Restriction map for
pFN21AB0922
(Circular) (Six-base) MAPSORT of: pFN21AB0922.seq Check: 4086 from: 1 to: 5740
pFN21AB0922 5740 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 2945 3061 2945
Size: 116 5624
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3016 3803 5187 5455 1632
Size: 1384 787 1384 268 1917
Fragments arranged by size:
1917 1384 1384 787 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2552 2975 1184
Size: 1368 423 3949
Fragments arranged by size:
3949 1368 423
AlwNI CAG_nnn'CTG
Cuts at: 2557 2813 3483 5055 2557
Size: 256 670 1572 3242
Fragments arranged by size:
3242 1572 670 256
ApaI G_GGCC'C
Cuts at: 2245 2259 2907 2245
Size: 14 648 5078
Fragments arranged by size:
5078 648 14
ApaLI G'TGCA_C
Cuts at: 2150 3797 4953 2150
Size: 1647 1156 2937
Fragments arranged by size:
2937 1647 1156
ApoI r'AATT_y
Cuts at: 1493 2066 3049 3275 3427 5536 1493
Size: 573 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 573 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2161 2648 3065 1870
Size: 73 218 487 417 4545
Fragments arranged by size:
4545 487 417 218 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2368 2945 3061 4516 618
Size: 325 217 627 581 577 116 1455 1842
Fragments arranged by size:
1842 1455 627 581 577 325 217 116
BanII G_rGCy'C
Cuts at: 1391 2245 2259 2352 2907 3059 1391
Size: 854 14 93 555 152 4072
Fragments arranged by size:
4072 854 555 152 93 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2684 928
Size: 971 785 3984
Fragments arranged by size:
3984 971 785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2508 4351 136
Size: 107 122 71 631 605 836 1843 1525
Fragments arranged by size:
1843 1525 836 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 2823 3157 2823
Size: 334 5406
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2245 2259 2907 3801 4957 1790
Size: 364 91 14 648 894 1156 2573
Fragments arranged by size:
2573 1156 894 648 364 91 14
BmgBI CAC'GTC
Cuts at: 1318 2173 2443 1318
Size: 855 270 4615
Fragments arranged by size:
4615 855 270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3471 4429
Size: 416 53 83 399 24 1457 597 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1457 958 597 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2284 2579 4400 1239
Size: 1045 295 1821 2579
Fragments arranged by size:
2579 1821 1045 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 4403 882
Size: 617 954 1950 2219
Fragments arranged by size:
2219 1950 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 2597 3531 493
Size: 711 1393 934 2702
Fragments arranged by size:
2702 1393 934 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 3547 3929 4564
Size: 53 83 186 936 540 1474 382 635
Cuts at: 4564 275
Size: 1451
Fragments arranged by size:
1474 1451 936 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2782 2812 1178
Size: 30 165 30 1379 30 4106
Fragments arranged by size:
4106 1379 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3030 1186
Size: 1844 3896
BseYI C'CCAG_C
Cuts at: 2913 2997 4943 5439 2913
Size: 84 1946 496 3214
Fragments arranged by size:
3214 1946 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2300 2465 2906 1163
Size: 573 483 81 165 441 3997
Fragments arranged by size:
3997 573 483 441 165 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 3190 3951 4979 1540
Size: 275 186 14 1175 761 1028 2301
Fragments arranged by size:
2301 1175 1028 761 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 2154 3801 3886 4957 2154
Size: 1647 85 1071 2937
Fragments arranged by size:
2937 1647 1071 85
BsmI GAATG_Cn'
Cuts at: 3009 3251 3344 3009
Size: 242 93 5405
Fragments arranged by size:
5405 242 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2245 2259 2352 2907 3059 3801
Size: 399 364 91 14 93 555 152 742
Cuts at: 3801 3886 4957 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 399 364 152 93
91 85 14
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2928 3095 844
Size: 1370 714 167 3489
Fragments arranged by size:
3489 1370 714 167
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 2563 3627 1394
Size: 429 292 448 1064 3507
Fragments arranged by size:
3507 1064 448 429 292
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 5635
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 2308
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2157 2643 3070 3437 3822 3839 5291 5377
Size: 185 486 427 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 486 427 385 367 346 185
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4249
Fragments arranged by size:
4249 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 2264 2854 3256 4050 4070 2264
Size: 590 402 794 20 3934
Fragments arranged by size:
3934 794 590 402 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2247 2360
Size: 54 1425 50 275 18 182 235 113
Cuts at: 2360 2384 3187 4076 5705 8
Size: 24 803 889 1629 43
Fragments arranged by size:
1629 1425 889 803 275 235 182 113
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2012 3187 1537
Size: 275 200 1175 4090
Fragments arranged by size:
4090 1175 275 200
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 3670 1353
Size: 701 1616 3423
Fragments arranged by size:
3423 1616 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 4327 4701 4847 387
Size: 1064 138 819 1919 374 146 1280
Fragments arranged by size:
1919 1280 1064 819 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2284 2579 3016 3803 4400 5187 5455
Size: 393 652 295 437 787 597 787 268
Cuts at: 5455 1239
Size: 1524
Fragments arranged by size:
1524 787 787 652 597 437 393 295
268
EcoO109I rG'GnC_Cy
Cuts at: 2242 2256 2554 2795 2810 2903 2904 2917 2242
Size: 14 298 241 15 93 1 13 5065
Fragments arranged by size:
5065 298 241 93 15 14 13 1
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 2378 4246 2378
Size: 1868 3872
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2782 2814 4381 4413 4455
Size: 32 135 32 1210 32 1567 32 42
Cuts at: 4455 4487 1373
Size: 32 2626
Fragments arranged by size:
2626 1567 1210 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 2949 3065 2949
Size: 116 5624
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2107 2346 4854 5038 1305
Size: 802 239 2508 184 2007
Fragments arranged by size:
2508 2007 802 239 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 10
Size: 54 1425 343 417 3501
Fragments arranged by size:
3501 1425 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2487 2534 3698 4057 4216 518
Size: 648 1275 46 47 1164 359 159 2042
Fragments arranged by size:
2042 1275 1164 648 359 159 47 46
MspA1I CmG'CkG
Cuts at: 1606 2253 2304 2335 3156 3833 4981 5226 1606
Size: 647 51 31 821 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 647 245 51 31
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4249
Fragments arranged by size:
4249 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2506 2551 1348
Size: 1158 45 4537
Fragments arranged by size:
4537 1158 45
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 1108
Size: 1449 4291
PfoI T'CCnGG_A
Cuts at: 2121 2569 2121
Size: 448 5292
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2795 2917 2795
Size: 122 5618
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PspOMI G'GGCC_C
Cuts at: 2241 2255 2903 2241
Size: 14 648 5078
Fragments arranged by size:
5078 648 14
PspXI vC'TCGA_Gb
Cuts at: 2648 2648
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2334 2809 3092 830
Size: 1504 475 283 3478
Fragments arranged by size:
3478 1504 475 283
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SexAI A'CCwGG_T
Cuts at: 2853 2853
Size: 5740
SfcI C'TryA_G
Cuts at: 826 1045 2330 2805 3088 4223 4904 5095 826
Size: 219 1285 475 283 1135 681 191 1471
Fragments arranged by size:
1471 1285 1135 681 475 283 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 2163 3067 1872
Size: 291 904 4545
Fragments arranged by size:
4545 904 291
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2648 3844 4745 5007 820
Size: 197 926 705 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 705 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2004 2682 3179 513
Size: 552 939 678 497 3074
Fragments arranged by size:
3074 939 678 552 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2615 3986 5698
Size: 274 80 33 51 1443 638 1371 1712
Cuts at: 5698 96
Size: 138
Fragments arranged by size:
1712 1443 1371 638 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 2855 1316
Size: 1539 4201
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 2648 1943
Size: 705 5035
XmaI C'CCGG_G
Cuts at: 1870 2161 3065 1870
Size: 291 904 4545
Fragments arranged by size:
4545 904 291
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI