Restriction map for
pFN21AB0491
(Circular) (Six-base) MAPSORT of: pFN21AB0491.seq Check: 7329 from: 1 to: 6484
pFN21AB0491 6484 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2552 2552
Size: 6484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 3783 4611 4984 1563
Size: 2220 828 373 3063
Fragments arranged by size:
3063 2220 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2896 4547 5931 6199 1632
Size: 1264 1651 1384 268 1917
Fragments arranged by size:
1917 1651 1384 1264 268
AfeI AGC'GCT
Cuts at: 3307 3307
Size: 6484
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 2341 5213 2341
Size: 2872 3612
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3490 1184
Size: 2306 4178
AlwNI CAG_nnn'CTG
Cuts at: 2224 2395 3178 4227 5799 2224
Size: 171 783 1049 1572 2909
Fragments arranged by size:
2909 1572 1049 783 171
ApaI G_GGCC'C
Cuts at: 2782 2782
Size: 6484
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 3793 4019 4171 6280 1493
Size: 2300 226 152 2109 1697
Fragments arranged by size:
2300 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2087 3233 3370 3575 3614 3724
Size: 73 135 9 1146 137 205 39 110
Cuts at: 3724 3809 1870
Size: 85 4545
Fragments arranged by size:
4545 1146 205 137 135 110 85 73
39 9
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3284 3805 5260 618
Size: 325 217 627 1497 521 1455 1842
Fragments arranged by size:
1842 1497 1455 627 521 325 217
BanII G_rGCy'C
Cuts at: 1391 2361 2782 2882 3003 3474 3615 3650 3803
Size: 970 421 100 121 471 141 35 153
Cuts at: 3803 1391
Size: 4072
Fragments arranged by size:
4072 970 471 421 153 141 121 100
35
BbeI G_GCGC'C
Cuts at: 3288 3288
Size: 6484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5513
BbvCI CC'TCA_GC
Cuts at: 3689 3689
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2276 2310 4675 4709 1903
Size: 34 339 34 2365 34 3678
Fragments arranged by size:
3678 2365 339 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BfrBI ATG'CAT
Cuts at: 3673 3673
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2828 2840 3071
Size: 107 122 71 631 605 1156 12 231
Cuts at: 3071 3095 5095 136
Size: 24 2000 1525
Fragments arranged by size:
2000 1525 1156 631 605 231 122 107
71 24 12
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 2279 3731 3901 2279
Size: 1452 170 4862
Fragments arranged by size:
4862 1452 170
Bme1580I G_kGCm'C
Cuts at: 1790 2782 4545 5701 1790
Size: 992 1763 1156 2573
Fragments arranged by size:
2573 1763 1156 992
BmgBI CAC'GTC
Cuts at: 1318 2764 2892 2922 1318
Size: 1446 128 30 4880
Fragments arranged by size:
4880 1446 128 30
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4215 5173 442
Size: 416 53 83 399 24 2798 958 1753
Fragments arranged by size:
2798 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2461 3664 2119
Size: 342 1203 4939
Fragments arranged by size:
4939 1203 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2174 2246 2585 2890 3104 5144 1239
Size: 902 33 72 339 305 214 2040 2579
Fragments arranged by size:
2579 2040 902 339 305 214 72 33
Bpu10I CC'TnA_GC
Cuts at: 3266 3500 3609 3689 3266
Size: 234 109 80 6061
Fragments arranged by size:
6061 234 109 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4609 5474 5772 4609
Size: 865 298 5321
Fragments arranged by size:
5321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 2721 3131 5147 882
Size: 617 585 637 410 2016 2219
Fragments arranged by size:
2219 2016 637 617 585 410
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3259 3285 4291 4673
Size: 53 83 186 936 1726 26 1006 382
Cuts at: 4673 5308 275
Size: 635 1451
Fragments arranged by size:
1726 1451 1006 936 635 382 186 83
53 26
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3416 3434 3446 3464 1178
Size: 30 165 30 2013 18 12 18 4198
Fragments arranged by size:
4198 2013 165 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2279 2729 2822 2825 2828 2831 2942
Size: 1090 3 450 93 3 3 3 111
Cuts at: 2942 1186
Size: 4728
Fragments arranged by size:
4728 1090 450 111 93 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2388 2867 3168 3548 3696 3759 5687 6183 2388
Size: 479 301 380 148 63 1928 496 2689
Fragments arranged by size:
2689 1928 496 479 380 301 148 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2422 2456 3602 1163
Size: 573 686 34 1146 4045
Fragments arranged by size:
4045 1146 686 573 34
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2805 3010 3066 3135 3316 3391
Size: 275 186 804 205 56 69 181 75
Cuts at: 3391 3517 3934 4695 5723 1540
Size: 126 417 761 1028 2301
Fragments arranged by size:
2301 1028 804 761 417 275 205 186
181 126 75 69 56
BsiHKAI G_wGCw'C
Cuts at: 2361 2940 3003 3650 4545 4630 5701 2361
Size: 579 63 647 895 85 1071 3144
Fragments arranged by size:
3144 1071 895 647 579 85 63
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2782 2882 2940 3003 3474 3615
Size: 399 571 421 100 58 63 471 141
Cuts at: 3615 3650 3803 4545 4630 5701 1391
Size: 35 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 471 421 399 153
141 100 85 63 58 35
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2552 3839 844
Size: 1708 1287 3489
Fragments arranged by size:
3489 1708 1287
BsrBI CCG'CTC
Cuts at: 1394 1823 2509 2719 3403 3519 4371 1394
Size: 429 686 210 684 116 852 3507
Fragments arranged by size:
3507 852 686 684 429 210 116
BsrDI GCAATG_nn'
Cuts at: 65 1248 2550 4979 5153 65
Size: 1183 1302 2429 174 1396
Fragments arranged by size:
2429 1396 1302 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2730 3311 5128 5315 1700
Size: 110 920 581 1817 187 2869
Fragments arranged by size:
2869 1817 920 581 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 2019 2137 2139 1925
Size: 94 118 2 6270
Fragments arranged by size:
6270 118 94 2
BssSI C'ACGA_G
Cuts at: 2285 4544 5556 2285
Size: 2259 1012 3213
Fragments arranged by size:
3213 2259 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 2326 1184
Size: 1142 5342
BstXI CCAn_nnnn'nTGG
Cuts at: 2563 3195 2563
Size: 632 5852
BstYI r'GATC_y
Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479
Size: 1842 367 385 17 1452 86 12 346
Cuts at: 6479 1972
Size: 1977
Fragments arranged by size:
1977 1842 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2657 3060 3066 3651 3710 513
Size: 552 939 653 403 6 585 59 3287
Fragments arranged by size:
3287 939 653 585 552 403 59 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 2201 2405 2695 3530 4000 4794 4814 2201
Size: 204 290 835 470 794 20 3871
Fragments arranged by size:
3871 835 794 470 290 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 2431 2431
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2802 3063 3132
Size: 54 1425 50 275 18 972 261 69
Cuts at: 3132 3313 3388 3514 3931 4820 6449 8
Size: 181 75 126 417 889 1629 43
Fragments arranged by size:
1629 1425 972 889 417 275 261 181
126 75 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2802 3063 3132 3313 3388 3514 3931
Size: 275 990 261 69 181 75 126 417
Cuts at: 3931 1537
Size: 4090
Fragments arranged by size:
4090 990 417 275 261 181 126 75
69
EarI CTCTTCn'nnn_
Cuts at: 1353 3008 4414 1353
Size: 1655 1406 3423
Fragments arranged by size:
3423 1655 1406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2602 3127 5071 5445 5591 387
Size: 1064 138 1013 525 1944 374 146 1280
Fragments arranged by size:
1944 1280 1064 1013 525 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 3001 3648 2359
Size: 642 647 5195
Fragments arranged by size:
5195 647 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2174 2246 2585 2890 2896 3104
Size: 393 509 33 72 339 305 6 208
Cuts at: 3104 4547 5144 5931 6199 1239
Size: 1443 597 787 268 1524
Fragments arranged by size:
1524 1443 787 597 509 393 339 305
268 208 72 33 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2416 3452 3752 2416
Size: 1036 300 5148
Fragments arranged by size:
5148 1036 300
EcoO109I rG'GnC_Cy
Cuts at: 3164 3175 3164
Size: 11 6473
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 2048 2177 4990 2048
Size: 129 2813 3542
Fragments arranged by size:
3542 2813 129
HaeII r_GCGC'y
Cuts at: 1678 2919 3288 3309 5631 1678
Size: 1241 369 21 2322 2531
Fragments arranged by size:
2531 2322 1241 369 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 3416 3434 3448
Size: 32 135 32 755 32 1057 18 14
Cuts at: 3448 3466 3493 3525 5125 5157 5199 5231 1373
Size: 18 27 32 1600 32 42 32 2626
Fragments arranged by size:
2626 1600 1057 755 135 42 32 32
32 32 32 32 27 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KasI G'GCGC_C
Cuts at: 3284 3284
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2904 5598 5782 1305
Size: 1599 2694 184 2007
Fragments arranged by size:
2694 2007 1599 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4662
Fragments arranged by size:
4662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4442 4801 4960 518
Size: 648 3276 359 159 2042
Fragments arranged by size:
3276 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 2659 3068 3082 3586 3712 3900 4577
Size: 786 267 409 14 504 126 188 677
Cuts at: 4577 5725 5970 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 786 677 504 409 267 245
188 126 14
NaeI GCC'GGC
Cuts at: 3313 3313
Size: 6484
NarI GG'CG_CC
Cuts at: 3285 3285
Size: 6484
NcoI C'CATG_G
Cuts at: 513 1065 2004 3651 513
Size: 552 939 1647 3346
Fragments arranged by size:
3346 1647 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 3311 3311
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NsiI A_TGCA'T
Cuts at: 3675 3675
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 3842 5387 1132
Size: 97 2613 1545 2229
Fragments arranged by size:
2613 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3454 1348
Size: 2106 4378
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 2224 1108
Size: 1011 105 5368
Fragments arranged by size:
5368 1011 105
PfoI T'CCnGG_A
Cuts at: 2454 2454
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2113 4553 4585 6066 6098 2081
Size: 32 2440 32 1481 32 2467
Fragments arranged by size:
2467 2440 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3164 3164
Size: 6484
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2778 2778
Size: 6484
PstI C_TGCA'G
Cuts at: 830 2028 2208 2400 2495 2690 3332 3836 830
Size: 1198 180 192 95 195 642 504 3478
Fragments arranged by size:
3478 1198 642 504 195 192 180 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2392 3586 2392
Size: 1194 5290
SacI G_AGCT'C
Cuts at: 2361 3003 3650 2361
Size: 642 647 5195
Fragments arranged by size:
5195 647 642
SacII CC_GC'GG
Cuts at: 2660 3069 3713 2660
Size: 409 644 5431
Fragments arranged by size:
5431 644 409
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 3164 3164
Size: 6484
SapI GCTCTTCn'nnn_
Cuts at: 3008 3008
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2024 2204 2362 2396 2491 2686 3328
Size: 219 979 180 158 34 95 195 642
Cuts at: 3328 3832 4967 5648 5839 826
Size: 504 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 681 642 504 219 195
191 180 158 95 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 3095 3095
Size: 6484
SfoI GGC'GCC
Cuts at: 3286 3286
Size: 6484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3235 3372 3811 1872
Size: 1363 137 439 4545
Fragments arranged by size:
4545 1363 439 137
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2087 4588 5489 5751 820
Size: 197 926 135 9 2501 901 262 1553
Fragments arranged by size:
2501 1553 926 901 262 197 135 9
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SrfI GCCC'GGGC
Cuts at: 3372 3372
Size: 6484
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 2861 3659 3704 1517
Size: 1344 798 45 4297
Fragments arranged by size:
4297 1344 798 45
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2556 3054 3636 3651 3753
Size: 552 939 318 234 498 582 15 102
Cuts at: 3753 3923 513
Size: 170 3074
Fragments arranged by size:
3074 939 582 552 498 318 234 170
102 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 2774 3562 4511 4528 4681 4866
Size: 1255 528 666 788 949 17 153 185
Cuts at: 4866 5311 325
Size: 445 1498
Fragments arranged by size:
1498 1255 949 788 666 528 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4730 6442 96
Size: 274 80 33 51 1443 2753 1712 138
Fragments arranged by size:
2753 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6444 6476 6444
Size: 32 6452
Tth111I GACn'n_nGTC
Cuts at: 1316 2534 2962 3118 1316
Size: 1218 428 156 4682
Fragments arranged by size:
4682 1218 428 156
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3634 3067
Size: 567 5917
XhoI C'TCGA_G
Cuts at: 1943 2078 2087 1943
Size: 135 9 6340
Fragments arranged by size:
6340 135 9
XmaI C'CCGG_G
Cuts at: 1870 3233 3370 3809 1870
Size: 1363 137 439 4545
Fragments arranged by size:
4545 1363 439 137
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NruI PacI PmlI PspXI
PsrI RsrII SexAI SwaI