Restriction map for
pFN21AB0869
(Circular) (Six-base) MAPSORT of: pFN21AB0869.seq Check: 6020 from: 1 to: 7927
pFN21AB0869 7927 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3497 5044 3497
Size: 1547 6380
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4379 5737 278
Size: 53 83 186 936 2843 1358 2468
Fragments arranged by size:
2843 2468 1358 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3225 5270 1937
Size: 1288 2045 4594
Fragments arranged by size:
4594 2045 1288
Acc65I G'GTAC_C
Cuts at: 3304 4989 5248 3304
Size: 1685 259 5983
Fragments arranged by size:
5983 1685 259
AclI AA'CG_TT
Cuts at: 1563 6054 6427 1563
Size: 4491 373 3063
Fragments arranged by size:
4491 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 3476 4136 5990 7374 7642 1632
Size: 592 1252 660 1854 1384 268 1917
Fragments arranged by size:
1917 1854 1384 1252 660 592 268
AfeI AGC'GCT
Cuts at: 2517 3249 3863 2517
Size: 732 614 6581
Fragments arranged by size:
6581 732 614
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7730
AflIII A'CryG_T
Cuts at: 1203 3963 4905 6826 7803 1203
Size: 2760 942 1921 977 1327
Fragments arranged by size:
2760 1921 1327 977 942
AgeI A'CCGG_T
Cuts at: 2382 6758 2382
Size: 4376 3551
AhdI GACnn_n'nnGTC
Cuts at: 6656 6656
Size: 7927
AleI CACnn'nnGTG
Cuts at: 1166 2933 3633 1166
Size: 1767 700 5460
Fragments arranged by size:
5460 1767 700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3350 4526 1184
Size: 2166 1176 4585
Fragments arranged by size:
4585 2166 1176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2092 2124 4546 4578 2092
Size: 32 2422 32 5441
Fragments arranged by size:
5441 2422 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3094 3397 3489 4041 4290 5670 7242 3094
Size: 303 92 552 249 1380 1572 3779
Fragments arranged by size:
3779 1572 1380 552 303 249 92
ApaI G_GGCC'C
Cuts at: 3006 3502 3758 3006
Size: 496 256 7175
Fragments arranged by size:
7175 496 256
ApaLI G'TGCA_C
Cuts at: 5984 7140 5984
Size: 1156 6771
ApoI r'AATT_y
Cuts at: 1493 2683 4225 5236 5462 5614 7723 1493
Size: 1190 1542 1011 226 152 2109 1697
Fragments arranged by size:
2109 1697 1542 1190 1011 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6481 160
Size: 872 5449 1606
Fragments arranged by size:
5449 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7927
AvaI C'yCGr_G
Cuts at: 1870 1943 2757 5252 1870
Size: 73 814 2495 4545
Fragments arranged by size:
4545 2495 814 73
AvrII C'CTAG_G
Cuts at: 2596 2596
Size: 7927
BamHI G'GATC_C
Cuts at: 3686 5257 5624 3686
Size: 1571 367 5989
Fragments arranged by size:
5989 1571 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3077 3304 3462 4280 4989
Size: 325 217 627 1290 227 158 818 709
Cuts at: 4989 5248 6703 618
Size: 259 1455 1842
Fragments arranged by size:
1842 1455 1290 818 709 627 325 259
227 217 158
BanII G_rGCy'C
Cuts at: 1391 2541 3006 3354 3378 3502 3758 5246 1391
Size: 1150 465 348 24 124 256 1488 4072
Fragments arranged by size:
4072 1488 1150 465 348 256 124 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2805 2959 4127 4249 5183 928
Size: 971 906 154 1168 122 934 3672
Fragments arranged by size:
3672 1168 971 934 906 154 122
BbvCI CC'TCA_GC
Cuts at: 2953 2953
Size: 7927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 4182 4216 6118 6152 1903
Size: 34 1814 34 397 34 1902 34 3678
Fragments arranged by size:
3678 1902 1814 397 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3057 5819 7029 3057
Size: 2762 1210 3955
Fragments arranged by size:
3955 2762 1210
BclI T'GATC_A
Cuts at: 1548 2461 1548
Size: 913 7014
BfrBI ATG'CAT
Cuts at: 2408 3151 2408
Size: 743 7184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4214 5108 6538
Size: 107 122 71 631 605 2542 894 1430
Cuts at: 6538 136
Size: 1525
Fragments arranged by size:
2542 1525 1430 894 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5203 7922 5203
Size: 2719 5208
BlpI GC'TnA_GC
Cuts at: 4931 5344 4931
Size: 413 7514
Bme1580I G_kGCm'C
Cuts at: 1790 3006 3183 3502 3758 5988 7144 1790
Size: 1216 177 319 256 2230 1156 2573
Fragments arranged by size:
2573 2230 1216 1156 319 256 177
BmgBI CAC'GTC
Cuts at: 1318 2573 3229 1318
Size: 1255 656 6016
Fragments arranged by size:
6016 1255 656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4514 5181 5658
Size: 416 53 83 399 24 3097 667 477
Cuts at: 5658 6616 442
Size: 958 1753
Fragments arranged by size:
3097 1753 958 667 477 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2731 3572 6587 1239
Size: 1492 841 3015 2579
Fragments arranged by size:
3015 2579 1492 841
Bpu10I CC'TnA_GC
Cuts at: 2898 2953 2975 3563 4110 2898
Size: 55 22 588 547 6715
Fragments arranged by size:
6715 588 547 55 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2273 3307 6052 6917 7215 2023
Size: 250 1034 2745 865 298 2735
Fragments arranged by size:
2745 2735 1034 865 298 250
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3648 3822 6590 882
Size: 617 2149 174 2768 2219
Fragments arranged by size:
2768 2219 2149 617 174
BsaAI yAC'GTr
Cuts at: 493 1204 3100 3217 3439 5718 493
Size: 711 1896 117 222 2279 2702
Fragments arranged by size:
2702 2279 1896 711 222 117
BsaBI GATnn'nnATC
Cuts at: 7921 7921
Size: 7927
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3653 4376 4740 5734
Size: 53 83 186 936 2120 723 364 994
Cuts at: 5734 6116 6751 275
Size: 382 635 1451
Fragments arranged by size:
2120 1451 994 936 723 635 382 364
186 83 53
BsaWI w'CCGG_w
Cuts at: 2382 4422 6360 6758 7032 7179 7590 2382
Size: 2040 1938 398 274 147 411 2719
Fragments arranged by size:
2719 2040 1938 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2598 2628 4070 4100 1178
Size: 30 165 30 1195 30 1442 30 5005
Fragments arranged by size:
5005 1442 1195 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2601 3569 4946 1186
Size: 1324 91 968 1377 4167
Fragments arranged by size:
4167 1377 1324 968 91
BseYI C'CCAG_C
Cuts at: 2499 3519 3978 4328 7130 7626 2499
Size: 1020 459 350 2802 496 2800
Fragments arranged by size:
2802 2800 1020 496 459 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2407 3254 3383 4083 4411 4637 1163
Size: 573 671 847 129 700 328 226 4453
Fragments arranged by size:
4453 847 700 671 573 328 226 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2383 4820 5377 6138 7166 1540
Size: 275 186 382 2437 557 761 1028 2301
Fragments arranged by size:
2437 2301 1028 761 557 382 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3354 3438 4796 5988 6073 7144 2541
Size: 813 84 1358 1192 85 1071 3324
Fragments arranged by size:
3324 1358 1192 1071 813 85 84
BsmI GAATG_Cn'
Cuts at: 2170 2408 4020 4413 4470 4505 5438 5531 2170
Size: 238 1612 393 57 35 933 93 4566
Fragments arranged by size:
4566 1612 933 393 238 93 57 35
BsmBI CGTCTCn'nnnn_
Cuts at: 5035 5035
Size: 7927
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2541 3006 3183 3354 3378 3438 3502
Size: 399 751 465 177 171 24 60 64
Cuts at: 3502 3758 4796 5246 5988 6073 7144 1391
Size: 256 1038 450 742 85 1071 2174
Fragments arranged by size:
2174 1071 1038 751 742 465 450 399
256 177 171 85 64 60 24
BspEI T'CCGG_A
Cuts at: 7590 7590
Size: 7927
BspHI T'CATG_A
Cuts at: 2817 5816 7546 2817
Size: 2999 1730 3198
Fragments arranged by size:
3198 2999 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 5044 5282 844
Size: 2653 1547 238 3489
Fragments arranged by size:
3489 2653 1547 238
BsrBI CCG'CTC
Cuts at: 1394 1823 2433 3708 4602 5814 1394
Size: 429 610 1275 894 1212 3507
Fragments arranged by size:
3507 1275 1212 894 610 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 3943 4078 6422 6596 65
Size: 1183 1695 1000 135 2344 174 1396
Fragments arranged by size:
2344 1695 1396 1183 1000 174 135
BsrFI r'CCGG_y
Cuts at: 1700 1810 2382 4815 6571 6758 1700
Size: 110 572 2433 1756 187 2869
Fragments arranged by size:
2869 2433 1756 572 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7927
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7927
BssSI C'ACGA_G
Cuts at: 3811 5987 6999 3811
Size: 2176 1012 4739
Fragments arranged by size:
4739 2176 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3397 3489 4791 794
Size: 2603 92 1302 3930
Fragments arranged by size:
3930 2603 1302 92
BstBI TT'CG_AA
Cuts at: 6731 6731
Size: 7927
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7927
BstXI CCAn_nnnn'nTGG
Cuts at: 4698 4698
Size: 7927
BstYI r'GATC_y
Cuts at: 1972 2257 2454 3393 3447 3686 4038 4865 5203
Size: 285 197 939 54 239 352 827 338
Cuts at: 5203 5257 5624 6009 6026 7478 7564 7576 7922
Size: 54 367 385 17 1452 86 12 346
Cuts at: 7922 1972
Size: 1977
Fragments arranged by size:
1977 1452 939 827 385 367 352 346
338 285 239 197 86 54 54 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2556 3081 3618 3647 3681 4779 4806
Size: 552 1491 525 537 29 34 1098 27
Cuts at: 4806 4923 513
Size: 117 3517
Fragments arranged by size:
3517 1491 1098 552 537 525 117 34
29 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2968 487
Size: 683 56 1742 5446
Fragments arranged by size:
5446 1742 683 56
BtsI GCAGTG_nn'
Cuts at: 3581 3737 3752 5443 6237 6257 3581
Size: 156 15 1691 794 20 5251
Fragments arranged by size:
5251 1691 794 156 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7927
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7892
DraI TTT'AAA
Cuts at: 5231 5231
Size: 7927
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3814 4381 6934 809
Size: 507 2498 567 2553 1802
Fragments arranged by size:
2553 2498 1802 567 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3270 4171 4817
Size: 54 1425 50 275 18 1440 901 646
Cuts at: 4817 5374 6263 7892 8
Size: 557 889 1629 43
Fragments arranged by size:
1629 1440 1425 901 889 646 557 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4817 5374 1537
Size: 275 3005 557 4090
Fragments arranged by size:
4090 3005 557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 3336 3359 3452 4656 4875 5857 1353
Size: 890 1093 23 93 1204 219 982 3423
Fragments arranged by size:
3423 1204 1093 982 890 219 93 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3382 6514 6888 7034 387
Size: 1064 138 1793 3132 374 146 1280
Fragments arranged by size:
3132 1793 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 3352 2539
Size: 813 7114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2731 3476 3572 4136 5990 6587
Size: 393 592 507 745 96 564 1854 597
Cuts at: 6587 7374 7642 1239
Size: 787 268 1524
Fragments arranged by size:
1854 1524 787 745 597 592 564 507
393 268 96
EcoO109I rG'GnC_Cy
Cuts at: 3003 3091 3498 3505 3754 3755 3983 4596 3003
Size: 88 407 7 249 1 228 613 6334
Fragments arranged by size:
6334 613 407 249 228 88 7 1
EcoRI G'AATT_C
Cuts at: 5236 5614 5236
Size: 378 7549
FspI TGC'GCA
Cuts at: 6433 6433
Size: 7927
HaeII r_GCGC'y
Cuts at: 1678 2519 2788 2982 3251 3681 3865 7074 1678
Size: 841 269 194 269 430 184 3209 2531
Fragments arranged by size:
3209 2531 841 430 269 269 194 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2446 2478 3595
Size: 32 135 32 674 32 168 32 1117
Cuts at: 3595 3627 3678 3710 4070 4102 4236 4268 4623
Size: 32 51 32 360 32 134 32 355
Cuts at: 4623 4655 4685 4717 6568 6600 6642 6674 1373
Size: 32 30 32 1851 32 42 32 2626
Fragments arranged by size:
2626 1851 1117 674 360 355 168 135
134 51 42 32 32 32 32 32
32 32 32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 2187 3226 4153 4354 5271 5517
Size: 988 281 249 1039 927 201 917 246
Cuts at: 5517 669
Size: 3079
Fragments arranged by size:
3079 1039 988 927 917 281 249 246
201
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7927
HpaI GTT'AAC
Cuts at: 2187 5517 2187
Size: 3330 4597
KpnI G_GTAC'C
Cuts at: 3308 4993 5252 3308
Size: 1685 259 5983
Fragments arranged by size:
5983 1685 259
MfeI C'AATT_G
Cuts at: 5526 5526
Size: 7927
MluI A'CGCG_T
Cuts at: 7803 7803
Size: 7927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3626 4683 4692 7041 7225 1305
Size: 2321 1057 9 2349 184 2007
Fragments arranged by size:
2349 2321 2007 1057 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3272 10
Size: 54 1425 343 1440 4665
Fragments arranged by size:
4665 1440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2727 2816 2933 3146 3633 3669
Size: 648 1194 367 89 117 213 487 36
Cuts at: 3669 5885 6244 6403 518
Size: 2216 359 159 2042
Fragments arranged by size:
2216 2042 1194 648 487 367 359 213
159 117 89 36
MspA1I CmG'CkG
Cuts at: 1606 2484 2528 2957 3330 4987 5343 6020 7168
Size: 878 44 429 373 1657 356 677 1148
Cuts at: 7168 7413 1606
Size: 245 2120
Fragments arranged by size:
2120 1657 1148 878 677 429 373 356
245 44
NaeI GCC'GGC
Cuts at: 4817 4817
Size: 7927
NcoI C'CATG_G
Cuts at: 513 1065 2556 4779 513
Size: 552 1491 2223 3661
Fragments arranged by size:
3661 2223 1491 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7927
NgoMIV G'CCGG_C
Cuts at: 4815 4815
Size: 7927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7927
NotI GC'GGCC_GC
Cuts at: 5374 5374
Size: 7927
NsiI A_TGCA'T
Cuts at: 2410 3153 2410
Size: 743 7184
NspI r_CATG'y
Cuts at: 1132 1229 3967 4203 4575 4909 5285 6830 1132
Size: 97 2738 236 372 334 376 1545 2229
Fragments arranged by size:
2738 2229 1545 376 372 334 236 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 7198
PciI A'CATG_T
Cuts at: 3963 4905 6826 3963
Size: 942 1921 5064
Fragments arranged by size:
5064 1921 942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2278 4131 4526 1108
Size: 1170 1853 395 4509
Fragments arranged by size:
4509 1853 1170 395
PfoI T'CCnGG_A
Cuts at: 2566 3782 4796 2566
Size: 1216 1014 5697
Fragments arranged by size:
5697 1216 1014
PmeI GTTT'AAAC
Cuts at: 5231 5231
Size: 7927
PmlI CAC'GTG
Cuts at: 3217 3439 3217
Size: 222 7705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5996 6028 7509 7541 5996
Size: 32 1481 32 6382
Fragments arranged by size:
6382 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3505 3983 4596 3505
Size: 478 613 6836
Fragments arranged by size:
6836 613 478
PshAI GACnn'nnGTC
Cuts at: 1613 4148 1613
Size: 2535 5392
PsiI TTA'TAA
Cuts at: 5497 5497
Size: 7927
PspOMI G'GGCC_C
Cuts at: 3002 3498 3754 3002
Size: 496 256 7175
Fragments arranged by size:
7175 496 256
PstI C_TGCA'G
Cuts at: 830 3126 3393 3827 3872 4406 5279 830
Size: 2296 267 434 45 534 873 3478
Fragments arranged by size:
3478 2296 873 534 434 267 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7927
PvuII CAG'CTG
Cuts at: 2528 2957 3330 2528
Size: 429 373 7125
Fragments arranged by size:
7125 429 373
SacI G_AGCT'C
Cuts at: 2541 3354 2541
Size: 813 7114
SalI G'TCGA_C
Cuts at: 1936 3224 5269 1936
Size: 1288 2045 4594
Fragments arranged by size:
4594 2045 1288
SapI GCTCTTCn'nnn_
Cuts at: 3359 3359
Size: 7927
SbfI CC_TGCA'GG
Cuts at: 5279 5279
Size: 7927
ScaI AGT'ACT
Cuts at: 2884 6175 7887 2884
Size: 3291 1712 2924
Fragments arranged by size:
3291 2924 1712
SfcI C'TryA_G
Cuts at: 826 1045 2491 2550 3122 3389 3823 3868 4402
Size: 219 1446 59 572 267 434 45 534
Cuts at: 4402 5275 6410 7091 7282 826
Size: 873 1135 681 191 1471
Fragments arranged by size:
1471 1446 1135 873 681 572 534 434
267 219 191 59 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7927
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7927
SmaI CCC'GGG
Cuts at: 1872 2759 5254 1872
Size: 887 2495 4545
Fragments arranged by size:
4545 2495 887
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 2252 3322 6031 6932 7194
Size: 197 926 95 214 1070 2709 901 262
Cuts at: 7194 820
Size: 1553
Fragments arranged by size:
2709 1553 1070 926 901 262 214 197
95
SnaBI TAC'GTA
Cuts at: 493 5718 493
Size: 5225 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7927
SphI G_CATG'C
Cuts at: 1132 5285 1132
Size: 4153 3774
SspI AAT'ATT
Cuts at: 5 52 5851 5
Size: 47 5799 2081
Fragments arranged by size:
5799 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 2993 1517
Size: 795 681 6451
Fragments arranged by size:
6451 795 681
StyI C'CwwG_G
Cuts at: 513 1065 2556 2596 3986 4474 4779 5366 513
Size: 552 1491 40 1390 488 305 587 3074
Fragments arranged by size:
3074 1491 1390 587 552 488 305 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2082 2363 3125 5954 5971 6124 6309
Size: 1255 502 281 762 2829 17 153 185
Cuts at: 6309 6754 325
Size: 445 1498
Fragments arranged by size:
2829 1498 1255 762 502 445 281 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 2882 6173
Size: 274 80 33 51 1443 651 254 3291
Cuts at: 6173 7885 96
Size: 1712 138
Fragments arranged by size:
3291 1712 1443 651 274 254 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 4517 4549 7887 7919 2273
Size: 32 2212 32 3338 32 2281
Fragments arranged by size:
3338 2281 2212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7927
XbaI T'CTAG_A
Cuts at: 5263 5263
Size: 7927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3071 3966 4013 3071
Size: 895 47 6985
Fragments arranged by size:
6985 895 47
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7927
XmaI C'CCGG_G
Cuts at: 1870 2757 5252 1870
Size: 887 2495 4545
Fragments arranged by size:
4545 2495 887
XmnI GAAnn'nnTTC
Cuts at: 6056 6056
Size: 7927
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4377 5735 276
Size: 53 83 186 936 2843 1358 2468
Fragments arranged by size:
2843 2468 1358 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI