Restriction map for
pFN21AB0668
(Circular) (Six-base) MAPSORT of: pFN21AB0668.seq Check: 284 from: 1 to: 7705
pFN21AB0668 7705 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5515 278
Size: 53 83 186 936 3979 2468
Fragments arranged by size:
3979 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5048 1937
Size: 3111 4594
Acc65I G'GTAC_C
Cuts at: 2737 5026 2737
Size: 2289 5416
AclI AA'CG_TT
Cuts at: 1563 5832 6205 1563
Size: 4269 373 3063
Fragments arranged by size:
4269 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2694 3903 4073 4889 4953 5768 7152 7420
Size: 1062 1209 170 816 64 815 1384 268
Cuts at: 7420 1632
Size: 1917
Fragments arranged by size:
1917 1384 1209 1062 816 815 268 170
64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7508
AflIII A'CryG_T
Cuts at: 1203 6604 7581 1203
Size: 5401 977 1327
Fragments arranged by size:
5401 1327 977
AgeI A'CCGG_T
Cuts at: 2698 6536 2698
Size: 3838 3867
AhdI GACnn_n'nnGTC
Cuts at: 3781 6434 3781
Size: 2653 5052
AleI CACnn'nnGTG
Cuts at: 1166 3539 1166
Size: 2373 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2757 1184
Size: 1573 6132
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2646 2678 2646
Size: 32 7673
AlwNI CAG_nnn'CTG
Cuts at: 5448 7020 5448
Size: 1572 6133
ApaLI G'TGCA_C
Cuts at: 5762 6918 5762
Size: 1156 6549
ApoI r'AATT_y
Cuts at: 1493 2461 2526 3306 3802 4962 5014 5240 5392
Size: 968 65 780 496 1160 52 226 152
Cuts at: 5392 7501 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1160 968 780 496 226 152
65 52
AseI AT'TA_AT
Cuts at: 160 1032 6259 160
Size: 872 5227 1606
Fragments arranged by size:
5227 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7705
AvaI C'yCGr_G
Cuts at: 1870 1943 5030 1870
Size: 73 3087 4545
Fragments arranged by size:
4545 3087 73
BamHI G'GATC_C
Cuts at: 2378 5035 5402 2378
Size: 2657 367 4681
Fragments arranged by size:
4681 2657 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2737 3141 3239 4307
Size: 325 217 627 252 698 404 98 1068
Cuts at: 4307 5026 6481 618
Size: 719 1455 1842
Fragments arranged by size:
1842 1455 1068 719 698 627 404 325
252 217 98
BanII G_rGCy'C
Cuts at: 1391 2758 5024 1391
Size: 1367 2266 4072
Fragments arranged by size:
4072 2266 1367
BbeI G_GCGC'C
Cuts at: 2043 4311 2043
Size: 2268 5437
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4202 4263 4677 928
Size: 971 2303 61 414 3956
Fragments arranged by size:
3956 2303 971 414 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5896 5930 1903
Size: 34 3959 34 3678
Fragments arranged by size:
3959 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2534 4059 5597 6807 2534
Size: 1525 1538 1210 3432
Fragments arranged by size:
3432 1538 1525 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7705
BfrBI ATG'CAT
Cuts at: 2399 2399
Size: 7705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6316 136
Size: 107 122 71 631 605 4644 1525
Fragments arranged by size:
4644 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7700 7700
Size: 7705
BlpI GC'TnA_GC
Cuts at: 5122 5122
Size: 7705
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2112 3242 3818 4192 5766 6922 1790
Size: 273 49 1130 576 374 1574 1156 2573
Fragments arranged by size:
2573 1574 1156 1130 576 374 273 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7705
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 3010 4687
Size: 416 53 83 399 24 697 896 1677
Cuts at: 4687 5436 6394 442
Size: 749 958 1753
Fragments arranged by size:
1753 1677 958 896 749 697 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7705
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4609 4611 4609
Size: 2 7703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2667 6365 1239
Size: 1428 3698 2579
Fragments arranged by size:
3698 2579 1428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3312 5830 6695 6993 3312
Size: 2518 865 298 4024
Fragments arranged by size:
4024 2518 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4380 6368 882
Size: 617 2881 1988 2219
Fragments arranged by size:
2881 2219 1988 617
BsaAI yAC'GTr
Cuts at: 493 1204 5496 493
Size: 711 4292 2702
Fragments arranged by size:
4292 2702 711
BsaBI GATnn'nnATC
Cuts at: 7699 7699
Size: 7705
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3531 4308 5512
Size: 53 83 186 936 507 1491 777 1204
Cuts at: 5512 5894 6529 275
Size: 382 635 1451
Fragments arranged by size:
1491 1451 1204 936 777 635 507 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2698 2923 6138 6536 6810 6957 7368 2698
Size: 225 3215 398 274 147 411 3035
Fragments arranged by size:
3215 3035 411 398 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7480
Fragments arranged by size:
7480 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 2071 2204 3023 3098 3208 4419 1186
Size: 882 3 133 819 75 110 1211 4472
Fragments arranged by size:
4472 1211 882 819 133 110 75 3
BseYI C'CCAG_C
Cuts at: 2601 4998 6908 7404 2601
Size: 2397 1910 496 2902
Fragments arranged by size:
2902 2397 1910 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2735 1163
Size: 573 999 6133
Fragments arranged by size:
6133 999 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5155 5916 6944 1540
Size: 275 186 3154 761 1028 2301
Fragments arranged by size:
3154 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2437 4678 5766 5851 6922 2437
Size: 2241 1088 85 1071 3220
Fragments arranged by size:
3220 2241 1088 1071 85
BsmI GAATG_Cn'
Cuts at: 2401 5216 5309 2401
Size: 2815 93 4797
Fragments arranged by size:
4797 2815 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3019 3019
Size: 7705
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2112 2437 2758 3242 3818 4192
Size: 399 273 49 325 321 484 576 374
Cuts at: 4192 4678 5024 5766 5851 6922 1391
Size: 486 346 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 576 486 484 399 374
346 325 321 273 85 49
BspEI T'CCGG_A
Cuts at: 2923 7368 2923
Size: 4445 3260
BspHI T'CATG_A
Cuts at: 5594 7324 5594
Size: 1730 5975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2725 2749 5060 844
Size: 1881 24 2311 3489
Fragments arranged by size:
3489 2311 1881 24
BsrBI CCG'CTC
Cuts at: 1394 1823 5592 1394
Size: 429 3769 3507
Fragments arranged by size:
3769 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2589 6200 6374 65
Size: 1183 1341 3611 174 1396
Fragments arranged by size:
3611 1396 1341 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2698 2722 6349 6536 1700
Size: 110 888 24 3627 187 2869
Fragments arranged by size:
3627 2869 888 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 4037 96
Size: 3941 3764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7705
BssSI C'ACGA_G
Cuts at: 5765 6777 5765
Size: 1012 6693
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7705
BstBI TT'CG_AA
Cuts at: 2417 6509 2417
Size: 4092 3613
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7705
BstXI CCAn_nnnn'nTGG
Cuts at: 2564 3234 3507 3541 2564
Size: 670 273 34 6728
Fragments arranged by size:
6728 670 273 34
BstYI r'GATC_y
Cuts at: 1972 2378 2687 2920 4604 5035 5402 5787 5804
Size: 406 309 233 1684 431 367 385 17
Cuts at: 5804 7256 7342 7354 7700 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1684 1452 431 406 385 367 346
309 233 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 513
Size: 552 939 84 6130
Fragments arranged by size:
6130 939 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6966
Fragments arranged by size:
6966 683 56
BtsI GCAGTG_nn'
Cuts at: 2278 2618 4158 5221 6015 6035 2278
Size: 340 1540 1063 794 20 3948
Fragments arranged by size:
3948 1540 1063 794 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7705
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2164 2199 529
Size: 35 1600 35 6035
Fragments arranged by size:
6035 1600 35 35
DraI TTT'AAA
Cuts at: 5009 5009
Size: 7705
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6712 809
Size: 507 5396 1802
Fragments arranged by size:
5396 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2085 4158
Size: 54 1425 50 275 18 177 78 2073
Cuts at: 4158 4719 5152 6041 7670 8
Size: 561 433 889 1629 43
Fragments arranged by size:
2073 1629 1425 889 561 433 275 177
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5152 1537
Size: 275 3340 4090
Fragments arranged by size:
4090 3340 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 3879 4908 5635 1353
Size: 1647 879 1029 727 3423
Fragments arranged by size:
3423 1647 1029 879 727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6292 6666 6812 387
Size: 1064 138 4703 374 146 1280
Fragments arranged by size:
4703 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2667 2694 3903 4073 4889 4953 5768
Size: 393 1035 27 1209 170 816 64 815
Cuts at: 5768 6365 7152 7420 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1209 1035 816 815 787 597 393
268 170 64 27
EcoO109I rG'GnC_Cy
Cuts at: 2627 2627
Size: 7705
EcoRI G'AATT_C
Cuts at: 2526 5014 5392 2526
Size: 2488 378 4839
Fragments arranged by size:
4839 2488 378
FspI TGC'GCA
Cuts at: 6211 6211
Size: 7705
HaeII r_GCGC'y
Cuts at: 1678 2043 2959 4311 6852 1678
Size: 365 916 1352 2541 2531
Fragments arranged by size:
2541 2531 1352 916 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3670 3702 3767 3799 4007
Size: 32 135 32 2098 32 65 32 208
Cuts at: 4007 4039 6346 6378 6420 6452 1373
Size: 32 2307 32 42 32 2626
Fragments arranged by size:
2626 2307 2098 208 135 65 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3334 5049 5295 669
Size: 988 281 1396 1715 246 3079
Fragments arranged by size:
3079 1715 1396 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7705
HpaI GTT'AAC
Cuts at: 5295 5295
Size: 7705
KasI G'GCGC_C
Cuts at: 2039 4307 2039
Size: 2268 5437
KpnI G_GTAC'C
Cuts at: 2741 5030 2741
Size: 2289 5416
MfeI C'AATT_G
Cuts at: 3174 3654 4380 5304 3174
Size: 480 726 924 5575
Fragments arranged by size:
5575 924 726 480
MluI A'CGCG_T
Cuts at: 7581 7581
Size: 7705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2308 3689 4801 6819 7003 1305
Size: 1003 1381 1112 2018 184 2007
Fragments arranged by size:
2018 2007 1381 1112 1003 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4721 10
Size: 54 1425 343 2889 2994
Fragments arranged by size:
2994 2889 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3690 5663 6022 6181 518
Size: 648 2373 151 1973 359 159 2042
Fragments arranged by size:
2373 2042 1973 648 359 159 151
MspA1I CmG'CkG
Cuts at: 1606 2034 2605 5121 5798 6946 7191 1606
Size: 428 571 2516 677 1148 245 2120
Fragments arranged by size:
2516 2120 1148 677 571 428 245
NarI GG'CG_CC
Cuts at: 2040 4308 2040
Size: 2268 5437
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6214
Fragments arranged by size:
6214 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7705
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7705
NotI GC'GGCC_GC
Cuts at: 5152 5152
Size: 7705
NsiI A_TGCA'T
Cuts at: 2401 2401
Size: 7705
NspI r_CATG'y
Cuts at: 1132 1229 3695 5006 5063 6608 1132
Size: 97 2466 1311 57 1545 2229
Fragments arranged by size:
2466 2229 1545 1311 97 57
PasI CC'CwG_GG
Cuts at: 1348 3267 1348
Size: 1919 5786
PciI A'CATG_T
Cuts at: 6604 6604
Size: 7705
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2651 3578 1108
Size: 1543 927 5235
Fragments arranged by size:
5235 1543 927
PfoI T'CCnGG_A
Cuts at: 2683 2683
Size: 7705
PmeI GTTT'AAAC
Cuts at: 5009 5009
Size: 7705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5774 5806 7287 7319 5774
Size: 32 1481 32 6160
Fragments arranged by size:
6160 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7705
PsiI TTA'TAA
Cuts at: 3630 5275 3630
Size: 1645 6060
PstI C_TGCA'G
Cuts at: 830 2317 2610 5057 830
Size: 1487 293 2447 3478
Fragments arranged by size:
3478 2447 1487 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7705
PvuII CAG'CTG
Cuts at: 2605 2605
Size: 7705
SalI G'TCGA_C
Cuts at: 1936 5047 1936
Size: 3111 4594
SapI GCTCTTCn'nnn_
Cuts at: 3879 4908 3879
Size: 1029 6676
SbfI CC_TGCA'GG
Cuts at: 5057 5057
Size: 7705
ScaI AGT'ACT
Cuts at: 5953 7665 5953
Size: 1712 5993
SexAI A'CCwGG_T
Cuts at: 2428 2428
Size: 7705
SfcI C'TryA_G
Cuts at: 826 1045 2313 2606 5053 6188 6869 7060 826
Size: 219 1268 293 2447 1135 681 191 1471
Fragments arranged by size:
2447 1471 1268 1135 681 293 219 191
SfoI GGC'GCC
Cuts at: 2041 4309 2041
Size: 2268 5437
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7705
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7705
SmaI CCC'GGG
Cuts at: 1872 5032 1872
Size: 3160 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3327 5809 6710 6972 820
Size: 197 926 1384 2482 901 262 1553
Fragments arranged by size:
2482 1553 1384 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5496 493
Size: 5003 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7705
SphI G_CATG'C
Cuts at: 1132 3695 5063 1132
Size: 2563 1368 3774
Fragments arranged by size:
3774 2563 1368
SspI AAT'ATT
Cuts at: 5 52 2290 3553 3768 3986 4462 5629 5
Size: 47 2238 1263 215 218 476 1167 2081
Fragments arranged by size:
2238 2081 1263 1167 476 218 215 47
StuI AGG'CCT
Cuts at: 1517 2132 1517
Size: 615 7090
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 2521 4072 4368 4794 5144
Size: 552 939 199 318 1551 296 426 350
Cuts at: 5144 513
Size: 3074
Fragments arranged by size:
3074 1551 939 552 426 350 318 296
199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 2994 3512 5732 5749 5902 6087
Size: 1255 561 853 518 2220 17 153 185
Cuts at: 6087 6532 325
Size: 445 1498
Fragments arranged by size:
2220 1498 1255 853 561 518 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3032 4037 5951
Size: 274 80 33 51 1443 1055 1005 1914
Cuts at: 5951 7663 96
Size: 1712 138
Fragments arranged by size:
1914 1712 1443 1055 1005 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7665 7697 7665
Size: 32 7673
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7705
XbaI T'CTAG_A
Cuts at: 5041 5041
Size: 7705
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7705
XmaI C'CCGG_G
Cuts at: 1870 5030 1870
Size: 3160 4545
XmnI GAAnn'nnTTC
Cuts at: 2461 5834 2461
Size: 3373 4332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5513 276
Size: 53 83 186 936 3979 2468
Fragments arranged by size:
3979 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI