4191 4211 2127
Size: 456 84 730 794 20 3797
Fragments arranged by size:
3797 794 730 456 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 2728 3185 2728
Size: 457 5424
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846
Size: 54 1425 50 275 18 1498 889 1629
Cuts at: 5846 8
Size: 43
Fragments arranged by size:
1629 1498 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3113 3143 3811 1353
Size: 1760 30 668 3423
Fragments arranged by size:
3423 1760 668 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2688 2931 3944 4541 5328 5596
Size: 393 740 316 243 1013 597 787 268
Cuts at: 5596 1239
Size: 1524
Fragments arranged by size:
1524 1013 787 740 597 393 316 268
243
EcoO109I rG'GnC_Cy
Cuts at: 2035 2035
Size: 5881
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
EcoRV GAT'ATC
Cuts at: 3108 3108
Size: 5881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2957 2957
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 4522 4554 4596
Size: 32 135 32 1001 32 1917 32 42
Cuts at: 4596 4628 1373
Size: 32 2626
Fragments arranged by size:
2626 1917 1001 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2828 748
Size: 2080 3801
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 4995 5179 1305
Size: 1815 1875 184 2007
Fragments arranged by size:
2007 1875 1815 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2202 2247 2710 2819 3839 4198 4357
Size: 648 1036 45 463 109 1020 359 159
Cuts at: 4357 518
Size: 2042
Fragments arranged by size:
2042 1036 1020 648 463 359 159 109
45
MspA1I CmG'CkG
Cuts at: 1606 2951 3297 3974 5122 5367 1606
Size: 1345 346 677 1148 245 2120
Fragments arranged by size:
2120 1345 1148 677 346 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4390
Fragments arranged by size:
4390 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2758 3239 4784 1132
Size: 97 1529 481 1545 2229
Fragments arranged by size:
2229 1545 1529 481 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 2754 4780 2754
Size: 2026 3855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2035
Size: 5881
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 2684 3451 2684
Size: 767 5114
PstI C_TGCA'G
Cuts at: 830 2956 3233 830
Size: 2126 277 3478
Fragments arranged by size:
3478 2126 277
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2951 2951
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2952 3229 4364 5045 5236 826
Size: 219 1907 277 1135 681 191 1471
Fragments arranged by size:
1907 1471 1135 681 277 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 3985 4886 5148 820
Size: 197 926 888 1154 901 262 1553
Fragments arranged by size:
1553 1154 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 2707 152
Size: 2555 3326
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 3320 513
Size: 552 939 1316 3074
Fragments arranged by size:
3074 1316 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2415 3908 3925 4078 4263 4708 325
Size: 1255 835 1493 17 153 185 445 1498
Fragments arranged by size:
1498 1493 1255 835 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2053 4127 5839
Size: 274 80 33 51 1443 76 2074 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
2074 1712 1443 274 138 80 76 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 5841 5873 2117
Size: 32 3692 32 2125
Fragments arranged by size:
3692 2125 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 2193 2940 4010 2193
Size: 747 1070 4064
Fragments arranged by size:
4064 1070 747
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI