Restriction map for
pFN21AB0912
(Circular) (Six-base) MAPSORT of: pFN21AB0912.seq Check: 4233 from: 1 to: 5656
pFN21AB0912 5656 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2424 2424
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2471 3466 278
Size: 53 83 186 936 935 995 2468
Fragments arranged by size:
2468 995 936 935 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 2606 3719 5103 5371 1632
Size: 917 57 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 917 268 57
AfeI AGC'GCT
Cuts at: 2696 2696
Size: 5656
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2159 2226 4555 5532 1203
Size: 956 67 2329 977 1327
Fragments arranged by size:
2329 1327 977 956 67
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2319 2572 2779 3399 4971 2319
Size: 253 207 620 1572 3004
Fragments arranged by size:
3004 1572 620 253 207
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2977 4432 618
Size: 325 217 627 308 882 1455 1842
Fragments arranged by size:
1842 1455 882 627 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2975 1391
Size: 1584 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 928
Size: 971 572 4113
Fragments arranged by size:
4113 971 572
BbvCI CC'TCA_GC
Cuts at: 2312 2312
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2047 5651 2047
Size: 3604 2052
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 3717 4873 1790
Size: 1927 1156 2573
Fragments arranged by size:
2573 1927 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2131 2198 3387
Size: 416 53 83 399 24 714 67 1189
Cuts at: 3387 4345 442
Size: 958 1753
Fragments arranged by size:
1753 1189 958 714 416 399 83 67
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2480 2594 2663 4316 1239
Size: 1151 90 114 69 1653 2579
Fragments arranged by size:
2579 1653 1151 114 90 69
Bpu10I CC'TnA_GC
Cuts at: 2312 2312
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2830 3447 493
Size: 711 1626 617 2702
Fragments arranged by size:
2702 1626 711 617
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2468 3463 3845 4480
Size: 53 83 186 936 935 995 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 995 936 935 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 1178
Size: 30 165 30 1050 30 4351
Fragments arranged by size:
4351 1050 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2591 1186
Size: 910 495 4251
Fragments arranged by size:
4251 910 495
BseYI C'CCAG_C
Cuts at: 2643 4859 5355 2643
Size: 2216 496 2944
Fragments arranged by size:
2944 2216 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2190 3106 3867 4895 1540
Size: 275 186 189 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 189 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2679 3717 3802 4873 2328
Size: 351 1038 85 1071 3111
Fragments arranged by size:
3111 1071 1038 351 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2679 2975 3717 3802 4873 1391
Size: 399 538 351 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 351 296 85
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 2925 3545 5275 2925
Size: 620 1730 3306
Fragments arranged by size:
3306 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 3011 844
Size: 1580 587 3489
Fragments arranged by size:
3489 1580 587
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2756 3716 4728 2756
Size: 960 1012 3684
Fragments arranged by size:
3684 1012 960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2127 794
Size: 1333 4323
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2047 2598 2986 3353 3738 3755 5207 5293
Size: 75 551 388 367 385 17 1452 86
Cuts at: 5293 5305 5651 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 551 388 385 367 346 86
75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2434 487
Size: 683 56 1208 3709
Fragments arranged by size:
3709 1208 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2554 3172 3966 3986 2074
Size: 480 618 794 20 3744
Fragments arranged by size:
3744 794 618 480 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2139 2139
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2781 2832 3103
Size: 54 1425 50 275 18 951 51 271
Cuts at: 3103 3992 5621 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 951 889 275 271 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2943 3586 1353
Size: 1590 643 3423
Fragments arranged by size:
3423 1590 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 4243 4617 4763 387
Size: 1064 138 921 1733 374 146 1280
Fragments arranged by size:
1733 1280 1064 921 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2480 2549 2594 2606 2663 3719
Size: 393 758 90 69 45 12 57 1056
Cuts at: 3719 4316 5103 5371 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1056 787 758 597 393 268 90
69 57 45 12
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 2937 4162 2937
Size: 1225 4431
HaeII r_GCGC'y
Cuts at: 1678 2698 4803 1678
Size: 1020 2105 2531
Fragments arranged by size:
2531 2105 1020
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2640 2647 2672
Size: 32 135 32 881 32 155 7 25
Cuts at: 2672 2679 2701 2733 4297 4329 4371 4403 1373
Size: 7 22 32 1564 32 42 32 2626
Fragments arranged by size:
2626 1564 881 155 135 42 32 32
32 32 32 32 25 22 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 2554 2734 3000 3246 669
Size: 988 281 103 513 180 266 246 3079
Fragments arranged by size:
3079 988 513 281 266 246 180 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 2583 3255 2583
Size: 672 4984
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2783 2834 10
Size: 54 1425 343 951 51 2832
Fragments arranged by size:
2832 1425 951 343 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2474 2491 2518 2643 3072 3749 4897 5142
Size: 868 17 27 125 429 677 1148 245
Cuts at: 5142 1606
Size: 2120
Fragments arranged by size:
2120 1148 868 677 429 245 125 27
17
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2114 2163 2230 2536 3014 4559 1132
Size: 97 885 49 67 306 478 1545 2229
Fragments arranged by size:
2229 1545 885 478 306 97 67 49
PasI CC'CwG_GG
Cuts at: 1348 2773 1348
Size: 1425 4231
PciI A'CATG_T
Cuts at: 2159 2226 4555 2159
Size: 67 2329 3260
Fragments arranged by size:
3260 2329 67
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2779 2890 1108
Size: 1671 111 3874
Fragments arranged by size:
3874 1671 111
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 5624
PstI C_TGCA'G
Cuts at: 830 2244 3008 830
Size: 1414 764 3478
Fragments arranged by size:
3478 1414 764
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2474 2491 2518 2474
Size: 17 27 5612
Fragments arranged by size:
5612 27 17
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2240 2272 3004 4139 4820 5011 826
Size: 219 1195 32 732 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 732 681 219 191 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2919 1517
Size: 1402 4254
StyI C'CwwG_G
Cuts at: 513 1065 2004 3095 513
Size: 552 939 1091 3074
Fragments arranged by size:
3074 1091 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 2599 3683 3700 3853 4038 4483
Size: 1255 570 449 1084 17 153 185 445
Cuts at: 4483 325
Size: 1498
Fragments arranged by size:
1498 1255 1084 570 449 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2089 3902 5614
Size: 274 80 33 51 1443 112 1813 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1813 1712 1443 274 138 112 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2469 3464 276
Size: 53 83 186 936 935 995 2468
Fragments arranged by size:
2468 995 936 935 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI