Restriction map for
pFN21AB0499
(Circular) (Six-base) MAPSORT of: pFN21AB0499.seq Check: 1609 from: 1 to: 6967
pFN21AB0499 6967 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2512 4777 278
Size: 53 83 186 936 976 2265 2468
Fragments arranged by size:
2468 2265 976 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 4310 1937
Size: 1452 921 4594
Fragments arranged by size:
4594 1452 921
Acc65I G'GTAC_C
Cuts at: 2228 4288 2228
Size: 2060 4907
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2943 3917 5030 6414 6682 1632
Size: 1311 974 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1113 974 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6770
AflIII A'CryG_T
Cuts at: 1203 2807 5866 6843 1203
Size: 1604 3059 977 1327
Fragments arranged by size:
3059 1604 1327 977
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 5696 5696
Size: 6967
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6967
AlwNI CAG_nnn'CTG
Cuts at: 4710 6282 4710
Size: 1572 5395
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 6967
ApaLI G'TGCA_C
Cuts at: 2493 5024 6180 2493
Size: 2531 1156 3280
Fragments arranged by size:
3280 2531 1156
ApoI r'AATT_y
Cuts at: 1493 2318 2540 3179 3604 3977 4054 4064 4276
Size: 825 222 639 425 373 77 10 212
Cuts at: 4276 4502 4654 6763 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 639 425 373 226 222
212 152 77 10
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 4292 1870
Size: 73 2349 4545
Fragments arranged by size:
4545 2349 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2220 2253 2220
Size: 33 6934
BamHI G'GATC_C
Cuts at: 4297 4664 4297
Size: 367 6600
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 4288 5743 618
Size: 325 217 627 441 2060 1455 1842
Fragments arranged by size:
2060 1842 1455 627 441 325 217
BanII G_rGCy'C
Cuts at: 1391 2033 4286 1391
Size: 642 2253 4072
Fragments arranged by size:
4072 2253 642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3423 3894 928
Size: 971 1524 471 4001
Fragments arranged by size:
4001 1524 971 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5158 5192 1903
Size: 34 3221 34 3678
Fragments arranged by size:
3678 3221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4859 6069 4859
Size: 1210 5757
BclI T'GATC_A
Cuts at: 1548 2116 1548
Size: 568 6399
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5578 136
Size: 107 122 71 631 605 3906 1525
Fragments arranged by size:
3906 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4123 6962 4123
Size: 2839 4128
BlpI GC'TnA_GC
Cuts at: 4384 4384
Size: 6967
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2497 5028 6184 1790
Size: 243 464 2531 1156 2573
Fragments arranged by size:
2573 2531 1156 464 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2985 3068 4698
Size: 416 53 83 399 24 1568 83 1630
Cuts at: 4698 5656 442
Size: 958 1753
Fragments arranged by size:
1753 1630 1568 958 416 399 83 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3000 4107 5627 1239
Size: 1761 1107 1520 2579
Fragments arranged by size:
2579 1761 1520 1107
Bpu10I CC'TnA_GC
Cuts at: 4172 4172
Size: 6967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3834 3866 5092 5957 6255 3834
Size: 32 1226 865 298 4546
Fragments arranged by size:
4546 1226 865 298 32
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5630 882
Size: 617 4131 2219
Fragments arranged by size:
4131 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3094 4758 493
Size: 711 1890 1664 2702
Fragments arranged by size:
2702 1890 1664 711
BsaBI GATnn'nnATC
Cuts at: 3278 6961 3278
Size: 3683 3284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2509 4774 5156 5791
Size: 53 83 186 936 976 2265 382 635
Cuts at: 5791 275
Size: 1451
Fragments arranged by size:
2265 1451 976 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5400 5798 6072 6219 6630 5400
Size: 398 274 147 411 5737
Fragments arranged by size:
5737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2998 3028 1178
Size: 30 165 30 1595 30 5117
Fragments arranged by size:
5117 1595 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2055 1186
Size: 869 6098
BseYI C'CCAG_C
Cuts at: 6170 6666 6170
Size: 496 6471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6394
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 5178 6206 1540
Size: 275 186 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2497 5028 5113 6184 2497
Size: 2531 85 1071 3280
Fragments arranged by size:
3280 2531 1071 85
BsmI GAATG_Cn'
Cuts at: 2447 2701 3192 4478 4571 2447
Size: 254 491 1286 93 4843
Fragments arranged by size:
4843 1286 491 254 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2497 4286 5028 5113 6184 1391
Size: 399 243 464 1789 742 85 1071 2174
Fragments arranged by size:
2174 1789 1071 742 464 399 243 85
BspEI T'CCGG_A
Cuts at: 6630 6630
Size: 6967
BspHI T'CATG_A
Cuts at: 2952 4856 6586 2952
Size: 1904 1730 3333
Fragments arranged by size:
3333 1904 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3496 4322 844
Size: 2652 826 3489
Fragments arranged by size:
3489 2652 826
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5462 5636 65
Size: 1183 4214 174 1396
Fragments arranged by size:
4214 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5611 5798 1700
Size: 110 3801 187 2869
Fragments arranged by size:
3801 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6967
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 5027 6039 5027
Size: 1012 5955
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6967
BstBI TT'CG_AA
Cuts at: 5771 5771
Size: 6967
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6967
BstYI r'GATC_y
Cuts at: 1972 4123 4297 4664 5049 5066 6518 6604 6616
Size: 2151 174 367 385 17 1452 86 12
Cuts at: 6616 6962 1972
Size: 346 1977
Fragments arranged by size:
2151 1977 1452 385 367 346 174 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2979 2979
Size: 6967
BtgI C'CryG_G
Cuts at: 513 1065 2025 513
Size: 552 960 5455
Fragments arranged by size:
5455 960 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6228
Fragments arranged by size:
6228 683 56
BtsI GCAGTG_nn'
Cuts at: 4483 5277 5297 4483
Size: 794 20 6153
Fragments arranged by size:
6153 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6932
DraI TTT'AAA
Cuts at: 2388 3588 4254 4271 2388
Size: 1200 666 17 5084
Fragments arranged by size:
5084 1200 666 17
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5974 809
Size: 507 4658 1802
Fragments arranged by size:
4658 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3672 4414 5303
Size: 54 1425 50 275 18 1842 742 889
Cuts at: 5303 6932 8
Size: 1629 43
Fragments arranged by size:
1842 1629 1425 889 742 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4414 1537
Size: 275 2602 4090
Fragments arranged by size:
4090 2602 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 2760 4158 4206 4258 4897 1353
Size: 1006 401 1398 48 52 639 3423
Fragments arranged by size:
3423 1398 1006 639 401 52 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5554 5928 6074 387
Size: 1064 138 3965 374 146 1280
Fragments arranged by size:
3965 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2943 3000 3917 4107 5030 5627 6414
Size: 393 1311 57 917 190 923 597 787
Cuts at: 6414 6682 1239
Size: 268 1524
Fragments arranged by size:
1524 1311 923 917 787 597 393 268
190 57
EcoNI CCTnn'n_nnAGG
Cuts at: 4089 4089
Size: 6967
EcoRI G'AATT_C
Cuts at: 2540 4276 4654 2540
Size: 1736 378 4853
Fragments arranged by size:
4853 1736 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4235 4235
Size: 6967
FspI TGC'GCA
Cuts at: 5473 5473
Size: 6967
HaeII r_GCGC'y
Cuts at: 1678 3072 6114 1678
Size: 1394 3042 2531
Fragments arranged by size:
3042 2531 1394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2998 3030 3092
Size: 32 135 32 1025 32 369 32 62
Cuts at: 3092 3124 3539 3571 3575 3607 5608 5640 5682
Size: 32 415 32 4 32 2001 32 42
Cuts at: 5682 5714 1373
Size: 32 2626
Fragments arranged by size:
2626 2001 1025 415 369 135 62 42
32 32 32 32 32 32 32 32
32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2148 4046 4311 4557 669
Size: 988 281 210 1898 265 246 3079
Fragments arranged by size:
3079 1898 988 281 265 246 210
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6967
HpaI GTT'AAC
Cuts at: 2148 4557 2148
Size: 2409 4558
KpnI G_GTAC'C
Cuts at: 2232 4292 2232
Size: 2060 4907
MfeI C'AATT_G
Cuts at: 2428 2514 4566 2428
Size: 86 2052 4829
Fragments arranged by size:
4829 2052 86
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2062 6081 6265 1305
Size: 757 4019 184 2007
Fragments arranged by size:
4019 2007 757 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3674 10
Size: 54 1425 343 1842 3303
Fragments arranged by size:
3303 1842 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4925 5284 5443 518
Size: 648 3759 359 159 2042
Fragments arranged by size:
3759 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 4383 5060 6208 6453 1606
Size: 421 2356 677 1148 245 2120
Fragments arranged by size:
2356 2120 1148 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6415
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6967
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6967
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 2352 4205 4325 5870 1132
Size: 97 1123 1853 120 1545 2229
Fragments arranged by size:
2229 1853 1545 1123 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6967
PciI A'CATG_T
Cuts at: 5866 5866
Size: 6967
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2618 1108
Size: 1510 5457
PfoI T'CCnGG_A
Cuts at: 2800 3016 4084 2800
Size: 216 1068 5683
Fragments arranged by size:
5683 1068 216
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6967
PsiI TTA'TAA
Cuts at: 4537 4537
Size: 6967
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 6967
PstI C_TGCA'G
Cuts at: 830 3631 4319 830
Size: 2801 688 3478
Fragments arranged by size:
3478 2801 688
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
SacII CC_GC'GG
Cuts at: 2028 2028
Size: 6967
SalI G'TCGA_C
Cuts at: 1936 4309 1936
Size: 2373 4594
SapI GCTCTTCn'nnn_
Cuts at: 2359 2359
Size: 6967
SbfI CC_TGCA'GG
Cuts at: 4319 4319
Size: 6967
ScaI AGT'ACT
Cuts at: 5215 6927 5215
Size: 1712 5255
SfcI C'TryA_G
Cuts at: 826 1045 3627 4315 5450 6131 6322 826
Size: 219 2582 688 1135 681 191 1471
Fragments arranged by size:
2582 1471 1135 688 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 4294 1872
Size: 2422 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3849 3881 5071 5972 6234 820
Size: 197 926 1906 32 1190 901 262 1553
Fragments arranged by size:
1906 1553 1190 926 901 262 197 32
SnaBI TAC'GTA
Cuts at: 493 3094 4758 493
Size: 2601 1664 2702
Fragments arranged by size:
2702 2601 1664
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6967
SphI G_CATG'C
Cuts at: 1132 2352 4325 1132
Size: 1220 1973 3774
Fragments arranged by size:
3774 1973 1220
SspI AAT'ATT
Cuts at: 5 52 2531 4891 5
Size: 47 2479 2360 2081
Fragments arranged by size:
2479 2360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6967
StyI C'CwwG_G
Cuts at: 513 1065 3782 4406 513
Size: 552 2717 624 3074
Fragments arranged by size:
3074 2717 624 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3220 4994 5011 5164 5349 5794 325
Size: 1255 1640 1774 17 153 185 445 1498
Fragments arranged by size:
1774 1640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2993 5213 6925
Size: 274 80 33 51 1443 1016 2220 1712
Cuts at: 6925 96
Size: 138
Fragments arranged by size:
2220 1712 1443 1016 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2112 2928 2960 3859 3891 6927 6959 2080
Size: 32 816 32 899 32 3036 32 2088
Fragments arranged by size:
3036 2088 899 816 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 4303 4303
Size: 6967
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6967
XmaI C'CCGG_G
Cuts at: 1870 4292 1870
Size: 2422 4545
XmnI GAAnn'nnTTC
Cuts at: 3190 5096 3190
Size: 1906 5061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2510 4775 276
Size: 53 83 186 936 976 2265 2468
Fragments arranged by size:
2468 2265 976 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI